Restriction map for
pF1KB6118
(Circular) (Six-base) MAPSORT of: pF1KB6118.seq Check: 9643 from: 1 to: 4751
pF1KB6118 4751 bp
With 182 enzymes: SgfI *
October 7, 2009 14:19 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 501 501
Size: 4751
AccI GT'mk_AC
Cuts at: 635 1789 635
Size: 1154 3597
Acc65I G'GTAC_C
Cuts at: 1767 1767
Size: 4751
AclI AA'CG_TT
Cuts at: 4591 4591
Size: 4751
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2576 3008 3755 4023 2576
Size: 432 747 268 3304
Fragments arranged by size:
3304 747 432 268
AfeI AGC'GCT
Cuts at: 407 407
Size: 4751
AflIII A'CryG_T
Cuts at: 2103 3207 4184 2103
Size: 1104 977 2670
Fragments arranged by size:
2670 1104 977
AgeI A'CCGG_T
Cuts at: 3139 3139
Size: 4751
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1151 1151
Size: 4751
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 748 780 748
Size: 32 4719
AlwNI CAG_nnn'CTG
Cuts at: 288 456 1466 2056 3623 288
Size: 168 1010 590 1567 1416
Fragments arranged by size:
1567 1416 1010 590 168
ApaI G_GGCC'C
Cuts at: 1097 1335 1097
Size: 238 4513
ApaLI G'TGCA_C
Cuts at: 514 601 3521 514
Size: 87 2920 1744
Fragments arranged by size:
2920 1744 87
ApoI r'AATT_y
Cuts at: 1132 1735 1755 4104 4360 1132
Size: 603 20 2349 256 1523
Fragments arranged by size:
2349 1523 603 256 20
AseI AT'TA_AT
Cuts at: 20 20
Size: 4751
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4751
AvaI C'yCGr_G
Cuts at: 156 1089 1714 1771 156
Size: 933 625 57 3136
Fragments arranged by size:
3136 933 625 57
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1019 1052 1019
Size: 33 4718
BamHI G'GATC_C
Cuts at: 320 1776 320
Size: 1456 3295
BanI G'GyrC_C
Cuts at: 160 491 801 1121 1264 1767 2181 2429 2464
Size: 331 310 320 143 503 414 248 35
Cuts at: 2464 160
Size: 2447
Fragments arranged by size:
2447 503 414 331 320 310 248 143
35
BanII G_rGCy'C
Cuts at: 700 854 1097 1335 1765 2795 700
Size: 154 243 238 430 1030 2656
Fragments arranged by size:
2656 1030 430 243 238 154
BbeI G_GCGC'C
Cuts at: 164 495 2185 2433 164
Size: 331 1690 248 2482
Fragments arranged by size:
2482 1690 331 248
BbsI GAAGACnn'nnnn_
Cuts at: 1559 1559
Size: 4751
BbvCI CC'TCA_GC
Cuts at: 1233 1578 1233
Size: 345 4406
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 600 634 637 671 600
Size: 34 3 34 4680
Fragments arranged by size:
4680 34 34 3
BciVI GTATCCnnnnn_n'
Cuts at: 2649 3410 4751 2649
Size: 761 1341 2649
Fragments arranged by size:
2649 1341 761
BclI T'GATC_A
Cuts at: 2271 2271
Size: 4751
BglI GCCn_nnn'nGGC
Cuts at: 489 1119 1537 489
Size: 630 418 3703
Fragments arranged by size:
3703 630 418
BglII A'GATC_T
Cuts at: 2266 4303 2266
Size: 2037 2714
BlpI GC'TnA_GC
Cuts at: 398 1863 398
Size: 1465 3286
Bme1580I G_kGCm'C
Cuts at: 518 605 799 806 1097 1124 1269 1277 1335
Size: 87 194 7 291 27 145 8 58
Cuts at: 1335 2376 2469 3525 518
Size: 1041 93 1056 1744
Fragments arranged by size:
1744 1056 1041 291 194 145 93 87
58 27 8 7
BmgBI CAC'GTC
Cuts at: 1347 1347
Size: 4751
BmrI ACTGGGnnnn_n'
Cuts at: 1403 1476 1963 2044 2379 4523 1403
Size: 73 487 81 335 2144 1631
Fragments arranged by size:
2144 1631 487 335 81 73
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3118 3118
Size: 4751
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 844 844
Size: 4751
Bpu10I CC'TnA_GC
Cuts at: 1233 1578 1233
Size: 345 4406
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1927 2806 3298 3596 1927
Size: 879 492 298 3082
Fragments arranged by size:
3082 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 160 1395 4455 160
Size: 1235 3060 456
Fragments arranged by size:
3060 1235 456
BsaAI yAC'GTr
Cuts at: 2104 2104
Size: 4751
BsaBI GATnn'nnATC
Cuts at: 6 2265 4302 6
Size: 2259 2037 455
Fragments arranged by size:
2259 2037 455
BsaHI Gr'CG_yC
Cuts at: 161 364 492 577 2182 2430 3132 4628 161
Size: 203 128 85 1605 248 702 1496 284
Fragments arranged by size:
1605 1496 702 284 248 203 128 85
BsaWI w'CCGG_w
Cuts at: 169 689 1100 1940 2461 3139 3413 3560 3971
Size: 520 411 840 521 678 274 147 411
Cuts at: 3971 169
Size: 949
Fragments arranged by size:
949 840 678 521 520 411 411 274
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 181 211 1294 1324 181
Size: 30 1083 30 3608
Fragments arranged by size:
3608 1083 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 151 448 708 804 1221 1297 1566 1623 151
Size: 297 260 96 417 76 269 57 3279
Fragments arranged by size:
3279 417 297 269 260 96 76 57
BseYI C'CCAG_C
Cuts at: 281 700 1602 1609 2176 2934 3511 4007 281
Size: 419 902 7 567 758 577 496 1025
Fragments arranged by size:
1025 902 758 577 567 496 419 7
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 448 1388 448
Size: 940 3811
BsiEI CG_ry'CG
Cuts at: 104 522 2293 2339 3547 104
Size: 418 1771 46 1208 1308
Fragments arranged by size:
1771 1308 1208 418 46
BsiHKAI G_wGCw'C
Cuts at: 518 605 854 1030 1765 2543 2733 3525 518
Size: 87 249 176 735 778 190 792 1744
Fragments arranged by size:
1744 792 778 735 249 190 176 87
Bsp1286I G_dGCh'C
Cuts at: 518 605 700 799 806 854 1030 1097 1124
Size: 87 95 99 7 48 176 67 27
Cuts at: 1124 1269 1277 1335 1765 2376 2469 2543 2733
Size: 145 8 58 430 611 93 74 190
Cuts at: 2733 2795 3525 518
Size: 62 730 1744
Fragments arranged by size:
1744 730 611 430 190 176 145 99
95 93 87 74 67 62 58 48
27 8 7
BspEI T'CCGG_A
Cuts at: 689 1100 3971 689
Size: 411 2871 1469
Fragments arranged by size:
2871 1469 411
BspHI T'CATG_A
Cuts at: 3927 3927
Size: 4751
BspMI ACCTGCnnnn'nnnn_
Cuts at: 501 545 1801 2015 2317 2698 501
Size: 44 1256 214 302 381 2554
Fragments arranged by size:
2554 1256 381 302 214 44
BsrBI CCG'CTC
Cuts at: 50 1074 3043 3097 4581 4746 50
Size: 1024 1969 54 1484 165 55
Fragments arranged by size:
1969 1484 1024 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2663 2663
Size: 4751
BsrFI r'CCGG_y
Cuts at: 521 2749 2930 3139 521
Size: 2228 181 209 2133
Fragments arranged by size:
2228 2133 209 181
BssSI C'ACGA_G
Cuts at: 201 853 1589 3022 3380 201
Size: 652 736 1433 358 1572
Fragments arranged by size:
1572 1433 736 652 358
BstAPI GCAn_nnn'nTGC
Cuts at: 456 1402 2128 456
Size: 946 726 3079
Fragments arranged by size:
3079 946 726
BstBI TT'CG_AA
Cuts at: 3112 3112
Size: 4751
BstEII G'GTnAC_C
Cuts at: 1518 1518
Size: 4751
BstXI CCAn_nnnn'nTGG
Cuts at: 1051 1051
Size: 4751
BstYI r'GATC_y
Cuts at: 1 320 452 929 1776 2243 2266 2601 2847
Size: 319 132 477 847 467 23 335 246
Cuts at: 2847 3859 3945 3957 4303 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 847 477 467 449 346 335 319
246 132 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 667 667
Size: 4751
BtgI C'CryG_G
Cuts at: 107 1208 107
Size: 1101 3650
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2701 2882 3045 2701
Size: 181 163 4407
Fragments arranged by size:
4407 181 163
BtsI GCAGTG_nn'
Cuts at: 213 472 1028 1498 1994 213
Size: 259 556 470 496 2970
Fragments arranged by size:
2970 556 496 470 259
ClaI AT'CG_AT
Cuts at: 1316 1316
Size: 4751
DraI TTT'AAA
Cuts at: 1750 1750
Size: 4751
DrdI GACnn_nn'nnGTC
Cuts at: 174 2457 3315 174
Size: 2283 858 1610
Fragments arranged by size:
2283 1610 858
EaeI y'GGCC_r
Cuts at: 519 1299 2336 2510 2901 2928 4273 519
Size: 780 1037 174 391 27 1345 997
Fragments arranged by size:
1345 1037 997 780 391 174 27
EagI C'GGCC_G
Cuts at: 519 2336 519
Size: 1817 2934
EarI CTCTTCn'nnn_
Cuts at: 218 440 2774 2984 218
Size: 222 2334 210 1985
Fragments arranged by size:
2334 1985 222 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 393 2959 3269 3415 4561 393
Size: 2566 310 146 1146 583
Fragments arranged by size:
2566 1146 583 310 146
EcoICRI GAG'CTC
Cuts at: 852 1763 852
Size: 911 3840
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 844 2576 3008 3755 4023 844
Size: 1732 432 747 268 1572
Fragments arranged by size:
1732 1572 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 292 430 937 292
Size: 138 507 4106
Fragments arranged by size:
4106 507 138
EcoO109I rG'GnC_Cy
Cuts at: 234 1093 1216 1331 1890 234
Size: 859 123 115 559 3095
Fragments arranged by size:
3095 859 559 123 115
EcoRI G'AATT_C
Cuts at: 1735 1755 1735
Size: 20 4731
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2770 2770
Size: 4751
FspI TGC'GCA
Cuts at: 2532 2532
Size: 4751
HaeII r_GCGC'y
Cuts at: 164 398 409 495 900 2185 2433 3455 4434
Size: 234 11 86 405 1285 248 1022 979
Cuts at: 4434 164
Size: 481
Fragments arranged by size:
1285 1022 979 481 405 248 234 86
11
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2593 2623 2625 2655 2593
Size: 30 2 30 4689
Fragments arranged by size:
4689 30 30 2
HincII GTy'rAC
Cuts at: 1790 1790
Size: 4751
KasI G'GCGC_C
Cuts at: 160 491 2181 2429 160
Size: 331 1690 248 2482
Fragments arranged by size:
2482 1690 331 248
KpnI G_GTAC'C
Cuts at: 1771 1771
Size: 4751
MluI A'CGCG_T
Cuts at: 4184 4184
Size: 4751
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 203 767 3422 3606 203
Size: 564 2655 184 1348
Fragments arranged by size:
2655 1348 564 184
MscI TGG'CCA
Cuts at: 1301 2512 1301
Size: 1211 3540
MslI CAynn'nnrTG
Cuts at: 1287 2740 2867 1287
Size: 1453 127 3171
Fragments arranged by size:
3171 1453 127
MspA1I CmG'CkG
Cuts at: 339 462 681 1237 1862 2176 2536 3549 3794
Size: 123 219 556 625 314 360 1013 245
Cuts at: 3794 339
Size: 1296
Fragments arranged by size:
1296 1013 625 556 360 314 245 219
123
NaeI GCC'GGC
Cuts at: 2932 2932
Size: 4751
NarI GG'CG_CC
Cuts at: 161 492 2182 2430 161
Size: 331 1690 248 2482
Fragments arranged by size:
2482 1690 331 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 4751
NgoMIV G'CCGG_C
Cuts at: 2930 2930
Size: 4751
NspI r_CATG'y
Cuts at: 1689 1804 2304 3211 1689
Size: 115 500 907 3229
Fragments arranged by size:
3229 907 500 115
PasI CC'CwG_GG
Cuts at: 271 571 1270 1699 271
Size: 300 699 429 3323
Fragments arranged by size:
3323 699 429 300
PciI A'CATG_T
Cuts at: 3207 3207
Size: 4751
PmeI GTTT'AAAC
Cuts at: 1750 1750
Size: 4751
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3890 3922 3890
Size: 32 4719
PpuMI rG'GwC_Cy
Cuts at: 234 234
Size: 4751
PspOMI G'GGCC_C
Cuts at: 1093 1331 1093
Size: 238 4513
PstI C_TGCA'G
Cuts at: 293 431 461 479 547 1423 1798 2483 293
Size: 138 30 18 68 876 375 685 2561
Fragments arranged by size:
2561 876 685 375 138 68 30 18
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4751
PvuII CAG'CTG
Cuts at: 462 681 1237 2176 2536 462
Size: 219 556 939 360 2677
Fragments arranged by size:
2677 939 556 360 219
RsrII CG'GwC_CG
Cuts at: 2946 2946
Size: 4751
SacI G_AGCT'C
Cuts at: 854 1765 854
Size: 911 3840
SalI G'TCGA_C
Cuts at: 1788 1788
Size: 4751
SapI GCTCTTCn'nnn_
Cuts at: 440 2774 2984 440
Size: 2334 210 2207
Fragments arranged by size:
2334 2207 210
SbfI CC_TGCA'GG
Cuts at: 293 431 1798 293
Size: 138 1367 3246
Fragments arranged by size:
3246 1367 138
ScaI AGT'ACT
Cuts at: 4268 4268
Size: 4751
SfcI C'TryA_G
Cuts at: 33 183 289 427 457 475 543 937 1419
Size: 150 106 138 30 18 68 394 482
Cuts at: 1419 1794 2479 3472 3663 33
Size: 375 685 993 191 1121
Fragments arranged by size:
1121 993 685 482 394 375 191 150
138 106 68 30 18
SfoI GGC'GCC
Cuts at: 162 493 2183 2431 162
Size: 331 1690 248 2482
Fragments arranged by size:
2482 1690 331 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4751
SmaI CCC'GGG
Cuts at: 1091 1716 1773 1091
Size: 625 57 4069
Fragments arranged by size:
4069 625 57
SmlI C'TyrA_G
Cuts at: 1906 2821 3313 3575 1906
Size: 915 492 262 3082
Fragments arranged by size:
3082 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4751
SphI G_CATG'C
Cuts at: 1804 2304 1804
Size: 500 4251
StuI AGG'CCT
Cuts at: 1109 1358 1531 1109
Size: 249 173 4329
Fragments arranged by size:
4329 249 173
StyI C'CwwG_G
Cuts at: 107 389 1885 2239 107
Size: 282 1496 354 2619
Fragments arranged by size:
2619 1496 354 282
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1422 2334 2467 2926 3135 1422
Size: 912 133 459 209 3038
Fragments arranged by size:
3038 912 459 209 133
TatI w'GTAC_w
Cuts at: 981 4266 981
Size: 3285 1466
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 181 213 1294 1326 1468 1500 4268 4300 181
Size: 32 1081 32 142 32 2768 32 632
Fragments arranged by size:
2768 1081 632 142 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 307 2548 307
Size: 2241 2510
XbaI T'CTAG_A
Cuts at: 1782 1782
Size: 4751
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 750 2246 750
Size: 1496 3255
XmaI C'CCGG_G
Cuts at: 1089 1714 1771 1089
Size: 625 57 4069
Fragments arranged by size:
4069 625 57
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PasI PciI
PmeI PpiI PpuMI PspOMI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AflII AhdI AleI AscI AvrII BfrBI BmtI
BsiWI BsmI BsmBI BsrGI BssHII BstZ17I CspCI DraIII
EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI
NotI NruI NsiI PacI PflMI PfoI PmlI PshAI
PsiI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI SspI SwaI XhoI XmnI ZraI