Restriction map for
pF1KB6010
(Circular) (Six-base) MAPSORT of: pF1KB6010.seq Check: 2404 from: 1 to: 6806
pF1KB6010 6806 bp
With 182 enzymes: SgfI *
March 2, 2010 14:26 ..
AccI GT'mk_AC
Cuts at: 2551 3844 2551
Size: 1293 5513
Acc65I G'GTAC_C
Cuts at: 3822 3822
Size: 6806
AclI AA'CG_TT
Cuts at: 6646 6646
Size: 6806
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 575 913 1401 2484 3407 3757 4631 5063 5416
Size: 338 488 1083 923 350 874 432 353
Cuts at: 5416 6078 575
Size: 662 1303
Fragments arranged by size:
1303 1083 923 874 662 488 432 353
350 338
AflIII A'CryG_T
Cuts at: 2531 4158 5958 6239 2531
Size: 1627 1800 281 3098
Fragments arranged by size:
3098 1800 1627 281
AhdI GACnn_n'nnGTC
Cuts at: 3276 3276
Size: 6806
AleI CACnn'nnGTG
Cuts at: 2310 2310
Size: 6806
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1679 1952 1679
Size: 273 6533
AlwNI CAG_nnn'CTG
Cuts at: 4111 5549 4111
Size: 1438 5368
ApaI G_GGCC'C
Cuts at: 256 256
Size: 6806
ApaLI G'TGCA_C
Cuts at: 5644 5644
Size: 6806
ApoI r'AATT_y
Cuts at: 559 637 1016 1396 2098 2694 2843 3043 3093
Size: 78 379 380 702 596 149 200 50
Cuts at: 3093 3121 3243 3810 6159 6415 559
Size: 28 122 567 2349 256 950
Fragments arranged by size:
2349 950 702 596 567 380 379 256
200 149 122 78 50 28
AseI AT'TA_AT
Cuts at: 20 824 20
Size: 804 6002
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6806
AvaI C'yCGr_G
Cuts at: 3826 3826
Size: 6806
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1973 2006 1973
Size: 33 6773
BamHI G'GATC_C
Cuts at: 3831 3831
Size: 6806
BanI G'GyrC_C
Cuts at: 3822 4236 4484 4519 3822
Size: 414 248 35 6109
Fragments arranged by size:
6109 414 248 35
BanII G_rGCy'C
Cuts at: 256 3494 3820 4850 256
Size: 3238 326 1030 2212
Fragments arranged by size:
3238 2212 1030 326
BbeI G_GCGC'C
Cuts at: 4240 4488 4240
Size: 248 6558
BbsI GAAGACnn'nnnn_
Cuts at: 1470 1774 2598 1470
Size: 304 824 5678
Fragments arranged by size:
5678 824 304
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1010 1044 1010
Size: 34 6772
BciVI GTATCCnnnnn_n'
Cuts at: 693 4704 5760 6806 693
Size: 4011 1056 1046 693
Fragments arranged by size:
4011 1056 1046 693
BclI T'GATC_A
Cuts at: 2991 4326 2991
Size: 1335 5471
BfrBI ATG'CAT
Cuts at: 1116 2158 1116
Size: 1042 5764
BglII A'GATC_T
Cuts at: 4321 6358 4321
Size: 2037 4769
BlpI GC'TnA_GC
Cuts at: 2584 3918 2584
Size: 1334 5472
Bme1580I G_kGCm'C
Cuts at: 256 4431 4524 5648 256
Size: 4175 93 1124 1414
Fragments arranged by size:
4175 1414 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 1599 4018 4099 4434 6578 1599
Size: 2419 81 335 2144 1827
Fragments arranged by size:
2419 2144 1827 335 81
BmtI G_CTAG'C
Cuts at: 3256 3256
Size: 6806
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1417 5173 1417
Size: 3756 3050
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 104 1412 104
Size: 1308 5498
Bpu10I CC'TnA_GC
Cuts at: 2285 2285
Size: 6806
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3982 4861 5575 5873 3982
Size: 879 714 298 4915
Fragments arranged by size:
4915 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 6510 6510
Size: 6806
BsaAI yAC'GTr
Cuts at: 4159 4159
Size: 6806
BsaBI GATnn'nnATC
Cuts at: 6 140 2159 3572 4320 6357 6
Size: 134 2019 1413 748 2037 455
Fragments arranged by size:
2037 2019 1413 748 455 134
