Restriction map for
pF1KB6112
(Circular) (Six-base) MAPSORT of: pF1KB6112.seq Check: 5572 from: 1 to: 4970
pF1KB6112 4970 bp
With 182 enzymes: SgfI *
October 7, 2009 14:19 ..
AatII G_ACGT'C
Cuts at: 1535 1535
Size: 4970
AccI GT'mk_AC
Cuts at: 1550 2008 1550
Size: 458 4512
Acc65I G'GTAC_C
Cuts at: 1986 1986
Size: 4970
AclI AA'CG_TT
Cuts at: 1490 4810 1490
Size: 3320 1650
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 739 1006 1072 1492 1751 1858 2795 3227 3974
Size: 267 66 420 259 107 937 432 747
Cuts at: 3974 4242 739
Size: 268 1467
Fragments arranged by size:
1467 937 747 432 420 268 267 259
107 66
AflIII A'CryG_T
Cuts at: 150 953 1480 2322 3426 4403 150
Size: 803 527 842 1104 977 717
Fragments arranged by size:
1104 977 842 803 717 527
AgeI A'CCGG_T
Cuts at: 3358 3358
Size: 4970
AhdI GACnn_n'nnGTC
Cuts at: 1620 1620
Size: 4970
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1710 1742 1710
Size: 32 4938
AlwNI CAG_nnn'CTG
Cuts at: 860 2275 3842 860
Size: 1415 1567 1988
Fragments arranged by size:
1988 1567 1415
ApaI G_GGCC'C
Cuts at: 585 585
Size: 4970
ApaLI G'TGCA_C
Cuts at: 3740 3740
Size: 4970
ApoI r'AATT_y
Cuts at: 1974 4323 4579 1974
Size: 2349 256 2365
Fragments arranged by size:
2365 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4970
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4970
AvaI C'yCGr_G
Cuts at: 311 1625 1852 1990 311
Size: 1314 227 138 3291
Fragments arranged by size:
3291 1314 227 138
BamHI G'GATC_C
Cuts at: 1995 1995
Size: 4970
BanI G'GyrC_C
Cuts at: 433 975 1785 1986 2400 2648 2683 433
Size: 542 810 201 414 248 35 2720
Fragments arranged by size:
2720 810 542 414 248 201 35
BanII G_rGCy'C
Cuts at: 411 585 1067 1325 1984 3014 411
Size: 174 482 258 659 1030 2367
Fragments arranged by size:
2367 1030 659 482 258 174
BbeI G_GCGC'C
Cuts at: 2404 2652 2404
Size: 248 4722
BbsI GAAGACnn'nnnn_
Cuts at: 245 605 1593 245
Size: 360 988 3622
Fragments arranged by size:
3622 988 360
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1833 1867 1833
Size: 34 4936
BciVI GTATCCnnnnn_n'
Cuts at: 2868 3629 4970 2868
Size: 761 1341 2868
Fragments arranged by size:
2868 1341 761
BclI T'GATC_A
Cuts at: 749 2490 749
Size: 1741 3229
BglI GCCn_nnn'nGGC
Cuts at: 1163 1163
Size: 4970
BglII A'GATC_T
Cuts at: 1900 2485 4522 1900
Size: 585 2037 2348
Fragments arranged by size:
2348 2037 585
BlpI GC'TnA_GC
Cuts at: 1401 2082 1401
Size: 681 4289
Bme1580I G_kGCm'C
Cuts at: 545 585 1790 2595 2688 3744 545
Size: 40 1205 805 93 1056 1771
Fragments arranged by size:
1771 1205 1056 805 93 40
BmrI ACTGGGnnnn_n'
Cuts at: 579 1830 1896 2182 2263 2598 4742 579
Size: 1251 66 286 81 335 2144 807
Fragments arranged by size:
2144 1251 807 335 286 81 66
BmtI G_CTAG'C
Cuts at: 1676 1676
Size: 4970
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3337 3337
Size: 4970
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 398 1328 1761 1784 398
Size: 930 433 23 3584
Fragments arranged by size:
3584 930 433 23
Bpu10I CC'TnA_GC
Cuts at: 514 902 1360 514
Size: 388 458 4124
Fragments arranged by size:
4124 458 388
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 283 942 1909 2146 3025 3517 3815 283
