Restriction map for
pF1KB5985
(Circular) (Six-base) MAPSORT of: pF1KB5985.seq Check: 1712 from: 1 to: 4676
pF1KB5985 4676 bp
With 182 enzymes: SgfI *
June 26, 2008 10:45 ..
AccI GT'mk_AC
Cuts at: 1714 1714
Size: 4676
Acc65I G'GTAC_C
Cuts at: 1692 1692
Size: 4676
AclI AA'CG_TT
Cuts at: 4516 4516
Size: 4676
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1366 1604 2501 2933 3680 3948 1366
Size: 238 897 432 747 268 2094
Fragments arranged by size:
2094 897 747 432 268 238
AfeI AGC'GCT
Cuts at: 1318 1318
Size: 4676
AflIII A'CryG_T
Cuts at: 1330 2028 3132 4109 1330
Size: 698 1104 977 1897
Fragments arranged by size:
1897 1104 977 698
AgeI A'CCGG_T
Cuts at: 1490 3064 1490
Size: 1574 3102
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1597 1629 1597
Size: 32 4644
AlwNI CAG_nnn'CTG
Cuts at: 277 680 855 1981 3548 277
Size: 403 175 1126 1567 1405
Fragments arranged by size:
1567 1405 1126 403 175
ApaI G_GGCC'C
Cuts at: 1002 1185 1002
Size: 183 4493
ApaLI G'TGCA_C
Cuts at: 3446 3446
Size: 4676
ApoI r'AATT_y
Cuts at: 1680 4029 4285 1680
Size: 2349 256 2071
Fragments arranged by size:
2349 2071 256
AscI GG'CGCG_CC
Cuts at: 1477 1477
Size: 4676
AseI AT'TA_AT
Cuts at: 20 20
Size: 4676
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4676
AvaI C'yCGr_G
Cuts at: 126 142 1002 1171 1421 1696 126
Size: 16 860 169 250 275 3106
Fragments arranged by size:
3106 860 275 250 169 16
BamHI G'GATC_C
Cuts at: 1701 1701
Size: 4676
BanI G'GyrC_C
Cuts at: 469 1692 2106 2354 2389 469
Size: 1223 414 248 35 2756
Fragments arranged by size:
2756 1223 414 248 35
BanII G_rGCy'C
Cuts at: 151 364 1002 1121 1185 1690 2720 151
Size: 213 638 119 64 505 1030 2107
Fragments arranged by size:
2107 1030 638 505 213 119 64
BbeI G_GCGC'C
Cuts at: 2110 2358 2110
Size: 248 4428
BciVI GTATCCnnnnn_n'
Cuts at: 2574 3335 4676 2574
Size: 761 1341 2574
Fragments arranged by size:
2574 1341 761
BclI T'GATC_A
Cuts at: 2196 2196
Size: 4676
BglI GCCn_nnn'nGGC
Cuts at: 997 997
Size: 4676
BglII A'GATC_T
Cuts at: 2191 4228 2191
Size: 2037 2639
BlpI GC'TnA_GC
Cuts at: 506 1788 506
Size: 1282 3394
Bme1580I G_kGCm'C
Cuts at: 1002 1185 1422 2301 2394 3450 1002
Size: 183 237 879 93 1056 2228
Fragments arranged by size:
2228 1056 879 237 183 93
BmrI ACTGGGnnnn_n'
Cuts at: 668 1005 1888 1969 2304 4448 668
Size: 337 883 81 335 2144 896
Fragments arranged by size:
2144 896 883 337 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3043 3043
Size: 4676
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 231 702 1489 1624 231
Size: 471 787 135 3283
Fragments arranged by size:
3283 787 471 135
Bpu10I CC'TnA_GC
Cuts at: 1152 1189 1152
Size: 37 4639
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 239 1078 1237 1852 2731 3223 3521 239
Size: 839 159 615 879 492 298 1394
Fragments arranged by size:
1394 879 839 615 492 298 159
BsaI GGTCTCn'nnnn_
Cuts at: 274 424 4380 274
Size: 150 3956 570
Fragments arranged by size:
3956 570 150
BsaAI yAC'GTr
Cuts at: 1287 1331 2029 1287
Size: 44 698 3934
Fragments arranged by size:
3934 698 44
BsaBI GATnn'nnATC
Cuts at: 6 769 2190 4227 6
Size: 763 1421 2037 455
Fragments arranged by size:
2037 1421 763 455
BsaHI Gr'CG_yC
