Restriction map for
pF1KB6217
(Circular) (Six-base) MAPSORT of: pF1KB6217.seq Check: 7097 from: 1 to: 5297
pF1KB6217 5297 bp
With 182 enzymes: SgfI *
October 7, 2009 14:20 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1515 1515
Size: 5297
AccI GT'mk_AC
Cuts at: 2335 2335
Size: 5297
Acc65I G'GTAC_C
Cuts at: 701 1037 2313 701
Size: 336 1276 3685
Fragments arranged by size:
3685 1276 336
AclI AA'CG_TT
Cuts at: 5137 5137
Size: 5297
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 597 3122 3554 4301 4569 597
Size: 2525 432 747 268 1325
Fragments arranged by size:
2525 1325 747 432 268
AflIII A'CryG_T
Cuts at: 2649 3753 4730 2649
Size: 1104 977 3216
Fragments arranged by size:
3216 1104 977
AgeI A'CCGG_T
Cuts at: 3685 3685
Size: 5297
AleI CACnn'nnGTG
Cuts at: 793 793
Size: 5297
AlwNI CAG_nnn'CTG
Cuts at: 375 855 1324 1640 2176 2602 4169 375
Size: 480 469 316 536 426 1567 1503
Fragments arranged by size:
1567 1503 536 480 469 426 316
ApaI G_GGCC'C
Cuts at: 172 382 885 1332 2230 2291 172
Size: 210 503 447 898 61 3178
Fragments arranged by size:
3178 898 503 447 210 61
ApaLI G'TGCA_C
Cuts at: 1342 4067 1342
Size: 2725 2572
ApoI r'AATT_y
Cuts at: 2301 4650 4906 2301
Size: 2349 256 2692
Fragments arranged by size:
2692 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5297
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5297
AvaI C'yCGr_G
Cuts at: 134 1331 2317 134
Size: 1197 986 3114
Fragments arranged by size:
3114 1197 986
BamHI G'GATC_C
Cuts at: 2322 2322
Size: 5297
BanI G'GyrC_C
Cuts at: 393 493 701 1037 2313 2727 2975 3010 393
Size: 100 208 336 1276 414 248 35 2680
Fragments arranged by size:
2680 1276 414 336 248 208 100 35
BanII G_rGCy'C
Cuts at: 172 179 224 344 382 458 885 894 1332
Size: 7 45 120 38 76 427 9 438
Cuts at: 1332 2000 2230 2251 2291 2311 3341 172
Size: 668 230 21 40 20 1030 2128
Fragments arranged by size:
2128 1030 668 438 427 230 120 76
45 40 38 21 20 9 7
BbeI G_GCGC'C
Cuts at: 397 497 2731 2979 397
Size: 100 2234 248 2715
Fragments arranged by size:
2715 2234 248 100
BbsI GAAGACnn'nnnn_
Cuts at: 538 654 1232 1782 538
Size: 116 578 550 4053
Fragments arranged by size:
4053 578 550 116
BciVI GTATCCnnnnn_n'
Cuts at: 3195 3956 5297 3195
Size: 761 1341 3195
Fragments arranged by size:
3195 1341 761
BclI T'GATC_A
Cuts at: 1559 2817 1559
Size: 1258 4039
BglI GCCn_nnn'nGGC
Cuts at: 544 2078 544
Size: 1534 3763
BglII A'GATC_T
Cuts at: 2812 4849 2812
Size: 2037 3260
BlpI GC'TnA_GC
Cuts at: 178 1994 2409 178
Size: 1816 415 3066
Fragments arranged by size:
3066 1816 415
Bme1580I G_kGCm'C
Cuts at: 163 172 382 885 1332 1346 2230 2291 2922
Size: 9 210 503 447 14 884 61 631
Cuts at: 2922 3015 4071 163
Size: 93 1056 1389
Fragments arranged by size:
1389 1056 884 631 503 447 210 93
61 14 9
BmrI ACTGGGnnnn_n'
Cuts at: 1334 2509 2590 2925 5069 1334
Size: 1175 81 335 2144 1562
Fragments arranged by size:
2144 1562 1175 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 330 1920 2118 3664 330
Size: 1590 198 1546 1963
Fragments arranged by size:
1963 1590 1546 198
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 262 320 322 745 891 1114 1648 2051 2171
Size: 58 2 423 146 223 534 403 120
Cuts at: 2171 2225 262
Size: 54 3334
Fragments arranged by size:
3334 534 423 403 223 146 120 58
54 2
Bpu10I CC'TnA_GC
Cuts at: 2090 2090
Size: 5297
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2473 3352 3844 4142 2473
Size: 879 492 298 3628
Fragments arranged by size:
3628 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 286 1149 1399 2069 5001 286
