Restriction map for
pF1KB6107
(Circular) (Six-base) MAPSORT of: pF1KB6107.seq Check: 3222 from: 1 to: 4853
pF1KB6107 4853 bp
With 182 enzymes: SgfI *
October 7, 2009 14:19 ..
AccI GT'mk_AC
Cuts at: 1445 1891 1445
Size: 446 4407
Acc65I G'GTAC_C
Cuts at: 725 1869 725
Size: 1144 3709
AclI AA'CG_TT
Cuts at: 4693 4693
Size: 4853
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 607 1613 2678 3110 3857 4125 607
Size: 1006 1065 432 747 268 1335
Fragments arranged by size:
1335 1065 1006 747 432 268
AflIII A'CryG_T
Cuts at: 2205 3309 4286 2205
Size: 1104 977 2772
Fragments arranged by size:
2772 1104 977
AgeI A'CCGG_T
Cuts at: 749 815 3241 749
Size: 66 2426 2361
Fragments arranged by size:
2426 2361 66
AhdI GACnn_n'nnGTC
Cuts at: 216 216
Size: 4853
AleI CACnn'nnGTG
Cuts at: 1021 1021
Size: 4853
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1183 1215 1183
Size: 32 4821
AlwNI CAG_nnn'CTG
Cuts at: 522 1818 2158 3725 522
Size: 1296 340 1567 1650
Fragments arranged by size:
1650 1567 1296 340
ApaI G_GGCC'C
Cuts at: 183 716 856 183
Size: 533 140 4180
Fragments arranged by size:
4180 533 140
ApaLI G'TGCA_C
Cuts at: 3623 3623
Size: 4853
ApoI r'AATT_y
Cuts at: 1252 1394 1857 4206 4462 1252
Size: 142 463 2349 256 1643
Fragments arranged by size:
2349 1643 463 256 142
AseI AT'TA_AT
Cuts at: 20 20
Size: 4853
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4853
AvaI C'yCGr_G
Cuts at: 137 277 646 655 1636 1873 137
Size: 140 369 9 981 237 3117
Fragments arranged by size:
3117 981 369 237 140 9
AvrII C'CTAG_G
Cuts at: 414 414
Size: 4853
BamHI G'GATC_C
Cuts at: 126 1878 126
Size: 1752 3101
BanI G'GyrC_C
Cuts at: 151 595 725 1869 2283 2531 2566 151
Size: 444 130 1144 414 248 35 2438
Fragments arranged by size:
2438 1144 444 414 248 130 35
BanII G_rGCy'C
Cuts at: 123 183 398 656 716 800 856 992 1867
Size: 60 215 258 60 84 56 136 875
Cuts at: 1867 2897 123
Size: 1030 2079
Fragments arranged by size:
2079 1030 875 258 215 136 84 60
60 56
BbeI G_GCGC'C
Cuts at: 599 2287 2535 599
Size: 1688 248 2917
Fragments arranged by size:
2917 1688 248
BbsI GAAGACnn'nnnn_
Cuts at: 427 427
Size: 4853
BbvCI CC'TCA_GC
Cuts at: 1338 1338
Size: 4853
BciVI GTATCCnnnnn_n'
Cuts at: 2751 3512 4853 2751
Size: 761 1341 2751
Fragments arranged by size:
2751 1341 761
BclI T'GATC_A
Cuts at: 2373 2373
Size: 4853
BglII A'GATC_T
Cuts at: 2368 4405 2368
Size: 2037 2816
BlpI GC'TnA_GC
Cuts at: 1583 1965 1583
Size: 382 4471
Bme1580I G_kGCm'C
Cuts at: 183 716 856 2478 2571 3627 183
Size: 533 140 1622 93 1056 1409
Fragments arranged by size:
1622 1409 1056 533 140 93
BmrI ACTGGGnnnn_n'
Cuts at: 357 777 993 1015 1316 2065 2146 2481 4625
Size: 420 216 22 301 749 81 335 2144
Cuts at: 4625 357
Size: 585
Fragments arranged by size:
2144 749 585 420 335 301 216 81
22
BmtI G_CTAG'C
Cuts at: 1370 1370
Size: 4853
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 681 3220 681
Size: 2539 2314
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1365 1488 1365
Size: 123 4730
Bpu10I CC'TnA_GC
Cuts at: 1338 1419 1338
Size: 81 4772
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1696 2029 2908 3400 3698 1696
Size: 333 879 492 298 2851
Fragments arranged by size:
2851 879 492 333 298
BsaI GGTCTCn'nnnn_
Cuts at: 806 4557 806
Size: 3751 1102
BsaAI yAC'GTr
Cuts at: 2206 2206
Size: 4853
BsaBI GATnn'nnATC
