(Circular) (Six-base) MAPSORT of: pF1KB6143.seq Check: 379 from: 1 to: 4898 pF1KB6143 4898 bp With 182 enzymes: SgfI * October 7, 2009 14:19 .. AccI GT'mk_AC Cuts at: 1936 1936 Size: 4898 Acc65I G'GTAC_C Cuts at: 1914 1914 Size: 4898 AclI AA'CG_TT Cuts at: 4738 4738 Size: 4898 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1084 2723 3155 3902 4170 1084 Size: 1639 432 747 268 1812 Fragments arranged by size: 1812 1639 747 432 268 AfeI AGC'GCT Cuts at: 1384 1384 Size: 4898 AflIII A'CryG_T Cuts at: 1745 2250 3354 4331 1745 Size: 505 1104 977 2312 Fragments arranged by size: 2312 1104 977 505 AgeI A'CCGG_T Cuts at: 3286 3286 Size: 4898 AhdI GACnn_n'nnGTC Cuts at: 429 429 Size: 4898 AlwNI CAG_nnn'CTG Cuts at: 331 583 2203 3770 331 Size: 252 1620 1567 1459 Fragments arranged by size: 1620 1567 1459 252 ApaI G_GGCC'C Cuts at: 489 623 1396 489 Size: 134 773 3991 Fragments arranged by size: 3991 773 134 ApaLI G'TGCA_C Cuts at: 3668 3668 Size: 4898 ApoI r'AATT_y Cuts at: 281 321 713 727 800 1072 1170 1902 4251 Size: 40 392 14 73 272 98 732 2349 Cuts at: 4251 4507 281 Size: 256 672 Fragments arranged by size: 2349 732 672 392 272 256 98 73 40 14 AseI AT'TA_AT Cuts at: 20 20 Size: 4898 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 4898 AvaI C'yCGr_G Cuts at: 122 1918 122 Size: 1796 3102 BamHI G'GATC_C Cuts at: 1923 1923 Size: 4898 BanI G'GyrC_C Cuts at: 1266 1914 2328 2576 2611 1266 Size: 648 414 248 35 3553 Fragments arranged by size: 3553 648 414 248 35 BanII G_rGCy'C Cuts at: 489 510 623 1396 1912 2942 489 Size: 21 113 773 516 1030 2445 Fragments arranged by size: 2445 1030 773 516 113 21 BbeI G_GCGC'C Cuts at: 2332 2580 2332 Size: 248 4650 BbsI GAAGACnn'nnnn_ Cuts at: 614 614 Size: 4898 BbvCI CC'TCA_GC Cuts at: 1861 1873 1861 Size: 12 4886 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1831 1865 1831 Size: 34 4864 BciVI GTATCCnnnnn_n' Cuts at: 2796 3557 4898 2796 Size: 761 1341 2796 Fragments arranged by size: 2796 1341 761 BclI T'GATC_A Cuts at: 2418 2418 Size: 4898 BglI GCCn_nnn'nGGC Cuts at: 118 118 Size: 4898 BglII A'GATC_T Cuts at: 1482 1560 2413 4450 1482 Size: 78 853 2037 1930 Fragments arranged by size: 2037 1930 853 78 BlpI GC'TnA_GC Cuts at: 674 2010 674 Size: 1336 3562 Bme1580I G_kGCm'C Cuts at: 489 623 1396 2523 2616 3672 489 Size: 134 773 1127 93 1056 1715 Fragments arranged by size: 1715 1127 1056 773 134 93 BmrI ACTGGGnnnn_n' Cuts at: 284 471 546 609 621 2110 2191 2526 4670 Size: 187 75 63 12 1489 81 335 2144 Cuts at: 4670 284 Size: 512 Fragments arranged by size: 2144 1489 512 335 187 81 75 63 12 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3265 3265 Size: 4898 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 459 524 1840 459 Size: 65 1316 3517 Fragments arranged by size: 3517 1316 65 Bpu10I CC'TnA_GC Cuts at: 371 1861 1873 371 Size: 1490 12 3396 Fragments arranged by size: 3396 