Restriction map for
pF1KB6013
(Circular) (Six-base) MAPSORT of: pF1KB6013.seq Check: 5786 from: 1 to: 4841
pF1KB6013 4841 bp
With 182 enzymes: SgfI *
April 24, 2009 13:05 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 141 298 973 141
Size: 157 675 4009
Fragments arranged by size:
4009 675 157
AatII G_ACGT'C
Cuts at: 1811 1811
Size: 4841
AccI GT'mk_AC
Cuts at: 1879 1879
Size: 4841
Acc65I G'GTAC_C
Cuts at: 1857 1857
Size: 4841
AclI AA'CG_TT
Cuts at: 4681 4681
Size: 4841
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1114 2666 3098 3451 4113 1114
Size: 1552 432 353 662 1842
Fragments arranged by size:
1842 1552 662 432 353
AfeI AGC'GCT
Cuts at: 161 1438 161
Size: 1277 3564
AflIII A'CryG_T
Cuts at: 436 2193 3993 4274 436
Size: 1757 1800 281 1003
Fragments arranged by size:
1800 1757 1003 281
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1163 1671 1738 1163
Size: 508 67 4266
Fragments arranged by size:
4266 508 67
AlwNI CAG_nnn'CTG
Cuts at: 1481 2146 3584 1481
Size: 665 1438 2738
Fragments arranged by size:
2738 1438 665
ApaI G_GGCC'C
Cuts at: 117 412 1289 117
Size: 295 877 3669
Fragments arranged by size:
3669 877 295
ApaLI G'TGCA_C
Cuts at: 757 3679 757
Size: 2922 1919
ApoI r'AATT_y
Cuts at: 1845 4194 4450 1845
Size: 2349 256 2236
Fragments arranged by size:
2349 2236 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4841
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4841
AvaI C'yCGr_G
Cuts at: 184 1539 1861 184
Size: 1355 322 3164
Fragments arranged by size:
3164 1355 322
BamHI G'GATC_C
Cuts at: 1866 1866
Size: 4841
BanI G'GyrC_C
Cuts at: 189 1857 2271 2519 2554 189
Size: 1668 414 248 35 2476
Fragments arranged by size:
2476 1668 414 248 35
BanII G_rGCy'C
Cuts at: 117 412 469 533 1289 1855 2885 117
Size: 295 57 64 756 566 1030 2073
Fragments arranged by size:
2073 1030 756 566 295 64 57
BbeI G_GCGC'C
Cuts at: 2275 2523 2275
Size: 248 4593
BbsI GAAGACnn'nnnn_
Cuts at: 1804 1804
Size: 4841
BciVI GTATCCnnnnn_n'
Cuts at: 2739 3795 4841 2739
Size: 1056 1046 2739
Fragments arranged by size:
2739 1056 1046
BclI T'GATC_A
Cuts at: 2361 2361
Size: 4841
BglI GCCn_nnn'nGGC
Cuts at: 112 416 1064 1331 112
Size: 304 648 267 3622
Fragments arranged by size:
3622 648 304 267
BglII A'GATC_T
Cuts at: 2356 4393 2356
Size: 2037 2804
BlpI GC'TnA_GC
Cuts at: 1953 1953
Size: 4841
Bme1580I G_kGCm'C
Cuts at: 117 194 412 761 1289 1417 1761 2466 2559
Size: 77 218 349 528 128 344 705 93
Cuts at: 2559 3683 117
Size: 1124 1275
Fragments arranged by size:
1275 1124 705 528 349 344 218 128
93 77
BmgBI CAC'GTC
Cuts at: 393 393
Size: 4841
BmrI ACTGGGnnnn_n'
Cuts at: 406 1653 2053 2134 2469 4613 406
Size: 1247 400 81 335 2144 634
Fragments arranged by size:
2144 1247 634 400 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 615 1554 3208 615
Size: 939 1654 2248
Fragments arranged by size:
2248 1654 939
Bpu10I CC'TnA_GC
Cuts at: 716 716
Size: 4841
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1579 1798 2017 2896 3610 3908 1579
Size: 219 219 879 714 298 2512
Fragments arranged by size:
2512 879 714 298 219 219
BsaI GGTCTCn'nnnn_
Cuts at: 143 319 670 703 1207 4545 143