Cuts at: 6 448 2367 4404 6
Size: 442 1919 2037 455
Fragments arranged by size:
2037 1919 455 442
BsaHI Gr'CG_yC
Cuts at: 596 2284 2532 3234 4730 596
Size: 1688 248 702 1496 719
Fragments arranged by size:
1688 1496 719 702 248
BsaWI w'CCGG_w
Cuts at: 749 815 2042 2563 3241 3515 3662 4073 749
Size: 66 1227 521 678 274 147 411 1529
Fragments arranged by size:
1529 1227 678 521 411 274 147 66
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 223 253 385 415 643 673 1028 1058 1183
Size: 30 132 30 228 30 355 30 125
Cuts at: 1183 1213 223
Size: 30 3863
Fragments arranged by size:
3863 355 228 132 125 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 406 664 970 1036 1191 406
Size: 258 306 66 155 4068
Fragments arranged by size:
4068 306 258 155 66
BseYI C'CCAG_C
Cuts at: 1744 2278 3036 3613 4109 1744
Size: 534 758 577 496 2488
Fragments arranged by size:
2488 758 577 534 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 762 774 1637 1760 762
Size: 12 863 123 3855
Fragments arranged by size:
3855 863 123 12
BsiEI CG_ry'CG
Cuts at: 104 2395 2441 3649 104
Size: 2291 46 1208 1308
Fragments arranged by size:
2291 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 123 656 992 1351 1867 2645 2835 3627 123
Size: 533 336 359 516 778 190 792 1349
Fragments arranged by size:
1349 792 778 533 516 359 336 190
Bsp1286I G_dGCh'C
Cuts at: 123 183 398 656 716 800 856 992 1351
Size: 60 215 258 60 84 56 136 359
Cuts at: 1351 1867 2478 2571 2645 2835 2897 3627 123
Size: 516 611 93 74 190 62 730 1349
Fragments arranged by size:
1349 730 611 516 359 258 215 190
136 93 84 74 62 60 60 56
BspEI T'CCGG_A
Cuts at: 4073 4073
Size: 4853
BspHI T'CATG_A
Cuts at: 4029 4029
Size: 4853
BspMI ACCTGCnnnn'nnnn_
Cuts at: 737 1903 2117 2419 2800 737
Size: 1166 214 302 381 2790
Fragments arranged by size:
2790 1166 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1641 3145 3199 4683 4848 50
Size: 1591 1504 54 1484 165 55
Fragments arranged by size:
1591 1504 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 697 748 2765 697
Size: 51 2017 2785
Fragments arranged by size:
2785 2017 51
BsrFI r'CCGG_y
Cuts at: 251 376 749 815 1073 2851 3032 3241 251
Size: 125 373 66 258 1778 181 209 1863
Fragments arranged by size:
1863 1778 373 258 209 181 125 66
BssHII G'CGCG_C
Cuts at: 581 581
Size: 4853
BssSI C'ACGA_G
Cuts at: 319 769 3124 3482 319
Size: 450 2355 358 1690
Fragments arranged by size:
2355 1690 450 358
BstAPI GCAn_nnn'nTGC
Cuts at: 410 785 2230 410
Size: 375 1445 3033
Fragments arranged by size:
3033 1445 375
BstBI TT'CG_AA
Cuts at: 329 3214 329
Size: 2885 1968
BstEII G'GTnAC_C
Cuts at: 472 472
Size: 4853
BstYI r'GATC_y
Cuts at: 1 126 443 1878 2345 2368 2703 2949 3961
Size: 125 317 1435 467 23 335 246 1012
Cuts at: 3961 4047 4059 4405 1
Size: 86 12 346 449
Fragments arranged by size:
1435 1012 467 449 346 335 317 246
125 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1799 1799
Size: 4853
BtgI C'CryG_G
Cuts at: 107 107
Size: 4853
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 585 658 897 2803 2984 3147 585
Size: 73 239 1906 181 163 2291
Fragments arranged by size:
2291 1906 239 181 163 73
BtsI GCAGTG_nn'
Cuts at: 490 513 766 1620 2096 490
Size: 23 253 854 476 3247
Fragments arranged by size:
3247 854 476 253 23
DraI TTT'AAA
Cuts at: 1406 1852 1406
