(Circular) (Six-base) MAPSORT of: pF1KB6164.seq Check: 9892 from: 1 to: 4919 pF1KB6164 4919 bp With 182 enzymes: SgfI * October 7, 2009 14:19 .. AatII G_ACGT'C Cuts at: 1277 1277 Size: 4919 AccI GT'mk_AC Cuts at: 1121 1957 1121 Size: 836 4083 Acc65I G'GTAC_C Cuts at: 1935 1935 Size: 4919 AclI AA'CG_TT Cuts at: 4759 4759 Size: 4919 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1537 1768 2744 3176 3923 4191 1537 Size: 231 976 432 747 268 2265 Fragments arranged by size: 2265 976 747 432 268 231 AflIII A'CryG_T Cuts at: 1841 2271 3375 4352 1841 Size: 430 1104 977 2408 Fragments arranged by size: 2408 1104 977 430 AgeI A'CCGG_T Cuts at: 3307 3307 Size: 4919 AhdI GACnn_n'nnGTC Cuts at: 501 501 Size: 4919 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 1228 1260 1301 1333 1228 Size: 32 41 32 4814 Fragments arranged by size: 4814 41 32 32 AlwNI CAG_nnn'CTG Cuts at: 682 1545 1746 2224 3791 682 Size: 863 201 478 1567 1810 Fragments arranged by size: 1810 1567 863 478 201 ApaI G_GGCC'C Cuts at: 341 341 Size: 4919 ApaLI G'TGCA_C Cuts at: 3689 3689 Size: 4919 ApoI r'AATT_y Cuts at: 526 1923 4272 4528 526 Size: 1397 2349 256 917 Fragments arranged by size: 2349 1397 917 256 AseI AT'TA_AT Cuts at: 20 20 Size: 4919 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 4919 AvaI C'yCGr_G Cuts at: 177 863 1427 1939 177 Size: 686 564 512 3157 Fragments arranged by size: 3157 686 564 512 BamHI G'GATC_C Cuts at: 858 1944 858 Size: 1086 3833 BanI G'GyrC_C Cuts at: 1935 2349 2597 2632 1935 Size: 414 248 35 4222 Fragments arranged by size: 4222 414 248 35 BanII G_rGCy'C Cuts at: 140 341 710 923 1007 1680 1933 2963 140 Size: 201 369 213 84 673 253 1030 2096 Fragments arranged by size: 2096 1030 673 369 253 213 201 84 BbeI G_GCGC'C Cuts at: 2353 2601 2353 Size: 248 4671 BbvCI CC'TCA_GC Cuts at: 388 1130 1730 388 Size: 742 600 3577 Fragments arranged by size: 3577 742 600 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 90 124 90 Size: 34 4885 BciVI GTATCCnnnnn_n' Cuts at: 1673 2817 3578 4919 1673 Size: 1144 761 1341 1673 Fragments arranged by size: 1673 1341 1144 761 BclI T'GATC_A Cuts at: 2439 2439 Size: 4919 BglII A'GATC_T Cuts at: 2434 4471 2434 Size: 2037 2882 BlpI GC'TnA_GC Cuts at: 313 1001 2031 313 Size: 688 1030 3201 Fragments arranged by size: 3201 1030 688 Bme1580I G_kGCm'C Cuts at: 341 2544 2637 3693 341 Size: 2203 93 1056 1567 Fragments arranged by size: 2203 1567 1056 93 BmgBI CAC'GTC Cuts at: 1842 1842 Size: 4919 BmrI ACTGGGnnnn_n' Cuts at: 246 1653 2131 2212 2547 4691 246 Size: 1407 478 81 335 2144 474 Fragments arranged by size: 2144 1407 478 474 335 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3286 3286 Size: 4919 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 721 1030 1180 1279 1882 721 Size: 309 150 99 603 3758 Fragments arranged by size: 3758 603 309 150 99 Bpu10I CC'TnA_GC Cuts at: 388 1130 1730 388 Size: 742 600 3577 Fragments arranged by size: 3577 742 600 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 790 865 1189 2095 2974 