Restriction map for
pF1KB6129
(Circular) (Six-base) MAPSORT of: pF1KB6129.seq Check: 8610 from: 1 to: 4874
pF1KB6129 4874 bp
With 182 enzymes: SgfI *
June 26, 2008 10:46 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1271 1271
Size: 4874
AccI GT'mk_AC
Cuts at: 413 1912 413
Size: 1499 3375
Acc65I G'GTAC_C
Cuts at: 1890 1890
Size: 4874
AclI AA'CG_TT
Cuts at: 4714 4714
Size: 4874
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 332 1543 2699 3131 3878 4146 332
Size: 1211 1156 432 747 268 1060
Fragments arranged by size:
1211 1156 1060 747 432 268
AflII C'TTAA_G
Cuts at: 1699 1699
Size: 4874
AflIII A'CryG_T
Cuts at: 887 1824 2226 3330 4307 887
Size: 937 402 1104 977 1454
Fragments arranged by size:
1454 1104 977 937 402
AgeI A'CCGG_T
Cuts at: 3262 3262
Size: 4874
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1033 1065 1033
Size: 32 4842
AlwNI CAG_nnn'CTG
Cuts at: 987 2179 3746 987
Size: 1192 1567 2115
Fragments arranged by size:
2115 1567 1192
ApaLI G'TGCA_C
Cuts at: 3644 3644
Size: 4874
ApoI r'AATT_y
Cuts at: 644 1878 4227 4483 644
Size: 1234 2349 256 1035
Fragments arranged by size:
2349 1234 1035 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4874
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4874
AvaI C'yCGr_G
Cuts at: 418 623 917 1894 418
Size: 205 294 977 3398
Fragments arranged by size:
3398 977 294 205
BamHI G'GATC_C
Cuts at: 1172 1899 1172
Size: 727 4147
BanI G'GyrC_C
Cuts at: 1890 2304 2552 2587 1890
Size: 414 248 35 4177
Fragments arranged by size:
4177 414 248 35
BanII G_rGCy'C
Cuts at: 261 1652 1751 1764 1888 2918 261
Size: 1391 99 13 124 1030 2217
Fragments arranged by size:
2217 1391 1030 124 99 13
BbeI G_GCGC'C
Cuts at: 2308 2556 2308
Size: 248 4626
BbsI GAAGACnn'nnnn_
Cuts at: 338 365 610 1507 338
Size: 27 245 897 3705
Fragments arranged by size:
3705 897 245 27
BbvCI CC'TCA_GC
Cuts at: 187 324 1378 1797 187
Size: 137 1054 419 3264
Fragments arranged by size:
3264 1054 419 137
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 793 827 1375 1409 90
Size: 34 669 34 548 34 3555
Fragments arranged by size:
3555 669 548 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2772 3533 4874 2772
Size: 761 1341 2772
Fragments arranged by size:
2772 1341 761
BclI T'GATC_A
Cuts at: 2394 2394
Size: 4874
BglII A'GATC_T
Cuts at: 2389 4426 2389
Size: 2037 2837
BlpI GC'TnA_GC
Cuts at: 560 1986 560
Size: 1426 3448
Bme1580I G_kGCm'C
Cuts at: 204 2499 2592 3648 204
Size: 2295 93 1056 1430
Fragments arranged by size:
2295 1430 1056 93
BmgBI CAC'GTC
Cuts at: 851 1428 851
Size: 577 4297
BmrI ACTGGGnnnn_n'
Cuts at: 227 1623 2086 2167 2502 4646 227
Size: 1396 463 81 335 2144 455
Fragments arranged by size:
2144 1396 463 455 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3241 3241
Size: 4874
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 261 1134 261
Size: 873 4001
Bpu10I CC'TnA_GC
Cuts at: 187 324 1378 1750 1797 187
Size: 137 1054 372 47 3264
Fragments arranged by size:
3264 1054 372 137 47
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2050 2929 3421 3719 2050
Size: 879 492 298 3205
Fragments arranged by size:
3205 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 149 415 799 1152 4578 149
Size: 266 384 353 3426 445
Fragments arranged by size:
3426 445 384 353 266
BsaAI yAC'GTr
Cuts at: 2227 2227
Size: 4874
BsaBI GATnn'nnATC
Cuts at: 6 2388 4425 6
Size: 2382 2037 455
Fragments arranged by size:
2382 2037 455
BsaHI Gr'CG_yC
Cuts at: 2305 2553 3255 4751 2305
Size: 248 702 1496 2428
Fragments arranged by size:
2428 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2063 2584 3262 3536 3683 4094 2063
Size: 521 678 274 147 411 2843
Fragments arranged by size:
2843 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 328 358 1033 1063 328
Size: 30 675 30 4139
Fragments arranged by size:
4139 675 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 320 691 945 320
Size: 371 254 4249
Fragments arranged by size:
4249 371 254
BseYI C'CCAG_C
Cuts at: 1763 2299 3057 3634 4130 1763
Size: 536 758 577 496 2507
Fragments arranged by size:
2507 758 577 536 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 157 216 1079 157
Size: 59 863 3952
Fragments arranged by size:
3952 863 59
BsiEI CG_ry'CG
Cuts at: 104 2416 2462 3670 104
Size: 2312 46 1208 1308
Fragments arranged by size:
2312 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 261 1888 2666 2856 3648 261
Size: 1627 778 190 792 1487
Fragments arranged by size:
1627 1487 792 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 1405 1405
Size: 4874
Bsp1286I G_dGCh'C
Cuts at: 204 261 1652 1751 1764 1888 2499 2592 2666
Size: 57 1391 99 13 124 611 93 74
Cuts at: 2666 2856 2918 3648 204
Size: 190 62 730 1430
Fragments arranged by size:
1430 1391 730 611 190 124 99 93
74 62 57 13
BspEI T'CCGG_A
Cuts at: 4094 4094
Size: 4874
BspHI T'CATG_A
Cuts at: 377 1230 4050 377
Size: 853 2820 1201
Fragments arranged by size:
2820 1201 853
BspMI ACCTGCnnnn'nnnn_
Cuts at: 500 1271 1924 2138 2440 2821 500
Size: 771 653 214 302 381 2553
Fragments arranged by size:
2553 771 653 381 302 214
BsrBI CCG'CTC
Cuts at: 50 620 3166 3220 4704 4869 50
Size: 570 2546 54 1484 165 55
Fragments arranged by size:
2546 1484 570 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1391 2786 1391
Size: 1395 3479
BsrFI r'CCGG_y
Cuts at: 2872 3053 3262 2872
Size: 181 209 4484
Fragments arranged by size:
4484 209 181
BssSI C'ACGA_G
Cuts at: 3145 3503 3145
Size: 358 4516
BstAPI GCAn_nnn'nTGC
Cuts at: 2251 2251
Size: 4874
BstBI TT'CG_AA
Cuts at: 3235 3235
Size: 4874
BstYI r'GATC_y
Cuts at: 1 1158 1172 1256 1899 2366 2389 2724 2970
Size: 1157 14 84 643 467 23 335 246
Cuts at: 2970 3982 4068 4080 4426 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1157 1012 643 467 449 346 335 246
86 84 23 14 12
Bsu36I CC'TnA_GG
Cuts at: 332 332
Size: 4874
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2824 3005 3168 2824
Size: 181 163 4530
Fragments arranged by size:
4530 181 163
BtsI GCAGTG_nn'
Cuts at: 1067 2117 1067
Size: 1050 3824
DraI TTT'AAA
Cuts at: 314 1873 314
Size: 1559 3315
DrdI GACnn_nn'nnGTC
Cuts at: 2580 3438 2580
Size: 858 4016
EaeI y'GGCC_r
Cuts at: 2459 2633 3024 3051 4396 2459
Size: 174 391 27 1345 2937
Fragments arranged by size:
2937 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2459 2459
Size: 4874
EarI CTCTTCn'nnn_
Cuts at: 475 1535 1860 2897 3107 475
Size: 1060 325 1037 210 2242
Fragments arranged by size:
2242 1060 1037 325 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3082 3392 3538 4684 3082
Size: 310 146 1146 3272
Fragments arranged by size:
3272 1146 310 146
EcoICRI GAG'CTC
Cuts at: 259 1886 259
Size: 1627 3247
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 261 332 1134 1543 2699 3131 3878 4146 261
Size: 71 802 409 1156 432 747 268 989
Fragments arranged by size:
1156 989 802 747 432 409 268 71
EcoNI CCTnn'n_nnAGG
Cuts at: 511 511
Size: 4874
EcoO109I rG'GnC_Cy
Cuts at: 735 2013 735
Size: 1278 3596
EcoRI G'AATT_C
Cuts at: 644 1878 644
Size: 1234 3640
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1018 1135 1343 1725 2893 1018
Size: 117 208 382 1168 2999
Fragments arranged by size:
2999 1168 382 208 117
FspI TGC'GCA
Cuts at: 2655 2655
Size: 4874
HaeII r_GCGC'y
Cuts at: 842 2308 2556 3578 4557 842
Size: 1466 248 1022 979 1159
Fragments arranged by size:
1466 1159 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 259 291 328 360 1132 1164 1229 1261 1814
Size: 32 37 32 772 32 65 32 553
Cuts at: 1814 1846 2716 2746 2748 2778 259
Size: 32 870 30 2 30 2355
Fragments arranged by size:
2355 870 772 553 65 37 32 32
32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1913 1913
Size: 4874
HindIII A'AGCT_T
Cuts at: 970 1325 970
Size: 355 4519
KasI G'GCGC_C
Cuts at: 2304 2552 2304
Size: 248 4626
KpnI G_GTAC'C
Cuts at: 1894 1894
Size: 4874
MluI A'CGCG_T
Cuts at: 4307 4307
Size: 4874
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1274 3545 3729 1274
Size: 2271 184 2419
Fragments arranged by size:
2419 2271 184
MscI TGG'CCA
Cuts at: 2635 2635
Size: 4874
MslI CAynn'nnrTG
Cuts at: 2863 2990 2863
Size: 127 4747
MspA1I CmG'CkG
Cuts at: 1224 1985 2299 2659 3672 3917 1224
Size: 761 314 360 1013 245 2181
Fragments arranged by size:
2181 1013 761 360 314 245
NaeI GCC'GGC
Cuts at: 3055 3055
Size: 4874
NarI GG'CG_CC
Cuts at: 2305 2553 2305
Size: 248 4626
NgoMIV G'CCGG_C
Cuts at: 3053 3053
Size: 4874
NspI r_CATG'y
Cuts at: 542 891 1828 1927 2427 3334 542
Size: 349 937 99 500 907 2082
Fragments arranged by size:
2082 937 907 500 349 99
PasI CC'CwG_GG
Cuts at: 1744 1744
Size: 4874
PciI A'CATG_T
Cuts at: 887 1824 3330 887
Size: 937 1506 2431
Fragments arranged by size:
2431 1506 937
PfoI T'CCnGG_A
Cuts at: 1448 1535 1448
Size: 87 4787
PmeI GTTT'AAAC
Cuts at: 1873 1873
Size: 4874
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1033 1065 4013 4045 1033
Size: 32 2948 32 1862
Fragments arranged by size:
2948 1862 32 32
PpuMI rG'GwC_Cy
Cuts at: 735 735
Size: 4874
PstI C_TGCA'G
Cuts at: 1223 1837 1921 2606 1223
Size: 614 84 685 3491
Fragments arranged by size:
3491 685 614 84
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4874
PvuII CAG'CTG
Cuts at: 1224 2299 2659 1224
Size: 1075 360 3439
Fragments arranged by size:
3439 1075 360
RsrII CG'GwC_CG
Cuts at: 3069 3069
Size: 4874
SacI G_AGCT'C
Cuts at: 261 1888 261
Size: 1627 3247
SalI G'TCGA_C
Cuts at: 1911 1911
Size: 4874
SapI GCTCTTCn'nnn_
Cuts at: 2897 3107 2897
Size: 210 4664
SbfI CC_TGCA'GG
Cuts at: 1921 1921
Size: 4874
ScaI AGT'ACT
Cuts at: 4391 4391
Size: 4874
SfcI C'TryA_G
Cuts at: 33 1219 1485 1833 1917 2602 3595 3786 33
Size: 1186 266 348 84 685 993 191 1121
Fragments arranged by size:
1186 1121 993 685 348 266 191 84
SfoI GGC'GCC
Cuts at: 2306 2554 2306
Size: 248 4626
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4874
SmaI CCC'GGG
Cuts at: 625 919 1896 625
Size: 294 977 3603
Fragments arranged by size:
3603 977 294
SmlI C'TyrA_G
Cuts at: 418 1699 2029 2944 3436 3698 418
Size: 1281 330 915 492 262 1594
Fragments arranged by size:
1594 1281 915 492 330 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4874
SphI G_CATG'C
Cuts at: 1927 2427 1927
Size: 500 4374
StyI C'CwwG_G
Cuts at: 1196 2008 2362 1196
Size: 812 354 3708
Fragments arranged by size:
3708 812 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 309 2457 2590 3049 3258 309
Size: 2148 133 459 209 1925
Fragments arranged by size:
2148 1925 459 209 133
TatI w'GTAC_w
Cuts at: 1460 4389 1460
Size: 2929 1945
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4391 4423 4391
Size: 32 4842
Tth111I GACn'n_nGTC
Cuts at: 2671 2671
Size: 4874
XbaI T'CTAG_A
Cuts at: 954 1905 954
Size: 951 3923
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2369 2369
Size: 4874
XhoI C'TCGA_G
Cuts at: 418 418
Size: 4874
XmaI C'CCGG_G
Cuts at: 623 917 1894 623
Size: 294 977 3603
Fragments arranged by size:
3603 977 294
XmnI GAAnn'nnTTC
Cuts at: 268 1361 268
Size: 1093 3781
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflII AflIII AgeI
AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI
Bsu36I BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NgoMIV NspI PasI
PciI PfoI PmeI PpiI PpuMI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AhdI AleI AlfI ApaI AscI AvrII
BaeI BfrBI BglI BmtI BsiWI BsmI BsrGI BssHII
BstEII BstXI BstZ17I BtgI ClaI CspCI DraIII EcoRV
FseI FspAI HpaI MfeI NcoI NdeI NheI NotI
NruI NsiI PacI PflMI PmlI PshAI PsiI PspOMI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI SspI StuI SwaI ZraI