Size: 446 4407
DraIII CAC_nnn'GTG
Cuts at: 785 785
Size: 4853
DrdI GACnn_nn'nnGTC
Cuts at: 2559 3417 2559
Size: 858 3995
EaeI y'GGCC_r
Cuts at: 1642 2438 2612 3003 3030 4375 1642
Size: 796 174 391 27 1345 2120
Fragments arranged by size:
2120 1345 796 391 174 27
EagI C'GGCC_G
Cuts at: 2438 2438
Size: 4853
EarI CTCTTCn'nnn_
Cuts at: 2876 3086 2876
Size: 210 4643
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1108 3061 3371 3517 4663 1108
Size: 1953 310 146 1146 1298
Fragments arranged by size:
1953 1298 1146 310 146
EcoICRI GAG'CTC
Cuts at: 121 654 990 1865 121
Size: 533 336 875 3109
Fragments arranged by size:
3109 875 533 336
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 607 1365 1488 1613 2678 3110 3857 4125 607
Size: 758 123 125 1065 432 747 268 1335
Fragments arranged by size:
1335 1065 758 747 432 268 125 123
EcoNI CCTnn'n_nnAGG
Cuts at: 187 358 1169 187
Size: 171 811 3871
Fragments arranged by size:
3871 811 171
EcoO109I rG'GnC_Cy
Cuts at: 179 180 351 852 1992 179
Size: 1 171 501 1140 3040
Fragments arranged by size:
3040 1140 501 171 1
EcoRI G'AATT_C
Cuts at: 1252 1857 1252
Size: 605 4248
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 219 960 1131 2872 219
Size: 741 171 1741 2200
Fragments arranged by size:
2200 1741 741 171
FspI TGC'GCA
Cuts at: 1126 2634 1126
Size: 1508 3345
HaeII r_GCGC'y
Cuts at: 599 1199 2287 2535 3557 4536 599
Size: 600 1088 248 1022 979 916
Fragments arranged by size:
1088 1022 979 916 600 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 202 234 536 568 1028 1060 1321 1353 1432
Size: 32 302 32 460 32 261 32 79
Cuts at: 1432 1464 1681 1713 2695 2725 2727 2757 202
Size: 32 217 32 982 30 2 30 2298
Fragments arranged by size:
2298 982 460 302 261 217 79 32
32 32 32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 975 1446 1892 975
Size: 471 446 3936
Fragments arranged by size:
3936 471 446
KasI G'GCGC_C
Cuts at: 595 2283 2531 595
Size: 1688 248 2917
Fragments arranged by size:
2917 1688 248
KpnI G_GTAC'C
Cuts at: 729 1873 729
Size: 1144 3709
MluI A'CGCG_T
Cuts at: 4286 4286
Size: 4853
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3524 3708 3524
Size: 184 4669
MscI TGG'CCA
Cuts at: 2614 2614
Size: 4853
MslI CAynn'nnrTG
Cuts at: 377 1021 2842 2969 377
Size: 644 1821 127 2261
Fragments arranged by size:
2261 1821 644 127
MspA1I CmG'CkG
Cuts at: 435 1582 1744 1964 2278 2638 3651 3896 435
Size: 1147 162 220 314 360 1013 245 1392
Fragments arranged by size:
1392 1147 1013 360 314 245 220 162
NaeI GCC'GGC
Cuts at: 3034 3034
Size: 4853
NarI GG'CG_CC
Cuts at: 596 2284 2532 596
Size: 1688 248 2917
Fragments arranged by size:
2917 1688 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 4853
NgoMIV G'CCGG_C
Cuts at: 3032 3032
Size: 4853
NheI G'CTAG_C
Cuts at: 1366 1366
Size: 4853
NspI r_CATG'y
Cuts at: 376 1557 1906 2406 3313 376
Size: 1181 349 500 907 1916
Fragments arranged by size:
1916 1181 907 500 349
PciI A'CATG_T
Cuts at: 3309 3309
Size: 4853
PflMI CCAn_nnn'nTGG
Cuts at: 347 1362 1779 347
Size: 1015 417 3421
Fragments arranged by size:
3421 1015 417
PfoI T'CCnGG_A
Cuts at: 384 384
Size: 4853
PmeI GTTT'AAAC
Cuts at: 1852 1852
Size: 4853
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1183 1215 3992 4024 1183
Size: 32 2777 32 2012
Fragments arranged by size:
2777 2012 32 32