Size: 863 250 670 2932 582
Fragments arranged by size:
2932 863 670 582 250
BsaAI yAC'GTr
Cuts at: 2650 2650
Size: 5297
BsaBI GATnn'nnATC
Cuts at: 6 2811 4848 6
Size: 2805 2037 455
Fragments arranged by size:
2805 2037 455
BsaHI Gr'CG_yC
Cuts at: 394 494 2728 2976 3678 5174 394
Size: 100 2234 248 702 1496 517
Fragments arranged by size:
2234 1496 702 517 248 100
BsaWI w'CCGG_w
Cuts at: 2486 3007 3685 3959 4106 4517 2486
Size: 521 678 274 147 411 3266
Fragments arranged by size:
3266 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1129 1159 2187 2217 1129
Size: 30 1028 30 4209
Fragments arranged by size:
4209 1028 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 259 319 379 430 433 733 742 1111 1668
Size: 60 60 51 3 300 9 369 557
Cuts at: 1668 1956 259
Size: 288 3600
Fragments arranged by size:
3600 557 369 300 288 60 60 51
9 3
BseYI C'CCAG_C
Cuts at: 375 398 515 1107 2165 2722 3480 4057 4553
Size: 23 117 592 1058 557 758 577 496
Cuts at: 4553 375
Size: 1119
Fragments arranged by size:
1119 1058 758 592 577 557 496 117
23
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 546 666 1449 546
Size: 120 783 4394
Fragments arranged by size:
4394 783 120
BsiEI CG_ry'CG
Cuts at: 104 2839 2885 4093 104
Size: 2735 46 1208 1308
Fragments arranged by size:
2735 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 179 458 514 1346 2000 2091 2311 3089 3279
Size: 279 56 832 654 91 220 778 190
Cuts at: 3279 4071 179
Size: 792 1405
Fragments arranged by size:
1405 832 792 778 654 279 220 190
91 56
Bsp1286I G_dGCh'C
Cuts at: 163 172 179 224 344 382 458 514 885
Size: 9 7 45 120 38 76 56 371
Cuts at: 885 894 1332 1346 2000 2091 2230 2251 2291
Size: 9 438 14 654 91 139 21 40
Cuts at: 2291 2311 2922 3015 3089 3279 3341 4071 163
Size: 20 611 93 74 190 62 730 1389
Fragments arranged by size:
1389 730 654 611 438 371 190 139
120 93 91 76 74 62 56 45
40 38 21 20 14 9 9 7
BspEI T'CCGG_A
Cuts at: 4517 4517
Size: 5297
BspHI T'CATG_A
Cuts at: 224 344 4473 224
Size: 120 4129 1048
Fragments arranged by size:
4129 1048 120
BspMI ACCTGCnnnn'nnnn_
Cuts at: 396 830 1368 1515 2018 2347 2561 2863 3244
Size: 434 538 147 503 329 214 302 381
Cuts at: 3244 396
Size: 2449
Fragments arranged by size:
2449 538 503 434 381 329 302 214
147
BsrBI CCG'CTC
Cuts at: 50 443 3589 3643 5127 5292 50
Size: 393 3146 54 1484 165 55
Fragments arranged by size:
3146 1484 393 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3209 3209
Size: 5297
BsrFI r'CCGG_y
Cuts at: 799 3295 3476 3685 799
Size: 2496 181 209 2411
Fragments arranged by size:
2496 2411 209 181
BsrGI T'GTAC_A
Cuts at: 767 767
Size: 5297
BssHII G'CGCG_C
Cuts at: 1281 1281
Size: 5297
BssSI C'ACGA_G
Cuts at: 793 3568 3926 793
Size: 2775 358 2164
Fragments arranged by size:
2775 2164 358
BstAPI GCAn_nnn'nTGC
Cuts at: 846 2674 846
Size: 1828 3469
BstBI TT'CG_AA
Cuts at: 3658 3658
Size: 5297
BstEII G'GTnAC_C
Cuts at: 1917 1917
Size: 5297
BstXI CCAn_nnnn'nTGG
Cuts at: 114 504 795 114
Size: 390 291 4616
Fragments arranged by size:
4616 390 291
BstYI r'GATC_y
Cuts at: 1 2322 2789 2812 3147 3393 4405 4491 4503
Size: 2321 467 23 335 246 1012 86 12
Cuts at: 4503 4849 1
Size: 346 449
Fragments arranged by size:
2321 1012 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 856 856
Size: 5297
BtgI C'CryG_G
Cuts at: 107 407 489 1594 107
Size: 300 82 1105 3810
Fragments arranged by size:
3810 1105 300 82
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3247 3428 3591 3247
Size: 181 163 4953
Fragments arranged by size:
4953 181 163
BtsI GCAGTG_nn'
