Restriction map for
pF1KB6140
(Circular) (Six-base) MAPSORT of: pF1KB6140.seq Check: 4011 from: 1 to: 5015
pF1KB6140 5015 bp
With 182 enzymes: SgfI *
June 26, 2008 10:46 ..
AccI GT'mk_AC
Cuts at: 2053 2053
Size: 5015
Acc65I G'GTAC_C
Cuts at: 2031 2031
Size: 5015
AclI AA'CG_TT
Cuts at: 4855 4855
Size: 5015
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 203 542 571 722 1069 1504 1624 2840 3272
Size: 339 29 151 347 435 120 1216 432
Cuts at: 3272 4019 4287 203
Size: 747 268 931
Fragments arranged by size:
1216 931 747 435 432 347 339 268
151 120 29
AfeI AGC'GCT
Cuts at: 149 149
Size: 5015
AflIII A'CryG_T
Cuts at: 1355 1739 2367 3471 4448 1355
Size: 384 628 1104 977 1922
Fragments arranged by size:
1922 1104 977 628 384
AgeI A'CCGG_T
Cuts at: 3403 3403
Size: 5015
AhdI GACnn_n'nnGTC
Cuts at: 384 384
Size: 5015
AleI CACnn'nnGTG
Cuts at: 1096 1096
Size: 5015
AlwNI CAG_nnn'CTG
Cuts at: 652 2320 3887 652
Size: 1668 1567 1780
Fragments arranged by size:
1780 1668 1567
ApaI G_GGCC'C
Cuts at: 353 353
Size: 5015
ApaLI G'TGCA_C
Cuts at: 1361 1918 3785 1361
Size: 557 1867 2591
Fragments arranged by size:
2591 1867 557
ApoI r'AATT_y
Cuts at: 2019 4368 4624 2019
Size: 2349 256 2410
Fragments arranged by size:
2410 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5015
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5015
AvaI C'yCGr_G
Cuts at: 444 450 923 1189 1343 1703 1724 2035 444
Size: 6 473 266 154 360 21 311 3424
Fragments arranged by size:
3424 473 360 311 266 154 21 6
BamHI G'GATC_C
Cuts at: 2040 2040
Size: 5015
BanI G'GyrC_C
Cuts at: 933 1411 2031 2445 2693 2728 933
Size: 478 620 414 248 35 3220
Fragments arranged by size:
3220 620 478 414 248 35
BanII G_rGCy'C
Cuts at: 353 451 826 861 1352 1580 1930 2029 3059
Size: 98 375 35 491 228 350 99 1030
Cuts at: 3059 353
Size: 2309
Fragments arranged by size:
2309 1030 491 375 350 228 99 98
35
BbeI G_GCGC'C
Cuts at: 1415 2449 2697 1415
Size: 1034 248 3733
Fragments arranged by size:
3733 1034 248
BbsI GAAGACnn'nnnn_
Cuts at: 1186 1870 1186
Size: 684 4331
BbvCI CC'TCA_GC
Cuts at: 289 1873 289
Size: 1584 3431
BciVI GTATCCnnnnn_n'
Cuts at: 2913 3674 5015 2913
Size: 761 1341 2913
Fragments arranged by size:
2913 1341 761
BclI T'GATC_A
Cuts at: 539 2535 539
Size: 1996 3019
BglI GCCn_nnn'nGGC
Cuts at: 925 1156 925
Size: 231 4784
BglII A'GATC_T
Cuts at: 2530 4567 2530
Size: 2037 2978
BlpI GC'TnA_GC
Cuts at: 860 2127 860
Size: 1267 3748
Bme1580I G_kGCm'C
Cuts at: 353 697 938 1365 1552 1922 2640 2733 3789
Size: 344 241 427 187 370 718 93 1056
Cuts at: 3789 353
Size: 1579
Fragments arranged by size:
1579 1056 718 427 370 344 241 187
93
BmgBI CAC'GTC
Cuts at: 414 414
Size: 5015
BmrI ACTGGGnnnn_n'
Cuts at: 932 1986 2227 2308 2643 4787 932
Size: 1054 241 81 335 2144 1160
Fragments arranged by size:
2144 1160 1054 335 241 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 459 1975 3382 459
Size: 1516 1407 2092
Fragments arranged by size:
2092 1516 1407
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 712 1112 1195 1297 1321 1674 1683 712
Size: 400 83 102 24 353 9 4044
Fragments arranged by size:
4044 400 353 102 83 24 9
Bpu10I CC'TnA_GC
Cuts at: 257 289 1073 1644 1873 257
Size: 32 784 571 229 3399
Fragments arranged by size:
3399 784 571 229 32
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1444 2191 3070 3562 3860 1444
Size: 747 879 492 298 2599
Fragments arranged by size:
2599 879 747 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 390 4719 390
Size: 4329 686
BsaAI yAC'GTr
Cuts at: 1385 2003 2368 1385
Size: 618 365 4032
Fragments arranged by size:
4032 618 365
BsaBI GATnn'nnATC
Cuts at: 6 2529 4566 6
Size: 2523 2037 455
Fragments arranged by size:
2523 2037 455
BsaHI Gr'CG_yC
Cuts at: 796 928 1412 1720 1867 2446 2694 3396 4892
Size: 132 484 308 147 579 248 702 1496
Cuts at: 4892 796
Size: 919
Fragments arranged by size:
1496 919 702 579 484 308 248 147
132
BsaWI w'CCGG_w
Cuts at: 2204 2725 3403 3677 3824 4235 2204
Size: 521 678 274 147 411 2984
Fragments arranged by size:
2984 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 660 690 893 923 1912 1942 660
Size: 30 203 30 989 30 3733
Fragments arranged by size:
3733 989 203 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 408 901 1294 1414 1920 408
Size: 493 393 120 506 3503
Fragments arranged by size:
3503 506 493 393 120
BseYI C'CCAG_C
Cuts at: 807 1038 1212 1237 2440 3198 3775 4271 807
Size: 231 174 25 1203 758 577 496 1551
Fragments arranged by size:
1551 1203 758 577 496 231 174 25
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 964 1309 1544 1720 964
Size: 345 235 176 4259
Fragments arranged by size:
4259 345 235 176
BsiEI CG_ry'CG
Cuts at: 104 1148 1328 1937 2557 2603 3811 104
Size: 1044 180 609 620 46 1208 1308
Fragments arranged by size:
1308 1208 1044 620 609 180 46
BsiHKAI G_wGCw'C
Cuts at: 425 664 826 1365 1472 1922 1930 2029 2807
Size: 239 162 539 107 450 8 99 778
Cuts at: 2807 2997 3789 425
Size: 190 792 1651
Fragments arranged by size:
1651 792 778 539 450 239 190 162
107 99 8
Bsp1286I G_dGCh'C
Cuts at: 353 425 451 664 697 826 861 938 1352
Size: 72 26 213 33 129 35 77 414
Cuts at: 1352 1365 1472 1552 1580 1922 1930 2029 2640
Size: 13 107 80 28 342 8 99 611
Cuts at: 2640 2733 2807 2997 3059 3789 353
Size: 93 74 190 62 730 1579
Fragments arranged by size:
1579 730 611 414 342 213 190 129
107 99 93 80 77 74 72 62
35 33 28 26 13 8
BspEI T'CCGG_A
Cuts at: 4235 4235
Size: 5015
BspHI T'CATG_A
Cuts at: 506 4191 506
Size: 3685 1330
BspMI ACCTGCnnnn'nnnn_
Cuts at: 259 1599 2065 2279 2581 2962 259
Size: 1340 466 214 302 381 2312
Fragments arranged by size:
2312 1340 466 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1072 1409 1643 1982 3307 3361 4845 5010