BsaHI Gr'CG_yC
Cuts at: 4237 4485 5187 6683 4237
Size: 248 702 1496 4360
Fragments arranged by size:
4360 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1955 3995 4516 5194 5605 5752 6026 1955
Size: 2040 521 678 411 147 274 2735
Fragments arranged by size:
2735 2040 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 971 1001 971
Size: 30 6776
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 299 1450 299
Size: 1151 5655
BseYI C'CCAG_C
Cuts at: 255 4231 4989 5654 6062 255
Size: 3976 758 665 408 999
Fragments arranged by size:
3976 999 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 2657 2665 3421 2657
Size: 8 756 6042
Fragments arranged by size:
6042 756 8
BsiEI CG_ry'CG
Cuts at: 104 4348 4394 5624 104
Size: 4244 46 1230 1286
Fragments arranged by size:
4244 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 1668 3820 4598 4788 5648 1668
Size: 2152 778 190 860 2826
Fragments arranged by size:
2826 2152 860 778 190
BsmI GAATG_Cn'
Cuts at: 969 969
Size: 6806
Bsp1286I G_dGCh'C
Cuts at: 256 1668 3494 3820 4431 4524 4598 4788 4850
Size: 1412 1826 326 611 93 74 190 62
Cuts at: 4850 5648 256
Size: 798 1414
Fragments arranged by size:
1826 1414 1412 798 611 326 190 93
74 62
BspEI T'CCGG_A
Cuts at: 1955 1955
Size: 6806
BspHI T'CATG_A
Cuts at: 3182 3521 5238 3182
Size: 339 1717 4750
Fragments arranged by size:
4750 1717 339
BspMI ACCTGCnnnn'nnnn_
Cuts at: 3856 4070 4372 4753 3856
Size: 214 302 381 5909
Fragments arranged by size:
5909 381 302 214
BsrBI CCG'CTC
Cuts at: 50 5098 5152 6636 6801 50
Size: 5048 54 1484 165 55
Fragments arranged by size:
5048 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 667 2566 3014 4718 667
Size: 1899 448 1704 2755
Fragments arranged by size:
2755 1899 1704 448
BsrFI r'CCGG_y
Cuts at: 1370 2105 4804 4985 1370
Size: 735 2699 181 3191
Fragments arranged by size:
3191 2699 735 181
BsrGI T'GTAC_A
Cuts at: 987 987
Size: 6806
BssSI C'ACGA_G
Cuts at: 5077 5785 5077
Size: 708 6098
BstAPI GCAn_nnn'nTGC
Cuts at: 4183 4183
Size: 6806
BstBI TT'CG_AA
Cuts at: 543 5167 543
Size: 4624 2182
BstXI CCAn_nnnn'nTGG
Cuts at: 337 337
Size: 6806
BstYI r'GATC_y
Cuts at: 1 1428 1952 3657 3831 4298 4321 4656 4902
Size: 1427 524 1705 174 467 23 335 246
Cuts at: 4902 5208 5220 5306 6358 1
Size: 306 12 86 1052 449
Fragments arranged by size:
1705 1427 1052 524 467 449 335 306
246 174 86 23 12
BstZ17I GTA'TAC
Cuts at: 2552 2552
Size: 6806
Bsu36I CC'TnA_GG
Cuts at: 3509 3509
Size: 6806
BtgI C'CryG_G
Cuts at: 593 1721 1811 3686 593
Size: 1128 90 1875 3713
Fragments arranged by size:
3713 1875 1128 90
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4756 4937 5100 4756
Size: 181 163 6462
Fragments arranged by size:
6462 181 163
BtsI GCAGTG_nn'
Cuts at: 1322 2189 2305 2317 3433 4049 1322
Size: 867 116 12 1116 616 4079
Fragments arranged by size:
4079 1116 867 616 116 12
DraI TTT'AAA
Cuts at: 975 1094 2390 3054 3092 3805 975
Size: 119 1296 664 38 713 3976
Fragments arranged by size:
3976 1296 713 664 119 38
DraIII CAC_nnn'GTG
Cuts at: 1630 1630
Size: 6806
DrdI GACnn_nn'nnGTC
Cuts at: 739 1145 4512 5856 739
Size: 406 3367 1344 1689
Fragments arranged by size:
3367 1689 1344 406
EaeI y'GGCC_r
Cuts at: 1372 4391 4565 4956 4983 6328 1372
Size: 3019 174 391 27 1345 1850
Fragments arranged by size:
3019 1850 1345 391 174 27
EagI C'GGCC_G
Cuts at: 4391 4391
Size: 6806
EarI CTCTTCn'nnn_
Cuts at: 1031 1420 1452 1613 1722 2027 2176 3201 3602
Size: 389 32 161 109 305 149 1025 401
Cuts at: 3602 4829 5039 1031
Size: 1227 210 2798
Fragments arranged by size:
2798 1227 1025 401 389 305 210 161
149 109 32
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 5014 5756 5902 6616 5014
Size: 742 146 714 5204
Fragments arranged by size:
5204 742 714 146
EcoICRI GAG'CTC
Cuts at: 3818 3818
Size: 6806
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 104 575 913 1401 1412 2484 3407 3757 4631
Size: 471 338 488 11 1072 923 350 874
Cuts at: 4631 5063 5416 6078 104
Size: 432 353 662 832
Fragments arranged by size:
1072 923 874 832 662 488 471 432
353 350 338 11
EcoO109I rG'GnC_Cy
Cuts at: 252 3945 252
Size: 3693 3113
EcoRI G'AATT_C
Cuts at: 559 637 3810 559
Size: 78 3173 3555
Fragments arranged by size:
3555 3173 78
EcoRV GAT'ATC
Cuts at: 195 2760 2802 195
Size: 2565 42 4199
Fragments arranged by size:
4199 2565 42
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1642 2394 4825 1642
Size: 752 2431 3623
Fragments arranged by size:
3623 2431 752
FspI TGC'GCA
Cuts at: 4587 4587
Size: 6806
HaeII r_GCGC'y
Cuts at: 4240 4488 5718 6489 4240
Size: 248 1230 771 4557
Fragments arranged by size:
4557 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 102 134 2789 2821 2914 2946 3262 3294 3516
Size: 32 2655 32 93 32 316 32 222
Cuts at: 3516 3534 3548 3566 3642 3674 4648 4678 4680
Size: 18 14 18 76 32 974 30 2
Cuts at: 4680 4710 102
Size: 30 2198
Fragments arranged by size:
2655 2198 974 316 222 93 76 32
32 32 32 32 30 30 18 18
14 2
HincII GTy'rAC
Cuts at: 2074 2174 2425 3311 3845 2074
Size: 100 251 886 534 5035
Fragments arranged by size:
5035 886 534 251 100
KasI G'GCGC_C
Cuts at: 4236 4484 4236
Size: 248 6558
KpnI G_GTAC'C
Cuts at: 3826 3826
Size: 6806
MfeI C'AATT_G
Cuts at: 3614 3614
Size: 6806
MluI A'CGCG_T
Cuts at: 6239 6239
Size: 6806
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1087 1757 5565 5749 1087
Size: 670 3808 184 2144
Fragments arranged by size:
3808 2144 670 184
MscI TGG'CCA
Cuts at: 4567 4567
Size: 6806
MslI CAynn'nnrTG
Cuts at: 646 826 1051 1532 1934 2310 3019 3032 3481
Size: 180 225 481 402 376 709 13 449
Cuts at: 3481 4795 4922 646
Size: 1314 127 2530
Fragments arranged by size:
2530 1314 709 481 449 402 376 225
180 127 13
MspA1I CmG'CkG
Cuts at: 1390 2752 3917 4231 4591 5375 5620 1390
Size: 1362 1165 314 360 784 245 2576
Fragments arranged by size:
2576 1362 1165 784 360 314 245
NaeI GCC'GGC
Cuts at: 4987 4987
Size: 6806
NarI GG'CG_CC
Cuts at: 4237 4485 4237
Size: 248 6558
NcoI C'CATG_G
Cuts at: 593 3686 593
Size: 3093 3713
NdeI CA'TA_TG
Cuts at: 521 521
Size: 6806
NgoMIV G'CCGG_C
Cuts at: 4985 4985
Size: 6806
NheI G'CTAG_C
Cuts at: 3252 3252
Size: 6806
NsiI A_TGCA'T
Cuts at: 1118 2160 1118
Size: 1042 5764
NspI r_CATG'y
Cuts at: 1537 1560 2003 2283 2535 3372 3859 4359 5962
Size: 23 443 280 252 837 487 500 1603
Cuts at: 5962 1537
Size: 2381
Fragments arranged by size:
2381 1603 837 500 487 443 280 252
23
PciI A'CATG_T
Cuts at: 2531 5958 2531
Size: 3427 3379
PflMI CCAn_nnn'nTGG
Cuts at: 1630 1630
Size: 6806
PmeI GTTT'AAAC
Cuts at: 3805 3805
Size: 6806
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5252 5284 5252
Size: 32 6774
