Restriction map for
pF1KB6005
(Circular) (Six-base) MAPSORT of: pF1KB6005.seq Check: 7438 from: 1 to: 6362
pF1KB6005 6362 bp
With 182 enzymes: SgfI *
June 20, 2008 10:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2236 2236
Size: 6362
AatII G_ACGT'C
Cuts at: 2972 2972
Size: 6362
AccI GT'mk_AC
Cuts at: 430 1720 3400 430
Size: 1290 1680 3392
Fragments arranged by size:
3392 1680 1290
Acc65I G'GTAC_C
Cuts at: 3378 3378
Size: 6362
AclI AA'CG_TT
Cuts at: 6202 6202
Size: 6362
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 344 641 1197 1262 1897 1946 1970 3313 4187
Size: 297 556 65 635 49 24 1343 874
Cuts at: 4187 4619 5366 5634 344
Size: 432 747 268 1072
Fragments arranged by size:
1343 1072 874 747 635 556 432 297
268 65 49 24
AflII C'TTAA_G
Cuts at: 3262 3262
Size: 6362
AflIII A'CryG_T
Cuts at: 2273 3714 4818 5795 2273
Size: 1441 1104 977 2840
Fragments arranged by size:
2840 1441 1104 977
AgeI A'CCGG_T
Cuts at: 4750 4750
Size: 6362
AleI CACnn'nnGTG
Cuts at: 1228 1993 3062 1228
Size: 765 1069 4528
Fragments arranged by size:
4528 1069 765
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2135 2167 2135
Size: 32 6330
AlwNI CAG_nnn'CTG
Cuts at: 814 1234 2509 2605 3667 5234 814
Size: 420 1275 96 1062 1567 1942
Fragments arranged by size:
1942 1567 1275 1062 420 96
ApaI G_GGCC'C
Cuts at: 115 770 2542 115
Size: 655 1772 3935
Fragments arranged by size:
3935 1772 655
ApaLI G'TGCA_C
Cuts at: 5132 5132
Size: 6362
ApoI r'AATT_y
Cuts at: 1877 3366 5715 5971 1877
Size: 1489 2349 256 2268
Fragments arranged by size:
2349 2268 1489 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 6362
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6362
AvaI C'yCGr_G
Cuts at: 371 800 2198 3382 371
Size: 429 1398 1184 3351
Fragments arranged by size:
3351 1398 1184 429
AvrII C'CTAG_G
Cuts at: 2236 2236
Size: 6362
BamHI G'GATC_C
Cuts at: 1439 1787 3387 1439
Size: 348 1600 4414
Fragments arranged by size:
4414 1600 348
BanI G'GyrC_C
Cuts at: 122 1407 2248 2698 3378 3792 4040 4075 122
Size: 1285 841 450 680 414 248 35 2409
Fragments arranged by size:
2409 1285 841 680 450 414 248 35
BanII G_rGCy'C
Cuts at: 115 219 770 1002 2098 2542 3376 4406 115
Size: 104 551 232 1096 444 834 1030 2071
Fragments arranged by size:
2071 1096 1030 834 551 444 232 104
BbeI G_GCGC'C
Cuts at: 3796 4044 3796
Size: 248 6114
BbsI GAAGACnn'nnnn_
Cuts at: 2800 2800
Size: 6362
BbvCI CC'TCA_GC
Cuts at: 1943 2174 1943
Size: 231 6131
BciVI GTATCCnnnnn_n'
Cuts at: 1667 1828 1841 2325 3039 4260 5021 6362 1667
Size: 161 13 484 714 1221 761 1341 1667
Fragments arranged by size:
1667 1341 1221 761 714 484 161 13
BclI T'GATC_A
Cuts at: 2331 3882 2331
Size: 1551 4811
BfrBI ATG'CAT
Cuts at: 1762 1762
Size: 6362
BglII A'GATC_T
Cuts at: 3877 5914 3877
Size: 2037 4325
BlpI GC'TnA_GC
Cuts at: 232 890 3474 232
Size: 658 2584 3120
Fragments arranged by size:
3120 2584 658
Bme1580I G_kGCm'C
Cuts at: 115 770 1412 2542 3987 4080 5136 115
Size: 655 642 1130 1445 93 1056 1341
Fragments arranged by size:
1445 1341 1130 1056 655 642 93
BmrI ACTGGGnnnn_n'
Cuts at: 695 1110 1387 2875 3066 3574 3655 3990 6134