Size: 1022 337 234 339 1325 54 1484 165
Cuts at: 5010 50
Size: 55
Fragments arranged by size:
1484 1325 1022 339 337 234 165 55
54
BsrDI GCAATG_nn'
Cuts at: 2927 2927
Size: 5015
BsrFI r'CCGG_y
Cuts at: 1478 1801 1831 3013 3194 3403 1478
Size: 323 30 1182 181 209 3090
Fragments arranged by size:
3090 1182 323 209 181 30
BssSI C'ACGA_G
Cuts at: 583 3286 3644 583
Size: 2703 358 1954
Fragments arranged by size:
2703 1954 358
BstAPI GCAn_nnn'nTGC
Cuts at: 2392 2392
Size: 5015
BstBI TT'CG_AA
Cuts at: 3376 3376
Size: 5015
BstEII G'GTnAC_C
Cuts at: 1110 1110
Size: 5015
BstXI CCAn_nnnn'nTGG
Cuts at: 312 318 1098 1788 312
Size: 6 780 690 3539
Fragments arranged by size:
3539 780 690 6
BstYI r'GATC_y
Cuts at: 1 1193 2040 2507 2530 2865 3111 4123 4209
Size: 1192 847 467 23 335 246 1012 86
Cuts at: 4209 4221 4567 1
Size: 12 346 449
Fragments arranged by size:
1192 1012 847 467 449 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 305 311 317 353 470 1937 305
Size: 6 6 36 117 1467 3383
Fragments arranged by size:
3383 1467 117 36 6 6
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 359 2965 3146 3309 359
Size: 2606 181 163 2065
Fragments arranged by size:
2606 2065 181 163
BtsI GCAGTG_nn'
Cuts at: 762 2258 762
Size: 1496 3519
DraI TTT'AAA
Cuts at: 227 2014 227
Size: 1787 3228
DraIII CAC_nnn'GTG
Cuts at: 376 1599 376
Size: 1223 3792
DrdI GACnn_nn'nnGTC
Cuts at: 2721 3579 2721
Size: 858 4157
EaeI y'GGCC_r
Cuts at: 308 314 1145 1325 1712 1778 1934 2600 2774
Size: 6 831 180 387 66 156 666 174
Cuts at: 2774 3165 3192 4537 308
Size: 391 27 1345 786
Fragments arranged by size:
1345 831 786 666 391 387 180 174
156 66 27 6
EagI C'GGCC_G
Cuts at: 1145 1325 1934 2600 1145
Size: 180 609 666 3560
Fragments arranged by size:
3560 666 609 180
EarI CTCTTCn'nnn_
Cuts at: 741 1399 3038 3248 741
Size: 658 1639 210 2508
Fragments arranged by size:
2508 1639 658 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3223 3533 3679 4825 3223
Size: 310 146 1146 3413
Fragments arranged by size:
3413 1146 310 146
EcoICRI GAG'CTC
Cuts at: 824 1928 2027 824
Size: 1104 99 3812
Fragments arranged by size:
3812 1104 99
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 203 542 571 712 722 1069 1112 1195 1297
Size: 339 29 141 10 347 43 83 102
Cuts at: 1297 1321 1504 1624 1674 1683 2840 3272 4019
Size: 24 183 120 50 9 1157 432 747
Cuts at: 4019 4287 203
Size: 268 931
Fragments arranged by size:
1157 931 747 432 347 339 268 183
141 120 102 83 50 43 29 24
10 9
EcoNI CCTnn'n_nnAGG
Cuts at: 1279 1543 1279
Size: 264 4751
EcoO109I rG'GnC_Cy
Cuts at: 159 501 975 1886 2154 159
Size: 342 474 911 268 3020
Fragments arranged by size:
3020 911 474 342 268
EcoRI G'AATT_C
Cuts at: 2019 2019
Size: 5015
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1395 1509 3034 1395
Size: 114 1525 3376