Size: 176 351 33 504 3338 439
Fragments arranged by size:
3338 504 439 351 176 33
BsaAI yAC'GTr
Cuts at: 1638 2194 1638
Size: 556 4285
BsaBI GATnn'nnATC
Cuts at: 6 2355 4392 6
Size: 2349 2037 455
Fragments arranged by size:
2349 2037 455
BsaHI Gr'CG_yC
Cuts at: 1808 2272 2520 3222 4718 1808
Size: 464 248 702 1496 1931
Fragments arranged by size:
1931 1496 702 464 248
BsaWI w'CCGG_w
Cuts at: 2030 2551 3229 3640 3787 4061 2030
Size: 521 678 411 147 274 2810
Fragments arranged by size:
2810 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 829 859 829
Size: 30 4811
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1420 1543 1420
Size: 123 4718
BseYI C'CCAG_C
Cuts at: 143 532 917 938 952 985 1310 2266 3024
Size: 389 385 21 14 33 325 956 758
Cuts at: 3024 3689 4097 143
Size: 665 408 887
Fragments arranged by size:
956 887 758 665 408 389 385 325
33 21 14
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 951 1284 951
Size: 333 4508
BsiEI CG_ry'CG
Cuts at: 104 1085 2383 2429 3659 104
Size: 981 1298 46 1230 1286
Fragments arranged by size:
1298 1286 1230 981 46
BsiHKAI G_wGCw'C
Cuts at: 289 761 1855 2633 2823 3683 289
Size: 472 1094 778 190 860 1447
Fragments arranged by size:
1447 1094 860 778 472 190
BsmBI CGTCTCn'nnnn_
Cuts at: 301 301
Size: 4841
Bsp1286I G_dGCh'C
Cuts at: 117 194 289 412 469 533 761 1289 1417
Size: 77 95 123 57 64 228 528 128
Cuts at: 1417 1761 1855 2466 2559 2633 2823 2885 3683
Size: 344 94 611 93 74 190 62 798
Cuts at: 3683 117
Size: 1275
Fragments arranged by size:
1275 798 611 528 344 228 190 128
123 95 94 93 77 74 64 62
57
BspHI T'CATG_A
Cuts at: 3273 3273
Size: 4841
BspMI ACCTGCnnnn'nnnn_
Cuts at: 141 298 550 637 973 1891 2105 2407 2788
Size: 157 252 87 336 918 214 302 381
Cuts at: 2788 141
Size: 2194
Fragments arranged by size:
2194 918 381 336 302 252 214 157
87
BsrBI CCG'CTC
Cuts at: 50 1247 1686 3133 3187 4671 4836 50
Size: 1197 439 1447 54 1484 165 55
Fragments arranged by size:
1484 1447 1197 439 165 55 54
BsrDI GCAATG_nn'
Cuts at: 911 1377 2753 911
Size: 466 1376 2999
Fragments arranged by size:
2999 1376 466
BsrFI r'CCGG_y
Cuts at: 128 235 812 2839 3020 128
Size: 107 577 2027 181 1949
Fragments arranged by size:
2027 1949 577 181 107
BssSI C'ACGA_G
Cuts at: 3112 3820 3112
Size: 708 4133
BstAPI GCAn_nnn'nTGC
Cuts at: 1756 2218 1756
Size: 462 4379
BstBI TT'CG_AA
Cuts at: 3202 3202
Size: 4841
BstEII G'GTnAC_C
Cuts at: 768 903 768
Size: 135 4706
BstXI CCAn_nnnn'nTGG
Cuts at: 889 889
Size: 4841
BstYI r'GATC_y
Cuts at: 1 956 1866 2333 2356 2691 2937 3243 3255
Size: 955 910 467 23 335 246 306 12
Cuts at: 3255 3341 4393 1
Size: 86 1052 449
Fragments arranged by size:
1052 955 910 467 449 335 306 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 994 994
Size: 4841
BtgI C'CryG_G
Cuts at: 773 773
Size: 4841
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2791 2972 3135 2791
Size: 181 163 4497
Fragments arranged by size:
4497 181 163
BtsI GCAGTG_nn'
Cuts at: 1185 2084 1185
Size: 899 3942
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 336 371 336
Size: 35 4806
DraI TTT'AAA
Cuts at: 1840 1840
Size: 4841
