Restriction map for
pF1KB6110
(Circular) (Six-base) MAPSORT of: pF1KB6110.seq Check: 5486 from: 1 to: 4997
pF1KB6110 4997 bp
With 182 enzymes: SgfI *
October 7, 2009 14:19 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1141 1151 1668 1141
Size: 10 517 4470
Fragments arranged by size:
4470 517 10
AccI GT'mk_AC
Cuts at: 2035 2035
Size: 4997
Acc65I G'GTAC_C
Cuts at: 2013 2013
Size: 4997
AclI AA'CG_TT
Cuts at: 4837 4837
Size: 4997
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 262 1696 2822 3254 4001 4269 262
Size: 1434 1126 432 747 268 990
Fragments arranged by size:
1434 1126 990 747 432 268
AflIII A'CryG_T
Cuts at: 1201 2349 3453 4430 1201
Size: 1148 1104 977 1768
Fragments arranged by size:
1768 1148 1104 977
AgeI A'CCGG_T
Cuts at: 3385 3385
Size: 4997
AhdI GACnn_n'nnGTC
Cuts at: 1721 1964 1721
Size: 243 4754
AleI CACnn'nnGTG
Cuts at: 880 1083 880
Size: 203 4794
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 965 965
Size: 4997
AlwNI CAG_nnn'CTG
Cuts at: 865 2302 3869 865
Size: 1437 1567 1993
Fragments arranged by size:
1993 1567 1437
ApaI G_GGCC'C
Cuts at: 323 433 1822 323
Size: 110 1389 3498
Fragments arranged by size:
3498 1389 110
ApaLI G'TGCA_C
Cuts at: 475 3767 475
Size: 3292 1705
ApoI r'AATT_y
Cuts at: 2001 4350 4606 2001
Size: 2349 256 2392
Fragments arranged by size:
2392 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4997
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4997
AvaI C'yCGr_G
Cuts at: 2017 2017
Size: 4997
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1636 1669 1636
Size: 33 4964
BamHI G'GATC_C
Cuts at: 1315 2022 1315
Size: 707 4290
BanI G'GyrC_C
Cuts at: 355 418 1322 1866 2013 2427 2675 2710 355
Size: 63 904 544 147 414 248 35 2642
Fragments arranged by size:
2642 904 544 414 248 147 63 35
BanII G_rGCy'C
Cuts at: 164 218 323 433 530 812 1600 1822 1846
Size: 54 105 110 97 282 788 222 24
Cuts at: 1846 2011 3041 164
Size: 165 1030 2120
Fragments arranged by size:
2120 1030 788 282 222 165 110 105
97 54 24
BbeI G_GCGC'C
Cuts at: 1870 2431 2679 1870
Size: 561 248 4188
Fragments arranged by size:
4188 561 248
BbsI GAAGACnn'nnnn_
Cuts at: 316 316
Size: 4997
BbvCI CC'TCA_GC
Cuts at: 1521 1521
Size: 4997
BciVI GTATCCnnnnn_n'
Cuts at: 997 2895 3656 4997 997
Size: 1898 761 1341 997
Fragments arranged by size:
1898 1341 997 761
BclI T'GATC_A
Cuts at: 1016 2517 1016
Size: 1501 3496
BglI GCCn_nnn'nGGC
Cuts at: 816 1534 1864 816
Size: 718 330 3949
Fragments arranged by size:
3949 718 330
BglII A'GATC_T
Cuts at: 2512 4549 2512
Size: 2037 2960
BlpI GC'TnA_GC
Cuts at: 2109 2109
Size: 4997
Bme1580I G_kGCm'C
Cuts at: 258 323 433 479 769 1014 1090 1128 1327
Size: 65 110 46 290 245 76 38 199
Cuts at: 1327 1612 1822 2622 2715 3771 258
Size: 285 210 800 93 1056 1484
Fragments arranged by size:
1484 1056 800 290 285 245 210 199
110 93 76 65 46 38
BmrI ACTGGGnnnn_n'
Cuts at: 1396 1681 2209 2290 2625 4769 1396
Size: 285 528 81 335 2144 1624
Fragments arranged by size:
2144 1624 528 335 285 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3364 3364
Size: 4997
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 148 1375 148
Size: 1227 3770
Bpu10I CC'TnA_GC
Cuts at: 682 1521 1591 682
Size: 839 70 4088
Fragments arranged by size:
