Restriction map for
pF1KB5987
(Circular) (Six-base) MAPSORT of: pF1KB5987.seq Check: 7446 from: 1 to: 4823
pF1KB5987 4823 bp
With 182 enzymes: SgfI *
October 20, 2011 13:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1725 1725
Size: 4823
AccI GT'mk_AC
Cuts at: 1861 1861
Size: 4823
Acc65I G'GTAC_C
Cuts at: 1839 1839
Size: 4823
AclI AA'CG_TT
Cuts at: 4663 4663
Size: 4823
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1669 2648 3080 3827 4095 1669
Size: 979 432 747 268 2397
Fragments arranged by size:
2397 979 747 432 268
AflIII A'CryG_T
Cuts at: 739 2175 3279 4256 739
Size: 1436 1104 977 1306
Fragments arranged by size:
1436 1306 1104 977
AgeI A'CCGG_T
Cuts at: 3211 3211
Size: 4823
AleI CACnn'nnGTG
Cuts at: 117 117
Size: 4823
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 179 179
Size: 4823
AlwNI CAG_nnn'CTG
Cuts at: 1721 2128 3695 1721
Size: 407 1567 2849
Fragments arranged by size:
2849 1567 407
ApaLI G'TGCA_C
Cuts at: 3593 3593
Size: 4823
ApoI r'AATT_y
Cuts at: 271 663 1363 1402 1827 4176 4432 271
Size: 392 700 39 425 2349 256 662
Fragments arranged by size:
2349 700 662 425 392 256 39
AseI AT'TA_AT
Cuts at: 20 20
Size: 4823
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4823
AvaI C'yCGr_G
Cuts at: 370 1583 1843 370
Size: 1213 260 3350
Fragments arranged by size:
3350 1213 260
BamHI G'GATC_C
Cuts at: 1848 1848
Size: 4823
BanI G'GyrC_C
Cuts at: 1839 2253 2501 2536 1839
Size: 414 248 35 4126
Fragments arranged by size:
4126 414 248 35
BanII G_rGCy'C
Cuts at: 1281 1556 1837 2867 1281
Size: 275 281 1030 3237
Fragments arranged by size:
3237 1030 281 275
BbeI G_GCGC'C
Cuts at: 2257 2505 2257
Size: 248 4575
BciVI GTATCCnnnnn_n'
Cuts at: 1344 1402 1715 2721 3482 4823 1344
Size: 58 313 1006 761 1341 1344
Fragments arranged by size:
1344 1341 1006 761 313 58
BclI T'GATC_A
Cuts at: 855 2343 855
Size: 1488 3335
BglII A'GATC_T
Cuts at: 2338 4375 2338
Size: 2037 2786
BlpI GC'TnA_GC
Cuts at: 1935 1935
Size: 4823
Bme1580I G_kGCm'C
Cuts at: 250 833 2448 2541 3597 250
Size: 583 1615 93 1056 1476
Fragments arranged by size:
1615 1476 1056 583 93
BmrI ACTGGGnnnn_n'
Cuts at: 195 535 2035 2116 2451 4595 195
Size: 340 1500 81 335 2144 423
Fragments arranged by size:
2144 1500 423 340 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3190 3190
Size: 4823
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1513 1570 1513
Size: 57 4766
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 364 717 1069 1540 1999 2878 3370 3668 364
Size: 353 352 471 459 879 492 298 1519
Fragments arranged by size:
1519 879 492 471 459 353 352 298
BsaI GGTCTCn'nnnn_
Cuts at: 4527 4527
Size: 4823
BsaAI yAC'GTr
Cuts at: 1524 2176 1524
Size: 652 4171
BsaBI GATnn'nnATC
Cuts at: 6 2337 4374 6
Size: 2331 2037 455
Fragments arranged by size:
2331 2037 455
BsaHI Gr'CG_yC
Cuts at: 1494 2254 2502 3204 4700 1494
Size: 760 248 702 1496 1617
Fragments arranged by size:
1617 1496 760 702 248
BsaWI w'CCGG_w
Cuts at: 2012 2533 3211 3485 3632 4043 2012
Size: 521 678 274 147 411 2792
Fragments arranged by size:
2792 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1193 1223 1364 1394 1193
Size: 30 141 30 4622
Fragments arranged by size:
4622 141 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 361 412 901 361
Size: 51 489 4283
Fragments arranged by size:
4283 489 51
BseYI C'CCAG_C
Cuts at: 2248 3006 3583 4079 2248
Size: 758 577 496 2992
Fragments arranged by size:
2992 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 2365 2411 3619 104
Size: 2261 46 1208 1308
Fragments arranged by size:
2261 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1529 1556 1670 1837 2615 2805 3597 1529
Size: 27 114 167 778 190 792 2755
Fragments arranged by size:
2755 792 778 190 167 114 27
BsiWI C'GTAC_G
Cuts at: 769 769
Size: 4823
BsmI GAATG_Cn'
Cuts at: 569 1353 569
Size: 784 4039
Bsp1286I G_dGCh'C
Cuts at: 250 833 1281 1529 1556 1670 1837 2448 2541
Size: 583 448 248 27 114 167 611 93
Cuts at: 2541 2615 2805 2867 3597 250
Size: 74 190 62 730 1476
Fragments arranged by size:
1476 730 611 583 448 248 190 167
114 93 74 62 27
BspEI T'CCGG_A
Cuts at: 4043 4043
Size: 4823
BspHI T'CATG_A
Cuts at: 3999 3999
Size: 4823
BspMI ACCTGCnnnn'nnnn_
Cuts at: 454 1706 1725 1873 2087 2389 2770 454
Size: 1252 19 148 214 302 381 2507
Fragments arranged by size:
2507 1252 381 302 214 148 19
BsrBI CCG'CTC
Cuts at: 50 3115 3169 4653 4818 50
Size: 3065 54 1484 165 55
Fragments arranged by size:
3065 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 707 945 2735 707
Size: 238 1790 2795
Fragments arranged by size:
2795 1790 238
BsrFI r'CCGG_y
Cuts at: 2821 3002 3211 2821
Size: 181 209 4433
Fragments arranged by size:
4433 209 181
BssSI C'ACGA_G
Cuts at: 3094 3452 3094
Size: 358 4465
BstAPI GCAn_nnn'nTGC
Cuts at: 245 1158 1348 2200 245
Size: 913 190 852 2868
Fragments arranged by size:
2868 913 852 190
BstBI TT'CG_AA
Cuts at: 3184 3184
Size: 4823
BstXI CCAn_nnnn'nTGG
Cuts at: 298 298
Size: 4823
BstYI r'GATC_y
Cuts at: 1 1080 1848 2315 2338 2673 2919 3931 4017
Size: 1079 768 467 23 335 246 1012 86
Cuts at: 4017 4029 4375 1
Size: 12 346 449
Fragments arranged by size:
1079 1012 768 467 449 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 1383 1383
Size: 4823
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 194 2773 2954 3117 194
Size: 2579 181 163 1900
Fragments arranged by size:
2579 1900 181 163
BtsI GCAGTG_nn'
Cuts at: 833 1782 2066 833
Size: 949 284 3590
Fragments arranged by size:
3590 949 284
DraI TTT'AAA
Cuts at: 1822 1822
Size: 4823
DrdI GACnn_nn'nnGTC
Cuts at: 2529 3387 2529
Size: 858 3965
EaeI y'GGCC_r
Cuts at: 1739 2408 2582 2973 3000 4345 1739
Size: 669 174 391 27 1345 2217
Fragments arranged by size:
2217 1345 669 391 174 27
EagI C'GGCC_G
Cuts at: 2408 2408
Size: 4823
EarI CTCTTCn'nnn_
Cuts at: 1645 2846 3056 1645
Size: 1201 210 3412
Fragments arranged by size:
3412 1201 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 352 3031 3341 3487 4633 352
Size: 2679 310 146 1146 542
Fragments arranged by size:
2679 1146 542 310 146
EcoICRI GAG'CTC
Cuts at: 1554 1835 1554
Size: 281 4542
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1513 1570 1669 2648 3080 3827 4095 1513
Size: 57 99 979 432 747 268 2241
Fragments arranged by size:
2241 979 747 432 268 99 57
EcoNI CCTnn'n_nnAGG
Cuts at: 1341 1341
Size: 4823
EcoO109I rG'GnC_Cy
Cuts at: 339 1788 1962 339
Size: 1449 174 3200
Fragments arranged by size:
3200 1449 174
EcoRI G'AATT_C
Cuts at: 1827 1827
Size: 4823
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2842 2842
Size: 4823
FspI TGC'GCA
Cuts at: 2604 2604
Size: 4823
HaeII r_GCGC'y
Cuts at: 2257 2505 3527 4506 2257
Size: 248 1022 979 2574
Fragments arranged by size:
2574 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 570 602 918 950 1193 1225 2665 2695 2697
Size: 32 316 32 243 32 1440 30 2
Cuts at: 2697 2727 570
Size: 30 2666
Fragments arranged by size:
2666 1440 316 243 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1862 1862
Size: 4823
KasI G'GCGC_C
Cuts at: 2253 2501 2253
Size: 248 4575
KpnI G_GTAC'C
Cuts at: 1843 1843
Size: 4823
MluI A'CGCG_T