1490 12 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2074 2953 3445 3743 2074 Size: 879 492 298 3229 Fragments arranged by size: 3229 879 492 298 BsaI GGTCTCn'nnnn_ Cuts at: 4602 4602 Size: 4898 BsaAI yAC'GTr Cuts at: 2251 2251 Size: 4898 BsaBI GATnn'nnATC Cuts at: 6 2412 4449 6 Size: 2406 2037 455 Fragments arranged by size: 2406 2037 455 BsaHI Gr'CG_yC Cuts at: 2329 2577 3279 4775 2329 Size: 248 702 1496 2452 Fragments arranged by size: 2452 1496 702 248 BsaWI w'CCGG_w Cuts at: 204 1781 2087 2608 3286 3560 3707 4118 204 Size: 1577 306 521 678 274 147 411 984 Fragments arranged by size: 1577 984 678 521 411 306 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 510 540 510 Size: 30 4868 BseYI C'CCAG_C Cuts at: 479 488 2323 3081 3658 4154 479 Size: 9 1835 758 577 496 1223 Fragments arranged by size: 1835 1223 758 577 496 9 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1371 1371 Size: 4898 BsiEI CG_ry'CG Cuts at: 104 2440 2486 3694 104 Size: 2336 46 1208 1308 Fragments arranged by size: 2336 1308 1208 46 BsiHKAI G_wGCw'C Cuts at: 1376 1912 2690 2880 3672 1376 Size: 536 778 190 792 2602 Fragments arranged by size: 2602 792 778 536 190 BsmBI CGTCTCn'nnnn_ Cuts at: 1000 1000 Size: 4898 Bsp1286I G_dGCh'C Cuts at: 489 510 623 1376 1396 1912 2523 2616 2690 Size: 21 113 753 20 516 611 93 74 Cuts at: 2690 2880 2942 3672 489 Size: 190 62 730 1715 Fragments arranged by size: 1715 753 730 611 516 190 113 93 74 62 21 20 BspEI T'CCGG_A Cuts at: 204 4118 204 Size: 3914 984 BspHI T'CATG_A Cuts at: 1238 4074 1238 Size: 2836 2062 BspMI ACCTGCnnnn'nnnn_ Cuts at: 1948 2162 2464 2845 1948 Size: 214 302 381 4001 Fragments arranged by size: 4001 381 302 214 BsrBI CCG'CTC Cuts at: 50 3190 3244 4728 4893 50 Size: 3140 54 1484 165 55 Fragments arranged by size: 3140 1484 165 55 54 BsrDI GCAATG_nn' Cuts at: 426 2810 426 Size: 2384 2514 BsrFI r'CCGG_y Cuts at: 2896 3077 3286 2896 Size: 181 209 4508 Fragments arranged by size: 4508 209 181 BssSI C'ACGA_G Cuts at: 1537 1588 3169 3527 1537 Size: 51 1581 358 2908 Fragments arranged by size: 2908 1581 358 51 BstAPI GCAn_nnn'nTGC Cuts at: 1830 2275 1830 Size: 445 4453 BstBI TT'CG_AA Cuts at: 3259 3259 Size: 4898 BstXI CCAn_nnnn'nTGG Cuts at: 1332 1539 1332 Size: 207 4691 BstYI r'GATC_y Cuts at: 1 873 1482 1560 1784 1923 2390 2413 2748 Size: 872 609 78 224 139 467 23 335 Cuts at: 2748 2994 4006 4092 4104 4450 1 Size: 246 1012 86 12 346 449 Fragments arranged by size: 1012 872 609 467 449 346 335 246 224 139 86 78 23 12 BtgI C'CryG_G Cuts at: 107 1253 1331 107 Size: 1146 78 3674 Fragments arranged by size: 3674 1146 78 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 2848 3029 3192 2848 Size: 181 163 4554 Fragments arranged by size: 4554 181 163 BtsI GCAGTG_nn' Cuts at: 420 542 2141 420 Size: 122 1599 3177 Fragments arranged by size: 3177 1599 122 ClaI AT'CG_AT Cuts at: 948 948 Size: 