Restriction map for pF1KB6143
 (Circular) (Six-base) MAPSORT of: pF1KB6143.seq  Check: 379  from: 1  to: 4898



pF1KB6143  4898 bp

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:19  ..

AccI GT'mk_AC

Cuts at:   1936    1936 
   Size:       4898
 
Acc65I G'GTAC_C

Cuts at:   1914    1914 
   Size:       4898
 
AclI AA'CG_TT

Cuts at:   4738    4738 
   Size:       4898
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1084    2723    3155    3902    4170    1084 
   Size:       1639     432     747     268    1812

  Fragments arranged by size: 

                1812    1639     747     432     268
 
AfeI AGC'GCT

Cuts at:   1384    1384 
   Size:       4898
 
AflIII A'CryG_T

Cuts at:   1745    2250    3354    4331    1745 
   Size:        505    1104     977    2312

  Fragments arranged by size: 

                2312    1104     977     505
 
AgeI A'CCGG_T

Cuts at:   3286    3286 
   Size:       4898
 
AhdI GACnn_n'nnGTC

Cuts at:    429     429 
   Size:       4898
 
AlwNI CAG_nnn'CTG

Cuts at:    331     583    2203    3770     331 
   Size:        252    1620    1567    1459

  Fragments arranged by size: 

                1620    1567    1459     252
 
ApaI G_GGCC'C

Cuts at:    489     623    1396     489 
   Size:        134     773    3991

  Fragments arranged by size: 

                3991     773     134
 
ApaLI G'TGCA_C

Cuts at:   3668    3668 
   Size:       4898
 
ApoI r'AATT_y

Cuts at:    281     321     713     727     800    1072    1170    1902    4251 
   Size:         40     392      14      73     272      98     732    2349

Cuts at:   4251    4507     281 
   Size:        256     672

  Fragments arranged by size: 

                2349     732     672     392     272     256      98      73
                  40      14
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       4898
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4898
 
AvaI C'yCGr_G

Cuts at:    122    1918     122 
   Size:       1796    3102
 
BamHI G'GATC_C

Cuts at:   1923    1923 
   Size:       4898
 
BanI G'GyrC_C

Cuts at:   1266    1914    2328    2576    2611    1266 
   Size:        648     414     248      35    3553

  Fragments arranged by size: 

                3553     648     414     248      35
 
BanII G_rGCy'C

Cuts at:    489     510     623    1396    1912    2942     489 
   Size:         21     113     773     516    1030    2445

  Fragments arranged by size: 

                2445    1030     773     516     113      21
 
BbeI G_GCGC'C

Cuts at:   2332    2580    2332 
   Size:        248    4650
 
BbsI GAAGACnn'nnnn_

Cuts at:    614     614 
   Size:       4898
 
BbvCI CC'TCA_GC

Cuts at:   1861    1873    1861 
   Size:         12    4886
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1831    1865    1831 
   Size:         34    4864
 
BciVI GTATCCnnnnn_n'

Cuts at:   2796    3557    4898    2796 
   Size:        761    1341    2796

  Fragments arranged by size: 

                2796    1341     761
 
BclI T'GATC_A

Cuts at:   2418    2418 
   Size:       4898
 
BglI GCCn_nnn'nGGC

Cuts at:    118     118 
   Size:       4898
 
BglII A'GATC_T

Cuts at:   1482    1560    2413    4450    1482 
   Size:         78     853    2037    1930

  Fragments arranged by size: 

                2037    1930     853      78
 
BlpI GC'TnA_GC

Cuts at:    674    2010     674 
   Size:       1336    3562
 
Bme1580I G_kGCm'C

Cuts at:    489     623    1396    2523    2616    3672     489 
   Size:        134     773    1127      93    1056    1715

  Fragments arranged by size: 

                1715    1127    1056     773     134      93
 
BmrI ACTGGGnnnn_n'

Cuts at:    284     471     546     609     621    2110    2191    2526    4670 
   Size:        187      75      63      12    1489      81     335    2144

Cuts at:   4670     284 
   Size:        512

  Fragments arranged by size: 

                2144    1489     512     335     187      81      75      63
                  12
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3265    3265 
   Size:       4898
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    459     524    1840     459 
   Size:         65    1316    3517

  Fragments arranged by size: 

                3517    1316      65
 
Bpu10I CC'TnA_GC

Cuts at:    371    1861    1873     371 
   Size:       1490      12    3396

  Fragments arranged by size: 

                3396    1490      12
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   2074    2953    3445    3743    2074 
   Size:        879     492     298    3229

  Fragments arranged by size: 

                3229     879     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:   4602    4602 
   Size:       4898
 