Cuts at: 4256 4256
Size: 4823
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3494 3678 3494
Size: 184 4639
MscI TGG'CCA
Cuts at: 1741 2584 1741
Size: 843 3980
MslI CAynn'nnrTG
Cuts at: 117 1747 2812 2939 117
Size: 1630 1065 127 2001
Fragments arranged by size:
2001 1630 1065 127
MspA1I CmG'CkG
Cuts at: 298 515 1731 1934 2248 2608 3621 3866 298
Size: 217 1216 203 314 360 1013 245 1255
Fragments arranged by size:
1255 1216 1013 360 314 245 217 203
NaeI GCC'GGC
Cuts at: 3004 3004
Size: 4823
NarI GG'CG_CC
Cuts at: 2254 2502 2254
Size: 248 4575
NgoMIV G'CCGG_C
Cuts at: 3002 3002
Size: 4823
NspI r_CATG'y
Cuts at: 453 743 1876 2376 3283 453
Size: 290 1133 500 907 1993
Fragments arranged by size:
1993 1133 907 500 290
PciI A'CATG_T
Cuts at: 739 3279 739
Size: 2540 2283
PflMI CCAn_nnn'nTGG
Cuts at: 763 763
Size: 4823
PfoI T'CCnGG_A
Cuts at: 1547 1547
Size: 4823
PmeI GTTT'AAAC
Cuts at: 1822 1822
Size: 4823
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3962 3994 3962
Size: 32 4791
PpuMI rG'GwC_Cy
Cuts at: 339 339
Size: 4823
PsiI TTA'TAA
Cuts at: 653 653
Size: 4823
PstI C_TGCA'G
Cuts at: 1736 1870 2555 1736
Size: 134 685 4004
Fragments arranged by size:
4004 685 134
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4823
PvuII CAG'CTG
Cuts at: 298 515 1731 2248 2608 298
Size: 217 1216 517 360 2513
Fragments arranged by size:
2513 1216 517 360 217
RsrII CG'GwC_CG
Cuts at: 3018 3018
Size: 4823
SacI G_AGCT'C
Cuts at: 1556 1837 1556
Size: 281 4542
SalI G'TCGA_C
Cuts at: 1860 1860
Size: 4823
SapI GCTCTTCn'nnn_
Cuts at: 2846 3056 2846
Size: 210 4613
SbfI CC_TGCA'GG
Cuts at: 1870 1870
Size: 4823
ScaI AGT'ACT
Cuts at: 4340 4340
Size: 4823
SfcI C'TryA_G
Cuts at: 33 279 1732 1866 2551 3544 3735 33
Size: 246 1453 134 685 993 191 1121
Fragments arranged by size:
1453 1121 993 685 246 191 134
SfoI GGC'GCC
Cuts at: 2255 2503 2255
Size: 248 4575
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4823
SmaI CCC'GGG
Cuts at: 1845 1845
Size: 4823
SmlI C'TyrA_G
Cuts at: 343 696 1084 1555 1978 2893 3385 3647 343
Size: 353 388 471 423 915 492 262 1519
Fragments arranged by size:
1519 915 492 471 423 388 353 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4823
SphI G_CATG'C
Cuts at: 1876 2376 1876
Size: 500 4323
StyI C'CwwG_G
Cuts at: 491 1142 1957 2311 491
Size: 651 815 354 3003
Fragments arranged by size:
3003 815 651 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1445 2406 2539 2998 3207 1445
Size: 961 133 459 209 3061
Fragments arranged by size:
3061 961 459 209 133
TatI w'GTAC_w
Cuts at: 1608 1613 4338 1608
Size: 5 2725 2093
Fragments arranged by size:
2725 2093 5
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4340 4372 4340
Size: 32 4791
Tth111I GACn'n_nGTC
Cuts at: 2620 2620
Size: 4823
XbaI T'CTAG_A
Cuts at: 1854 1854
Size: 4823
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2318 2318
Size: 4823
XmaI C'CCGG_G
Cuts at: 1843 1843
Size: 4823
XmnI GAAnn'nnTTC
Cuts at: 500 500
Size: 4823
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BciVI BclI BglII BlpI Bme1580I
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI BsiWI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NgoMIV NspI PciI PflMI PfoI PmeI PpiI PpuMI
PsiI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AloI ApaI AscI AvrII
BaeI BbsI BbvCI BcgI BfrBI BglI BmgBI BmtI
Bpu10I BsgI BsmBI BsrGI BssHII BstEII BstZ17I Bsu36I
ClaI CspCI DraIII EcoRV FseI FspAI HindIII HpaI
MfeI NcoI NdeI NheI NotI NruI NsiI PacI
PasI PmlI PshAI PspOMI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI
XhoI ZraI