4088 839 70
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1935 2173 3052 3544 3842 1935
Size: 238 879 492 298 3090
Fragments arranged by size:
3090 879 492 298 238
BsaI GGTCTCn'nnnn_
Cuts at: 694 4701 694
Size: 4007 990
BsaAI yAC'GTr
Cuts at: 2350 2350
Size: 4997
BsaBI GATnn'nnATC
Cuts at: 6 274 2511 4548 6
Size: 268 2237 2037 455
Fragments arranged by size:
2237 2037 455 268
BsaHI Gr'CG_yC
Cuts at: 1867 2428 2676 3378 4874 1867
Size: 561 248 702 1496 1990
Fragments arranged by size:
1990 1496 702 561 248
BsaWI w'CCGG_w
Cuts at: 989 2186 2707 3385 3659 3806 4217 989
Size: 1197 521 678 274 147 411 1769
Fragments arranged by size:
1769 1197 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1495 1495
Size: 4997
BseYI C'CCAG_C
Cuts at: 117 426 467 605 820 1836 2422 3180 3757
Size: 309 41 138 215 1016 586 758 577
Cuts at: 3757 4253 117
Size: 496 861
Fragments arranged by size:
1016 861 758 586 577 496 309 215
138 41
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 217 634 697 217
Size: 417 63 4517
Fragments arranged by size:
4517 417 63
BsiEI CG_ry'CG
Cuts at: 104 2539 2585 3793 104
Size: 2435 46 1208 1308
Fragments arranged by size:
2435 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 164 218 479 530 1846 2011 2789 2979 3771
Size: 54 261 51 1316 165 778 190 792
Cuts at: 3771 164
Size: 1390
Fragments arranged by size:
1390 1316 792 778 261 190 165 54
51
Bsp1286I G_dGCh'C
Cuts at: 164 218 258 323 433 479 530 769 812
Size: 54 40 65 110 46 51 239 43
Cuts at: 812 1014 1090 1128 1327 1600 1612 1822 1846
Size: 202 76 38 199 273 12 210 24
Cuts at: 1846 2011 2622 2715 2789 2979 3041 3771 164
Size: 165 611 93 74 190 62 730 1390
Fragments arranged by size:
1390 730 611 273 239 210 202 199
190 165 110 93 76 74 65 62
54 51 46 43 40 38 24 12
BspEI T'CCGG_A
Cuts at: 989 4217 989
Size: 3228 1769
BspHI T'CATG_A
Cuts at: 4173 4173
Size: 4997
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1141 1151 1668 2047 2261 2563 2944 1141
Size: 10 517 379 214 302 381 3194
Fragments arranged by size:
3194 517 381 379 302 214 10
BsrBI CCG'CTC
Cuts at: 50 3289 3343 4827 4992 50
Size: 3239 54 1484 165 55
Fragments arranged by size:
3239 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 499 2909 499
Size: 2410 2587
BsrFI r'CCGG_y
Cuts at: 2995 3176 3385 2995
Size: 181 209 4607
Fragments arranged by size:
4607 209 181
BssSI C'ACGA_G
Cuts at: 211 3268 3626 211
Size: 3057 358 1582
Fragments arranged by size:
3057 1582 358
BstAPI GCAn_nnn'nTGC
Cuts at: 2374 2374
Size: 4997
BstBI TT'CG_AA
Cuts at: 3358 3358
Size: 4997
BstEII G'GTnAC_C
Cuts at: 778 778
Size: 4997
BstYI r'GATC_y
Cuts at: 1 1295 1315 1416 1485 2022 2489 2512 2847
Size: 1294 20 101 69 537 467 23 335
Cuts at: 2847 3093 4105 4191 4203 4549 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1294 1012 537 467 449 346 335 246
101 86 69 23 20 12
Bsu36I CC'TnA_GG
Cuts at: 1944 1944
Size: 4997
BtgI C'CryG_G
Cuts at: 107 107
Size: 4997
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2947 3128 3291 2947
Size: 181 163 4653
Fragments arranged by size:
4653 181 163
BtsI GCAGTG_nn'
Cuts at: 1848 2240 1848
Size: 392 4605
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 528 563 528
Size: 35 4962