DrdI GACnn_nn'nnGTC
Cuts at: 2547 3891 2547
Size: 1344 3497
EaeI y'GGCC_r
Cuts at: 489 617 1082 1182 1256 1718 2426 2600 2991
Size: 128 465 100 74 462 708 174 391
Cuts at: 2991 3018 4363 489
Size: 27 1345 967
Fragments arranged by size:
1345 967 708 465 462 391 174 128
100 74 27
EagI C'GGCC_G
Cuts at: 1082 2426 1082
Size: 1344 3497
EarI CTCTTCn'nnn_
Cuts at: 1118 2864 3074 1118
Size: 1746 210 2885
Fragments arranged by size:
2885 1746 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3049 3791 3937 4651 3049
Size: 742 146 714 3239
Fragments arranged by size:
3239 742 714 146
EcoICRI GAG'CTC
Cuts at: 1853 1853
Size: 4841
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1114 2666 3098 3451 4113 1114
Size: 1552 432 353 662 1842
Fragments arranged by size:
1842 1552 662 432 353
EcoNI CCTnn'n_nnAGG
Cuts at: 1422 1606 1422
Size: 184 4657
EcoO109I rG'GnC_Cy
Cuts at: 114 408 990 1285 1286 1569 1980 114
Size: 294 582 295 1 283 411 2975
Fragments arranged by size:
2975 582 411 295 294 283 1
EcoRI G'AATT_C
Cuts at: 1845 1845
Size: 4841
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2860 2860
Size: 4841
FseI GG_CCGG'CC
Cuts at: 816 816
Size: 4841
FspI TGC'GCA
Cuts at: 2622 2622
Size: 4841
HaeII r_GCGC'y
Cuts at: 163 211 1440 2275 2523 3753 4524 163
Size: 48 1229 835 248 1230 771 480
Fragments arranged by size:
1230 1229 835 771 480 248 48
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2683 2713 2715 2745 2683
Size: 30 2 30 4779
Fragments arranged by size:
4779 30 30 2
HincII GTy'rAC
Cuts at: 1880 1880
Size: 4841
HindIII A'AGCT_T
Cuts at: 1774 1774
Size: 4841
KasI G'GCGC_C
Cuts at: 2271 2519 2271
Size: 248 4593
KpnI G_GTAC'C
Cuts at: 1861 1861
Size: 4841
MluI A'CGCG_T
Cuts at: 4274 4274
Size: 4841
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 827 3600 3784 827
Size: 2773 184 1884
Fragments arranged by size:
2773 1884 184
MscI TGG'CCA
Cuts at: 491 1720 2602 491
Size: 1229 882 2730
Fragments arranged by size:
2730 1229 882
MslI CAynn'nnrTG
Cuts at: 391 887 1145 1693 1720 2830 2957 391
Size: 496 258 548 27 1110 127 2275
Fragments arranged by size:
2275 1110 548 496 258 127 27
MspA1I CmG'CkG
Cuts at: 518 575 921 942 1011 1136 1254 1952 2266
Size: 57 346 21 69 125 118 698 314
Cuts at: 2266 2626 3410 3655 518
Size: 360 784 245 1704
Fragments arranged by size:
1704 784 698 360 346 314 245 125
118 69 57 21
NaeI GCC'GGC
Cuts at: 237 814 3022 237
Size: 577 2208 2056
Fragments arranged by size:
2208 2056 577
NarI GG'CG_CC
Cuts at: 2272 2520 2272
Size: 248 4593
NcoI C'CATG_G
Cuts at: 773 773
Size: 4841
NgoMIV G'CCGG_C
Cuts at: 235 812 3020 235
Size: 577 2208 2056
Fragments arranged by size:
2208 2056 577
NspI r_CATG'y
Cuts at: 423 440 1233 1652 1894 2394 3997 423
Size: 17 793 419 242 500 1603 1267
Fragments arranged by size:
1603 1267 793 500 419 242 17
PasI CC'CwG_GG
Cuts at: 1424 1424
Size: 4841
PciI A'CATG_T
Cuts at: 436 3993 436
Size: 3557 1284
PflMI CCAn_nnn'nTGG
Cuts at: 269 269
Size: 4841
PmeI GTTT'AAAC
Cuts at: 1840 1840
Size: 4841
PmlI CAC'GTG
Cuts at: 1638 1638
Size: 4841
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3287 3319 3287