Fragments arranged by size:
3376 1525 114
FspI TGC'GCA
Cuts at: 364 1165 2796 364
Size: 801 1631 2583
Fragments arranged by size:
2583 1631 801
FspAI rTGC'GCAy
Cuts at: 364 364
Size: 5015
HaeII r_GCGC'y
Cuts at: 151 1162 1244 1415 1459 1507 1832 1847 2449
Size: 1011 82 171 44 48 325 15 602
Cuts at: 2449 2697 3719 4698 151
Size: 248 1022 979 468
Fragments arranged by size:
1022 1011 979 602 468 325 248 171
82 48 44 15
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 370 402 660 692 947 979 2857 2887 2889
Size: 32 258 32 255 32 1878 30 2
Cuts at: 2889 2919 370
Size: 30 2466
Fragments arranged by size:
2466 1878 258 255 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1020 2054 1020
Size: 1034 3981
KasI G'GCGC_C
Cuts at: 1411 2445 2693 1411
Size: 1034 248 3733
Fragments arranged by size:
3733 1034 248
KpnI G_GTAC'C
Cuts at: 2035 2035
Size: 5015
MluI A'CGCG_T
Cuts at: 4448 4448
Size: 5015
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 298 452 1877 3686 3870 298
Size: 154 1425 1809 184 1443
Fragments arranged by size:
1809 1443 1425 184 154
MscI TGG'CCA
Cuts at: 316 2776 316
Size: 2460 2555
MslI CAynn'nnrTG
Cuts at: 247 310 316 1096 3004 3131 247
Size: 63 6 780 1908 127 2131
Fragments arranged by size:
2131 1908 780 127 63 6
MspA1I CmG'CkG
Cuts at: 549 1173 1391 1533 1585 1698 2126 2440 2800
Size: 624 218 142 52 113 428 314 360
Cuts at: 2800 3813 4058 549
Size: 1013 245 1506
Fragments arranged by size:
1506 1013 624 428 360 314 245 218
142 113 52
NaeI GCC'GGC
Cuts at: 1480 1833 3196 1480
Size: 353 1363 3299
Fragments arranged by size:
3299 1363 353
NarI GG'CG_CC
Cuts at: 1412 2446 2694 1412
Size: 1034 248 3733
Fragments arranged by size:
3733 1034 248
NcoI C'CATG_G
Cuts at: 311 317 311
Size: 6 5009
NgoMIV G'CCGG_C
Cuts at: 1478 1831 3194 1478
Size: 353 1363 3299
Fragments arranged by size:
3299 1363 353
NotI GC'GGCC_GC
Cuts at: 1145 1145
Size: 5015
NspI r_CATG'y
Cuts at: 125 363 1359 2068 2568 3475 125
Size: 238 996 709 500 907 1665
Fragments arranged by size:
1665 996 907 709 500 238
PciI A'CATG_T
Cuts at: 1355 3471 1355
Size: 2116 2899
PfoI T'CCnGG_A
Cuts at: 1961 1961
Size: 5015
PmeI GTTT'AAAC
Cuts at: 2014 2014
Size: 5015
PmlI CAC'GTG
Cuts at: 1385 2003 1385
Size: 618 4397
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1759 1791 4154 4186 1759
Size: 32 2363 32 2588
Fragments arranged by size:
2588 2363 32 32
PpuMI rG'GwC_Cy
Cuts at: 159 1886 159
Size: 1727 3288
PspOMI G'GGCC_C
Cuts at: 349 349
Size: 5015
PspXI vC'TCGA_Gb
Cuts at: 444 444
Size: 5015
PstI C_TGCA'G
Cuts at: 1511 1544 1766 2062 2747 1511
Size: 33 222 296 685 3779
Fragments arranged by size:
3779 685 296 222 33
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5015
PvuII CAG'CTG
Cuts at: 549 1173 1698 2440 2800 549
Size: 624 525 742 360 2764
Fragments arranged by size:
2764 742 624 525 360
