Restriction map for
pF1KB5996
(Circular) (Six-base) MAPSORT of: pF1KB5996.seq Check: 4455 from: 1 to: 4952
pF1KB5996 4952 bp
With 182 enzymes: SgfI *
June 26, 2008 10:45 ..
AccI GT'mk_AC
Cuts at: 1990 1990
Size: 4952
Acc65I G'GTAC_C
Cuts at: 1968 1968
Size: 4952
AclI AA'CG_TT
Cuts at: 4792 4792
Size: 4952
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 887 1219 2777 3209 3956 4224 887
Size: 332 1558 432 747 268 1615
Fragments arranged by size:
1615 1558 747 432 332 268
AflIII A'CryG_T
Cuts at: 259 1799 2304 3408 4385 259
Size: 1540 505 1104 977 826
Fragments arranged by size:
1540 1104 977 826 505
AgeI A'CCGG_T
Cuts at: 3340 3340
Size: 4952
AleI CACnn'nnGTG
Cuts at: 1516 1516
Size: 4952
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1211 1211
Size: 4952
AlwNI CAG_nnn'CTG
Cuts at: 354 2257 3824 354
Size: 1903 1567 1482
Fragments arranged by size:
1903 1567 1482
ApaI G_GGCC'C
Cuts at: 1632 1632
Size: 4952
ApaLI G'TGCA_C
Cuts at: 173 3722 173
Size: 3549 1403
ApoI r'AATT_y
Cuts at: 1956 4305 4561 1956
Size: 2349 256 2347
Fragments arranged by size:
2349 2347 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4952
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4952
AvaI C'yCGr_G
Cuts at: 129 1972 129
Size: 1843 3109
AvrII C'CTAG_G
Cuts at: 639 639
Size: 4952
BamHI G'GATC_C
Cuts at: 1977 1977
Size: 4952
BanI G'GyrC_C
Cuts at: 116 302 423 456 512 643 1084 1968 2382
Size: 186 121 33 56 131 441 884 414
Cuts at: 2382 2630 2665 116
Size: 248 35 2403
Fragments arranged by size:
2403 884 441 414 248 186 131 121
56 35 33
BanII G_rGCy'C
Cuts at: 619 850 998 1133 1159 1315 1412 1632 1733
Size: 231 148 135 26 156 97 220 101
Cuts at: 1733 1790 1966 2996 619
Size: 57 176 1030 2575
Fragments arranged by size:
2575 1030 231 220 176 156 148 135
101 97 57 26
BbeI G_GCGC'C
Cuts at: 1088 2386 2634 1088
Size: 1298 248 3406
Fragments arranged by size:
3406 1298 248
BbsI GAAGACnn'nnnn_
Cuts at: 415 1919 415
Size: 1504 3448
BbvCI CC'TCA_GC
Cuts at: 308 496 308
Size: 188 4764
BciVI GTATCCnnnnn_n'
Cuts at: 2850 3611 4952 2850
Size: 761 1341 2850
Fragments arranged by size:
2850 1341 761
BclI T'GATC_A
Cuts at: 2472 2472
Size: 4952
BglI GCCn_nnn'nGGC
Cuts at: 1100 1288 1636 1100
Size: 188 348 4416
Fragments arranged by size:
4416 348 188
BglII A'GATC_T
Cuts at: 1140 2467 4504 1140
Size: 1327 2037 1588
Fragments arranged by size:
2037 1588 1327
BlpI GC'TnA_GC
Cuts at: 270 1648 1774 2064 270
Size: 1378 126 290 3158
Fragments arranged by size:
3158 1378 290 126
Bme1580I G_kGCm'C
Cuts at: 177 480 648 1284 1632 2577 2670 3726 177
Size: 303 168 636 348 945 93 1056 1403
Fragments arranged by size:
1403 1056 945 636 348 303 168 93
BmrI ACTGGGnnnn_n'
Cuts at: 442 2164 2245 2580 4724 442
Size: 1722 81 335 2144 670
Fragments arranged by size:
2144 1722 670 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 323 1152 1695 3319 323
Size: 829 543 1624 1956
Fragments arranged by size:
1956 1624 829 543
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 262 385 552 706 736 908 976 1203 1633
Size: 123 167 154 30 172 68 227 430
Cuts at: 1633 262
Size: 3581
Fragments arranged by size:
3581 430 227 172 167 154 123 68
30
Bpu10I CC'TnA_GC
Cuts at: 308 496 1522 308
Size: 188 1026 3738
Fragments arranged by size:
3738 1026 188
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 682 834 1291 2128 3007 3499 3797 682
Size: 152 457 837 879 492 298 1837
Fragments arranged by size:
1837 879 837 492 457 298 152
BsaI GGTCTCn'nnnn_
Cuts at: 1576 4656 1576
Size: 3080 1872
BsaAI yAC'GTr
Cuts at: 1518 2305 1518
Size: 787 4165
BsaBI GATnn'nnATC
Cuts at: 6 2466 4503 6
Size: 2460 2037 455
Fragments arranged by size:
2460 2037 455
BsaHI Gr'CG_yC
Cuts at: 1085 2383 2631 3333 4829 1085
Size: 1298 248 702 1496 1208
Fragments arranged by size:
1496 1298 1208 702 248
BsaWI w'CCGG_w
Cuts at: 1589 2141 2662 3340 3614 3761 4172 1589
Size: 552 521 678 274 147 411 2369
Fragments arranged by size:
2369 678 552 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 922 952 922
Size: 30 4922
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1271 1420 1630 1271
Size: 149 210 4593
Fragments arranged by size:
4593 210 149
BseYI C'CCAG_C
Cuts at: 418 490 780 843 1427 1488 2377 3135 3712
Size: 72 290 63 584 61 889 758 577
Cuts at: 3712 4208 418
Size: 496 1162
Fragments arranged by size:
1162 889 758 584 577 496 290 72
63 61
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 189 202 460 519 1705 1794 189
Size: 13 258 59 1186 89 3347
Fragments arranged by size:
3347 1186 258 89 59 13
BsiEI CG_ry'CG
Cuts at: 104 526 1104 2494 2540 3748 104
Size: 422 578 1390 46 1208 1308
Fragments arranged by size:
1390 1308 1208 578 422 46
BsiHKAI G_wGCw'C
Cuts at: 177 1412 1523 1966 2744 2934 3726 177
Size: 1235 111 443 778 190 792 1403
Fragments arranged by size:
1403 1235 792 778 443 190 111
Bsp1286I G_dGCh'C
Cuts at: 177 480 619 648 850 998 1133 1159 1284
Size: 303 139 29 202 148 135 26 125
Cuts at: 1284 1315 1412 1523 1632 1733 1790 1966 2577
Size: 31 97 111 109 101 57 176 611
Cuts at: 2577 2670 2744 2934 2996 3726 177
Size: 93 74 190 62 730 1403
Fragments arranged by size:
1403 730 611 303 202 190 176 148
139 135 125 111 109 101 97 93
74 62 57 31 29 26
BspEI T'CCGG_A
Cuts at: 4172 4172
Size: 4952
BspHI T'CATG_A
Cuts at: 4128 4128
Size: 4952
BspMI ACCTGCnnnn'nnnn_
Cuts at: 977 1319 2002 2216 2518 2899 977
Size: 342 683 214 302 381 3030
Fragments arranged by size:
3030 683 381 342 302 214
BsrBI CCG'CTC
Cuts at: 50 987 1739 3244 3298 4782 4947 50
Size: 937 752 1505 54 1484 165 55
Fragments arranged by size:
1505 1484 937 752 165 55 54
BsrDI GCAATG_nn'
Cuts at: 655 2864 655
Size: 2209 2743
BsrFI r'CCGG_y
Cuts at: 509 604 1766 1813 2950 3131 3340 509
Size: 95 1162 47 1137 181 209 2121
Fragments arranged by size:
2121 1162 1137 209 181 95 47
BssSI C'ACGA_G
Cuts at: 3223 3581 3223
Size: 358 4594
BstAPI GCAn_nnn'nTGC
Cuts at: 2329 2329
Size: 4952
BstBI TT'CG_AA
Cuts at: 1466 3313 1466
Size: 1847 3105
BstEII G'GTnAC_C
Cuts at: 133 133
Size: 4952
BstXI CCAn_nnnn'nTGG
Cuts at: 1125 1848 1125
Size: 723 4229
BstYI r'GATC_y
Cuts at: 1 1140 1977 2444 2467 2802 3048 4060 4146
Size: 1139 837 467 23 335 246 1012 86
Cuts at: 4146 4158 4504 1
Size: 12 346 449
Fragments arranged by size:
1139 1012 837 467 449 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 1080 107
Size: 973 3979
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 985 2902 3083 3246 985
Size: 1917 181 163 2691
Fragments arranged by size:
2691 1917 181 163
BtsI GCAGTG_nn'
Cuts at: 177 1089 1659 1706 2195 177
Size: 912 570 47 489 2934
Fragments arranged by size:
2934 912 570 489 47