BsaAI yAC'GTr

Cuts at:   2251    2251 
   Size:       4898
 
BsaBI GATnn'nnATC

Cuts at:      6    2412    4449       6 
   Size:       2406    2037     455

  Fragments arranged by size: 

                2406    2037     455
 
BsaHI Gr'CG_yC

Cuts at:   2329    2577    3279    4775    2329 
   Size:        248     702    1496    2452

  Fragments arranged by size: 

                2452    1496     702     248
 
BsaWI w'CCGG_w

Cuts at:    204    1781    2087    2608    3286    3560    3707    4118     204 
   Size:       1577     306     521     678     274     147     411     984

  Fragments arranged by size: 

                1577     984     678     521     411     306     274     147
            
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:    510     540     510 
   Size:         30    4868
 
BseYI C'CCAG_C

Cuts at:    479     488    2323    3081    3658    4154     479 
   Size:          9    1835     758     577     496    1223

  Fragments arranged by size: 

                1835    1223     758     577     496       9
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1371    1371 
   Size:       4898
 
BsiEI CG_ry'CG

Cuts at:    104    2440    2486    3694     104 
   Size:       2336      46    1208    1308

  Fragments arranged by size: 

                2336    1308    1208      46
 
BsiHKAI G_wGCw'C

Cuts at:   1376    1912    2690    2880    3672    1376 
   Size:        536     778     190     792    2602

  Fragments arranged by size: 

                2602     792     778     536     190
 
BsmBI CGTCTCn'nnnn_

Cuts at:   1000    1000 
   Size:       4898
 
Bsp1286I G_dGCh'C

Cuts at:    489     510     623    1376    1396    1912    2523    2616    2690 
   Size:         21     113     753      20     516     611      93      74

Cuts at:   2690    2880    2942    3672     489 
   Size:        190      62     730    1715

  Fragments arranged by size: 

                1715     753     730     611     516     190     113      93
                  74      62      21      20
 
BspEI T'CCGG_A

Cuts at:    204    4118     204 
   Size:       3914     984
 
BspHI T'CATG_A

Cuts at:   1238    4074    1238 
   Size:       2836    2062
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:   1948    2162    2464    2845    1948 
   Size:        214     302     381    4001

  Fragments arranged by size: 

                4001     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50    3190    3244    4728    4893      50 
   Size:       3140      54    1484     165      55

  Fragments arranged by size: 

                3140    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:    426    2810     426 
   Size:       2384    2514
 
BsrFI r'CCGG_y

Cuts at:   2896    3077    3286    2896 
   Size:        181     209    4508

  Fragments arranged by size: 

                4508     209     181
 
BssSI C'ACGA_G

Cuts at:   1537    1588    3169    3527    1537 
   Size:         51    1581     358    2908

  Fragments arranged by size: 

                2908    1581     358      51
 
BstAPI GCAn_nnn'nTGC

Cuts at:   1830    2275    1830 
   Size:        445    4453
 
BstBI TT'CG_AA

Cuts at:   3259    3259 
   Size:       4898
 
BstXI CCAn_nnnn'nTGG

Cuts at:   1332    1539    1332 
   Size:        207    4691
 
BstYI r'GATC_y

Cuts at:      1     873    1482    1560    1784    1923    2390    2413    2748 
   Size:        872     609      78     224     139     467      23     335

Cuts at:   2748    2994    4006    4092    4104    4450       1 
   Size:        246    1012      86      12     346     449

  Fragments arranged by size: 

                1012     872     609     467     449     346     335     246
                 224     139      86      78      23      12
 
BtgI C'CryG_G

Cuts at:    107    1253    1331     107 
   Size:       1146      78    3674

  Fragments arranged by size: 

                3674    1146      78
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   2848    3029    3192    2848 
   Size:        181     163    4554

  Fragments arranged by size: 

                4554     181     163
 
BtsI GCAGTG_nn'

Cuts at:    420     542    2141     420 
   Size:        122    1599    3177

  Fragments arranged by size: 

                3177    1599     122
 
ClaI AT'CG_AT

Cuts at:    948     948 
   Size:       4898
 
DraI TTT'AAA

Cuts at:   1897    1897 
   Size:       4898
 
DrdI GACnn_nn'nnGTC

Cuts at:   1048    2604    3462    1048 
   Size:       1556     858    2484

  Fragments arranged by size: 

                2484    1556     858
 
EaeI y'GGCC_r

Cuts at:    110     552     770    1442    2483    2657    3048    3075    4420 
   Size:        442     218     672    1041     174     391      27    1345

Cuts at:   4420     110 
   Size:        588

  Fragments arranged by size: 