Cuts at: 984 1388 1433 2107 2355 3057 4553 984
Size: 404 45 674 248 702 1496 1107
Fragments arranged by size:
1496 1107 702 674 404 248 45
BsaWI w'CCGG_w
Cuts at: 236 881 1490 1865 2386 3064 3338 3485 3896
Size: 645 609 375 521 678 274 147 411
Cuts at: 3896 236
Size: 1016
Fragments arranged by size:
1016 678 645 609 521 411 375 274
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1597 1627 1597
Size: 30 4646
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 811 1096 1503 811
Size: 285 407 3984
Fragments arranged by size:
3984 407 285
BseYI C'CCAG_C
Cuts at: 306 422 449 545 583 834 1122 1638 2101
Size: 116 27 96 38 251 288 516 463
Cuts at: 2101 2859 3436 3932 306
Size: 758 577 496 1050
Fragments arranged by size:
1050 758 577 516 496 463 288 251
116 96 38 27
BsiEI CG_ry'CG
Cuts at: 104 1038 1274 2218 2264 3472 104
Size: 934 236 944 46 1208 1308
Fragments arranged by size:
1308 1208 944 934 236 46
BsiHKAI G_wGCw'C
Cuts at: 247 1121 1690 2468 2658 3450 247
Size: 874 569 778 190 792 1473
Fragments arranged by size:
1473 874 792 778 569 190
BsmBI CGTCTCn'nnnn_
Cuts at: 965 965
Size: 4676
Bsp1286I G_dGCh'C
Cuts at: 151 247 364 1002 1121 1185 1422 1690 2301
Size: 96 117 638 119 64 237 268 611
Cuts at: 2301 2394 2468 2658 2720 3450 151
Size: 93 74 190 62 730 1377
Fragments arranged by size:
1377 730 638 611 268 237 190 119
117 96 93 74 64 62
BspEI T'CCGG_A
Cuts at: 3896 3896
Size: 4676
BspHI T'CATG_A
Cuts at: 3852 3852
Size: 4676
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1726 1940 2242 2623 1726
Size: 214 302 381 3779
Fragments arranged by size:
3779 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1151 2968 3022 4506 4671 50
Size: 1101 1817 54 1484 165 55
Fragments arranged by size:
1817 1484 1101 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2588 2588
Size: 4676
BsrFI r'CCGG_y
Cuts at: 888 1108 1490 2674 2855 3064 888
Size: 220 382 1184 181 209 2500
Fragments arranged by size:
2500 1184 382 220 209 181
BssHII G'CGCG_C
Cuts at: 1477 1477
Size: 4676
BssSI C'ACGA_G
Cuts at: 1264 2947 3305 1264
Size: 1683 358 2635
Fragments arranged by size:
2635 1683 358
BstAPI GCAn_nnn'nTGC
Cuts at: 2053 2053
Size: 4676
BstBI TT'CG_AA
Cuts at: 3037 3037
Size: 4676
BstYI r'GATC_y
Cuts at: 1 273 686 1607 1701 2168 2191 2526 2772
Size: 272 413 921 94 467 23 335 246
Cuts at: 2772 3784 3870 3882 4228 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 921 467 449 413 346 335 272
246 94 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1079 1079
Size: 4676
BtgI C'CryG_G
Cuts at: 107 1427 107
Size: 1320 3356
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2626 2807 2970 2626
Size: 181 163 4332
Fragments arranged by size:
4332 181 163
BtsI GCAGTG_nn'
Cuts at: 302 1223 1919 302
Size: 921 696 3059
Fragments arranged by size:
3059 921 696
DraI TTT'AAA
Cuts at: 1675 1675
Size: 4676
DraIII CAC_nnn'GTG
Cuts at: 1266 1266
Size: 4676
DrdI GACnn_nn'nnGTC
Cuts at: 2382 3240 2382
Size: 858 3818
EaeI y'GGCC_r
Cuts at: 860 989 1099 1271 2261 2435 2826 2853 4198
Size: 129 110 172 990 174 391 27 1345
Cuts at: 4198 860
Size: 1338
Fragments arranged by size:
1345 1338 990 391 174 172 129 110
27
EagI C'GGCC_G
Cuts at: 1271 2261 1271
