Restriction map for
pF1KB5992
(Circular) (Six-base) MAPSORT of: pF1KB5992.seq Check: 8816 from: 1 to: 4940
pF1KB5992 4940 bp
With 182 enzymes: SgfI *
July 10, 2012 13:27 ..
AccI GT'mk_AC
Cuts at: 1978 1978
Size: 4940
Acc65I G'GTAC_C
Cuts at: 1956 1956
Size: 4940
AclI AA'CG_TT
Cuts at: 4780 4780
Size: 4940
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 341 1097 1121 1626 1907 2765 3197 3944 4212
Size: 756 24 505 281 858 432 747 268
Cuts at: 4212 341
Size: 1069
Fragments arranged by size:
1069 858 756 747 505 432 281 268
24
AfeI AGC'GCT
Cuts at: 1739 1739
Size: 4940
AflIII A'CryG_T
Cuts at: 2292 3396 4373 2292
Size: 1104 977 2859
Fragments arranged by size:
2859 1104 977
AgeI A'CCGG_T
Cuts at: 3328 3328
Size: 4940
AhdI GACnn_n'nnGTC
Cuts at: 990 990
Size: 4940
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 723 723
Size: 4940
AlwNI CAG_nnn'CTG
Cuts at: 538 2245 3812 538
Size: 1707 1567 1666
Fragments arranged by size:
1707 1666 1567
ApaLI G'TGCA_C
Cuts at: 3710 3710
Size: 4940
ApoI r'AATT_y
Cuts at: 147 416 589 792 1944 4293 4549 147
Size: 269 173 203 1152 2349 256 538
Fragments arranged by size:
2349 1152 538 269 256 203 173
AseI AT'TA_AT
Cuts at: 20 20
Size: 4940
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4940
AvaI C'yCGr_G
Cuts at: 1960 1960
Size: 4940
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1545 1578 1545
Size: 33 4907
BamHI G'GATC_C
Cuts at: 1965 1965
Size: 4940
BanI G'GyrC_C
Cuts at: 1956 2370 2618 2653 1956
Size: 414 248 35 4243
Fragments arranged by size:
4243 414 248 35
BanII G_rGCy'C
Cuts at: 1954 2984 1954
Size: 1030 3910
BbeI G_GCGC'C
Cuts at: 2374 2622 2374
Size: 248 4692
BbsI GAAGACnn'nnnn_
Cuts at: 347 474 572 1194 347
Size: 127 98 622 4093
Fragments arranged by size:
4093 622 127 98
BbvCI CC'TCA_GC
Cuts at: 1196 1196
Size: 4940
BciVI GTATCCnnnnn_n'
Cuts at: 1228 2838 3599 4940 1228
Size: 1610 761 1341 1228
Fragments arranged by size:
1610 1341 1228 761
BclI T'GATC_A
Cuts at: 2460 2460
Size: 4940
BfrBI ATG'CAT
Cuts at: 316 775 316
Size: 459 4481
BglII A'GATC_T
Cuts at: 2455 4492 2455
Size: 2037 2903
BlpI GC'TnA_GC
Cuts at: 2052 2052
Size: 4940
Bme1580I G_kGCm'C
Cuts at: 2565 2658 3714 2565
Size: 93 1056 3791
Fragments arranged by size:
3791 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 258 2152 2233 2568 4712 258
Size: 1894 81 335 2144 486
Fragments arranged by size:
2144 1894 486 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3307 3307
Size: 4940
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 362 488 1565 362
Size: 126 1077 3737
Fragments arranged by size:
3737 1077 126
Bpu10I CC'TnA_GC
Cuts at: 1196 1727 1196
Size: 531 4409
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1831 2116 2995 3487 3785 1831
Size: 285 879 492 298 2986
Fragments arranged by size:
2986 879 492 298 285
BsaI GGTCTCn'nnnn_
Cuts at: 4644 4644
Size: 4940
BsaAI yAC'GTr
Cuts at: 2293 2293
Size: 4940
BsaBI GATnn'nnATC
Cuts at: 6 1453 2454 4491 6
Size: 1447 1001 2037 455
Fragments arranged by size:
2037 1447 1001 455
BsaHI Gr'CG_yC
Cuts at: 2371 2619 3321 4817 2371
Size: 248 702 1496 2494
Fragments arranged by size:
2494 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2129 2650 3328 3602 3749 4160 2129
Size: 521 678 274 147 411 2909
Fragments arranged by size:
2909 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 935 935
Size: 4940
BseYI C'CCAG_C
Cuts at: 1427 2365 3123 3700 4196 1427
Size: 938 758 577 496 2171
Fragments arranged by size:
2171 938 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 800 1593 800
Size: 793 4147
BsiEI CG_ry'CG
Cuts at: 104 2482 2528 3736 104
Size: 2378 46 1208 1308
Fragments arranged by size:
2378 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 911 1954 2732 2922 3714 911