4898 DraI TTT'AAA Cuts at: 1897 1897 Size: 4898 DrdI GACnn_nn'nnGTC Cuts at: 1048 2604 3462 1048 Size: 1556 858 2484 Fragments arranged by size: 2484 1556 858 EaeI y'GGCC_r Cuts at: 110 552 770 1442 2483 2657 3048 3075 4420 Size: 442 218 672 1041 174 391 27 1345 Cuts at: 4420 110 Size: 588 Fragments arranged by size: 1345 1041 672 588 442 391 218 174 27 EagI C'GGCC_G Cuts at: 2483 2483 Size: 4898 EarI CTCTTCn'nnn_ Cuts at: 636 1040 1061 1717 2921 3131 636 Size: 404 21 656 1204 210 2403 Fragments arranged by size: 2403 1204 656 404 210 21 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 1798 3106 3416 3562 4708 1798 Size: 1308 310 146 1146 1988 Fragments arranged by size: 1988 1308 1146 310 146 EcoICRI GAG'CTC Cuts at: 1910 1910 Size: 4898 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 459 524 1084 1840 2723 3155 3902 4170 459 Size: 65 560 756 883 432 747 268 1187 Fragments arranged by size: 1187 883 756 747 560 432 268 65 EcoO109I rG'GnC_Cy Cuts at: 485 1869 2037 485 Size: 1384 168 3346 Fragments arranged by size: 3346 1384 168 EcoRI G'AATT_C Cuts at: 1902 1902 Size: 4898 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 361 1621 2917 361 Size: 1260 1296 2342 Fragments arranged by size: 2342 1296 1260 FspI TGC'GCA Cuts at: 1087 2679 1087 Size: 1592 3306 FspAI rTGC'GCAy Cuts at: 1087 1087 Size: 4898 HaeII r_GCGC'y Cuts at: 674 1386 2332 2580 3602 4581 674 Size: 712 946 248 1022 979 991 Fragments arranged by size: 1022 991 979 946 712 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 173 205 340 372 415 447 637 669 1718 Size: 32 135 32 43 32 190 32 1049 Cuts at: 1718 1750 2740 2770 2772 2802 173 Size: 32 990 30 2 30 2269 Fragments arranged by size: 2269 1049 990 190 135 43 32 32 32 32 32 30 30 2 HincII GTy'rAC Cuts at: 1937 1937 Size: 4898 KasI G'GCGC_C Cuts at: 2328 2576 2328 Size: 248 4650 KpnI G_GTAC'C Cuts at: 1918 1918 Size: 4898 MluI A'CGCG_T Cuts at: 1745 4331 1745 Size: 2586 2312 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 795 3569 3753 795 Size: 2774 184 1940 Fragments arranged by size: 2774 1940 184 MscI TGG'CCA Cuts at: 112 772 1444 2659 112 Size: 660 672 1215 2351 Fragments arranged by size: 2351 1215 672 660 MslI CAynn'nnrTG Cuts at: 2887 3014 2887 Size: 127 4771 MspA1I CmG'CkG Cuts at: 658 2009 2323 2683 3696 3941 658 Size: 1351 314 360 1013 245 1615 Fragments arranged by size: 1615 1351 1013 360 314 245 NaeI GCC'GGC Cuts at: 3079 3079 Size: 4898 NarI GG'CG_CC Cuts at: 2329 2577 2329 Size: 248 4650 NcoI C'CATG_G Cuts at: 107 1253 1331 107 Size: 1146 78 3674 Fragments arranged by size: 3674 1146 78 NgoMIV G'CCGG_C Cuts at: 3077 3077 Size: 4898 NspI r_CATG'y Cuts at: 1951 2451 3358 1951 Size: 500 907 3491 Fragments arranged by size: 3491 907 500 PciI A'CATG_T Cuts at: 3354 3354 Size: 4898 PflMI CCAn_nnn'nTGG Cuts at: 536 583 536 Size: 47 4851 PfoI T'CCnGG_A Cuts at: 732 732 Size: 