Cuts at: 554 677 1485 2540 554
Size: 123 808 1055 3311
Fragments arranged by size:
3311 1055 808 123
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1551 1586 1551
Size: 35 5262
DraI TTT'AAA
Cuts at: 2296 2296
Size: 5297
DraIII CAC_nnn'GTG
Cuts at: 1706 1706
Size: 5297
DrdI GACnn_nn'nnGTC
Cuts at: 658 2014 3003 3861 658
Size: 1356 989 858 2094
Fragments arranged by size:
2094 1356 989 858
EaeI y'GGCC_r
Cuts at: 116 797 1847 2882 3056 3447 3474 4819 116
Size: 681 1050 1035 174 391 27 1345 594
Fragments arranged by size:
1345 1050 1035 681 594 391 174 27
EagI C'GGCC_G
Cuts at: 2882 2882
Size: 5297
EarI CTCTTCn'nnn_
Cuts at: 463 3320 3530 463
Size: 2857 210 2230
Fragments arranged by size:
2857 2230 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 427 490 2259 3505 3815 3961 5107 427
Size: 63 1769 1246 310 146 1146 617
Fragments arranged by size:
1769 1246 1146 617 310 146 63
EcoICRI GAG'CTC
Cuts at: 177 456 1998 2309 177
Size: 279 1542 311 3165
Fragments arranged by size:
3165 1542 311 279
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 262 320 322 597 745 891 1114 1648 2051
Size: 58 2 275 148 146 223 534 403
Cuts at: 2051 2171 2225 3122 3554 4301 4569 262
Size: 120 54 897 432 747 268 990
Fragments arranged by size:
990 897 747 534 432 403 275 268
223 148 146 120 58 54 2
EcoNI CCTnn'n_nnAGG
Cuts at: 1096 1096
Size: 5297
EcoO109I rG'GnC_Cy
Cuts at: 168 252 379 423 861 881 1328 2173 2227
Size: 84 127 44 438 20 447 845 54
Cuts at: 2227 2287 2436 168
Size: 60 149 3029
Fragments arranged by size:
3029 845 447 438 149 127 84 60
54 44 20
EcoRI G'AATT_C
Cuts at: 2301 2301
Size: 5297
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 555 678 975 3316 555
Size: 123 297 2341 2536
Fragments arranged by size:
2536 2341 297 123
FspI TGC'GCA
Cuts at: 3078 3078
Size: 5297
HaeII r_GCGC'y
Cuts at: 397 497 855 2731 2979 4001 4980 397
Size: 100 358 1876 248 1022 979 714
Fragments arranged by size:
1876 1022 979 714 358 248 100
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 184 216 1663 1695 2187 2219 3139 3169 3171
Size: 32 1447 32 492 32 920 30 2
Cuts at: 3171 3201 184
Size: 30 2280
Fragments arranged by size:
2280 1447 920 492 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1671 1827 1857 2336 1671
Size: 156 30 479 4632
Fragments arranged by size:
4632 479 156 30
KasI G'GCGC_C
Cuts at: 393 493 2727 2975 393
Size: 100 2234 248 2715
Fragments arranged by size:
2715 2234 248 100
KpnI G_GTAC'C
Cuts at: 705 1041 2317 705
Size: 336 1276 3685
Fragments arranged by size:
3685 1276 336
MluI A'CGCG_T
Cuts at: 4730 4730
Size: 5297
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1250 1955 3968 4152 1250
Size: 705 2013 184 2395
Fragments arranged by size:
2395 2013 705 184
MscI TGG'CCA
Cuts at: 118 1849 3058 118
Size: 1731 1209 2357
Fragments arranged by size:
2357 1731 1209
MslI CAynn'nnrTG
Cuts at: 112 793 1417 3286 3413 112
Size: 681 624 1869 127 1996
Fragments arranged by size:
1996 1869 681 624 127
MspA1I CmG'CkG
Cuts at: 966 1596 2408 2722 3082 4095 4340 966
Size: 630 812 314 360 1013 245 1923
Fragments arranged by size:
1923 1013 812 630 360 314 245
NaeI GCC'GGC
Cuts at: 801 3478 801
Size: 2677 2620
NarI GG'CG_CC
Cuts at: 394 494 2728 2976 394
Size: 100 2234 248 2715
Fragments arranged by size:
2715 2234 248 100
NcoI C'CATG_G
Cuts at: 107 107
Size: 5297
NgoMIV G'CCGG_C
Cuts at: 799 3476 799
Size: 2677 2620
NruI TCG'CGA
Cuts at: 818 1806 818
Size: 988 4309
NspI r_CATG'y
Cuts at: 2350 2850 3757 2350
Size: 500 907 3890
Fragments arranged by size:
3890 907 500