3466 3764 790 Size: 75 324 906 879 492 298 1945 Fragments arranged by size: 1945 906 879 492 324 298 75 BsaI GGTCTCn'nnnn_ Cuts at: 1711 4623 1711 Size: 2912 2007 BsaAI yAC'GTr Cuts at: 2272 2272 Size: 4919 BsaBI GATnn'nnATC Cuts at: 6 2433 4470 6 Size: 2427 2037 455 Fragments arranged by size: 2427 2037 455 BsaHI Gr'CG_yC Cuts at: 1274 2350 2598 3300 4796 1274 Size: 1076 248 702 1496 1397 Fragments arranged by size: 1496 1397 1076 702 248 BsaWI w'CCGG_w Cuts at: 647 2108 2629 3307 3581 3728 4139 647 Size: 1461 521 678 274 147 411 1427 Fragments arranged by size: 1461 1427 678 521 411 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 442 472 818 848 1228 1258 1450 1480 442 Size: 30 346 30 380 30 192 30 3881 Fragments arranged by size: 3881 380 346 192 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 223 363 466 1276 1651 223 Size: 140 103 810 375 3491 Fragments arranged by size: 3491 810 375 140 103 BseYI C'CCAG_C Cuts at: 215 227 233 239 283 2344 3102 3679 4175 Size: 12 6 6 44 2061 758 577 496 Cuts at: 4175 215 Size: 959 Fragments arranged by size: 2061 959 758 577 496 44 12 6 6 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1436 1542 1436 Size: 106 4813 BsiEI CG_ry'CG Cuts at: 104 2461 2507 3715 104 Size: 2357 46 1208 1308 Fragments arranged by size: 2357 1308 1208 46 BsiHKAI G_wGCw'C Cuts at: 1007 1064 1374 1933 2711 2901 3693 1007 Size: 57 310 559 778 190 792 2233 Fragments arranged by size: 2233 792 778 559 310 190 57 BsmBI CGTCTCn'nnnn_ Cuts at: 970 970 Size: 4919 Bsp1286I G_dGCh'C Cuts at: 140 341 710 923 1007 1064 1374 1680 1933 Size: 201 369 213 84 57 310 306 253 Cuts at: 1933 2544 2637 2711 2901 2963 3693 140 Size: 611 93 74 190 62 730 1366 Fragments arranged by size: 1366 730 611 369 310 306 253 213 201 190 93 84 74 62 57 BspEI T'CCGG_A Cuts at: 647 4139 647 Size: 3492 1427 BspHI T'CATG_A Cuts at: 1400 4095 1400 Size: 2695 2224 BspMI ACCTGCnnnn'nnnn_ Cuts at: 1969 2183 2485 2866 1969 Size: 214 302 381 4022 Fragments arranged by size: 4022 381 302 214 BsrBI CCG'CTC Cuts at: 50 447 870 3211 3265 4749 4914 50 Size: 397 423 2341 54 1484 165 55 Fragments arranged by size: 2341 1484 423 397 165 55 54 BsrDI GCAATG_nn' Cuts at: 2831 2831 Size: 4919 BsrFI r'CCGG_y Cuts at: 1185 1592 2917 3098 3307 1185 Size: 407 1325 181 209 2797 Fragments arranged by size: 2797 1325 407 209 181 BsrGI T'GTAC_A Cuts at: 1844 1844 Size: 4919 BssSI C'ACGA_G Cuts at: 742 3190 3548 742 Size: 2448 358 2113 Fragments arranged by size: 2448 2113 358 BstAPI GCAn_nnn'nTGC Cuts at: 1369 2296 1369 Size: 927 3992 BstBI TT'CG_AA Cuts at: 3280 3280 Size: 4919 BstYI r'GATC_y Cuts at: 1 858 1013 1944 2411 2434 2769 3015 4027 Size: 857 155 931 467 23 335 246 1012 Cuts at: 4027 4113 4125 4471 1 Size: 86 12 346 449 Fragments arranged by size: 1012 931 857 467 449 346 335 246 155 86 23 12 Bsu36I CC'TnA_GG Cuts at: 614 614 Size: 4919 BtgI C'CryG_G Cuts at: 1494 1494 Size: 4919 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 880 2869 3050 3213 880 Size: 1989 181 163 2586 Fragments