Restriction map for
pF1KB5978
(Circular) (Six-base) MAPSORT of: pF1KB5978.seq Check: 4250 from: 1 to: 4805
pF1KB5978 4805 bp
With 182 enzymes: SgfI *
October 7, 2009 14:19 ..
AccI GT'mk_AC
Cuts at: 1843 1843
Size: 4805
Acc65I G'GTAC_C
Cuts at: 1172 1821 1172
Size: 649 4156
AclI AA'CG_TT
Cuts at: 4645 4645
Size: 4805
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 189 937 1462 1671 2630 3062 3809 4077 189
Size: 748 525 209 959 432 747 268 917
Fragments arranged by size:
959 917 748 747 525 432 268 209
AflIII A'CryG_T
Cuts at: 2157 3261 4238 2157
Size: 1104 977 2724
Fragments arranged by size:
2724 1104 977
AgeI A'CCGG_T
Cuts at: 3193 3193
Size: 4805
AleI CACnn'nnGTG
Cuts at: 1057 1057
Size: 4805
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1265 1265
Size: 4805
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 730 762 730
Size: 32 4773
AlwNI CAG_nnn'CTG
Cuts at: 735 840 1278 1428 1458 2110 3677 735
Size: 105 438 150 30 652 1567 1863
Fragments arranged by size:
1863 1567 652 438 150 105 30
ApaI G_GGCC'C
Cuts at: 481 481
Size: 4805
ApaLI G'TGCA_C
Cuts at: 3575 3575
Size: 4805
ApoI r'AATT_y
Cuts at: 904 1809 4158 4414 904
Size: 905 2349 256 1295
Fragments arranged by size:
2349 1295 905 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4805
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4805
AvaI C'yCGr_G
Cuts at: 1825 1825
Size: 4805
BamHI G'GATC_C
Cuts at: 1830 1830
Size: 4805
BanI G'GyrC_C
Cuts at: 391 712 1172 1603 1821 2235 2483 2518 391
Size: 321 460 431 218 414 248 35 2678
Fragments arranged by size:
2678 460 431 414 321 248 218 35
BanII G_rGCy'C
Cuts at: 307 481 824 1819 2849 307
Size: 174 343 995 1030 2263
Fragments arranged by size:
2263 1030 995 343 174
BbeI G_GCGC'C
Cuts at: 395 2239 2487 395
Size: 1844 248 2713
Fragments arranged by size:
2713 1844 248
BbvCI CC'TCA_GC
Cuts at: 590 1112 1157 1723 590
Size: 522 45 566 3672
Fragments arranged by size:
3672 566 522 45
BciVI GTATCCnnnnn_n'
Cuts at: 2703 3464 4805 2703
Size: 761 1341 2703
Fragments arranged by size:
2703 1341 761
BclI T'GATC_A
Cuts at: 2325 2325
Size: 4805
BglI GCCn_nnn'nGGC
Cuts at: 200 302 200
Size: 102 4703
BglII A'GATC_T
Cuts at: 1282 2320 4357 1282
Size: 1038 2037 1730
Fragments arranged by size:
2037 1730 1038
BlpI GC'TnA_GC
Cuts at: 1343 1917 1343
Size: 574 4231
Bme1580I G_kGCm'C
Cuts at: 481 499 715 2430 2523 3579 481
Size: 18 216 1715 93 1056 1707
Fragments arranged by size:
1715 1707 1056 216 93 18
BmgBI CAC'GTC
Cuts at: 1299 1485 1299
Size: 186 4619
BmrI ACTGGGnnnn_n'
Cuts at: 2017 2098 2433 4577 2017
Size: 81 335 2144 2245
Fragments arranged by size:
2245 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3172 3172
Size: 4805
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 213 524 679 757 1417 1534 1572 1761 213
Size: 311 155 78 660 117 38 189 3257
Fragments arranged by size:
3257 660 311 189 155 117 78 38
Bpu10I CC'TnA_GC
Cuts at: 590 1112 1157 1626 1723 590
Size: 522 45 469 97 3672
Fragments arranged by size:
3672 522 469 97 45
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1351 1981 2860 3352 3650 1351
Size: 630 879 492 298 2506
Fragments arranged by size:
2506 879 630 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4509 4509
Size: 4805
BsaAI yAC'GTr
Cuts at: 2158 2158
Size: 4805
BsaBI GATnn'nnATC
Cuts at: 6 2319 4356 6
Size: 2313 2037 455
Fragments arranged by size:
2313 2037 455
BsaHI Gr'CG_yC
Cuts at: 392 2236 2484 3186 4682 392
Size: 1844 248 702 1496 515
Fragments arranged by size:
1844 1496 702 515 248
BsaWI w'CCGG_w
Cuts at: 1994 2515 3193 3467 3614 4025 1994
Size: 521 678 274 147 411 2774
