Restriction map for
pF1KB6101
(Circular) (Six-base) MAPSORT of: pF1KB6101.seq Check: 5953 from: 1 to: 4844
pF1KB6101 4844 bp
With 182 enzymes: SgfI *
October 7, 2009 14:19 ..
AatII G_ACGT'C
Cuts at: 962 980 962
Size: 18 4826
AccI GT'mk_AC
Cuts at: 939 1154 1882 939
Size: 215 728 3901
Fragments arranged by size:
3901 728 215
Acc65I G'GTAC_C
Cuts at: 1860 1860
Size: 4844
AclI AA'CG_TT
Cuts at: 195 4684 195
Size: 4489 355
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1441 2669 3101 3848 4116 1441
Size: 1228 432 747 268 2169
Fragments arranged by size:
2169 1228 747 432 268
AflIII A'CryG_T
Cuts at: 1280 2196 3300 4277 1280
Size: 916 1104 977 1847
Fragments arranged by size:
1847 1104 977 916
AgeI A'CCGG_T
Cuts at: 3232 3232
Size: 4844
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 602 602
Size: 4844
AlwNI CAG_nnn'CTG
Cuts at: 1225 2149 3716 1225
Size: 924 1567 2353
Fragments arranged by size:
2353 1567 924
ApaI G_GGCC'C
Cuts at: 1508 1508
Size: 4844
ApaLI G'TGCA_C
Cuts at: 3614 3614
Size: 4844
ApoI r'AATT_y
Cuts at: 1848 4197 4453 1848
Size: 2349 256 2239
Fragments arranged by size:
2349 2239 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4844
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4844
AvaI C'yCGr_G
Cuts at: 963 1775 1864 963
Size: 812 89 3943
Fragments arranged by size:
3943 812 89
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1269 1302 1269
Size: 33 4811
BamHI G'GATC_C
Cuts at: 1368 1675 1869 1368
Size: 307 194 4343
Fragments arranged by size:
4343 307 194
BanI G'GyrC_C
Cuts at: 124 1792 1860 2274 2522 2557 124
Size: 1668 68 414 248 35 2411
Fragments arranged by size:
2411 1668 414 248 68 35
BanII G_rGCy'C
Cuts at: 119 913 1498 1508 1858 2888 119
Size: 794 585 10 350 1030 2075
Fragments arranged by size:
2075 1030 794 585 350 10
BbeI G_GCGC'C
Cuts at: 128 2278 2526 128
Size: 2150 248 2446
Fragments arranged by size:
2446 2150 248
BbvCI CC'TCA_GC
Cuts at: 642 642
Size: 4844
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 459 493 1379 1413 1532 1566 1577 1611 459
Size: 34 886 34 119 34 11 34 3692
Fragments arranged by size:
3692 886 119 34 34 34 34 11
BciVI GTATCCnnnnn_n'
Cuts at: 1297 2742 3503 4844 1297
Size: 1445 761 1341 1297
Fragments arranged by size:
1445 1341 1297 761
BclI T'GATC_A
Cuts at: 2364 2364
Size: 4844
BglI GCCn_nnn'nGGC
Cuts at: 589 589
Size: 4844
BglII A'GATC_T
Cuts at: 1418 1475 2359 4396 1418
Size: 57 884 2037 1866
Fragments arranged by size:
2037 1866 884 57
BlpI GC'TnA_GC
Cuts at: 572 1956 572
Size: 1384 3460
Bme1580I G_kGCm'C
Cuts at: 1508 2469 2562 3618 1508
Size: 961 93 1056 2734
Fragments arranged by size:
2734 1056 961 93
BmgBI CAC'GTC
Cuts at: 531 531
Size: 4844
BmrI ACTGGGnnnn_n'
Cuts at: 1312 1511 2056 2137 2472 4616 1312
Size: 199 545 81 335 2144 1540
Fragments arranged by size:
2144 1540 545 335 199 81
BmtI G_CTAG'C
Cuts at: 850 850
Size: 4844
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 583 3211 583
Size: 2628 2216
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 292 403 881 1443 292