arranged by size: 2586 1989 181 163 BtsI GCAGTG_nn' Cuts at: 1687 2162 1687 Size: 475 4444 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 171 206 171 Size: 35 4884 DraI TTT'AAA Cuts at: 1918 1918 Size: 4919 DraIII CAC_nnn'GTG Cuts at: 517 517 Size: 4919 DrdI GACnn_nn'nnGTC Cuts at: 2625 3483 2625 Size: 858 4061 EaeI y'GGCC_r Cuts at: 727 1328 2504 2678 3069 3096 4441 727 Size: 601 1176 174 391 27 1345 1205 Fragments arranged by size: 1345 1205 1176 601 391 174 27 EagI C'GGCC_G Cuts at: 2504 2504 Size: 4919 EarI CTCTTCn'nnn_ Cuts at: 649 1052 1321 1610 1744 1877 2942 3152 649 Size: 403 269 289 134 133 1065 210 2416 Fragments arranged by size: 2416 1065 403 289 269 210 134 133 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 1309 1894 3127 3437 3583 4729 1309 Size: 585 1233 310 146 1146 1499 Fragments arranged by size: 1499 1233 1146 585 310 146 EcoICRI GAG'CTC Cuts at: 1005 1931 1005 Size: 926 3993 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 721 1030 1180 1279 1537 1768 1882 2744 3176 Size: 309 150 99 258 231 114 862 432 Cuts at: 3176 3923 4191 721 Size: 747 268 1449 Fragments arranged by size: 1449 862 747 432 309 268 258 231 150 114 99 EcoNI CCTnn'n_nnAGG Cuts at: 1099 1162 1099 Size: 63 4856 EcoO109I rG'GnC_Cy Cuts at: 337 338 2058 337 Size: 1 1720 3198 Fragments arranged by size: 3198 1720 1 EcoRI G'AATT_C Cuts at: 1923 1923 Size: 4919 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2938 2938 Size: 4919 FspI TGC'GCA Cuts at: 2700 2700 Size: 4919 HaeII r_GCGC'y Cuts at: 2353 2601 3623 4602 2353 Size: 248 1022 979 2670 Fragments arranged by size: 2670 1022 979 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 487 519 969 1001 2761 2791 2793 2823 487 Size: 32 450 32 1760 30 2 30 2583 Fragments arranged by size: 2583 1760 450 32 32 30 30 2 HincII GTy'rAC Cuts at: 1958 1958 Size: 4919 HindIII A'AGCT_T Cuts at: 412 412 Size: 4919 KasI G'GCGC_C Cuts at: 2349 2597 2349 Size: 248 4671 KpnI G_GTAC'C Cuts at: 1939 1939 Size: 4919 MluI A'CGCG_T Cuts at: 4352 4352 Size: 4919 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 296 1805 3590 3774 296 Size: 1509 1785 184 1441 Fragments arranged by size: 1785 1509 1441 184 MscI TGG'CCA Cuts at: 729 1330 2680 729 Size: 601 1350 2968 Fragments arranged by size: 2968 1350 601 MslI CAynn'nnrTG Cuts at: 584 2908 3035 584 Size: 2324 127 2468 Fragments arranged by size: 2468 2324 127 MspA1I CmG'CkG Cuts at: 1134 1326 1729 2030 2344 2704 3717 3962 1134 Size: 192 403 301 314 360 1013 245 2091 Fragments arranged by size: 2091 1013 403 360 314 301 245 192 NaeI GCC'GGC Cuts at: 3100 3100 Size: 4919 NarI GG'CG_CC Cuts at: 2350 2598 2350 Size: 248 4671 NcoI C'CATG_G Cuts at: 1494 1494 Size: 4919 NgoMIV G'CCGG_C Cuts at: 3098 3098 Size: 4919 NspI r_CATG'y Cuts at: 1972 2472 3379 1972 Size: 500 907 3512 Fragments arranged by size: 3512 907 500 PciI A'CATG_T Cuts at: 3375 3375 Size: 4919 PfoI T'CCnGG_A Cuts at: 1007 1157 1007 Size: 150 4769 PmeI GTTT'AAAC Cuts at: 1918 1918 Size: 4919 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 