DraI TTT'AAA
Cuts at: 1951 1951
Size: 4952
DrdI GACnn_nn'nnGTC
Cuts at: 2658 3516 2658
Size: 858 4094
EaeI y'GGCC_r
Cuts at: 110 140 217 318 523 602 1101 1289 2537
Size: 30 77 101 205 79 499 188 1248
Cuts at: 2537 2711 3102 3129 4474 110
Size: 174 391 27 1345 588
Fragments arranged by size:
1345 1248 588 499 391 205 188 174
101 79 77 30 27
EagI C'GGCC_G
Cuts at: 523 1101 2537 523
Size: 578 1436 2938
Fragments arranged by size:
2938 1436 578
EarI CTCTTCn'nnn_
Cuts at: 1039 2975 3185 1039
Size: 1936 210 2806
Fragments arranged by size:
2806 1936 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3160 3470 3616 4762 3160
Size: 310 146 1146 3350
Fragments arranged by size:
3350 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1410 1964 1410
Size: 554 4398
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 262 385 552 706 736 887 908 976 1203
Size: 123 167 154 30 151 21 68 227
Cuts at: 1203 1219 1633 2777 3209 3956 4224 262
Size: 16 414 1144 432 747 268 990
Fragments arranged by size:
1144 990 747 432 414 268 227 167
154 151 123 68 30 21 16
EcoNI CCTnn'n_nnAGG
Cuts at: 1402 1402
Size: 4952
EcoO109I rG'GnC_Cy
Cuts at: 351 444 1473 1527 1628 2091 351
Size: 93 1029 54 101 463 3212
Fragments arranged by size:
3212 1029 463 101 93 54
EcoRI G'AATT_C
Cuts at: 1956 1956
Size: 4952
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2971 2971
Size: 4952
FspI TGC'GCA
Cuts at: 2733 2733
Size: 4952
HaeII r_GCGC'y
Cuts at: 1088 2386 2634 3656 4635 1088
Size: 1298 248 1022 979 1405
Fragments arranged by size:
1405 1298 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 922 954 1289 1321 2794 2824 2826 2856 922
Size: 32 335 32 1473 30 2 30 3018
Fragments arranged by size:
3018 1473 335 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1991 1991
Size: 4952
KasI G'GCGC_C
Cuts at: 1084 2382 2630 1084
Size: 1298 248 3406
Fragments arranged by size:
3406 1298 248
KpnI G_GTAC'C
Cuts at: 1972 1972
Size: 4952
MluI A'CGCG_T
Cuts at: 4385 4385
Size: 4952
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 605 1415 3623 3807 605
Size: 810 2208 184 1750
Fragments arranged by size:
2208 1750 810 184
MscI TGG'CCA
Cuts at: 112 320 1291 2713 112
Size: 208 971 1422 2351
Fragments arranged by size:
2351 1422 971 208
MslI CAynn'nnrTG
Cuts at: 258 1516 1816 2941 3068 258
Size: 1258 300 1125 127 2142
Fragments arranged by size:
2142 1258 1125 300 127
MspA1I CmG'CkG
Cuts at: 186 714 954 1350 1488 2063 2377 2737 3750
Size: 528 240 396 138 575 314 360 1013
Cuts at: 3750 3995 186
Size: 245 1143
Fragments arranged by size:
1143 1013 575 528 396 360 314 245
240 138
NaeI GCC'GGC
Cuts at: 606 1768 3133 606
Size: 1162 1365 2425
Fragments arranged by size:
2425 1365 1162
NarI GG'CG_CC
Cuts at: 1085 2383 2631 1085
Size: 1298 248 3406
Fragments arranged by size:
3406 1298 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 4952
NgoMIV G'CCGG_C
Cuts at: 604 1766 3131 604
Size: 1162 1365 2425
Fragments arranged by size:
2425 1365 1162
NspI r_CATG'y
Cuts at: 263 484 1380 1803 2005 2505 3412 263
Size: 221 896 423 202 500 907 1803
Fragments arranged by size:
1803 907 896 500 423 221 202
PasI CC'CwG_GG
Cuts at: 1536 1536
Size: 4952
PciI A'CATG_T
Cuts at: 259 1799 3408 259
Size: 1540 1609 1803
Fragments arranged by size:
1803 1609 1540
PflMI CCAn_nnn'nTGG
Cuts at: 327 354 466 1068 327