Size: 415 277 1488 191 508 81 335 2144
Cuts at: 6134 695
Size: 923
Fragments arranged by size:
2144 1488 923 508 415 335 277 191
81
BmtI G_CTAG'C
Cuts at: 2075 2075
Size: 6362
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4729 4729
Size: 6362
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 349 992 2015 2468 349
Size: 643 1023 453 4243
Fragments arranged by size:
4243 1023 643 453
Bpu10I CC'TnA_GC
Cuts at: 1670 1943 2174 1670
Size: 273 231 5858
Fragments arranged by size:
5858 273 231
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 635 3026 3538 4417 4909 5207 635
Size: 2391 512 879 492 298 1790
Fragments arranged by size:
2391 1790 879 512 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 6066 6066
Size: 6362
BsaAI yAC'GTr
Cuts at: 2274 3715 2274
Size: 1441 4921
BsaBI GATnn'nnATC
Cuts at: 6 1480 3170 3876 5913 6
Size: 1474 1690 706 2037 455
Fragments arranged by size:
2037 1690 1474 706 455
BsaHI Gr'CG_yC
Cuts at: 493 1171 2969 3793 4041 4743 6239 493
Size: 678 1798 824 248 702 1496 616
Fragments arranged by size:
1798 1496 824 702 678 616 248
BsaWI w'CCGG_w
Cuts at: 1486 2120 2289 3551 4072 4750 5024 5171 5582
Size: 634 169 1262 521 678 274 147 411
Cuts at: 5582 1486
Size: 2266
Fragments arranged by size:
2266 1262 678 634 521 411 274 169
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1299 1329 2067 2097 2622 2652 1299
Size: 30 738 30 525 30 5009
Fragments arranged by size:
5009 738 525 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 391 1424 1479 2162 2828 2998 391
Size: 1033 55 683 666 170 3755
Fragments arranged by size:
3755 1033 683 666 170 55
BseYI C'CCAG_C
Cuts at: 285 1412 2363 3787 4545 5122 5618 285
Size: 1127 951 1424 758 577 496 1029
Fragments arranged by size:
1424 1127 1029 951 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 439 827 1841 1904 2071 2474 439
Size: 388 1014 63 167 403 4327
Fragments arranged by size:
4327 1014 403 388 167 63
BsiEI CG_ry'CG
Cuts at: 104 3904 3950 5158 104
Size: 3800 46 1208 1308
Fragments arranged by size:
3800 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 344 1002 3042 3376 4154 4344 5136 344
Size: 658 2040 334 778 190 792 1570
Fragments arranged by size:
2040 1570 792 778 658 334 190
Bsp1286I G_dGCh'C
Cuts at: 115 219 344 770 1002 1412 2098 2542 3042
Size: 104 125 426 232 410 686 444 500
Cuts at: 3042 3376 3987 4080 4154 4344 4406 5136 115
Size: 334 611 93 74 190 62 730 1341
Fragments arranged by size:
1341 730 686 611 500 444 426 410
334 232 190 125 104 93 74 62
BspEI T'CCGG_A
Cuts at: 1486 2120 5582 1486
Size: 634 3462 2266
Fragments arranged by size:
3462 2266 634
BspHI T'CATG_A
Cuts at: 719 2591 5538 719
Size: 1872 2947 1543
Fragments arranged by size:
2947 1872 1543
BspMI ACCTGCnnnn'nnnn_
Cuts at: 609 716 2139 2236 3134 3412 3626 3928 4309
Size: 107 1423 97 898 278 214 302 381
Cuts at: 4309 609
Size: 2662
Fragments arranged by size:
2662 1423 898 381 302 278 214 107
97
BsrBI CCG'CTC
Cuts at: 50 2452 4654 4708 6192 6357 50
Size: 2402 2202 54 1484 165 55
Fragments arranged by size:
2402 2202 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 566 762 2342 2480 2650 4274 566
Size: 196 1580 138 170 1624 2654
Fragments arranged by size:
2654 1624 1580 196 170 138
BsrFI r'CCGG_y
Cuts at: 133 1824 4360 4541 4750 133
Size: 1691 2536 181 209 1745
Fragments arranged by size:
2536 1745 1691 209 181
BssSI C'ACGA_G
Cuts at: 1121 4633 4991 1121
Size: 3512 358 2492
Fragments arranged by size:
3512 2492 358
BstAPI GCAn_nnn'nTGC
Cuts at: 814 1768 3739 814
Size: 954 1971 3437
Fragments arranged by size:
3437 1971 954
BstBI TT'CG_AA
Cuts at: 4723 4723
Size: 6362
BstEII G'GTnAC_C
Cuts at: 621 2231 621
Size: 1610 4752
BstXI CCAn_nnnn'nTGG
Cuts at: 669 1512 1995 669
Size: 843 483 5036
Fragments arranged by size:
5036 843 483
BstYI r'GATC_y
Cuts at: 1 1294 1439 1787 3387 3854 3877 4212 4458
Size: 1293 145 348 1600 467 23 335 246
Cuts at: 4458 5470 5556 5568 5914 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1600 1293 1012 467 449 348 346 335
246 145 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 853 853
Size: 6362
BtgI C'CryG_G
Cuts at: 107 118 632 1517 2045 2104 2303 107
Size: 11 514 885 528 59 199 4166
Fragments arranged by size:
4166 885 528 514 199 59 11
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1748 2084 4312 4493 4656 1748
Size: 336 2228 181 163 3454
Fragments arranged by size:
3454 2228 336 181 163
BtsI GCAGTG_nn'
Cuts at: 473 506 1973 2795 3605 473
Size: 33 1467 822 810 3230
Fragments arranged by size:
3230 1467 822 810 33
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 444 479 2027 2062 444
Size: 35 1548 35 4744
Fragments arranged by size:
4744 1548 35 35
DraI TTT'AAA
Cuts at: 3361 3361
Size: 6362
DraIII CAC_nnn'GTG
Cuts at: 2698 2698
Size: 6362
DrdI GACnn_nn'nnGTC
Cuts at: 2974 4068 4926 2974
Size: 1094 858 4410
Fragments arranged by size:
4410 1094 858
EaeI y'GGCC_r
Cuts at: 934 1514 1997 2107 2282 3947 4121 4512 4539
Size: 580 483 110 175 1665 174 391 27
Cuts at: 4539 5884 934
Size: 1345 1412
Fragments arranged by size:
1665 1412 1345 580 483 391 175 174
110 27
EagI C'GGCC_G
Cuts at: 3947 3947
Size: 6362
EarI CTCTTCn'nnn_
Cuts at: 779 4385 4595 779
Size: 3606 210 2546
Fragments arranged by size:
3606 2546 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4570 4880 5026 6172 4570
Size: 310 146 1146 4760
Fragments arranged by size:
4760 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1000 3374 1000
Size: 2374 3988
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 344 349 641 992 1197 1262 1897 1946 1970
Size: 5 292 351 205 65 635 49 24
Cuts at: 1970 2015 2468 3313 4187 4619 5366 5634 344
Size: 45 453 845 874 432 747 268 1072
Fragments arranged by size:
1072 874 845 747 635 453 432 351
292 268 205 65 49 45 24 5
EcoNI CCTnn'n_nnAGG
Cuts at: 209 1163 2706 209
Size: 954 1543 3865
Fragments arranged by size:
3865 1543 954
EcoO109I rG'GnC_Cy
Cuts at: 112 240 404 767 1838 2539 3501 112
Size: 128 164 363 1071 701 962 2973
Fragments arranged by size:
2973 1071 962 701 363 164 128
EcoRI G'AATT_C
Cuts at: 1877 3366 1877
Size: 1489 4873
EcoRV GAT'ATC
Cuts at: 2589 2589
Size: 6362