Size: 32 4809
PpuMI rG'GwC_Cy
Cuts at: 990 1569 990
Size: 579 4262
PspOMI G'GGCC_C
Cuts at: 113 408 1285 113
Size: 295 877 3669
Fragments arranged by size:
3669 877 295
PstI C_TGCA'G
Cuts at: 295 461 947 1041 1283 1888 2573 295
Size: 166 486 94 242 605 685 2563
Fragments arranged by size:
2563 685 605 486 242 166 94
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4841
PvuII CAG'CTG
Cuts at: 921 942 2266 2626 921
Size: 21 1324 360 3136
Fragments arranged by size:
3136 1324 360 21
RsrII CG'GwC_CG
Cuts at: 3036 3036
Size: 4841
SacI G_AGCT'C
Cuts at: 1855 1855
Size: 4841
SalI G'TCGA_C
Cuts at: 1878 1878
Size: 4841
SapI GCTCTTCn'nnn_
Cuts at: 1118 2864 3074 1118
Size: 1746 210 2885
Fragments arranged by size:
2885 1746 210
SbfI CC_TGCA'GG
Cuts at: 295 1283 1888 295
Size: 988 605 3248
Fragments arranged by size:
3248 988 605
ScaI AGT'ACT
Cuts at: 4358 4358
Size: 4841
SfcI C'TryA_G
Cuts at: 33 291 457 943 1037 1279 1884 2569 3537
Size: 258 166 486 94 242 605 685 968
Cuts at: 3537 3728 33
Size: 191 1146
Fragments arranged by size:
1146 968 685 605 486 258 242 191
166 94
SfoI GGC'GCC
Cuts at: 2273 2521 2273
Size: 248 4593
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4841
SmaI CCC'GGG
Cuts at: 1863 1863
Size: 4841
SmlI C'TyrA_G
Cuts at: 1594 1813 1996 2911 3625 3887 1594
Size: 219 183 915 714 262 2548
Fragments arranged by size:
2548 915 714 262 219 183
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4841
SphI G_CATG'C
Cuts at: 423 1233 1652 1894 2394 423
Size: 810 419 242 500 2870
Fragments arranged by size:
2870 810 500 419 242
StuI AGG'CCT
Cuts at: 595 1225 1399 595
Size: 630 174 4037
Fragments arranged by size:
4037 630 174
StyI C'CwwG_G
Cuts at: 118 425 773 1975 2329 118
Size: 307 348 1202 354 2630
Fragments arranged by size:
2630 1202 354 348 307
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 261 756 766 1540 2424 2557 3016 3225 261
Size: 495 10 774 884 133 459 209 1877
Fragments arranged by size:
1877 884 774 495 459 209 133 10
TatI w'GTAC_w
Cuts at: 4356 4356
Size: 4841
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 304 336 1764 1796 4358 4390 304
Size: 32 1428 32 2562 32 755
Fragments arranged by size:
2562 1428 755 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1495 2638 1495
Size: 1143 3698
XbaI T'CTAG_A
Cuts at: 1872 1872
Size: 4841
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 270 369 2336 270
Size: 99 1967 2775
Fragments arranged by size:
2775 1967 99
XmaI C'CCGG_G
Cuts at: 1861 1861
Size: 4841
ZraI GAC'GTC
Cuts at: 1809 1809
Size: 4841
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BplI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FseI FspI HaeII Hin4I HincII HindIII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PasI PciI PflMI PmeI PmlI
PpiI PpuMI PspOMI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI ZraI
Enzymes that do not cut:
AflII AgeI AhdI AleI AloI AscI AvrII BaeI
BbvCI BcgI BfrBI BmtI BpmI BsiWI BsmI BspEI
BsrGI BssHII BstZ17I ClaI DraIII EcoRV FspAI HpaI
MfeI NdeI NheI NotI NruI NsiI PacI PfoI
PshAI PsiI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI SspI SwaI XhoI XmnI