1228 1260 4058 4090 1228 Size: 32 2798 32 2057 Fragments arranged by size: 2798 2057 32 32 PspOMI G'GGCC_C Cuts at: 337 337 Size: 4919 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 1378 1410 1654 1686 1378 Size: 32 244 32 4611 Fragments arranged by size: 4611 244 32 32 PstI C_TGCA'G Cuts at: 410 1966 2651 410 Size: 1556 685 2678 Fragments arranged by size: 2678 1556 685 PvuI CG_AT'CG Cuts at: 104 104 Size: 4919 PvuII CAG'CTG Cuts at: 1134 1326 1729 2344 2704 1134 Size: 192 403 615 360 3349 Fragments arranged by size: 3349 615 403 360 192 RsrII CG'GwC_CG Cuts at: 3114 3114 Size: 4919 SacI G_AGCT'C Cuts at: 1007 1933 1007 Size: 926 3993 SalI G'TCGA_C Cuts at: 1956 1956 Size: 4919 SapI GCTCTTCn'nnn_ Cuts at: 1052 2942 3152 1052 Size: 1890 210 2819 Fragments arranged by size: 2819 1890 210 SbfI CC_TGCA'GG Cuts at: 1966 1966 Size: 4919 ScaI AGT'ACT Cuts at: 328 4436 328 Size: 4108 811 SfcI C'TryA_G Cuts at: 33 406 960 1458 1962 2647 3640 3831 33 Size: 373 554 498 504 685 993 191 1121 Fragments arranged by size: 1121 993 685 554 504 498 373 191 SfoI GGC'GCC Cuts at: 2351 2599 2351 Size: 248 4671 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 4919 SmaI CCC'GGG Cuts at: 1941 1941 Size: 4919 SmlI C'TyrA_G Cuts at: 769 863 880 1204 2074 2989 3481 3743 769 Size: 94 17 324 870 915 492 262 1945 Fragments arranged by size: 1945 915 870 492 324 262 94 17 SpeI A'CTAG_T Cuts at: 67 67 Size: 4919 SphI G_CATG'C Cuts at: 1972 2472 1972 Size: 500 4419 StyI C'CwwG_G Cuts at: 143 1232 1494 2053 2407 143 Size: 1089 262 559 354 2655 Fragments arranged by size: 2655 1089 559 354 262 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 592 2502 2635 3094 3303 592 Size: 1910 133 459 209 2208 Fragments arranged by size: 2208 1910 459 209 133 TatI w'GTAC_w Cuts at: 326 569 806 825 1394 1700 1844 4434 326 Size: 243 237 19 569 306 144 2590 811 Fragments arranged by size: 2590 811 569 306 243 237 144 19 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 1606 1638 4436 4468 1606 Size: 32 2798 32 2057 Fragments arranged by size: 2798 2057 32 32 Tth111I GACn'n_nGTC Cuts at: 2716 2716 Size: 4919 XbaI T'CTAG_A Cuts at: 1950 1950 Size: 4919 XcmI CCAnnnn_n'nnnnTGG Cuts at: 1857 2414 1857 Size: 557 4362 XhoI C'TCGA_G Cuts at: 863 863 Size: 4919 XmaI C'CCGG_G Cuts at: 1939 1939 Size: 4919 XmnI GAAnn'nnTTC Cuts at: 643 643 Size: 4919 ZraI GAC'GTC Cuts at: 1275 1275 Size: 4919 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflIII AgeI AhdI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbvCI BcgI BciVI BclI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstYI Bsu36I BtgI BtgZI BtsI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI PfoI PmeI PpiI PspOMI PsrI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AflII AleI AlfI AscI AvrII BaeI BbsI BfrBI BglI BmtI BsiWI BsmI BssHII BstEII BstXI BstZ17I ClaI EcoRV FseI FspAI HpaI MfeI NdeI NheI NotI NruI NsiI PacI PasI PflMI PmlI PpuMI PshAI PsiI PspXI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI