Restriction map for
pF1KB6155
(Circular) (Six-base) MAPSORT of: pF1KB6155.seq Check: 9524 from: 1 to: 4904
pF1KB6155 4904 bp
With 182 enzymes: SgfI *
June 26, 2008 10:46 ..
AatII G_ACGT'C
Cuts at: 1784 1784
Size: 4904
AccI GT'mk_AC
Cuts at: 1942 1942
Size: 4904
Acc65I G'GTAC_C
Cuts at: 1920 1920
Size: 4904
AclI AA'CG_TT
Cuts at: 4744 4744
Size: 4904
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2729 3161 3908 4176 2729
Size: 432 747 268 3457
Fragments arranged by size:
3457 747 432 268
AflIII A'CryG_T
Cuts at: 2256 3360 4337 2256
Size: 1104 977 2823
Fragments arranged by size:
2823 1104 977
AgeI A'CCGG_T
Cuts at: 3292 3292
Size: 4904
AleI CACnn'nnGTG
Cuts at: 1462 1462
Size: 4904
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1214 1214
Size: 4904
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 340 372 340
Size: 32 4872
AlwNI CAG_nnn'CTG
Cuts at: 126 225 903 1611 2209 3776 126
Size: 99 678 708 598 1567 1254
Fragments arranged by size:
1567 1254 708 678 598 99
ApaI G_GGCC'C
Cuts at: 521 521
Size: 4904
ApaLI G'TGCA_C
Cuts at: 3674 3674
Size: 4904
ApoI r'AATT_y
Cuts at: 1908 4257 4513 1908
Size: 2349 256 2299
Fragments arranged by size:
2349 2299 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4904
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4904
AvaI C'yCGr_G
Cuts at: 397 1652 1924 397
Size: 1255 272 3377
Fragments arranged by size:
3377 1255 272
BamHI G'GATC_C
Cuts at: 1929 1929
Size: 4904
BanI G'GyrC_C
Cuts at: 194 657 995 1407 1920 2334 2582 2617 194
Size: 463 338 412 513 414 248 35 2481
Fragments arranged by size:
2481 513 463 414 412 338 248 35
BanII G_rGCy'C
Cuts at: 118 263 521 673 713 820 1133 1346 1918
Size: 145 258 152 40 107 313 213 572
Cuts at: 1918 2948 118
Size: 1030 2074
Fragments arranged by size:
2074 1030 572 313 258 213 152 145
107 40
BbeI G_GCGC'C
Cuts at: 198 2338 2586 198
Size: 2140 248 2516
Fragments arranged by size:
2516 2140 248
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4870
BciVI GTATCCnnnnn_n'
Cuts at: 2802 3563 4904 2802
Size: 761 1341 2802
Fragments arranged by size:
2802 1341 761
BclI T'GATC_A
Cuts at: 1466 2424 1466
Size: 958 3946
BglI GCCn_nnn'nGGC
Cuts at: 149 149
Size: 4904
BglII A'GATC_T
Cuts at: 2419 4456 2419
Size: 2037 2867
BlpI GC'TnA_GC
Cuts at: 2016 2016
Size: 4904
Bme1580I G_kGCm'C
Cuts at: 521 1412 2529 2622 3678 521
Size: 891 1117 93 1056 1747
Fragments arranged by size:
1747 1117 1056 891 93
BmgBI CAC'GTC
Cuts at: 1892 1892
Size: 4904
BmrI ACTGGGnnnn_n'
Cuts at: 187 1496 1599 2116 2197 2532 4676 187
Size: 1309 103 517 81 335 2144 415
Fragments arranged by size:
2144 1309 517 415 335 103 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3271 3271
Size: 4904
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 348 1102 1120 1189 1633 1846 348
Size: 754 18 69 444 213 3406
Fragments arranged by size:
3406 754 444 213 69 18
Bpu10I CC'TnA_GC
Cuts at: 158 158
Size: 4904
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1321 2080 2959 3451 3749 1321
Size: 759 879 492 298 2476
Fragments arranged by size:
2476 879 759 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 293 4608 293
Size: 4315 589
BsaAI yAC'GTr
Cuts at: 2257 2257
Size: 4904
BsaBI GATnn'nnATC
Cuts at: 6 1801 2418 4455 6
Size: 1795 617 2037 455
Fragments arranged by size:
2037 1795 617 455
BsaHI Gr'CG_yC
Cuts at: 195 1781 2335 2583 3285 4781 195
Size: 1586 554 248 702 1496 318
Fragments arranged by size:
1586 1496 702 554 318 248
BsaWI w'CCGG_w
Cuts at: 341 2093 2614 3292 3566 3713 4124 341
Size: 1752 521 678 274 147 411 1121
Fragments arranged by size:
1752 1121 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 340 370 700 730 1585 1615 1687 1717 340
Size: 30 330 30 855 30 72 30 3527
Fragments arranged by size:
3527 855 330 72 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 396 948 1141 1260 1353 1828 396
Size: 552 193 119 93 475 3472
Fragments arranged by size:
3472 552 475 193 119 93
BseYI C'CCAG_C
Cuts at: 513 661 903 977 1698 2329 3087 3664 4160
Size: 148 242 74 721 631 758 577 496
Cuts at: 4160 513
Size: 1257
Fragments arranged by size:
1257 758 721 631 577 496 242 148
74
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 238 427 1339 1699 238
Size: 189 912 360 3443
Fragments arranged by size:
3443 912 360 189
BsiEI CG_ry'CG
Cuts at: 104 1249 2446 2492 3700 104
Size: 1145 1197 46 1208 1308
Fragments arranged by size:
1308 1208 1197 1145 46
BsiHKAI G_wGCw'C
Cuts at: 134 1133 1178 1918 2696 2886 3678 134
Size: 999 45 740 778 190 792 1360
Fragments arranged by size:
1360 999 792 778 740 190 45
BsmBI CGTCTCn'nnnn_
Cuts at: 1788 1882 1788
Size: 94 4810
Bsp1286I G_dGCh'C
Cuts at: 118 134 263 521 673 713 820 1133 1178
Size: 16 129 258 152 40 107 313 45
Cuts at: 1178 1346 1412 1918 2529 2622 2696 2886 2948
Size: 168 66 506 611 93 74 190 62
Cuts at: 2948 3678 118
Size: 730 1344
Fragments arranged by size:
1344 730 611 506 313 258 190 168
152 129 107 93 74 66 62 45
40 16
BspEI T'CCGG_A
Cuts at: 341 4124 341
Size: 3783 1121
BspHI T'CATG_A
Cuts at: 4080 4080
Size: 4904
BspMI ACCTGCnnnn'nnnn_
Cuts at: 359 1954 2168 2470 2851 359
Size: 1595 214 302 381 2412
Fragments arranged by size:
2412 1595 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1040 1086 3196 3250 4734 4899 50
Size: 990 46 2110 54 1484 165 55
Fragments arranged by size:
2110 1484 990 165 55 54 46
BsrDI GCAATG_nn'
Cuts at: 2816 2816
Size: 4904
BsrFI r'CCGG_y
Cuts at: 197 766 1148 1685 2902 3083 3292 197
Size: 569 382 537 1217 181 209 1809
Fragments arranged by size:
1809 1217 569 537 382 209 181
BssSI C'ACGA_G
Cuts at: 966 1000 1306 3175 3533 966
Size: 34 306 1869 358 2337
Fragments arranged by size:
2337 1869 358 306 34
BstAPI GCAn_nnn'nTGC
Cuts at: 2281 2281
Size: 4904
BstBI TT'CG_AA
Cuts at: 1049 3265 1049
Size: 2216 2688
BstYI r'GATC_y
Cuts at: 1 602 1301 1929 2396 2419 2754 3000 4012
Size: 601 699 628 467 23 335 246 1012
Cuts at: 4012 4098 4110 4456 1
Size: 86 12 346 449
Fragments arranged by size:
1012 699 628 601 467 449 346 335
246 86 23 12
BtgI C'CryG_G
Cuts at: 940 940
Size: 4904
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 512 1005 1248 2854 3035 3198 512
Size: 493 243 1606 181 163 2218
Fragments arranged by size:
2218 1606 493 243 181 163
BtsI GCAGTG_nn'
Cuts at: 359 2147 359
Size: 1788 3116
DraI TTT'AAA
Cuts at: 1903 1903
Size: 4904
DraIII CAC_nnn'GTG
Cuts at: 1002 1002
Size: 4904
DrdI GACnn_nn'nnGTC
Cuts at: 385 2610 3468 385
Size: 2225 858 1821