Size: 1043 778 190 792 2137
Fragments arranged by size:
2137 1043 792 778 190
BsmI GAATG_Cn'
Cuts at: 712 1015 1288 1681 712
Size: 303 273 393 3971
Fragments arranged by size:
3971 393 303 273
Bsp1286I G_dGCh'C
Cuts at: 911 1954 2565 2658 2732 2922 2984 3714 911
Size: 1043 611 93 74 190 62 730 2137
Fragments arranged by size:
2137 1043 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 4160 4160
Size: 4940
BspHI T'CATG_A
Cuts at: 581 1173 4116 581
Size: 592 2943 1405
Fragments arranged by size:
2943 1405 592
BspMI ACCTGCnnnn'nnnn_
Cuts at: 390 1990 2204 2506 2887 390
Size: 1600 214 302 381 2443
Fragments arranged by size:
2443 1600 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1534 3232 3286 4770 4935 50
Size: 1484 1698 54 1484 165 55
Fragments arranged by size:
1698 1484 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1820 2852 1820
Size: 1032 3908
BsrFI r'CCGG_y
Cuts at: 2938 3119 3328 2938
Size: 181 209 4550
Fragments arranged by size:
4550 209 181
BsrGI T'GTAC_A
Cuts at: 900 900
Size: 4940
BssSI C'ACGA_G
Cuts at: 1916 3211 3569 1916
Size: 1295 358 3287
Fragments arranged by size:
3287 1295 358
BstAPI GCAn_nnn'nTGC
Cuts at: 322 2317 322
Size: 1995 2945
BstBI TT'CG_AA
Cuts at: 3301 3301
Size: 4940
BstXI CCAn_nnnn'nTGG
Cuts at: 1467 1467
Size: 4940
BstYI r'GATC_y
Cuts at: 1 1713 1965 2432 2455 2790 3036 4048 4134
Size: 1712 252 467 23 335 246 1012 86
Cuts at: 4134 4146 4492 1
Size: 12 346 449
Fragments arranged by size:
1712 1012 467 449 346 335 252 246
86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2890 3071 3234 2890
Size: 181 163 4596
Fragments arranged by size:
4596 181 163
BtsI GCAGTG_nn'
Cuts at: 166 2183 166
Size: 2017 2923
ClaI AT'CG_AT
Cuts at: 511 511
Size: 4940
DraI TTT'AAA
Cuts at: 1939 1939
Size: 4940
DrdI GACnn_nn'nnGTC
Cuts at: 2646 3504 2646
Size: 858 4082
EaeI y'GGCC_r
Cuts at: 1451 1599 2525 2699 3090 3117 4462 1451
Size: 148 926 174 391 27 1345 1929
Fragments arranged by size:
1929 1345 926 391 174 148 27
EagI C'GGCC_G
Cuts at: 2525 2525
Size: 4940
EarI CTCTTCn'nnn_
Cuts at: 455 1366 1883 2963 3173 455
Size: 911 517 1080 210 2222
Fragments arranged by size:
2222 1080 911 517 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3148 3458 3604 4750 3148
Size: 310 146 1146 3338
Fragments arranged by size:
3338 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1952 1952
Size: 4940
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 341 362 488 1097 1121 1565 1626 1907 2765
Size: 21 126 609 24 444 61 281 858
Cuts at: 2765 3197 3944 4212 341
Size: 432 747 268 1069
Fragments arranged by size:
1069 858 747 609 444 432 281 268
126 61 24 21
EcoO109I rG'GnC_Cy
Cuts at: 800 2079 800
Size: 1279 3661
EcoRI G'AATT_C
Cuts at: 1944 1944
Size: 4940
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2959 2959
Size: 4940
FspI TGC'GCA
Cuts at: 2721 2721
Size: 4940
HaeII r_GCGC'y
Cuts at: 1741 2374 2622 3644 4623 1741
Size: 633 248 1022 979 2058
Fragments arranged by size:
2058 1022 979 633 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 337 369 976 1008 2782 2812 2814 2844 337
Size: 32 607 32 1774 30 2 30 2433
Fragments arranged by size:
2433 1774 607 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1979 1979
Size: 4940
KasI G'GCGC_C
Cuts at: 2370 2618 2370
Size: 248 4692
KpnI G_GTAC'C
Cuts at: 1960 1960
Size: 4940
MfeI C'AATT_G
Cuts at: 326 326
Size: 4940
MluI A'CGCG_T
Cuts at: 4373 4373
Size: 4940
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1421 1823 3611 3795 1421
Size: 402 1788 184 2566
Fragments arranged by size:
2566 1788 402 184
MscI TGG'CCA
Cuts at: 1453 1601 2701 1453
Size: 148 1100 3692
Fragments arranged by size:
3692 1100 148
MslI CAynn'nnrTG
Cuts at: 176 776 902 2929 3056 176