PpuMI rG'GwC_Cy
Cuts at: 351 351
Size: 4853
PsiI TTA'TAA
Cuts at: 1067 1067
Size: 4853
PspOMI G'GGCC_C
Cuts at: 179 712 852 179
Size: 533 140 4180
Fragments arranged by size:
4180 533 140
PspXI vC'TCGA_Gb
Cuts at: 655 655
Size: 4853
PstI C_TGCA'G
Cuts at: 617 998 1900 2585 617
Size: 381 902 685 2885
Fragments arranged by size:
2885 902 685 381
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4853
PvuII CAG'CTG
Cuts at: 1582 1744 2278 2638 1582
Size: 162 534 360 3797
Fragments arranged by size:
3797 534 360 162
RsrII CG'GwC_CG
Cuts at: 3048 3048
Size: 4853
SacI G_AGCT'C
Cuts at: 123 656 992 1867 123
Size: 533 336 875 3109
Fragments arranged by size:
3109 875 533 336
SalI G'TCGA_C
Cuts at: 1444 1890 1444
Size: 446 4407
SapI GCTCTTCn'nnn_
Cuts at: 2876 3086 2876
Size: 210 4643
SbfI CC_TGCA'GG
Cuts at: 1900 1900
Size: 4853
ScaI AGT'ACT
Cuts at: 943 4370 943
Size: 3427 1426
SexAI A'CCwGG_T
Cuts at: 1772 1817 1772
Size: 45 4808
SfcI C'TryA_G
Cuts at: 33 613 994 1896 2581 3574 3765 33
Size: 580 381 902 685 993 191 1121
Fragments arranged by size:
1121 993 902 685 580 381 191
SfoI GGC'GCC
Cuts at: 597 2285 2533 597
Size: 1688 248 2917
Fragments arranged by size:
2917 1688 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4853
SmaI CCC'GGG
Cuts at: 1875 1875
Size: 4853
SmlI C'TyrA_G
Cuts at: 655 1711 2008 2923 3415 3677 655
Size: 1056 297 915 492 262 1831
Fragments arranged by size:
1831 1056 915 492 297 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4853
SphI G_CATG'C
Cuts at: 376 1906 2406 376
Size: 1530 500 2823
Fragments arranged by size:
2823 1530 500
StuI AGG'CCT
Cuts at: 775 775
Size: 4853
StyI C'CwwG_G
Cuts at: 107 414 532 1544 1987 2341 107
Size: 307 118 1012 443 354 2619
Fragments arranged by size:
2619 1012 443 354 307 118
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 796 876 1155 2436 2569 3028 3237 796
Size: 80 279 1281 133 459 209 2412
Fragments arranged by size:
2412 1281 459 279 209 133 80
TatI w'GTAC_w
Cuts at: 269 860 941 4368 269
Size: 591 81 3427 754
Fragments arranged by size:
3427 754 591 81
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 385 417 643 675 4370 4402 385
Size: 32 226 32 3695 32 836
Fragments arranged by size:
3695 836 226 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2650 2650
Size: 4853
XbaI T'CTAG_A
Cuts at: 1884 1884
Size: 4853
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1359 1512 2348 1359
Size: 153 836 3864
Fragments arranged by size:
3864 836 153
XhoI C'TCGA_G
Cuts at: 655 655
Size: 4853
XmaI C'CCGG_G
Cuts at: 1873 1873
Size: 4853
XmnI GAAnn'nnTTC
Cuts at: 202 202
Size: 4853
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AleI
AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
AvrII BamHI BanI BanII BbeI BbsI BbvCI BciVI
BclI BglII BlpI Bme1580I BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI
BstEII BstYI Bsu36I BtgI BtgZI BtsI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NheI NspI PciI PflMI PfoI PmeI
PpiI PpuMI PsiI PspOMI PspXI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SexAI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AlfI AscI BaeI BcgI
BfrBI BglI BmgBI BsiWI BsmI BsmBI BsrGI BstXI
BstZ17I ClaI CspCI EcoRV FseI FspAI HindIII HpaI
MfeI NdeI NotI NruI NsiI PacI PasI PmlI
PshAI PsrI SacII SanDI SfiI SgrAI SnaBI SrfI
SspI SwaI ZraI