DraI TTT'AAA
Cuts at: 1996 1996
Size: 4997
DraIII CAC_nnn'GTG
Cuts at: 138 138
Size: 4997
DrdI GACnn_nn'nnGTC
Cuts at: 2703 3561 2703
Size: 858 4139
EaeI y'GGCC_r
Cuts at: 186 221 1055 1569 1776 1878 2582 2756 3147
Size: 35 834 514 207 102 704 174 391
Cuts at: 3147 3174 4519 186
Size: 27 1345 664
Fragments arranged by size:
1345 834 704 664 514 391 207 174
102 35 27
EagI C'GGCC_G
Cuts at: 2582 2582
Size: 4997
EarI CTCTTCn'nnn_
Cuts at: 3020 3230 3020
Size: 210 4787
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 127 3205 3515 3661 4807 127
Size: 3078 310 146 1146 317
Fragments arranged by size:
3078 1146 317 310 146
EcoICRI GAG'CTC
Cuts at: 162 216 528 1844 2009 162
Size: 54 312 1316 165 3150
Fragments arranged by size:
3150 1316 312 165 54
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 148 262 1375 1696 2822 3254 4001 4269 148
Size: 114 1113 321 1126 432 747 268 876
Fragments arranged by size:
1126 1113 876 747 432 321 268 114
EcoNI CCTnn'n_nnAGG
Cuts at: 223 223
Size: 4997
EcoO109I rG'GnC_Cy
Cuts at: 319 320 430 1799 1910 1933 1948 2136 319
Size: 1 110 1369 111 23 15 188 3180
Fragments arranged by size:
3180 1369 188 111 110 23 15 1
EcoRI G'AATT_C
Cuts at: 2001 2001
Size: 4997
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3016 3016
Size: 4997
FspI TGC'GCA
Cuts at: 2778 2778
Size: 4997
HaeII r_GCGC'y
Cuts at: 1859 1870 2431 2679 3701 4680 1859
Size: 11 561 248 1022 979 2176
Fragments arranged by size:
2176 1022 979 561 248 11
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1615 1647 1667 1699 1707 1739 1791 1823 1950
Size: 32 20 32 8 32 52 32 127
Cuts at: 1950 1982 2839 2869 2871 2901 1615
Size: 32 857 30 2 30 3711
Fragments arranged by size:
3711 857 127 52 32 32 32 32
32 30 30 20 8 2
HincII GTy'rAC
Cuts at: 947 2036 947
Size: 1089 3908
KasI G'GCGC_C
Cuts at: 1866 2427 2675 1866
Size: 561 248 4188
Fragments arranged by size:
4188 561 248
KpnI G_GTAC'C
Cuts at: 2017 2017
Size: 4997
MfeI C'AATT_G
Cuts at: 888 888
Size: 4997
MluI A'CGCG_T
Cuts at: 4430 4430
Size: 4997
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3668 3852 3668
Size: 184 4813
MscI TGG'CCA
Cuts at: 223 1057 1571 1778 2758 223
Size: 834 514 207 980 2462
Fragments arranged by size:
2462 980 834 514 207
MslI CAynn'nnrTG
Cuts at: 139 482 673 880 1083 1200 2986 3113 139
Size: 343 191 207 203 117 1786 127 2023
Fragments arranged by size:
2023 1786 343 207 203 191 127 117
MspA1I CmG'CkG
Cuts at: 251 567 707 1525 2108 2422 2782 3795 4040
Size: 316 140 818 583 314 360 1013 245
Cuts at: 4040 251
Size: 1208
Fragments arranged by size:
1208 1013 818 583 360 316 314 245
140
NaeI GCC'GGC
Cuts at: 3178 3178
Size: 4997
NarI GG'CG_CC
Cuts at: 1867 2428 2676 1867
Size: 561 248 4188
Fragments arranged by size:
4188 561 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 4997
NgoMIV G'CCGG_C
Cuts at: 3176 3176
Size: 4997
NspI r_CATG'y
Cuts at: 453 1205 2050 2550 3457 453
Size: 752 845 500 907 1993
Fragments arranged by size:
1993 907 845 752 500
PasI CC'CwG_GG
Cuts at: 315 1903 315
Size: 1588 3409
PciI A'CATG_T
Cuts at: 1201 3453 1201
Size: 2252 2745
PflMI CCAn_nnn'nTGG
Cuts at: 1305 1375 1305
Size: 70 4927
PmeI GTTT'AAAC
Cuts at: 1996 1996
Size: 4997