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4381 4381
Size: 6362
FspI TGC'GCA
Cuts at: 4143 4143
Size: 6362
HaeII r_GCGC'y
Cuts at: 3796 4044 5066 6045 3796
Size: 248 1022 979 4113
Fragments arranged by size:
4113 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2299 2331 2776 2808 4204 4234 4236 4266 2299
Size: 32 445 32 1396 30 2 30 4395
Fragments arranged by size:
4395 1396 445 32 32 30 30 2
HincII GTy'rAC
Cuts at: 431 3401 431
Size: 2970 3392
HindIII A'AGCT_T
Cuts at: 653 653
Size: 6362
KasI G'GCGC_C
Cuts at: 3792 4040 3792
Size: 248 6114
KpnI G_GTAC'C
Cuts at: 3382 3382
Size: 6362
MfeI C'AATT_G
Cuts at: 1991 1991
Size: 6362
MluI A'CGCG_T
Cuts at: 5795 5795
Size: 6362
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1674 2380 2410 5033 5217 1674
Size: 706 30 2623 184 2819
Fragments arranged by size:
2819 2623 706 184 30
MscI TGG'CCA
Cuts at: 1516 1999 2284 4123 1516
Size: 483 285 1839 3755
Fragments arranged by size:
3755 1839 483 285
MslI CAynn'nnrTG
Cuts at: 667 1228 1993 3062 4351 4478 667
Size: 561 765 1069 1289 127 2551
Fragments arranged by size:
2551 1289 1069 765 561 127
MspA1I CmG'CkG
Cuts at: 120 289 811 870 1027 1291 2488 3084 3473
Size: 169 522 59 157 264 1197 596 389
Cuts at: 3473 3787 4147 5160 5405 120
Size: 314 360 1013 245 1077
Fragments arranged by size:
1197 1077 1013 596 522 389 360 314
264 245 169 157 59
NaeI GCC'GGC
Cuts at: 135 1826 4543 135
Size: 1691 2717 1954
Fragments arranged by size:
2717 1954 1691
NarI GG'CG_CC
Cuts at: 3793 4041 3793
Size: 248 6114
NcoI C'CATG_G
Cuts at: 107 632 1517 2303 107
Size: 525 885 786 4166
Fragments arranged by size:
4166 885 786 525
NgoMIV G'CCGG_C
Cuts at: 133 1824 4541 133
Size: 1691 2717 1954
Fragments arranged by size:
2717 1954 1691
NheI G'CTAG_C
Cuts at: 2071 2071
Size: 6362
NsiI A_TGCA'T
Cuts at: 1764 1764
Size: 6362
NspI r_CATG'y
Cuts at: 1036 1771 2737 3415 3915 4822 1036
Size: 735 966 678 500 907 2576
Fragments arranged by size:
2576 966 907 735 678 500
PasI CC'CwG_GG
Cuts at: 1812 1843 2091 1812
Size: 31 248 6083
Fragments arranged by size:
6083 248 31
PciI A'CATG_T
Cuts at: 4818 4818
Size: 6362
PfoI T'CCnGG_A
Cuts at: 2445 2445
Size: 6362
PmeI GTTT'AAAC
Cuts at: 3361 3361
Size: 6362
PmlI CAC'GTG
Cuts at: 2274 2274
Size: 6362
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5501 5533 5501
Size: 32 6330
PpuMI rG'GwC_Cy
Cuts at: 1838 1838
Size: 6362
PspOMI G'GGCC_C
Cuts at: 111 766 2538 111
Size: 655 1772 3935
Fragments arranged by size:
3935 1772 655
PspXI vC'TCGA_Gb
Cuts at: 800 2198 800
Size: 1398 4964
PstI C_TGCA'G
Cuts at: 1092 1239 2247 2610 3005 3131 3409 4094 1092
Size: 147 1008 363 395 126 278 685 3360
Fragments arranged by size:
3360 1008 685 395 363 278 147 126
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6362
PvuII CAG'CTG
Cuts at: 289 811 870 1291 2488 3084 3787 4147 289
Size: 522 59 421 1197 596 703 360 2504
Fragments arranged by size:
2504 1197 703 596 522 421 360 59
RsrII CG'GwC_CG
Cuts at: 4557 4557
Size: 6362
SacI G_AGCT'C
Cuts at: 1002 3376 1002
Size: 2374 3988
SacII CC_GC'GG
Cuts at: 121 121
Size: 6362