Fragments arranged by size:
2225 1821 858
EaeI y'GGCC_r
Cuts at: 431 1246 2489 2663 3054 3081 4426 431
Size: 815 1243 174 391 27 1345 909
Fragments arranged by size:
1345 1243 909 815 391 174 27
EagI C'GGCC_G
Cuts at: 1246 2489 1246
Size: 1243 3661
EarI CTCTTCn'nnn_
Cuts at: 139 931 2927 3137 139
Size: 792 1996 210 1906
Fragments arranged by size:
1996 1906 792 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3112 3422 3568 4714 3112
Size: 310 146 1146 3302
Fragments arranged by size:
3302 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1131 1916 1131
Size: 785 4119
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 348 1102 1120 1189 1633 1846 2729 3161 3908
Size: 754 18 69 444 213 883 432 747
Cuts at: 3908 4176 348
Size: 268 1076
Fragments arranged by size:
1076 883 754 747 444 432 268 213
69 18
EcoNI CCTnn'n_nnAGG
Cuts at: 652 1391 652
Size: 739 4165
EcoO109I rG'GnC_Cy
Cuts at: 222 517 1253 1353 1396 1477 2043 222
Size: 295 736 100 43 81 566 3083
Fragments arranged by size:
3083 736 566 295 100 81 43
EcoRI G'AATT_C
Cuts at: 1908 1908
Size: 4904
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 441 2923 441
Size: 2482 2422
FspI TGC'GCA
Cuts at: 2685 2685
Size: 4904
HaeII r_GCGC'y
Cuts at: 198 476 1878 2338 2586 3608 4587 198
Size: 278 1402 460 248 1022 979 515
Fragments arranged by size:
1402 1022 979 515 460 278 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 165 197 700 732 943 975 1496 1528 1687
Size: 32 503 32 211 32 521 32 159
Cuts at: 1687 1719 2746 2776 2778 2808 165
Size: 32 1027 30 2 30 2261
Fragments arranged by size:
2261 1027 521 503 211 159 32 32
32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1943 1943
Size: 4904
KasI G'GCGC_C
Cuts at: 194 2334 2582 194
Size: 2140 248 2516
Fragments arranged by size:
2516 2140 248
KpnI G_GTAC'C
Cuts at: 1924 1924
Size: 4904
MluI A'CGCG_T
Cuts at: 4337 4337
Size: 4904
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 149 776 890 1205 3575 3759 149
Size: 627 114 315 2370 184 1294
Fragments arranged by size:
2370 1294 627 315 184 114
MscI TGG'CCA
Cuts at: 433 2665 433
Size: 2232 2672
MslI CAynn'nnrTG
Cuts at: 1462 2893 3020 1462
Size: 1431 127 3346
Fragments arranged by size:
3346 1431 127
MspA1I CmG'CkG
Cuts at: 684 888 942 1198 1568 1777 2015 2329 2689
Size: 204 54 256 370 209 238 314 360
Cuts at: 2689 3702 3947 684
Size: 1013 245 1641
Fragments arranged by size:
1641 1013 370 360 314 256 245 238
209 204 54
NaeI GCC'GGC
Cuts at: 199 3085 199
Size: 2886 2018
NarI GG'CG_CC
Cuts at: 195 2335 2583 195
Size: 2140 248 2516
Fragments arranged by size:
2516 2140 248
NgoMIV G'CCGG_C
Cuts at: 197 3083 197
Size: 2886 2018
NruI TCG'CGA
Cuts at: 185 185
Size: 4904
NspI r_CATG'y
Cuts at: 1957 2457 3364 1957
Size: 500 907 3497
Fragments arranged by size:
3497 907 500
PasI CC'CwG_GG
Cuts at: 1387 1530 1387
Size: 143 4761
PciI A'CATG_T
Cuts at: 3360 3360
Size: 4904
PflMI CCAn_nnn'nTGG
Cuts at: 903 1014 1080 903
Size: 111 66 4727
Fragments arranged by size:
4727 111 66
PfoI T'CCnGG_A
Cuts at: 609 782 1166 1514 1691 1808 609
Size: 173 384 348 177 117 3705
Fragments arranged by size:
3705 384 348 177 173 117
PmeI GTTT'AAAC
Cuts at: 1903 1903
Size: 4904