PsiI TTA'TAA
Cuts at: 1175 2786 3572 1175
Size: 1611 786 4409
Fragments arranged by size:
4409 1611 786
PspOMI G'GGCC_C
Cuts at: 252 252
Size: 6806
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 893 925 893
Size: 32 6774
PstI C_TGCA'G
Cuts at: 1329 2312 2757 3853 4538 1329
Size: 983 445 1096 685 3597
Fragments arranged by size:
3597 1096 983 685 445
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6806
PvuII CAG'CTG
Cuts at: 1390 2752 4231 4591 1390
Size: 1362 1479 360 3605
Fragments arranged by size:
3605 1479 1362 360
RsrII CG'GwC_CG
Cuts at: 5001 5001
Size: 6806
SacI G_AGCT'C
Cuts at: 3820 3820
Size: 6806
SalI G'TCGA_C
Cuts at: 3843 3843
Size: 6806
SapI GCTCTTCn'nnn_
Cuts at: 1420 1613 3602 4829 5039 1420
Size: 193 1989 1227 210 3187
Fragments arranged by size:
3187 1989 1227 210 193
SbfI CC_TGCA'GG
Cuts at: 3853 3853
Size: 6806
ScaI AGT'ACT
Cuts at: 1888 6323 1888
Size: 4435 2371
SfcI C'TryA_G
Cuts at: 33 148 1325 1844 2308 2753 3341 3849 4534
Size: 115 1177 519 464 445 588 508 685
Cuts at: 4534 5502 5693 33
Size: 968 191 1146
Fragments arranged by size:
1177 1146 968 685 588 519 508 464
445 191 115
SfoI GGC'GCC
Cuts at: 4238 4486 4238
Size: 248 6558
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6806
SmaI CCC'GGG
Cuts at: 3828 3828
Size: 6806
SmlI C'TyrA_G
Cuts at: 3961 4876 5590 5852 3961
Size: 915 714 262 4915
Fragments arranged by size:
4915 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6806
SphI G_CATG'C
Cuts at: 1537 3372 3859 4359 1537
Size: 1835 487 500 3984
Fragments arranged by size:
3984 1835 500 487
SspI AAT'ATT
Cuts at: 971 1962 971
Size: 991 5815
StuI AGG'CCT
Cuts at: 2467 2467
Size: 6806
StyI C'CwwG_G
Cuts at: 593 3686 3940 4294 593
Size: 3093 254 354 3105
Fragments arranged by size:
3105 3093 354 254
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 449 4389 4522 4981 5190 449
Size: 3940 133 459 209 2065
Fragments arranged by size:
3940 2065 459 209 133
TatI w'GTAC_w
Cuts at: 987 1269 1886 2314 6321 987
Size: 282 617 428 4007 1472
Fragments arranged by size:
4007 1472 617 428 282
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1543 1575 1730 1762 6323 6355 1543
Size: 32 155 32 4561 32 1994
Fragments arranged by size:
4561 1994 155 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1469 4603 1469
Size: 3134 3672
XbaI T'CTAG_A
Cuts at: 1512 3837 1512
Size: 2325 4481
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4301 4301
Size: 6806
XmaI C'CCGG_G
Cuts at: 3826 3826
Size: 6806
XmnI GAAnn'nnTTC
Cuts at: 616 1316 2120 3779 616
Size: 700 804 1659 3643
Fragments arranged by size:
3643 1659 804 700
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AhdI AleI AlfI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI
BamHI BanI BanII BbeI BbsI BcgI BciVI BclI
BfrBI BglII BlpI Bme1580I BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstBI BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I
HincII KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NsiI
NspI PciI PflMI PmeI PpiI PsiI PspOMI PsrI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AloI AscI AvrII
BbvCI BglI BmgBI BsiWI BsmBI BssHII BstEII ClaI
CspCI EcoNI FseI FspAI HindIII HpaI NotI NruI
PacI PasI PfoI PmlI PpuMI PshAI PspXI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI SwaI XhoI
ZraI