Size: 990 3686
EarI CTCTTCn'nnn_
Cuts at: 422 490 1459 2699 2909 422
Size: 68 969 1240 210 2189
Fragments arranged by size:
2189 1240 969 210 68
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 452 1023 2884 3194 3340 4486 452
Size: 571 1861 310 146 1146 642
Fragments arranged by size:
1861 1146 642 571 310 146
EcoICRI GAG'CTC
Cuts at: 1119 1688 1119
Size: 569 4107
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 231 702 1366 1489 1604 1624 2501 2933 3680
Size: 471 664 123 115 20 877 432 747
Cuts at: 3680 3948 231
Size: 268 959
Fragments arranged by size:
959 877 747 664 471 432 268 123
115 20
EcoNI CCTnn'n_nnAGG
Cuts at: 223 223
Size: 4676
EcoO109I rG'GnC_Cy
Cuts at: 123 214 332 840 999 1156 1181 1182 1569
Size: 91 118 508 159 157 25 1 387
Cuts at: 1569 1815 123
Size: 246 2984
Fragments arranged by size:
2984 508 387 246 159 157 118 91
25 1
EcoRI G'AATT_C
Cuts at: 1680 1680
Size: 4676
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1038 1263 2695 1038
Size: 225 1432 3019
Fragments arranged by size:
3019 1432 225
FspI TGC'GCA
Cuts at: 2457 2457
Size: 4676
HaeII r_GCGC'y
Cuts at: 166 1196 1320 2110 2358 3380 4359 166
Size: 1030 124 790 248 1022 979 483
Fragments arranged by size:
1030 1022 979 790 483 248 124
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2518 2548 2550 2580 2518
Size: 30 2 30 4614
Fragments arranged by size:
4614 30 30 2
HincII GTy'rAC
Cuts at: 315 1715 315
Size: 1400 3276
HpaI GTT'AAC
Cuts at: 315 315
Size: 4676
KasI G'GCGC_C
Cuts at: 2106 2354 2106
Size: 248 4428
KpnI G_GTAC'C
Cuts at: 1696 1696
Size: 4676
MluI A'CGCG_T
Cuts at: 4109 4109
Size: 4676
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 335 3347 3531 335
Size: 3012 184 1480
Fragments arranged by size:
3012 1480 184
MscI TGG'CCA
Cuts at: 862 1101 2437 862
Size: 239 1336 3101
Fragments arranged by size:
3101 1336 239
MslI CAynn'nnrTG
Cuts at: 964 1540 2665 2792 964
Size: 576 1125 127 2848
Fragments arranged by size:
2848 1125 576 127
MspA1I CmG'CkG
Cuts at: 306 583 1429 1445 1787 2101 2461 3474 3719
Size: 277 846 16 342 314 360 1013 245
Cuts at: 3719 306
Size: 1263
Fragments arranged by size:
1263 1013 846 360 342 314 277 245
16
NaeI GCC'GGC
Cuts at: 1110 2857 1110
Size: 1747 2929
NarI GG'CG_CC
Cuts at: 2107 2355 2107
Size: 248 4428
NcoI C'CATG_G
Cuts at: 107 107
Size: 4676
NgoMIV G'CCGG_C
Cuts at: 1108 2855 1108
Size: 1747 2929
NspI r_CATG'y
Cuts at: 903 1729 2229 3136 903
Size: 826 500 907 2443
Fragments arranged by size:
2443 907 826 500
PasI CC'CwG_GG
Cuts at: 154 597 1228 154
Size: 443 631 3602
Fragments arranged by size:
3602 631 443
PciI A'CATG_T
Cuts at: 3132 3132
Size: 4676
PflMI CCAn_nnn'nTGG
Cuts at: 680 855 680
Size: 175 4501
PfoI T'CCnGG_A
Cuts at: 1601 1601
Size: 4676
PmeI GTTT'AAAC
Cuts at: 1675 1675
Size: 4676
PmlI CAC'GTG
Cuts at: 1287 1331 1287
Size: 44 4632
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1597 1629 3815 3847 1597
Size: 32 2186 32 2426
Fragments arranged by size:
2426 2186 32 32
PpuMI rG'GwC_Cy
Cuts at: 214 332 840 214
Size: 118 508 4050
Fragments arranged by size:
4050 508 118
PspOMI G'GGCC_C
Cuts at: 998 1181 998
Size: 183 4493
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 1642 1674 1642