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4136 4168 4136
Size: 32 4965
PpuMI rG'GwC_Cy
Cuts at: 1799 1933 1948 1799
Size: 134 15 4848
Fragments arranged by size:
4848 134 15
PspOMI G'GGCC_C
Cuts at: 319 429 1818 319
Size: 110 1389 3498
Fragments arranged by size:
3498 1389 110
PstI C_TGCA'G
Cuts at: 954 1148 1855 2044 2729 954
Size: 194 707 189 685 3222
Fragments arranged by size:
3222 707 685 194 189
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4997
PvuII CAG'CTG
Cuts at: 251 707 1525 2422 2782 251
Size: 456 818 897 360 2466
Fragments arranged by size:
2466 897 818 456 360
RsrII CG'GwC_CG
Cuts at: 3192 3192
Size: 4997
SacI G_AGCT'C
Cuts at: 164 218 530 1846 2011 164
Size: 54 312 1316 165 3150
Fragments arranged by size:
3150 1316 312 165 54
SalI G'TCGA_C
Cuts at: 2034 2034
Size: 4997
SanDI GG'GwC_CC
Cuts at: 1799 1799
Size: 4997
SapI GCTCTTCn'nnn_
Cuts at: 3020 3230 3020
Size: 210 4787
SbfI CC_TGCA'GG
Cuts at: 2044 2044
Size: 4997
ScaI AGT'ACT
Cuts at: 4514 4514
Size: 4997
SexAI A'CCwGG_T
Cuts at: 932 932
Size: 4997
SfcI C'TryA_G
Cuts at: 33 365 950 1144 1171 1851 2040 2725 3718
Size: 332 585 194 27 680 189 685 993
Cuts at: 3718 3909 33
Size: 191 1121
Fragments arranged by size:
1121 993 685 680 585 332 194 191
189 27
SfoI GGC'GCC
Cuts at: 1868 2429 2677 1868
Size: 561 248 4188
Fragments arranged by size:
4188 561 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4997
SmaI CCC'GGG
Cuts at: 2019 2019
Size: 4997
SmlI C'TyrA_G
Cuts at: 1914 2152 3067 3559 3821 1914
Size: 238 915 492 262 3090
Fragments arranged by size:
3090 915 492 262 238
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4997
SphI G_CATG'C
Cuts at: 2050 2550 2050
Size: 500 4497
StyI C'CwwG_G
Cuts at: 107 224 1686 1773 2131 2485 107
Size: 117 1462 87 358 354 2619
Fragments arranged by size:
2619 1462 358 354 117 87
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1606 1958 2580 2713 3172 3381 1606
Size: 352 622 133 459 209 3222
Fragments arranged by size:
3222 622 459 352 209 133
TatI w'GTAC_w
Cuts at: 1394 4512 1394
Size: 3118 1879
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 183 215 1564 1596 1649 1681 4514 4546 183
Size: 32 1349 32 53 32 2833 32 634
Fragments arranged by size:
2833 1349 634 53 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2794 2794
Size: 4997
XbaI T'CTAG_A
Cuts at: 2028 2028
Size: 4997
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 114 172 441 879 2492 114
Size: 58 269 438 1613 2619
Fragments arranged by size:
2619 1613 438 269 58
XmaI C'CCGG_G
Cuts at: 2017 2017
Size: 4997
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AhdI
AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI
BciVI BclI BglI BglII BlpI Bme1580I BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII
BstYI Bsu36I BtgI BtgZI BtsI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PasI PciI PflMI PmeI
PpiI PpuMI PspOMI PstI PvuI PvuII RsrII SacI
SalI SanDI SapI SbfI ScaI SexAI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AloI AscI AvrII BcgI BfrBI
BmgBI BmtI BsaXI BsiWI BsmI BsmBI BsrGI BssHII
BstXI BstZ17I ClaI EcoRV FseI FspAI HindIII HpaI
NdeI NheI NotI NruI NsiI PacI PfoI PmlI
PshAI PsiI PspXI PsrI SacII SfiI SgrAI SnaBI
SrfI SspI StuI SwaI XhoI XmnI ZraI