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 340 372 4043 4075 340
Size: 32 3671 32 1169
Fragments arranged by size:
3671 1169 32 32
PpuMI rG'GwC_Cy
Cuts at: 1396 1396
Size: 4904
PspOMI G'GGCC_C
Cuts at: 517 517
Size: 4904
PstI C_TGCA'G
Cuts at: 461 938 1301 1831 1951 2636 461
Size: 477 363 530 120 685 2729
Fragments arranged by size:
2729 685 530 477 363 120
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4904
PvuII CAG'CTG
Cuts at: 1198 2329 2689 1198
Size: 1131 360 3413
Fragments arranged by size:
3413 1131 360
RsrII CG'GwC_CG
Cuts at: 3099 3099
Size: 4904
SacI G_AGCT'C
Cuts at: 1133 1918 1133
Size: 785 4119
SacII CC_GC'GG
Cuts at: 943 943
Size: 4904
SalI G'TCGA_C
Cuts at: 1941 1941
Size: 4904
SapI GCTCTTCn'nnn_
Cuts at: 139 931 2927 3137 139
Size: 792 1996 210 1906
Fragments arranged by size:
1996 1906 792 210
SbfI CC_TGCA'GG
Cuts at: 1951 1951
Size: 4904
ScaI AGT'ACT
Cuts at: 4421 4421
Size: 4904
SfcI C'TryA_G
Cuts at: 33 287 457 934 1297 1313 1827 1947 2632
Size: 254 170 477 363 16 514 120 685
Cuts at: 2632 3625 3816 33
Size: 993 191 1121
Fragments arranged by size:
1121 993 685 514 477 363 254 191
170 120 16
SfoI GGC'GCC
Cuts at: 196 2336 2584 196
Size: 2140 248 2516
Fragments arranged by size:
2516 2140 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4904
SgrAI Cr'CCGG_yG
Cuts at: 197 197
Size: 4904
SmaI CCC'GGG
Cuts at: 1654 1926 1654
Size: 272 4632
SmlI C'TyrA_G
Cuts at: 1336 2059 2974 3466 3728 1336
Size: 723 915 492 262 2512
Fragments arranged by size:
2512 915 723 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4904
SphI G_CATG'C
Cuts at: 1957 2457 1957
Size: 500 4404
StyI C'CwwG_G
Cuts at: 294 1116 2038 2392 294
Size: 822 922 354 2806
Fragments arranged by size:
2806 922 822 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 675 756 1407 2487 2620 3079 3288 675
Size: 81 651 1080 133 459 209 2291
Fragments arranged by size:
2291 1080 651 459 209 133 81
TatI w'GTAC_w
Cuts at: 4419 4419
Size: 4904
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 988 1020 1585 1617 1862 1894 4421 4453 988
Size: 32 565 32 245 32 2527 32 1439
Fragments arranged by size:
2527 1439 565 245 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 179 2701 179
Size: 2522 2382
XbaI T'CTAG_A
Cuts at: 1935 1935
Size: 4904
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1537 1816 2399 1537
Size: 279 583 4042
Fragments arranged by size:
4042 583 279
XmaI C'CCGG_G
Cuts at: 1652 1924 1652
Size: 272 4632
XmnI GAAnn'nnTTC
Cuts at: 322 730 322
Size: 408 4496
ZraI GAC'GTC
Cuts at: 1782 1782
Size: 4904
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AgeI AleI
AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstYI BtgI BtgZI BtsI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NgoMIV NruI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PspOMI PstI PvuI PvuII RsrII SacI SacII SalI
SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI
SmlI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AflII AhdI AscI AvrII BaeI BbsI
BbvCI BfrBI BmtI BsiWI BsmI BsrGI BssHII BstEII
BstXI BstZ17I Bsu36I ClaI CspCI EcoRV FseI FspAI
HindIII HpaI MfeI NcoI NdeI NheI NotI NsiI
PacI PmlI PshAI PsiI PspXI PsrI SanDI SexAI
SfiI SnaBI SrfI SspI StuI SwaI XhoI