Size: 659 967 237 879 492 298 1438
Fragments arranged by size:
1438 967 879 659 492 298 237
BsaI GGTCTCn'nnnn_
Cuts at: 1097 1304 4674 1097
Size: 207 3370 1393
Fragments arranged by size:
3370 1393 207
BsaAI yAC'GTr
Cuts at: 2323 2323
Size: 4970
BsaBI GATnn'nnATC
Cuts at: 6 374 670 2484 4521 6
Size: 368 296 1814 2037 455
Fragments arranged by size:
2037 1814 455 368 296
BsaHI Gr'CG_yC
Cuts at: 602 1532 2401 2649 3351 4847 602
Size: 930 869 248 702 1496 725
Fragments arranged by size:
1496 930 869 725 702 248
BsaWI w'CCGG_w
Cuts at: 194 2159 2680 3358 3632 3779 4190 194
Size: 1965 521 678 274 147 411 974
Fragments arranged by size:
1965 974 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 757 787 1307 1337 1782 1812 757
Size: 30 520 30 445 30 3915
Fragments arranged by size:
3915 520 445 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 401 437 401
Size: 36 4934
BseYI C'CCAG_C
Cuts at: 1523 2395 3153 3730 4226 1523
Size: 872 758 577 496 2267
Fragments arranged by size:
2267 872 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 555 1745 555
Size: 1190 3780
BsiEI CG_ry'CG
Cuts at: 104 2512 2558 3766 104
Size: 2408 46 1208 1308
Fragments arranged by size:
2408 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 411 1067 1984 2762 2952 3744 411
Size: 656 917 778 190 792 1637
Fragments arranged by size:
1637 917 792 778 656 190
BsmI GAATG_Cn'
Cuts at: 380 1364 1725 1825 1954 380
Size: 984 361 100 129 3396
Fragments arranged by size:
3396 984 361 129 100
BsmBI CGTCTCn'nnnn_
Cuts at: 1489 1489
Size: 4970
Bsp1286I G_dGCh'C
Cuts at: 411 545 585 1067 1325 1790 1984 2595 2688
Size: 134 40 482 258 465 194 611 93
Cuts at: 2688 2762 2952 3014 3744 411
Size: 74 190 62 730 1637
Fragments arranged by size:
1637 730 611 482 465 258 194 190
134 93 74 62 40
BspEI T'CCGG_A
Cuts at: 4190 4190
Size: 4970
BspHI T'CATG_A
Cuts at: 752 1958 4146 752
Size: 1206 2188 1576
Fragments arranged by size:
2188 1576 1206
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2020 2234 2536 2917 2020
Size: 214 302 381 4073
Fragments arranged by size:
4073 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1026 3262 3316 4800 4965 50
Size: 976 2236 54 1484 165 55
Fragments arranged by size:
2236 1484 976 165 55 54
BsrDI GCAATG_nn'
Cuts at: 293 1573 1658 2882 293
Size: 1280 85 1224 2381
Fragments arranged by size:
2381 1280 1224 85
BsrFI r'CCGG_y
Cuts at: 2968 3149 3358 2968
Size: 181 209 4580
Fragments arranged by size:
4580 209 181
BsrGI T'GTAC_A
Cuts at: 938 938
Size: 4970
BssSI C'ACGA_G
Cuts at: 3241 3599 3241
Size: 358 4612
BstAPI GCAn_nnn'nTGC
Cuts at: 1278 2347 1278
Size: 1069 3901
BstBI TT'CG_AA
Cuts at: 3331 3331
Size: 4970
BstYI r'GATC_y
Cuts at: 1 989 1736 1900 1940 1995 2462 2485 2820
Size: 988 747 164 40 55 467 23 335
Cuts at: 2820 3066 4078 4164 4176 4522 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1012 988 747 467 449 346 335 246
164 86 55 40 23 12
Bsu36I CC'TnA_GG
Cuts at: 877 877
Size: 4970
BtgI C'CryG_G
Cuts at: 107 107
Size: 4970
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2920 3101 3264 2920
Size: 181 163 4626
Fragments arranged by size:
4626 181 163
BtsI GCAGTG_nn'
Cuts at: 543 2213 543
Size: 1670 3300
DraI TTT'AAA
Cuts at: 1969 1969
Size: 4970
DrdI GACnn_nn'nnGTC
Cuts at: 347 2676 3534 347
Size: 2329 858 1783
Fragments arranged by size:
2329 1783 858
EaeI y'GGCC_r
Cuts at: 1164 2555 2729 3120 3147 4492 1164
Size: 1391 174 391 27 1345 1642
Fragments arranged by size:
1642 1391 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2555 2555
Size: 4970
EarI CTCTTCn'nnn_
Cuts at: 225 690 884 1055 1252 1727 2993 3203 225
Size: 465 194 171 197 475 1266 210 1992
Fragments arranged by size:
1992 1266 475 465 210 197 194 171
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 127 3178 3488 3634 4780 127
Size: 3051 310 146 1146 317
Fragments arranged by size:
3051 1146 317 310 146
EcoICRI GAG'CTC
Cuts at: 409 1065 1982 409
Size: 656 917 3397
Fragments arranged by size:
3397 917 656
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 398 739 1006 1072 1328 1492 1751 1761 1784
Size: 341 267 66 256 164 259 10 23
Cuts at: 1784 1858 2795 3227 3974 4242 398
Size: 74 937 432 747 268 1126
Fragments arranged by size:
1126 937 747 432 341 268 267 259
256 164 74 66 23 10
EcoO109I rG'GnC_Cy
Cuts at: 187 449 1346 2109 187
Size: 262 897 763 3048
Fragments arranged by size:
3048 897 763 262
EcoRI G'AATT_C
Cuts at: 1974 1974
Size: 4970
EcoRV GAT'ATC
Cuts at: 668 668
Size: 4970
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2989 2989
Size: 4970
FspI TGC'GCA
Cuts at: 2751 2751
Size: 4970
HaeII r_GCGC'y
Cuts at: 1127 1562 2404 2652 3674 4653 1127
Size: 435 842 248 1022 979 1444
Fragments arranged by size:
1444 1022 979 842 435 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 109 141 436 468 633 665 895 927 940
Size: 32 295 32 165 32 230 32 13
Cuts at: 940 972 1066 1098 1307 1339 1606 1638 2812
Size: 32 94 32 209 32 267 32 1174
Cuts at: 2812 2842 2844 2874 109
Size: 30 2 30 2205
Fragments arranged by size:
2205 1174 295 267 230 209 165 94
32 32 32 32 32 32 32 32
30 30 13 2
HincII GTy'rAC
Cuts at: 633 2009 633
Size: 1376 3594
HindIII A'AGCT_T
Cuts at: 1438 1438
Size: 4970
KasI G'GCGC_C
Cuts at: 2400 2648 2400
Size: 248 4722
KpnI G_GTAC'C
Cuts at: 1990 1990
Size: 4970
MfeI C'AATT_G
Cuts at: 1184 1184
Size: 4970
MluI A'CGCG_T
Cuts at: 150 1480 4403 150
Size: 1330 2923 717
Fragments arranged by size:
2923 1330 717
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3641 3825 3641
Size: 184 4786
MscI TGG'CCA
Cuts at: 1166 2731 1166
Size: 1565 3405
MslI CAynn'nnrTG
Cuts at: 143 1578 2959 3086 143
Size: 1435 1381 127 2027
Fragments arranged by size:
2027 1435 1381 127
MspA1I CmG'CkG
Cuts at: 2081 2395 2755 3768 4013 2081
Size: 314 360 1013 245 3038
Fragments arranged by size:
3038 1013 360 314 245
NaeI GCC'GGC
Cuts at: 3151 3151
Size: 4970
NarI GG'CG_CC
Cuts at: 2401 2649 2401
Size: 248 4722
NcoI C'CATG_G
Cuts at: 107 107
Size: 4970
NgoMIV G'CCGG_C
Cuts at: 3149 3149
Size: 4970
NheI G'CTAG_C
Cuts at: 1672 1672
Size: 4970
NruI TCG'CGA
Cuts at: 1133 1133
Size: 4970
NspI r_CATG'y
Cuts at: 957 2023 2523 3430 957
Size: 1066 500 907 2497
Fragments arranged by size:
2497 1066 907 500
PciI A'CATG_T
Cuts at: 953 3426 953
Size: 2473 2497