                1345    1041     672     588     442     391     218     174
                  27
 
EagI C'GGCC_G

Cuts at:   2483    2483 
   Size:       4898
 
EarI CTCTTCn'nnn_

Cuts at:    636    1040    1061    1717    2921    3131     636 
   Size:        404      21     656    1204     210    2403

  Fragments arranged by size: 

                2403    1204     656     404     210      21
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   1798    3106    3416    3562    4708    1798 
   Size:       1308     310     146    1146    1988

  Fragments arranged by size: 

                1988    1308    1146     310     146
 
EcoICRI GAG'CTC

Cuts at:   1910    1910 
   Size:       4898
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    459     524    1084    1840    2723    3155    3902    4170     459 
   Size:         65     560     756     883     432     747     268    1187

  Fragments arranged by size: 

                1187     883     756     747     560     432     268      65
            
 
EcoO109I rG'GnC_Cy

Cuts at:    485    1869    2037     485 
   Size:       1384     168    3346

  Fragments arranged by size: 

                3346    1384     168
 
EcoRI G'AATT_C

Cuts at:   1902    1902 
   Size:       4898
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:    361    1621    2917     361 
   Size:       1260    1296    2342

  Fragments arranged by size: 

                2342    1296    1260
 
FspI TGC'GCA

Cuts at:   1087    2679    1087 
   Size:       1592    3306
 
FspAI rTGC'GCAy

Cuts at:   1087    1087 
   Size:       4898
 
HaeII r_GCGC'y

Cuts at:    674    1386    2332    2580    3602    4581     674 
   Size:        712     946     248    1022     979     991

  Fragments arranged by size: 

                1022     991     979     946     712     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    173     205     340     372     415     447     637     669    1718 
   Size:         32     135      32      43      32     190      32    1049

Cuts at:   1718    1750    2740    2770    2772    2802     173 
   Size:         32     990      30       2      30    2269

  Fragments arranged by size: 

                2269    1049     990     190     135      43      32      32
                  32      32      32      30      30       2
 
HincII GTy'rAC

Cuts at:   1937    1937 
   Size:       4898
 
KasI G'GCGC_C

Cuts at:   2328    2576    2328 
   Size:        248    4650
 
KpnI G_GTAC'C

Cuts at:   1918    1918 
   Size:       4898
 
MluI A'CGCG_T

Cuts at:   1745    4331    1745 
   Size:       2586    2312
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:    795    3569    3753     795 
   Size:       2774     184    1940

  Fragments arranged by size: 

                2774    1940     184
 
MscI TGG'CCA

Cuts at:    112     772    1444    2659     112 
   Size:        660     672    1215    2351

  Fragments arranged by size: 

                2351    1215     672     660
 
MslI CAynn'nnrTG

Cuts at:   2887    3014    2887 
   Size:        127    4771
 
MspA1I CmG'CkG

Cuts at:    658    2009    2323    2683    3696    3941     658 
   Size:       1351     314     360    1013     245    1615

  Fragments arranged by size: 

                1615    1351    1013     360     314     245
 
NaeI GCC'GGC

Cuts at:   3079    3079 
   Size:       4898
 
NarI GG'CG_CC

Cuts at:   2329    2577    2329 
   Size:        248    4650
 
NcoI C'CATG_G

Cuts at:    107    1253    1331     107 
   Size:       1146      78    3674

  Fragments arranged by size: 

                3674    1146      78
 
NgoMIV G'CCGG_C

Cuts at:   3077    3077 
   Size:       4898
 
NspI r_CATG'y

Cuts at:   1951    2451    3358    1951 
   Size:        500     907    3491

  Fragments arranged by size: 

                3491     907     500
 
PciI A'CATG_T

Cuts at:   3354    3354 
   Size:       4898
 
PflMI CCAn_nnn'nTGG

Cuts at:    536     583     536 
   Size:         47    4851
 
PfoI T'CCnGG_A

Cuts at:    732     732 
   Size:       4898
 
PmeI GTTT'AAAC

Cuts at:   1897    1897 
   Size:       4898
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:    245     277    4037    4069     245 
   Size:         32    3760      32    1074

  Fragments arranged by size: 

                3760    1074      32      32
 
PspOMI G'GGCC_C

Cuts at:    485     619    1392     485 
   Size:        134     773    3991

  Fragments arranged by size: 

                3991     773     134
 
PstI C_TGCA'G

Cuts at:   1945    2630    1945 
   Size:        685    4213
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       4898
 
PvuII CAG'CTG

Cuts at:    658    2323    2683     658 
   Size:       1665     360    2873

  Fragments arranged by size: 