Size: 111 478 562 3693
Fragments arranged by size:
3693 562 478 111
Bpu10I CC'TnA_GC
Cuts at: 642 642
Size: 4844
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2020 2899 3391 3689 2020
Size: 879 492 298 3175
Fragments arranged by size:
3175 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4548 4548
Size: 4844
BsaAI yAC'GTr
Cuts at: 2197 2197
Size: 4844
BsaBI GATnn'nnATC
Cuts at: 6 454 2358 4395 6
Size: 448 1904 2037 455
Fragments arranged by size:
2037 1904 455 448
BsaHI Gr'CG_yC
Cuts at: 125 515 959 977 1592 2275 2523 3225 4721
Size: 390 444 18 615 683 248 702 1496
Cuts at: 4721 125
Size: 248
Fragments arranged by size:
1496 702 683 615 444 390 248 248
18
BsaWI w'CCGG_w
Cuts at: 853 2033 2554 3232 3506 3653 4064 853
Size: 1180 521 678 274 147 411 1633
Fragments arranged by size:
1633 1180 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 198 228 895 925 1510 1540 1772 1802 198
Size: 30 667 30 585 30 232 30 3240
Fragments arranged by size:
3240 667 585 232 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1793 1793
Size: 4844
BseYI C'CCAG_C
Cuts at: 917 2269 3027 3604 4100 917
Size: 1352 758 577 496 1661
Fragments arranged by size:
1661 1352 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 556 1317 1581 556
Size: 761 264 3819
Fragments arranged by size:
3819 761 264
BsiEI CG_ry'CG
Cuts at: 104 401 486 2386 2432 3640 104
Size: 297 85 1900 46 1208 1308
Fragments arranged by size:
1900 1308 1208 297 85 46
BsiHKAI G_wGCw'C
Cuts at: 119 714 1858 2636 2826 3618 119
Size: 595 1144 778 190 792 1345
Fragments arranged by size:
1345 1144 792 778 595 190
BsmI GAATG_Cn'
Cuts at: 553 553
Size: 4844
Bsp1286I G_dGCh'C
Cuts at: 119 714 913 1498 1508 1858 2469 2562 2636
Size: 595 199 585 10 350 611 93 74
Cuts at: 2636 2826 2888 3618 119
Size: 190 62 730 1345
Fragments arranged by size:
1345 730 611 595 585 350 199 190
93 74 62 10
BspEI T'CCGG_A
Cuts at: 853 4064 853
Size: 3211 1633
BspHI T'CATG_A
Cuts at: 4020 4020
Size: 4844
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1894 2108 2410 2791 1894
Size: 214 302 381 3947
Fragments arranged by size:
3947 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3136 3190 4674 4839 50
Size: 3086 54 1484 165 55
Fragments arranged by size:
3086 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2756 2756
Size: 4844
BsrFI r'CCGG_y
Cuts at: 396 646 2842 3023 3232 396
Size: 250 2196 181 209 2008
Fragments arranged by size:
2196 2008 250 209 181
BssSI C'ACGA_G
Cuts at: 922 3115 3473 922
Size: 2193 358 2293
Fragments arranged by size:
2293 2193 358
BstAPI GCAn_nnn'nTGC
Cuts at: 2221 2221
Size: 4844
BstBI TT'CG_AA
Cuts at: 3205 3205
Size: 4844
BstEII G'GTnAC_C
Cuts at: 1063 1063
Size: 4844
BstYI r'GATC_y
Cuts at: 1 856 1368 1418 1475 1675 1827 1869 2336
Size: 855 512 50 57 200 152 42 467
Cuts at: 2336 2359 2694 2940 3952 4038 4050 4396 1
Size: 23 335 246 1012 86 12 346 449
Fragments arranged by size:
1012 855 512 467 449 346 335 246
200 152 86 57 50 42 23 12
BstZ17I GTA'TAC
Cuts at: 940 940
Size: 4844