PfoI T'CCnGG_A
Cuts at: 1325 1325
Size: 4970
PmeI GTTT'AAAC
Cuts at: 1969 1969
Size: 4970
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4109 4141 4109
Size: 32 4938
PpuMI rG'GwC_Cy
Cuts at: 187 449 187
Size: 262 4708
PspOMI G'GGCC_C
Cuts at: 581 581
Size: 4970
PspXI vC'TCGA_Gb
Cuts at: 1852 1852
Size: 4970
PstI C_TGCA'G
Cuts at: 2017 2702 2017
Size: 685 4285
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4970
PvuII CAG'CTG
Cuts at: 2395 2755 2395
Size: 360 4610
RsrII CG'GwC_CG
Cuts at: 3165 3165
Size: 4970
SacI G_AGCT'C
Cuts at: 411 1067 1984 411
Size: 656 917 3397
Fragments arranged by size:
3397 917 656
SalI G'TCGA_C
Cuts at: 2007 2007
Size: 4970
SanDI GG'GwC_CC
Cuts at: 187 187
Size: 4970
SapI GCTCTTCn'nnn_
Cuts at: 1055 2993 3203 1055
Size: 1938 210 2822
Fragments arranged by size:
2822 1938 210
SbfI CC_TGCA'GG
Cuts at: 2017 2017
Size: 4970
ScaI AGT'ACT
Cuts at: 609 1288 4487 609
Size: 679 3199 1092
Fragments arranged by size:
3199 1092 679
SfcI C'TryA_G
Cuts at: 33 2013 2698 3691 3882 33
Size: 1980 685 993 191 1121
Fragments arranged by size:
1980 1121 993 685 191
SfoI GGC'GCC
Cuts at: 2402 2650 2402
Size: 248 4722
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4970
SmaI CCC'GGG
Cuts at: 1627 1992 1627
Size: 365 4605
SmlI C'TyrA_G
Cuts at: 262 921 1852 1924 2125 3040 3532 3794 262
Size: 659 931 72 201 915 492 262 1438
Fragments arranged by size:
1438 931 915 659 492 262 201 72
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4970
SphI G_CATG'C
Cuts at: 2023 2523 2023
Size: 500 4470
StuI AGG'CCT
Cuts at: 800 1912 800
Size: 1112 3858
StyI C'CwwG_G
Cuts at: 107 1931 2104 2458 107
Size: 1824 173 354 2619
Fragments arranged by size:
2619 1824 354 173
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 239 993 1328 1779 2553 2686 3145 3354 239
Size: 754 335 451 774 133 459 209 1855
Fragments arranged by size:
1855 774 754 459 451 335 209 133
TatI w'GTAC_w
Cuts at: 607 938 1286 4485 607
Size: 331 348 3199 1092
Fragments arranged by size:
3199 1092 348 331
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 192 224 1782 1814 4487 4519 192
Size: 32 1558 32 2673 32 643
Fragments arranged by size:
2673 1558 643 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 841 2767 841
Size: 1926 3044
XbaI T'CTAG_A
Cuts at: 2001 2001
Size: 4970
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2465 2465
Size: 4970
XhoI C'TCGA_G
Cuts at: 1852 1852
Size: 4970
XmaI C'CCGG_G
Cuts at: 1625 1990 1625
Size: 365 4605
XmnI GAAnn'nnTTC
Cuts at: 463 1139 463
Size: 676 4294
ZraI GAC'GTC
Cuts at: 1533 1533
Size: 4970
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AgeI AhdI
AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI
BstAPI BstBI BstYI Bsu36I BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NheI NruI NspI PciI
PfoI PmeI PpiI PpuMI PspOMI PspXI PstI PvuI
PvuII RsrII SacI SalI SanDI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AflII AleI AlfI AscI AvrII BaeI
BbvCI BfrBI BmgBI BsiWI BssHII BstEII BstXI BstZ17I
ClaI CspCI DraIII EcoNI FseI FspAI HpaI NdeI
NotI NsiI PacI PasI PflMI PmlI PshAI PsiI
PsrI SacII SexAI SfiI SgrAI SnaBI SrfI SspI
SwaI