Size: 600 126 2027 127 2060
Fragments arranged by size:
2060 2027 600 127 126
MspA1I CmG'CkG
Cuts at: 1431 2051 2365 2725 3738 3983 1431
Size: 620 314 360 1013 245 2388
Fragments arranged by size:
2388 1013 620 360 314 245
NaeI GCC'GGC
Cuts at: 3121 3121
Size: 4940
NarI GG'CG_CC
Cuts at: 2371 2619 2371
Size: 248 4692
NdeI CA'TA_TG
Cuts at: 558 970 1620 1668 558
Size: 412 650 48 3830
Fragments arranged by size:
3830 650 412 48
NgoMIV G'CCGG_C
Cuts at: 3119 3119
Size: 4940
NsiI A_TGCA'T
Cuts at: 318 777 318
Size: 459 4481
NspI r_CATG'y
Cuts at: 775 779 1993 2493 3400 775
Size: 4 1214 500 907 2315
Fragments arranged by size:
2315 1214 907 500 4
PciI A'CATG_T
Cuts at: 3396 3396
Size: 4940
PflMI CCAn_nnn'nTGG
Cuts at: 113 1338 113
Size: 1225 3715
PfoI T'CCnGG_A
Cuts at: 884 1322 884
Size: 438 4502
PmeI GTTT'AAAC
Cuts at: 1939 1939
Size: 4940
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4079 4111 4079
Size: 32 4908
PpuMI rG'GwC_Cy
Cuts at: 800 800
Size: 4940
PstI C_TGCA'G
Cuts at: 225 451 944 1987 2672 225
Size: 226 493 1043 685 2493
Fragments arranged by size:
2493 1043 685 493 226
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4940
PvuII CAG'CTG
Cuts at: 1431 2365 2725 1431
Size: 934 360 3646
Fragments arranged by size:
3646 934 360
RsrII CG'GwC_CG
Cuts at: 3135 3135
Size: 4940
SacI G_AGCT'C
Cuts at: 1954 1954
Size: 4940
SalI G'TCGA_C
Cuts at: 1977 1977
Size: 4940
SanDI GG'GwC_CC
Cuts at: 800 800
Size: 4940
SapI GCTCTTCn'nnn_
Cuts at: 2963 3173 2963
Size: 210 4730
SbfI CC_TGCA'GG
Cuts at: 1987 1987
Size: 4940
ScaI AGT'ACT
Cuts at: 4457 4457
Size: 4940
SfcI C'TryA_G
Cuts at: 33 221 447 940 1983 2668 3661 3852 33
Size: 188 226 493 1043 685 993 191 1121
Fragments arranged by size:
1121 1043 993 685 493 226 191 188
SfoI GGC'GCC
Cuts at: 2372 2620 2372
Size: 248 4692
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4940
SmaI CCC'GGG
Cuts at: 1962 1962
Size: 4940
SmlI C'TyrA_G
Cuts at: 1810 2095 3010 3502 3764 1810
Size: 285 915 492 262 2986
Fragments arranged by size:
2986 915 492 285 262
SpeI A'CTAG_T
Cuts at: 67 879 67
Size: 812 4128
SphI G_CATG'C
Cuts at: 1993 2493 1993
Size: 500 4440
SspI AAT'ATT
Cuts at: 1180 1381 1180
Size: 201 4739
StuI AGG'CCT
Cuts at: 1838 1838
Size: 4940
StyI C'CwwG_G
Cuts at: 1857 2074 2428 1857
Size: 217 354 4369
Fragments arranged by size:
4369 354 217
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 105 2523 2656 3115 3324 105
Size: 2418 133 459 209 1721
Fragments arranged by size:
2418 1721 459 209 133
TatI w'GTAC_w
Cuts at: 217 900 4455 217
Size: 683 3555 702
Fragments arranged by size:
3555 702 683
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4457 4489 4457
Size: 32 4908
Tth111I GACn'n_nGTC
Cuts at: 1437 2737 1437
Size: 1300 3640
XbaI T'CTAG_A
Cuts at: 1971 1971
Size: 4940
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 114 2435 114
Size: 2321 2619
XmaI C'CCGG_G
Cuts at: 1960 1960
Size: 4940
XmnI GAAnn'nnTTC
Cuts at: 192 589 1258 192
Size: 397 669 3874
Fragments arranged by size:
3874 669 397
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AhdI
AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI
BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI
BfrBI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstXI
BstYI BtgZI BtsI ClaI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NdeI NgoMIV NsiI
NspI PciI PflMI PfoI PmeI PpiI PpuMI PstI
PvuI PvuII RsrII SacI SalI SanDI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AflII AleI AloI ApaI AscI AvrII
BcgI BglI BmgBI BmtI BsaXI BsiWI BsmBI BssHII
BstEII BstZ17I Bsu36I BtgI CspCI DraIII EcoNI EcoRV
FseI FspAI HindIII HpaI NcoI NheI NotI NruI
PacI PasI PmlI PshAI PsiI PspOMI PspXI PsrI
SacII SexAI SfiI SgrAI SnaBI SrfI SwaI XhoI
ZraI