PasI CC'CwG_GG
Cuts at: 2223 2223
Size: 5297
PciI A'CATG_T
Cuts at: 3753 3753
Size: 5297
PflMI CCAn_nnn'nTGG
Cuts at: 1232 1608 1232
Size: 376 4921
PfoI T'CCnGG_A
Cuts at: 1547 1547
Size: 5297
PmeI GTTT'AAAC
Cuts at: 2296 2296
Size: 5297
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4436 4468 4436
Size: 32 5265
PpuMI rG'GwC_Cy
Cuts at: 252 861 252
Size: 609 4688
PspOMI G'GGCC_C
Cuts at: 168 378 881 1328 2226 2287 168
Size: 210 503 447 898 61 3178
Fragments arranged by size:
3178 898 503 447 210 61
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 1597 1629 1597
Size: 32 5265
PstI C_TGCA'G
Cuts at: 269 2256 2344 3029 269
Size: 1987 88 685 2537
Fragments arranged by size:
2537 1987 685 88
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5297
PvuII CAG'CTG
Cuts at: 966 2722 3082 966
Size: 1756 360 3181
Fragments arranged by size:
3181 1756 360
RsrII CG'GwC_CG
Cuts at: 3492 3492
Size: 5297
SacI G_AGCT'C
Cuts at: 179 458 2000 2311 179
Size: 279 1542 311 3165
Fragments arranged by size:
3165 1542 311 279
SacII CC_GC'GG
Cuts at: 1597 1597
Size: 5297
SalI G'TCGA_C
Cuts at: 2334 2334
Size: 5297
SanDI GG'GwC_CC
Cuts at: 861 861
Size: 5297
SapI GCTCTTCn'nnn_
Cuts at: 463 3320 3530 463
Size: 2857 210 2230
Fragments arranged by size:
2857 2230 210
SbfI CC_TGCA'GG
Cuts at: 2344 2344
Size: 5297
ScaI AGT'ACT
Cuts at: 610 1615 4814 610
Size: 1005 3199 1093
Fragments arranged by size:
3199 1093 1005
SexAI A'CCwGG_T
Cuts at: 276 1139 276
Size: 863 4434
SfcI C'TryA_G
Cuts at: 33 265 696 1623 2252 2340 3025 4018 4209
Size: 232 431 927 629 88 685 993 191
Cuts at: 4209 33
Size: 1121
Fragments arranged by size:
1121 993 927 685 629 431 232 191
88
SfoI GGC'GCC
Cuts at: 395 495 2729 2977 395
Size: 100 2234 248 2715
Fragments arranged by size:
2715 2234 248 100
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5297
SmaI CCC'GGG
Cuts at: 1333 2319 1333
Size: 986 4311
SmlI C'TyrA_G
Cuts at: 2452 3367 3859 4121 2452
Size: 915 492 262 3628
Fragments arranged by size:
3628 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5297
SphI G_CATG'C
Cuts at: 2350 2850 2350
Size: 500 4797
StyI C'CwwG_G
Cuts at: 107 2431 2785 107
Size: 2324 354 2619
Fragments arranged by size:
2619 2324 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2880 3013 3472 3681 2880
Size: 133 459 209 4496
Fragments arranged by size:
4496 459 209 133
TatI w'GTAC_w
Cuts at: 578 608 767 1514 1613 4812 578
Size: 30 159 747 99 3199 1063
Fragments arranged by size:
3199 1063 747 159 99 30
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 123 155 345 377 675 707 4814 4846 123
Size: 32 190 32 298 32 4107 32 574
Fragments arranged by size:
4107 574 298 190 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 3094 3094
Size: 5297
XbaI T'CTAG_A
Cuts at: 2328 2328
Size: 5297
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1186 1744 2792 1186
Size: 558 1048 3691
Fragments arranged by size:
3691 1048 558
XmaI C'CCGG_G
Cuts at: 1331 2317 1331
Size: 986 4311
XmnI GAAnn'nnTTC
Cuts at: 1297 1297
Size: 5297
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BciVI BclI BglI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NruI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PspOMI PsrI PstI PvuI PvuII
RsrII SacI SacII SalI SanDI SapI SbfI ScaI
SexAI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AlfI AloI AscI AvrII
BaeI BbvCI BcgI BfrBI BmgBI BmtI BsiWI BsmI
BsmBI BstZ17I ClaI EcoRV FseI FspAI HindIII HpaI
MfeI NdeI NheI NotI NsiI PacI PmlI PshAI
PsiI PspXI SfiI SgrAI SnaBI SrfI SspI StuI
SwaI XhoI ZraI