4898 PmeI GTTT'AAAC Cuts at: 1897 1897 Size: 4898 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 245 277 4037 4069 245 Size: 32 3760 32 1074 Fragments arranged by size: 3760 1074 32 32 PspOMI G'GGCC_C Cuts at: 485 619 1392 485 Size: 134 773 3991 Fragments arranged by size: 3991 773 134 PstI C_TGCA'G Cuts at: 1945 2630 1945 Size: 685 4213 PvuI CG_AT'CG Cuts at: 104 104 Size: 4898 PvuII CAG'CTG Cuts at: 658 2323 2683 658 Size: 1665 360 2873 Fragments arranged by size: 2873 1665 360 RsrII CG'GwC_CG Cuts at: 3093 3093 Size: 4898 SacI G_AGCT'C Cuts at: 1912 1912 Size: 4898 SalI G'TCGA_C Cuts at: 1935 1935 Size: 4898 SapI GCTCTTCn'nnn_ Cuts at: 2921 3131 2921 Size: 210 4688 SbfI CC_TGCA'GG Cuts at: 1945 1945 Size: 4898 ScaI AGT'ACT Cuts at: 4415 4415 Size: 4898 SexAI A'CCwGG_T Cuts at: 404 404 Size: 4898 SfcI C'TryA_G Cuts at: 33 1941 2626 3619 3810 33 Size: 1908 685 993 191 1121 Fragments arranged by size: 1908 1121 993 685 191 SfoI GGC'GCC Cuts at: 2330 2578 2330 Size: 248 4650 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 4898 SmaI CCC'GGG Cuts at: 1920 1920 Size: 4898 SmlI C'TyrA_G Cuts at: 2053 2968 3460 3722 2053 Size: 915 492 262 3229 Fragments arranged by size: 3229 915 492 262 SpeI A'CTAG_T Cuts at: 67 1674 67 Size: 1607 3291 SphI G_CATG'C Cuts at: 1951 2451 1951 Size: 500 4398 StuI AGG'CCT Cuts at: 565 1105 1429 565 Size: 540 324 4034 Fragments arranged by size: 4034 540 324 StyI C'CwwG_G Cuts at: 107 116 570 1253 1325 1331 1403 2032 2386 Size: 9 454 683 72 6 72 629 354 Cuts at: 2386 107 Size: 2619 Fragments arranged by size: 2619 683 629 454 354 72 72 9 6 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 2481 2614 3073 3282 2481 Size: 133 459 209 4097 Fragments arranged by size: 4097 459 209 133 TatI w'GTAC_w Cuts at: 4413 4413 Size: 4898 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 311 343 510 542 4415 4447 311 Size: 32 167 32 3873 32 762 Fragments arranged by size: 3873 762 167 32 32 32 Tth111I GACn'n_nGTC Cuts at: 2695 2695 Size: 4898 XbaI T'CTAG_A Cuts at: 1929 1929 Size: 4898 XcmI CCAnnnn_n'nnnnTGG Cuts at: 412 2393 412 Size: 1981 2917 XmaI C'CCGG_G Cuts at: 1918 1918 Size: 4898 Enzymes that do cut: AccI Acc65I AclI AcuI AfeI AflIII AgeI AhdI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI ClaI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI FspAI HaeII Hin4I HincII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI PflMI PfoI PmeI PpiI PspOMI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI SpeI SphI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI Enzymes that do not cut: AarI AatII AflII AleI AlfI AloI AscI AvrII BaeI BfrBI BmgBI BmtI BseRI BsiWI BsmI BsrGI BssHII BstEII BstZ17I Bsu36I CspCI DraIII EcoNI EcoRV FseI HindIII HpaI MfeI NdeI NheI NotI NruI NsiI PacI PasI PmlI PpuMI PshAI PsiI PspXI PsrI SacII SanDI SfiI SgrAI SnaBI SrfI SspI SwaI XhoI XmnI ZraI