Size: 32 4644
PstI C_TGCA'G
Cuts at: 917 1723 2408 917
Size: 806 685 3185
Fragments arranged by size:
3185 806 685
PvuI CG_AT'CG
Cuts at: 104 1038 104
Size: 934 3742
PvuII CAG'CTG
Cuts at: 306 583 2101 2461 306
Size: 277 1518 360 2521
Fragments arranged by size:
2521 1518 360 277
RsrII CG'GwC_CG
Cuts at: 234 2871 234
Size: 2637 2039
SacI G_AGCT'C
Cuts at: 1121 1690 1121
Size: 569 4107
SacII CC_GC'GG
Cuts at: 1430 1430
Size: 4676
SalI G'TCGA_C
Cuts at: 1713 1713
Size: 4676
SanDI GG'GwC_CC
Cuts at: 214 332 840 214
Size: 118 508 4050
Fragments arranged by size:
4050 508 118
SapI GCTCTTCn'nnn_
Cuts at: 2699 2909 2699
Size: 210 4466
SbfI CC_TGCA'GG
Cuts at: 1723 1723
Size: 4676
ScaI AGT'ACT
Cuts at: 4193 4193
Size: 4676
SfcI C'TryA_G
Cuts at: 33 913 1551 1719 2404 3397 3588 33
Size: 880 638 168 685 993 191 1121
Fragments arranged by size:
1121 993 880 685 638 191 168
SfiI GGCCn_nnn'nGGCC
Cuts at: 997 997
Size: 4676
SfoI GGC'GCC
Cuts at: 2108 2356 2108
Size: 248 4428
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4676
SmaI CCC'GGG
Cuts at: 128 1004 1698 128
Size: 876 694 3106
Fragments arranged by size:
3106 876 694
SmlI C'TyrA_G
Cuts at: 142 254 1093 1252 1831 2746 3238 3500 142
Size: 112 839 159 579 915 492 262 1318
Fragments arranged by size:
1318 915 839 579 492 262 159 112
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4676
SphI G_CATG'C
Cuts at: 1729 2229 1729
Size: 500 4176
StyI C'CwwG_G
Cuts at: 107 809 1056 1810 2164 107
Size: 702 247 754 354 2619
Fragments arranged by size:
2619 754 702 354 247
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 422 1614 2259 2392 2851 3060 422
Size: 1192 645 133 459 209 2038
Fragments arranged by size:
2038 1192 645 459 209 133
TatI w'GTAC_w
Cuts at: 1649 4191 1649
Size: 2542 2134
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 226 258 4193 4225 226
Size: 32 3935 32 677
Fragments arranged by size:
3935 677 32 32
Tth111I GACn'n_nGTC
Cuts at: 1537 2473 1537
Size: 936 3740
XbaI T'CTAG_A
Cuts at: 1707 1707
Size: 4676
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 114 255 1203 2171 114
Size: 141 948 968 2619
Fragments arranged by size:
2619 968 948 141
XhoI C'TCGA_G
Cuts at: 142 142
Size: 4676
XmaI C'CCGG_G
Cuts at: 126 1002 1696 126
Size: 876 694 3106
Fragments arranged by size:
3106 876 694
XmnI GAAnn'nnTTC
Cuts at: 1021 1561 1021
Size: 540 4136
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AloI
AlwNI ApaI ApaLI ApoI AscI AseI AsiSI AvaI
BamHI BanI BanII BbeI BciVI BclI BglI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI
BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssHII BssSI BstAPI BstBI BstYI Bsu36I BtgI
BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HpaI KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PspOMI PsrI PstI PvuI PvuII RsrII SacI SacII
SalI SanDI SapI SbfI ScaI SfcI SfiI SfoI
SgfI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AflII AhdI AleI AlfI AvrII BaeI
BbsI BbvCI BcgI BfrBI BmgBI BmtI BsgI BsiWI
BsmI BsrGI BstEII BstXI BstZ17I ClaI CspCI EcoRV
FseI FspAI HindIII MfeI NdeI NheI NotI NruI
NsiI PacI PshAI PsiI PspXI SexAI SgrAI SnaBI
SrfI SspI StuI SwaI ZraI