                2873    1665     360
 
RsrII CG'GwC_CG

Cuts at:   3093    3093 
   Size:       4898
 
SacI G_AGCT'C

Cuts at:   1912    1912 
   Size:       4898
 
SalI G'TCGA_C

Cuts at:   1935    1935 
   Size:       4898
 
SapI GCTCTTCn'nnn_

Cuts at:   2921    3131    2921 
   Size:        210    4688
 
SbfI CC_TGCA'GG

Cuts at:   1945    1945 
   Size:       4898
 
ScaI AGT'ACT

Cuts at:   4415    4415 
   Size:       4898
 
SexAI A'CCwGG_T

Cuts at:    404     404 
   Size:       4898
 
SfcI C'TryA_G

Cuts at:     33    1941    2626    3619    3810      33 
   Size:       1908     685     993     191    1121

  Fragments arranged by size: 

                1908    1121     993     685     191
 
SfoI GGC'GCC

Cuts at:   2330    2578    2330 
   Size:        248    4650
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4898
 
SmaI CCC'GGG

Cuts at:   1920    1920 
   Size:       4898
 
SmlI C'TyrA_G

Cuts at:   2053    2968    3460    3722    2053 
   Size:        915     492     262    3229

  Fragments arranged by size: 

                3229     915     492     262
 
SpeI A'CTAG_T

Cuts at:     67    1674      67 
   Size:       1607    3291
 
SphI G_CATG'C

Cuts at:   1951    2451    1951 
   Size:        500    4398
 
StuI AGG'CCT

Cuts at:    565    1105    1429     565 
   Size:        540     324    4034

  Fragments arranged by size: 

                4034     540     324
 
StyI C'CwwG_G

Cuts at:    107     116     570    1253    1325    1331    1403    2032    2386 
   Size:          9     454     683      72       6      72     629     354

Cuts at:   2386     107 
   Size:       2619

  Fragments arranged by size: 

                2619     683     629     454     354      72      72       9
                   6
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:   2481    2614    3073    3282    2481 
   Size:        133     459     209    4097

  Fragments arranged by size: 

                4097     459     209     133
 
TatI w'GTAC_w

Cuts at:   4413    4413 
   Size:       4898
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:    311     343     510     542    4415    4447     311 
   Size:         32     167      32    3873      32     762

  Fragments arranged by size: 

                3873     762     167      32      32      32
 
Tth111I GACn'n_nGTC

Cuts at:   2695    2695 
   Size:       4898
 
XbaI T'CTAG_A

Cuts at:   1929    1929 
   Size:       4898
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:    412    2393     412 
   Size:       1981    2917
 
XmaI C'CCGG_G

Cuts at:   1918    1918 
   Size:       4898
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI     AfeI   AflIII     AgeI     AhdI
    AlwNI     ApaI    ApaLI     ApoI     AseI    AsiSI     AvaI    BamHI
     BanI    BanII     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI
     BglI    BglII     BlpI Bme1580I     BmrI     BplI     BpmI   Bpu10I
    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseYI
     BsgI    BsiEI  BsiHKAI    BsmBI Bsp1286I    BspEI    BspHI    BspMI
    BsrBI    BsrDI    BsrFI    BssSI   BstAPI    BstBI    BstXI    BstYI
     BtgI    BtgZI     BtsI     ClaI     DraI     DrdI     EaeI     EagI
     EarI     EciI  EcoICRI  Eco57MI EcoO109I    EcoRI     FalI     FspI
    FspAI    HaeII    Hin4I   HincII     KasI     KpnI     MluI     MmeI
     MscI     MslI   MspA1I     NaeI     NarI     NcoI   NgoMIV     NspI
     PciI    PflMI     PfoI     PmeI     PpiI   PspOMI     PstI     PvuI
    PvuII    RsrII     SacI     SalI     SapI     SbfI     ScaI    SexAI
     SfcI     SfoI     SgfI     SmaI     SmlI     SpeI     SphI     StuI
     StyI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XmaI

 Enzymes that do not cut: 

     AarI    AatII    AflII     AleI     AlfI     AloI     AscI    AvrII
     BaeI    BfrBI    BmgBI     BmtI    BseRI    BsiWI     BsmI    BsrGI
   BssHII   BstEII  BstZ17I   Bsu36I    CspCI   DraIII    EcoNI    EcoRV
     FseI  HindIII     HpaI     MfeI     NdeI     NheI     NotI     NruI
     NsiI     PacI     PasI     PmlI    PpuMI    PshAI     PsiI    PspXI
     PsrI    SacII    SanDI     SfiI    SgrAI    SnaBI     SrfI     SspI
     SwaI     XhoI     XmnI     ZraI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com