RsrII CG'GwC_CG
Cuts at: 3210 3210
Size: 5015
SacI G_AGCT'C
Cuts at: 826 1930 2029 826
Size: 1104 99 3812
Fragments arranged by size:
3812 1104 99
SalI G'TCGA_C
Cuts at: 2052 2052
Size: 5015
SanDI GG'GwC_CC
Cuts at: 159 1886 159
Size: 1727 3288
SapI GCTCTTCn'nnn_
Cuts at: 1399 3038 3248 1399
Size: 1639 210 3166
Fragments arranged by size:
3166 1639 210
SbfI CC_TGCA'GG
Cuts at: 2062 2062
Size: 5015
ScaI AGT'ACT
Cuts at: 4532 4532
Size: 5015
SexAI A'CCwGG_T
Cuts at: 1265 1265
Size: 5015
SfcI C'TryA_G
Cuts at: 33 1507 1540 1681 1762 2058 2743 3736 3927
Size: 1474 33 141 81 296 685 993 191
Cuts at: 3927 33
Size: 1121
Fragments arranged by size:
1474 1121 993 685 296 191 141 81
33
SfoI GGC'GCC
Cuts at: 1413 2447 2695 1413
Size: 1034 248 3733
Fragments arranged by size:
3733 1034 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5015
SmaI CCC'GGG
Cuts at: 925 1345 1705 2037 925
Size: 420 360 332 3903
Fragments arranged by size:
3903 420 360 332
SmlI C'TyrA_G
Cuts at: 444 1189 1459 2170 3085 3577 3839 444
Size: 745 270 711 915 492 262 1620
Fragments arranged by size:
1620 915 745 711 492 270 262
SpeI A'CTAG_T
Cuts at: 67 1093 67
Size: 1026 3989
SphI G_CATG'C
Cuts at: 125 2068 2568 125
Size: 1943 500 2572
Fragments arranged by size:
2572 1943 500
StuI AGG'CCT
Cuts at: 1687 1687
Size: 5015
StyI C'CwwG_G
Cuts at: 279 311 317 1568 2149 2503 279
Size: 32 6 1251 581 354 2791
Fragments arranged by size:
2791 1251 581 354 32 6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 127 1218 1770 2598 2731 3190 3399 127
Size: 1091 552 828 133 459 209 1743
Fragments arranged by size:
1743 1091 828 552 459 209 133
TatI w'GTAC_w
Cuts at: 4530 4530
Size: 5015
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 893 925 1369 1401 1731 1763 1912 1944 4532
Size: 32 444 32 330 32 149 32 2588
Cuts at: 4532 4564 893
Size: 32 1344
Fragments arranged by size:
2588 1344 444 330 149 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 2812 2812
Size: 5015
XbaI T'CTAG_A
Cuts at: 2046 2046
Size: 5015
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2510 2510
Size: 5015
XhoI C'TCGA_G
Cuts at: 444 1189 444
Size: 745 4270
XmaI C'CCGG_G
Cuts at: 923 1343 1703 2035 923
Size: 420 360 332 3903
Fragments arranged by size:
3903 420 360 332
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AhdI
AleI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI FspAI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NotI NspI PciI PfoI PmeI PmlI
PpiI PpuMI PspOMI PspXI PstI PvuI PvuII RsrII
SacI SalI SanDI SapI SbfI ScaI SexAI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AarI AatII AflII AlfI AloI AscI AvrII BaeI
BcgI BfrBI BmtI BsiWI BsmI BsmBI BsrGI BssHII
BstZ17I Bsu36I ClaI CspCI EcoRV FseI HindIII HpaI
MfeI NdeI NheI NruI NsiI PacI PasI PflMI
PshAI PsiI PsrI SacII SfiI SgrAI SnaBI SrfI
SspI SwaI XmnI ZraI