SalI G'TCGA_C
Cuts at: 429 3399 429
Size: 2970 3392
SanDI GG'GwC_CC
Cuts at: 1838 1838
Size: 6362
SapI GCTCTTCn'nnn_
Cuts at: 4385 4595 4385
Size: 210 6152
SbfI CC_TGCA'GG
Cuts at: 2247 3131 3409 2247
Size: 884 278 5200
Fragments arranged by size:
5200 884 278
ScaI AGT'ACT
Cuts at: 1218 5879 1218
Size: 4661 1701
SexAI A'CCwGG_T
Cuts at: 2694 2694
Size: 6362
SfcI C'TryA_G
Cuts at: 33 175 293 1088 1235 2243 2606 3001 3079
Size: 142 118 795 147 1008 363 395 78
Cuts at: 3079 3127 3405 4090 5083 5274 33
Size: 48 278 685 993 191 1121
Fragments arranged by size:
1121 1008 993 795 685 395 363 278
191 147 142 118 78 48
SfoI GGC'GCC
Cuts at: 3794 4042 3794
Size: 248 6114
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6362
SmaI CCC'GGG
Cuts at: 373 3384 373
Size: 3011 3351
SmlI C'TyrA_G
Cuts at: 650 800 2198 3041 3262 3517 4432 4924 5186
Size: 150 1398 843 221 255 915 492 262
Cuts at: 5186 650
Size: 1826
Fragments arranged by size:
1826 1398 915 843 492 262 255 221
150
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6362
SphI G_CATG'C
Cuts at: 1036 1771 3415 3915 1036
Size: 735 1644 500 3483
Fragments arranged by size:
3483 1644 735 500
SspI AAT'ATT
Cuts at: 1567 1567
Size: 6362
StuI AGG'CCT
Cuts at: 2241 2241
Size: 6362
StyI C'CwwG_G
Cuts at: 107 605 632 1400 1517 2236 2303 2707 2843
Size: 498 27 768 117 719 67 404 136
Cuts at: 2843 3496 3850 107
Size: 653 354 2619
Fragments arranged by size:
2619 768 719 653 498 404 354 136
117 67 27
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1815 2484 2503 3945 4078 4537 4746 1815
Size: 669 19 1442 133 459 209 3431
Fragments arranged by size:
3431 1442 669 459 209 133 19
TatI w'GTAC_w
Cuts at: 900 1216 5877 900
Size: 316 4661 1385
Fragments arranged by size:
4661 1385 316
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 334 366 1299 1331 2067 2099 2622 2654 5879
Size: 32 933 32 736 32 523 32 3225
Cuts at: 5879 5911 334
Size: 32 785
Fragments arranged by size:
3225 933 785 736 523 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 3286 4159 3286
Size: 873 5489
XbaI T'CTAG_A
Cuts at: 3093 3393 3093
Size: 300 6062
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2142 2808 3857 2142
Size: 666 1049 4647
Fragments arranged by size:
4647 1049 666
XhoI C'TCGA_G
Cuts at: 800 2198 800
Size: 1398 4964
XmaI C'CCGG_G
Cuts at: 371 3382 371
Size: 3011 3351
XmnI GAAnn'nnTTC
Cuts at: 1959 1959
Size: 6362
ZraI GAC'GTC
Cuts at: 2970 2970
Size: 6362
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AleI AloI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI
BbvCI BciVI BclI BfrBI BglII BlpI Bme1580I BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII HindIII KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NheI NsiI NspI PasI PciI PfoI PmeI
PmlI PpiI PpuMI PspOMI PspXI PstI PvuI PvuII
RsrII SacI SacII SalI SanDI SapI SbfI ScaI
SexAI SfcI SfoI SgfI SmaI SmlI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AhdI AlfI AscI BaeI BcgI BglI BmgBI
BsiWI BsmI BsmBI BsrGI BssHII BstZ17I ClaI FseI
FspAI HpaI NdeI NotI NruI PacI PflMI PshAI
PsiI PsrI SfiI SgrAI SnaBI SrfI SwaI