Restriction map for pF1KB6164
 (Circular) (Six-base) MAPSORT of: pF1KB6164.seq  Check: 9892  from: 1  to: 4919



pF1KB6164  4919 bp

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:19  ..

AatII G_ACGT'C

Cuts at:   1277    1277 
   Size:       4919
 
AccI GT'mk_AC

Cuts at:   1121    1957    1121 
   Size:        836    4083
 
Acc65I G'GTAC_C

Cuts at:   1935    1935 
   Size:       4919
 
AclI AA'CG_TT

Cuts at:   4759    4759 
   Size:       4919
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1537    1768    2744    3176    3923    4191    1537 
   Size:        231     976     432     747     268    2265

  Fragments arranged by size: 

                2265     976     747     432     268     231
 
AflIII A'CryG_T

Cuts at:   1841    2271    3375    4352    1841 
   Size:        430    1104     977    2408

  Fragments arranged by size: 

                2408    1104     977     430
 
AgeI A'CCGG_T

Cuts at:   3307    3307 
   Size:       4919
 
AhdI GACnn_n'nnGTC

Cuts at:    501     501 
   Size:       4919
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   1228    1260    1301    1333    1228 
   Size:         32      41      32    4814

  Fragments arranged by size: 

                4814      41      32      32
 
AlwNI CAG_nnn'CTG

Cuts at:    682    1545    1746    2224    3791     682 
   Size:        863     201     478    1567    1810

  Fragments arranged by size: 

                1810    1567     863     478     201
 
ApaI G_GGCC'C

Cuts at:    341     341 
   Size:       4919
 
ApaLI G'TGCA_C

Cuts at:   3689    3689 
   Size:       4919
 
ApoI r'AATT_y

Cuts at:    526    1923    4272    4528     526 
   Size:       1397    2349     256     917

  Fragments arranged by size: 

                2349    1397     917     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       4919
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4919
 
AvaI C'yCGr_G

Cuts at:    177     863    1427    1939     177 
   Size:        686     564     512    3157

  Fragments arranged by size: 

                3157     686     564     512
 
BamHI G'GATC_C

Cuts at:    858    1944     858 
   Size:       1086    3833
 
BanI G'GyrC_C

Cuts at:   1935    2349    2597    2632    1935 
   Size:        414     248      35    4222

  Fragments arranged by size: 

                4222     414     248      35
 
BanII G_rGCy'C

Cuts at:    140     341     710     923    1007    1680    1933    2963     140 
   Size:        201     369     213      84     673     253    1030    2096

  Fragments arranged by size: 

                2096    1030     673     369     253     213     201      84
            
 
BbeI G_GCGC'C

Cuts at:   2353    2601    2353 
   Size:        248    4671
 
BbvCI CC'TCA_GC

Cuts at:    388    1130    1730     388 
   Size:        742     600    3577

  Fragments arranged by size: 

                3577     742     600
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:     90     124      90 
   Size:         34    4885
 
BciVI GTATCCnnnnn_n'

Cuts at:   1673    2817    3578    4919    1673 
   Size:       1144     761    1341    1673

  Fragments arranged by size: 

                1673    1341    1144     761
 
BclI T'GATC_A

Cuts at:   2439    2439 
   Size:       4919
 
BglII A'GATC_T

Cuts at:   2434    4471    2434 
   Size:       2037    2882
 
BlpI GC'TnA_GC

Cuts at:    313    1001    2031     313 
   Size:        688    1030    3201

  Fragments arranged by size: 

                3201    1030     688
 
Bme1580I G_kGCm'C

Cuts at:    341    2544    2637    3693     341 
   Size:       2203      93    1056    1567

  Fragments arranged by size: 

                2203    1567    1056      93
 
BmgBI CAC'GTC

Cuts at:   1842    1842 
   Size:       4919
 
BmrI ACTGGGnnnn_n'

Cuts at:    246    1653    2131    2212    2547    4691     246 
   Size:       1407     478      81     335    2144     474

  Fragments arranged by size: 

                2144    1407     478     474     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3286    3286 
   Size:       4919
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    721    1030    1180    1279    1882     721 
   Size:        309     150      99     603    3758

  Fragments arranged by size: 

                3758     603     309     150      99
 
Bpu10I CC'TnA_GC

Cuts at:    388    1130    1730     388 
   Size:        742     600    3577

  Fragments arranged by size: 

                3577     742     600
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:    790     865    1189    2095    2974    3466    3764     790 
   Size:         75     324     906     879     492     298    1945

  Fragments arranged by size: 

                1945     906     879     492     324     298      75
 
BsaI GGTCTCn'nnnn_

Cuts at:   1711    4623    1711 
   Size:       2912    2007
 
BsaAI yAC'GTr

Cuts at:   2272    2272 
   Size:       4919
 
BsaBI GATnn'nnATC

Cuts at:      6    2433    4470       6 
   Size:       2427    2037     455

  Fragments arranged by size: 

                2427    2037     455
 
BsaHI Gr'CG_yC

Cuts at:   1274    2350    2598    3300    4796    1274 
   Size:       1076     248     702    1496    1397

  Fragments arranged by size: 

                1496    1397    1076     702     248
 
BsaWI w'CCGG_w

Cuts at:    647    2108    2629    3307    3581    3728    4139     647 
   Size:       1461     521     678     274     147     411    1427

  Fragments arranged by size: 

                1461    1427     678     521     411     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:    442     472     818     848    1228    1258    1450    1480     442 
   Size:         30     346      30     380      30     192      30    3881

  Fragments arranged by size: 

                3881     380     346     192      30      30      30      30
            
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    223     363     466    1276    1651     223 
   Size:        140     103     810     375    3491

  Fragments arranged by size: 

                3491     810     375     140     103
 
BseYI C'CCAG_C

Cuts at:    215     227     233     239     283    2344    3102    3679    4175 
   Size:         12       6       6      44    2061     758     577     496

Cuts at:   4175     215 
   Size:        959

  Fragments arranged by size: 

                2061     959     758     577     496      44      12       6
                   6
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1436    1542    1436 
   Size:        106    4813
 
BsiEI CG_ry'CG

Cuts at:    104    2461    2507    3715     104 
   Size:       2357      46    1208    1308

  Fragments arranged by size: 

                2357    1308    1208      46
 
BsiHKAI G_wGCw'C

Cuts at:   1007    1064    1374    1933    2711    2901    3693    1007 
   Size:         57     310     559     778     190     792    2233

  Fragments arranged by size: 

                2233     792     778     559     310     190      57
 
BsmBI CGTCTCn'nnnn_

Cuts at:    970     970 
   Size:       4919
 
Bsp1286I G_dGCh'C

Cuts at:    140     341     710     923    1007    1064    1374    1680    1933 
   Size:        201     369     213      84      57     310     306     253

Cuts at:   1933    2544    2637    2711    2901    2963    3693     140 
   Size:        611      93      74     190      62     730    1366

  Fragments arranged by size: 

                1366     730     611     369     310     306     253     213
                 201     190      93      84      74      62      57
 
BspEI T'CCGG_A

Cuts at:    647    4139     647 
   Size:       3492    1427
 
BspHI T'CATG_A

Cuts at:   1400    4095    1400 
   Size:       2695    2224
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:   1969    2183    2485    2866    1969 
   Size:        214     302     381    4022

  Fragments arranged by size: 

                4022     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50     447     870    3211    3265    4749    4914      50 
   Size:        397     423    2341      54    1484     165      55

  Fragments arranged by size: 

                2341    1484     423     397     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   2831    2831 
   Size:       4919
 
BsrFI r'CCGG_y

Cuts at:   1185    1592    2917    3098    3307    1185 
   Size:        407    1325     181     209    2797

  Fragments arranged by size: 

                2797    1325     407     209     181
 
BsrGI T'GTAC_A

Cuts at:   1844    1844 
   Size:       4919
 
BssSI C'ACGA_G

Cuts at:    742    3190    3548     742 
   Size:       2448     358    2113

  Fragments arranged by size: 

                2448    2113     358
 
BstAPI GCAn_nnn'nTGC

Cuts at:   1369    2296    1369 
   Size:        927    3992
 
BstBI TT'CG_AA

Cuts at:   3280    3280 
   Size:       4919
 
BstYI r'GATC_y

Cuts at:      1     858    1013    1944    2411    2434    2769    3015    4027 
   Size:        857     155     931     467      23     335     246    1012

Cuts at:   4027    4113    4125    4471       1 
   Size:         86      12     346     449

  Fragments arranged by size: 

                1012     931     857     467     449     346     335     246
                 155      86      23      12
 
Bsu36I CC'TnA_GG

Cuts at:    614     614 
   Size:       4919
 
BtgI C'CryG_G

Cuts at:   1494    1494 
   Size:       4919
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    880    2869    3050    3213     880 
   Size:       1989     181     163    2586

  Fragments arranged by size: 

                2586    1989     181     163
 
BtsI GCAGTG_nn'

Cuts at:   1687    2162    1687 
   Size:        475    4444
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    171     206     171 
   Size:         35    4884
 
DraI TTT'AAA

Cuts at:   1918    1918 
   Size:       4919
 
DraIII CAC_nnn'GTG

Cuts at:    517     517 
   Size:       4919
 
DrdI GACnn_nn'nnGTC

Cuts at:   2625    3483    2625 
   Size:        858    4061
 
EaeI y'GGCC_r

Cuts at:    727    1328    2504    2678    3069    3096    4441     727 
   Size:        601    1176     174     391      27    1345    1205

  Fragments arranged by size: 

                1345    1205    1176     601     391     174      27
 
EagI C'GGCC_G

Cuts at:   2504    2504 
   Size:       4919
 
EarI CTCTTCn'nnn_

Cuts at:    649    1052    1321    1610    1744    1877    2942    3152     649 
   Size:        403     269     289     134     133    1065     210    2416

  Fragments arranged by size: 

                2416    1065     403     289     269     210     134     133
            
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   1309    1894    3127    3437    3583    4729    1309 
   Size:        585    1233     310     146    1146    1499

  Fragments arranged by size: 

                1499    1233    1146     585     310     146
 
EcoICRI GAG'CTC

Cuts at:   1005    1931    1005 
   Size:        926    3993
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    721    1030    1180    1279    1537    1768    1882    2744    3176 
   Size:        309     150      99     258     231     114     862     432

Cuts at:   3176    3923    4191     721 
   Size:        747     268    1449

  Fragments arranged by size: 

                1449     862     747     432     309     268     258     231
                 150     114      99
 
EcoNI CCTnn'n_nnAGG

Cuts at:   1099    1162    1099 
   Size:         63    4856
 
EcoO109I rG'GnC_Cy

Cuts at:    337     338    2058     337 
   Size:          1    1720    3198

  Fragments arranged by size: 

                3198    1720       1
 
EcoRI G'AATT_C

Cuts at:   1923    1923 
   Size:       4919
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   2938    2938 
   Size:       4919
 
FspI TGC'GCA

Cuts at:   2700    2700 
   Size:       4919
 
HaeII r_GCGC'y

Cuts at:   2353    2601    3623    4602    2353 
   Size:        248    1022     979    2670

  Fragments arranged by size: 

                2670    1022     979     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    487     519     969    1001    2761    2791    2793    2823     487 
   Size:         32     450      32    1760      30       2      30    2583

  Fragments arranged by size: 

                2583    1760     450      32      32      30      30       2
            
 
HincII GTy'rAC

Cuts at:   1958    1958 
   Size:       4919
 
HindIII A'AGCT_T

Cuts at:    412     412 
   Size:       4919
 
KasI G'GCGC_C

Cuts at:   2349    2597    2349 
   Size:        248    4671
 
KpnI G_GTAC'C

Cuts at:   1939    1939 
   Size:       4919
 
MluI A'CGCG_T

Cuts at:   4352    4352 
   Size:       4919
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:    296    1805    3590    3774     296 
   Size:       1509    1785     184    1441

  Fragments arranged by size: 

                1785    1509    1441     184
 
MscI TGG'CCA

Cuts at:    729    1330    2680     729 
   Size:        601    1350    2968

  Fragments arranged by size: 

                2968    1350     601
 
MslI CAynn'nnrTG

Cuts at:    584    2908    3035     584 
   Size:       2324     127    2468

  Fragments arranged by size: 

                2468    2324     127
 
MspA1I CmG'CkG

Cuts at:   1134    1326    1729    2030    2344    2704    3717    3962    1134 
   Size:        192     403     301     314     360    1013     245    2091

  Fragments arranged by size: 

                2091    1013     403     360     314     301     245     192
            
 
NaeI GCC'GGC

Cuts at:   3100    3100 
   Size:       4919
 
NarI GG'CG_CC

Cuts at:   2350    2598    2350 
   Size:        248    4671
 
NcoI C'CATG_G

Cuts at:   1494    1494 
   Size:       4919
 
NgoMIV G'CCGG_C

Cuts at:   3098    3098 
   Size:       4919
 
NspI r_CATG'y

Cuts at:   1972    2472    3379    1972 
   Size:        500     907    3512

  Fragments arranged by size: 

                3512     907     500
 
PciI A'CATG_T

Cuts at:   3375    3375 
   Size:       4919
 
PfoI T'CCnGG_A

Cuts at:   1007    1157    1007 
   Size:        150    4769
 
PmeI GTTT'AAAC

Cuts at:   1918    1918 
   Size:       4919
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   1228    1260    4058    4090    1228 
   Size:         32    2798      32    2057

  Fragments arranged by size: 

                2798    2057      32      32
 
PspOMI G'GGCC_C

Cuts at:    337     337 
   Size:       4919
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:   1378    1410    1654    1686    1378 
   Size:         32     244      32    4611

  Fragments arranged by size: 

                4611     244      32      32
 
PstI C_TGCA'G

Cuts at:    410    1966    2651     410 
   Size:       1556     685    2678

  Fragments arranged by size: 

                2678    1556     685
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       4919
 
PvuII CAG'CTG

Cuts at:   1134    1326    1729    2344    2704    1134 
   Size:        192     403     615     360    3349

  Fragments arranged by size: 

                3349     615     403     360     192
 
RsrII CG'GwC_CG

Cuts at:   3114    3114 
   Size:       4919
 
SacI G_AGCT'C

Cuts at:   1007    1933    1007 
   Size:        926    3993
 
SalI G'TCGA_C

Cuts at:   1956    1956 
   Size:       4919
 
SapI GCTCTTCn'nnn_

Cuts at:   1052    2942    3152    1052 
   Size:       1890     210    2819

  Fragments arranged by size: 

                2819    1890     210
 
SbfI CC_TGCA'GG

Cuts at:   1966    1966 
   Size:       4919
 
ScaI AGT'ACT

Cuts at:    328    4436     328 
   Size:       4108     811
 
SfcI C'TryA_G

Cuts at:     33     406     960    1458    1962    2647    3640    3831      33 
   Size:        373     554     498     504     685     993     191    1121

  Fragments arranged by size: 

                1121     993     685     554     504     498     373     191
            
 
SfoI GGC'GCC

Cuts at:   2351    2599    2351 
   Size:        248    4671
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4919
 