Size: 27 112 602 4211
Fragments arranged by size:
4211 602 112 27
PfoI T'CCnGG_A
Cuts at: 353 353
Size: 4952
PmeI GTTT'AAAC
Cuts at: 1951 1951
Size: 4952
PmlI CAC'GTG
Cuts at: 1518 1518
Size: 4952
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1885 1917 4091 4123 1885
Size: 32 2174 32 2714
Fragments arranged by size:
2714 2174 32 32
PpuMI rG'GwC_Cy
Cuts at: 351 444 1473 1527 351
Size: 93 1029 54 3776
Fragments arranged by size:
3776 1029 93 54
PshAI GACnn'nnGTC
Cuts at: 1103 1103
Size: 4952
PspOMI G'GGCC_C
Cuts at: 1628 1628
Size: 4952
PstI C_TGCA'G
Cuts at: 1321 1666 1999 2684 1321
Size: 345 333 685 3589
Fragments arranged by size:
3589 685 345 333
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4952
PvuII CAG'CTG
Cuts at: 186 714 954 1488 2377 2737 186
Size: 528 240 534 889 360 2401
Fragments arranged by size:
2401 889 534 528 360 240
RsrII CG'GwC_CG
Cuts at: 3147 3147
Size: 4952
SacI G_AGCT'C
Cuts at: 1412 1966 1412
Size: 554 4398
SalI G'TCGA_C
Cuts at: 1989 1989
Size: 4952
SapI GCTCTTCn'nnn_
Cuts at: 2975 3185 2975
Size: 210 4742
SbfI CC_TGCA'GG
Cuts at: 1999 1999
Size: 4952
ScaI AGT'ACT
Cuts at: 4469 4469
Size: 4952
SfcI C'TryA_G
Cuts at: 33 709 1317 1662 1995 2680 3673 3864 33
Size: 676 608 345 333 685 993 191 1121
Fragments arranged by size:
1121 993 685 676 608 345 333 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 1636 1636
Size: 4952
SfoI GGC'GCC
Cuts at: 1086 2384 2632 1086
Size: 1298 248 3406
Fragments arranged by size:
3406 1298 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4952
SmaI CCC'GGG
Cuts at: 1974 1974
Size: 4952
SmlI C'TyrA_G
Cuts at: 697 849 1306 2107 3022 3514 3776 697
Size: 152 457 801 915 492 262 1873
Fragments arranged by size:
1873 915 801 492 457 262 152
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4952
SphI G_CATG'C
Cuts at: 2005 2505 2005
Size: 500 4452
StuI AGG'CCT
Cuts at: 734 885 1639 734
Size: 151 754 4047
Fragments arranged by size:
4047 754 151
StyI C'CwwG_G
Cuts at: 107 639 648 1442 1841 2086 2440 107
Size: 532 9 794 399 245 354 2619
Fragments arranged by size:
2619 794 532 399 354 245 9
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 263 1528 1717 2535 2668 3127 3336 263
Size: 1265 189 818 133 459 209 1879
Fragments arranged by size:
1879 1265 818 459 209 189 133
TatI w'GTAC_w
Cuts at: 4467 4467
Size: 4952
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 238 270 4469 4501 238
Size: 32 4199 32 689
Fragments arranged by size:
4199 689 32 32
Tth111I GACn'n_nGTC
Cuts at: 2749 2749
Size: 4952
XbaI T'CTAG_A
Cuts at: 1983 1983
Size: 4952
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 198 2447 198
Size: 2249 2703
XmaI C'CCGG_G
Cuts at: 1972 1972
Size: 4952
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlfI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII
BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI
BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PspOMI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfiI SfoI SgfI SmaI
SmlI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AloI AscI BaeI
BcgI BfrBI BmgBI BmtI BsiWI BsmI BsmBI BsrGI
BssHII BstZ17I Bsu36I ClaI CspCI DraIII EcoRV FseI
FspAI HindIII HpaI MfeI NdeI NheI NotI NruI
NsiI PacI PsiI PspXI PsrI SacII SanDI SexAI
SgrAI SnaBI SrfI SspI SwaI XhoI XmnI ZraI