Fragments arranged by size:
2774 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 730 760 730
Size: 30 4775
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 278 607 1147 1204 1537 1761 1764 278
Size: 329 540 57 333 224 3 3319
Fragments arranged by size:
3319 540 333 329 224 57 3
BseYI C'CCAG_C
Cuts at: 2230 2988 3565 4061 2230
Size: 758 577 496 2974
Fragments arranged by size:
2974 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1548 1594 1548
Size: 46 4759
BsiEI CG_ry'CG
Cuts at: 104 2347 2393 3601 104
Size: 2243 46 1208 1308
Fragments arranged by size:
2243 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 824 1819 2597 2787 3579 824
Size: 995 778 190 792 2050
Fragments arranged by size:
2050 995 792 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 219 1590 219
Size: 1371 3434
Bsp1286I G_dGCh'C
Cuts at: 307 481 499 715 824 1819 2430 2523 2597
Size: 174 18 216 109 995 611 93 74
Cuts at: 2597 2787 2849 3579 307
Size: 190 62 730 1533
Fragments arranged by size:
1533 995 730 611 216 190 174 109
93 74 62 18
BspEI T'CCGG_A
Cuts at: 4025 4025
Size: 4805
BspHI T'CATG_A
Cuts at: 3981 3981
Size: 4805
BspMI ACCTGCnnnn'nnnn_
Cuts at: 563 1188 1235 1328 1855 2069 2371 2752 563
Size: 625 47 93 527 214 302 381 2616
Fragments arranged by size:
2616 625 527 381 302 214 93 47
BsrBI CCG'CTC
Cuts at: 50 197 227 3097 3151 4635 4800 50
Size: 147 30 2870 54 1484 165 55
Fragments arranged by size:
2870 1484 165 147 55 54 30
BsrDI GCAATG_nn'
Cuts at: 1331 2717 1331
Size: 1386 3419
BsrFI r'CCGG_y
Cuts at: 424 454 2803 2984 3193 424
Size: 30 2349 181 209 2036
Fragments arranged by size:
2349 2036 209 181 30
BssSI C'ACGA_G
Cuts at: 3076 3434 3076
Size: 358 4447
BstAPI GCAn_nnn'nTGC
Cuts at: 2182 2182
Size: 4805
BstBI TT'CG_AA
Cuts at: 3166 3166
Size: 4805
BstEII G'GTnAC_C
Cuts at: 519 519
Size: 4805
BstYI r'GATC_y
Cuts at: 1 314 1282 1830 2297 2320 2655 2901 3913
Size: 313 968 548 467 23 335 246 1012
Cuts at: 3913 3999 4011 4357 1
Size: 86 12 346 449
Fragments arranged by size:
1012 968 548 467 449 346 335 313
246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 769 769
Size: 4805
BtgI C'CryG_G
Cuts at: 107 1070 107
Size: 963 3842
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1092 2755 2936 3099 1092
Size: 1663 181 163 2798
Fragments arranged by size:
2798 1663 181 163
BtsI GCAGTG_nn'
Cuts at: 243 1315 1626 1668 1704 2048 243
Size: 1072 311 42 36 344 3000
Fragments arranged by size:
3000 1072 344 311 42 36
ClaI AT'CG_AT
Cuts at: 563 563
Size: 4805
DraI TTT'AAA
Cuts at: 1804 1804
Size: 4805
DrdI GACnn_nn'nnGTC
Cuts at: 790 2511 3369 790
Size: 1721 858 2226
Fragments arranged by size:
2226 1721 858
EaeI y'GGCC_r
Cuts at: 110 2390 2564 2955 2982 4327 110
Size: 2280 174 391 27 1345 588
Fragments arranged by size:
2280 1345 588 391 174 27
EagI C'GGCC_G
Cuts at: 2390 2390
Size: 4805
EarI CTCTTCn'nnn_
Cuts at: 1780 2828 3038 1780
Size: 1048 210 3547
Fragments arranged by size:
3547 1048 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3013 3323 3469 4615 3013
Size: 310 146 1146 3203
Fragments arranged by size:
3203 1146 310 146
EcoICRI GAG'CTC
Cuts at: 822 1817 822
Size: 995 3810
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 189 213 524 679 757 937 1417 1462 1534
Size: 24 311 155 78 180 480 45 72
Cuts at: 1534 1572 1671 1761 2630 3062 3809 4077 189
Size: 38 99 90 869 432 747 268 917
Fragments arranged by size:
917 869 747 480 432 311 268 180
155 99 90 78 72 45 38 24
EcoNI CCTnn'n_nnAGG
Cuts at: 700 844 1411 1779 700
Size: 144 567 368 3726
Fragments arranged by size:
3726 567 368 144