SmaI CCC'GGG

Cuts at:   1941    1941 
   Size:       4919
 
SmlI C'TyrA_G

Cuts at:    769     863     880    1204    2074    2989    3481    3743     769 
   Size:         94      17     324     870     915     492     262    1945

  Fragments arranged by size: 

                1945     915     870     492     324     262      94      17
            
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       4919
 
SphI G_CATG'C

Cuts at:   1972    2472    1972 
   Size:        500    4419
 
StyI C'CwwG_G

Cuts at:    143    1232    1494    2053    2407     143 
   Size:       1089     262     559     354    2655

  Fragments arranged by size: 

                2655    1089     559     354     262
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    592    2502    2635    3094    3303     592 
   Size:       1910     133     459     209    2208

  Fragments arranged by size: 

                2208    1910     459     209     133
 
TatI w'GTAC_w

Cuts at:    326     569     806     825    1394    1700    1844    4434     326 
   Size:        243     237      19     569     306     144    2590     811

  Fragments arranged by size: 

                2590     811     569     306     243     237     144      19
            
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   1606    1638    4436    4468    1606 
   Size:         32    2798      32    2057

  Fragments arranged by size: 

                2798    2057      32      32
 
Tth111I GACn'n_nGTC

Cuts at:   2716    2716 
   Size:       4919
 
XbaI T'CTAG_A

Cuts at:   1950    1950 
   Size:       4919
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   1857    2414    1857 
   Size:        557    4362
 
XhoI C'TCGA_G

Cuts at:    863     863 
   Size:       4919
 
XmaI C'CCGG_G

Cuts at:   1939    1939 
   Size:       4919
 
XmnI GAAnn'nnTTC

Cuts at:    643     643 
   Size:       4919
 
ZraI GAC'GTC

Cuts at:   1275    1275 
   Size:       4919
 

 Enzymes that do cut:

    AatII     AccI   Acc65I     AclI     AcuI   AflIII     AgeI     AhdI
     AloI    AlwNI     ApaI    ApaLI     ApoI     AseI    AsiSI     AvaI
    BamHI     BanI    BanII     BbeI    BbvCI     BcgI    BciVI     BclI
    BglII     BlpI Bme1580I    BmgBI     BmrI     BplI     BpmI   Bpu10I
    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI
    BseYI     BsgI    BsiEI  BsiHKAI    BsmBI Bsp1286I    BspEI    BspHI
    BspMI    BsrBI    BsrDI    BsrFI    BsrGI    BssSI   BstAPI    BstBI
    BstYI   Bsu36I     BtgI    BtgZI     BtsI    CspCI     DraI   DraIII
     DrdI     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI    EcoNI
 EcoO109I    EcoRI     FalI     FspI    HaeII    Hin4I   HincII  HindIII
     KasI     KpnI     MluI     MmeI     MscI     MslI   MspA1I     NaeI
     NarI     NcoI   NgoMIV     NspI     PciI     PfoI     PmeI     PpiI
   PspOMI     PsrI     PstI     PvuI    PvuII    RsrII     SacI     SalI
     SapI     SbfI     ScaI     SfcI     SfoI     SgfI     SmaI     SmlI
     SpeI     SphI     StyI    TaqII     TatI     TstI  Tth111I     XbaI
     XcmI     XhoI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AarI     AfeI    AflII     AleI     AlfI     AscI    AvrII     BaeI
     BbsI    BfrBI     BglI     BmtI    BsiWI     BsmI   BssHII   BstEII
    BstXI  BstZ17I     ClaI    EcoRV     FseI    FspAI     HpaI     MfeI
     NdeI     NheI     NotI     NruI     NsiI     PacI     PasI    PflMI
     PmlI    PpuMI    PshAI     PsiI    PspXI    SacII    SanDI    SexAI
     SfiI    SgrAI    SnaBI     SrfI     SspI     StuI     SwaI
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