Bsu36I CC'TnA_GG
Cuts at: 1080 1080
Size: 4844
BtgI C'CryG_G
Cuts at: 611 686 611
Size: 75 4769
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 224 2794 2975 3138 224
Size: 2570 181 163 1930
Fragments arranged by size:
2570 1930 181 163
BtsI GCAGTG_nn'
Cuts at: 2087 2087
Size: 4844
DraI TTT'AAA
Cuts at: 1843 1843
Size: 4844
DrdI GACnn_nn'nnGTC
Cuts at: 1318 1690 2550 3408 1318
Size: 372 860 858 2754
Fragments arranged by size:
2754 860 858 372
EaeI y'GGCC_r
Cuts at: 398 483 608 1070 1667 2429 2603 2994 3021
Size: 85 125 462 597 762 174 391 27
Cuts at: 3021 4366 398
Size: 1345 876
Fragments arranged by size:
1345 876 762 597 462 391 174 125
85 27
EagI C'GGCC_G
Cuts at: 398 483 2429 398
Size: 85 1946 2813
Fragments arranged by size:
2813 1946 85
EarI CTCTTCn'nnn_
Cuts at: 135 153 695 2867 3077 135
Size: 18 542 2172 210 1902
Fragments arranged by size:
2172 1902 542 210 18
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3052 3362 3508 4654 3052
Size: 310 146 1146 3242
Fragments arranged by size:
3242 1146 310 146
EcoICRI GAG'CTC
Cuts at: 117 1856 117
Size: 1739 3105
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 292 403 881 1441 1443 2669 3101 3848 4116
Size: 111 478 560 2 1226 432 747 268
Cuts at: 4116 292
Size: 1020
Fragments arranged by size:
1226 1020 747 560 478 432 268 111
2
EcoO109I rG'GnC_Cy
Cuts at: 426 1096 1983 426
Size: 670 887 3287
Fragments arranged by size:
3287 887 670
EcoRI G'AATT_C
Cuts at: 1848 1848
Size: 4844
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2863 2863
Size: 4844
FspI TGC'GCA
Cuts at: 2625 2625
Size: 4844
HaeII r_GCGC'y
Cuts at: 128 2278 2526 3548 4527 128
Size: 2150 248 1022 979 445
Fragments arranged by size:
2150 1022 979 445 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 487 519 754 786 967 999 1704 1736 2686
Size: 32 235 32 181 32 705 32 950
Cuts at: 2686 2716 2718 2748 487
Size: 30 2 30 2583
Fragments arranged by size:
2583 950 705 235 181 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 1353 1883 1353
Size: 530 4314
KasI G'GCGC_C
Cuts at: 124 2274 2522 124
Size: 2150 248 2446
Fragments arranged by size:
2446 2150 248
KpnI G_GTAC'C
Cuts at: 1864 1864
Size: 4844
MluI A'CGCG_T
Cuts at: 4277 4277
Size: 4844
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 395 1553 1829 3515 3699 395
Size: 1158 276 1686 184 1540
Fragments arranged by size:
1686 1540 1158 276 184
MscI TGG'CCA
Cuts at: 1072 1669 2605 1072
Size: 597 936 3311
Fragments arranged by size:
3311 936 597
MslI CAynn'nnrTG
Cuts at: 1279 2833 2960 1279
Size: 1554 127 3163
Fragments arranged by size:
3163 1554 127
MspA1I CmG'CkG
Cuts at: 1955 2269 2629 3642 3887 1955
Size: 314 360 1013 245 2912
Fragments arranged by size:
2912 1013 360 314 245
NaeI GCC'GGC
Cuts at: 648 3025 648
Size: 2377 2467
NarI GG'CG_CC
Cuts at: 125 2275 2523 125
Size: 2150 248 2446
Fragments arranged by size:
2446 2150 248
NdeI CA'TA_TG
Cuts at: 1029 1029
Size: 4844
NgoMIV G'CCGG_C
Cuts at: 646 3023 646
Size: 2377 2467
NheI G'CTAG_C
Cuts at: 846 846
Size: 4844
NspI r_CATG'y
Cuts at: 1284 1897 2397 3304 1284
Size: 613 500 907 2824
Fragments arranged by size:
2824 907 613 500
PciI A'CATG_T
Cuts at: 1280 3300 1280
Size: 2020 2824
PflMI CCAn_nnn'nTGG
Cuts at: 1364 1490 1364
Size: 126 4718
PfoI T'CCnGG_A
Cuts at: 308 308
Size: 4844
PmeI GTTT'AAAC
Cuts at: 1843 1843
Size: 4844
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3983 4015 3983
Size: 32 4812
PspOMI G'GGCC_C
Cuts at: 1504 1504
Size: 4844
PstI C_TGCA'G
Cuts at: 156 434 1891 2576 156
Size: 278 1457 685 2424
Fragments arranged by size:
2424 1457 685 278
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4844
PvuII CAG'CTG
Cuts at: 2269 2629 2269
Size: 360 4484
RsrII CG'GwC_CG
Cuts at: 3039 3039
Size: 4844
SacI G_AGCT'C
Cuts at: 119 1858 119
Size: 1739 3105
SalI G'TCGA_C
Cuts at: 1881 1881
Size: 4844
SapI GCTCTTCn'nnn_
Cuts at: 2867 3077 2867
Size: 210 4634
SbfI CC_TGCA'GG
Cuts at: 156 1891 156
Size: 1735 3109
ScaI AGT'ACT
Cuts at: 1663 4361 1663
Size: 2698 2146
SfcI C'TryA_G
Cuts at: 33 152 430 1269 1632 1887 2572 3565 3756
Size: 119 278 839 363 255 685 993 191
Cuts at: 3756 33
Size: 1121
Fragments arranged by size:
1121 993 839 685 363 278 255 191
119
SfoI GGC'GCC
Cuts at: 126 2276 2524 126
Size: 2150 248 2446
Fragments arranged by size:
2446 2150 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4844
SmaI CCC'GGG
Cuts at: 1866 1866
Size: 4844
SmlI C'TyrA_G
Cuts at: 1999 2914 3406 3668 1999
Size: 915 492 262 3175
Fragments arranged by size:
3175 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4844
SphI G_CATG'C
Cuts at: 1897 2397 1897
Size: 500 4344
StyI C'CwwG_G
Cuts at: 1978 2332 1978
Size: 354 4490
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 127 931 1615 1780 2427 2560 3019 3228 127
Size: 804 684 165 647 133 459 209 1743
Fragments arranged by size:
1743 804 684 647 459 209 165 133
TatI w'GTAC_w
Cuts at: 1661 4359 1661
Size: 2698 2146
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 198 230 1772 1804 4361 4393 198
Size: 32 1542 32 2557 32 649
Fragments arranged by size:
2557 1542 649 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2641 2641
Size: 4844
XbaI T'CTAG_A
Cuts at: 1875 1875
Size: 4844
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1536 2339 1536
Size: 803 4041
XmaI C'CCGG_G
Cuts at: 1864 1864
Size: 4844
ZraI GAC'GTC
Cuts at: 960 978 960
Size: 18 4826
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AgeI AlfI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI
BamHI BanI BanII BbeI BbvCI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstEII BstYI BstZ17I Bsu36I BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NdeI NgoMIV NheI NspI PciI PflMI PfoI PmeI
PpiI PspOMI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI ZraI
Enzymes that do not cut:
AarI AfeI AflII AhdI AleI AloI AscI AvrII
BbsI BfrBI BsiWI BsmBI BsrGI BssHII BstXI ClaI
CspCI DraIII EcoNI EcoRV FseI FspAI HindIII HpaI
MfeI NcoI NotI NruI NsiI PacI PasI PmlI
PpuMI PshAI PsiI PspXI PsrI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI
XmnI