EcoO109I rG'GnC_Cy
Cuts at: 477 478 837 1425 1944 477
Size: 1 359 588 519 3338
Fragments arranged by size:
3338 588 519 359 1
EcoRI G'AATT_C
Cuts at: 1809 1809
Size: 4805
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2824 2824
Size: 4805
FspI TGC'GCA
Cuts at: 1231 2586 1231
Size: 1355 3450
HaeII r_GCGC'y
Cuts at: 395 2239 2487 3509 4488 395
Size: 1844 248 1022 979 712
Fragments arranged by size:
1844 1022 979 712 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1150 1182 1274 1306 2647 2677 2679 2709 1150
Size: 32 92 32 1341 30 2 30 3246
Fragments arranged by size:
3246 1341 92 32 32 30 30 2
HincII GTy'rAC
Cuts at: 528 1560 1844 528
Size: 1032 284 3489
Fragments arranged by size:
3489 1032 284
KasI G'GCGC_C
Cuts at: 391 2235 2483 391
Size: 1844 248 2713
Fragments arranged by size:
2713 1844 248
KpnI G_GTAC'C
Cuts at: 1176 1825 1176
Size: 649 4156
MluI A'CGCG_T
Cuts at: 4238 4238
Size: 4805
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1530 1656 1713 3476 3660 1530
Size: 126 57 1763 184 2675
Fragments arranged by size:
2675 1763 184 126 57
MscI TGG'CCA
Cuts at: 112 2566 112
Size: 2454 2351
MslI CAynn'nnrTG
Cuts at: 1057 1567 2794 2921 1057
Size: 510 1227 127 2941
Fragments arranged by size:
2941 1227 510 127
MspA1I CmG'CkG
Cuts at: 335 579 780 1916 2230 2590 3603 3848 335
Size: 244 201 1136 314 360 1013 245 1292
Fragments arranged by size:
1292 1136 1013 360 314 245 244 201
NaeI GCC'GGC
Cuts at: 426 456 2986 426
Size: 30 2530 2245
Fragments arranged by size:
2530 2245 30
NarI GG'CG_CC
Cuts at: 392 2236 2484 392
Size: 1844 248 2713
Fragments arranged by size:
2713 1844 248
NcoI C'CATG_G
Cuts at: 107 1070 107
Size: 963 3842
NgoMIV G'CCGG_C
Cuts at: 424 454 2984 424
Size: 30 2530 2245
Fragments arranged by size:
2530 2245 30
NspI r_CATG'y
Cuts at: 260 834 1858 2358 3265 260
Size: 574 1024 500 907 1800
Fragments arranged by size:
1800 1024 907 574 500
PasI CC'CwG_GG
Cuts at: 184 184
Size: 4805
PciI A'CATG_T
Cuts at: 3261 3261
Size: 4805
PfoI T'CCnGG_A
Cuts at: 656 656
Size: 4805
PmeI GTTT'AAAC
Cuts at: 1804 1804
Size: 4805
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 730 762 3944 3976 730
Size: 32 3182 32 1559
Fragments arranged by size:
3182 1559 32 32
PpuMI rG'GwC_Cy
Cuts at: 837 1425 837
Size: 588 4217
PspOMI G'GGCC_C
Cuts at: 477 477
Size: 4805
PstI C_TGCA'G
Cuts at: 704 1199 1325 1412 1852 2537 704
Size: 495 126 87 440 685 2972
Fragments arranged by size:
2972 685 495 440 126 87
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4805
PvuII CAG'CTG
Cuts at: 335 780 2230 2590 335
Size: 445 1450 360 2550
Fragments arranged by size:
2550 1450 445 360
RsrII CG'GwC_CG
Cuts at: 3000 3000
Size: 4805
SacI G_AGCT'C
Cuts at: 824 1819 824
Size: 995 3810
SalI G'TCGA_C
Cuts at: 1842 1842
Size: 4805
SapI GCTCTTCn'nnn_
Cuts at: 1780 2828 3038 1780
Size: 1048 210 3547
Fragments arranged by size:
3547 1048 210
SbfI CC_TGCA'GG
Cuts at: 1325 1852 1325
Size: 527 4278
ScaI AGT'ACT
Cuts at: 4322 4322
Size: 4805
SexAI A'CCwGG_T
Cuts at: 839 839
Size: 4805
SfcI C'TryA_G
Cuts at: 33 700 1195 1321 1408 1848 2533 3526 3717
Size: 667 495 126 87 440 685 993 191
Cuts at: 3717 33
Size: 1121
Fragments arranged by size:
1121 993 685 667 495 440 191 126
87
SfoI GGC'GCC
Cuts at: 393 2237 2485 393
Size: 1844 248 2713
Fragments arranged by size:
2713 1844 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4805
SmaI CCC'GGG
Cuts at: 1827 1827
Size: 4805
SmlI C'TyrA_G
Cuts at: 1366 1960 2875 3367 3629 1366
Size: 594 915 492 262 2542
Fragments arranged by size:
2542 915 594 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4805
SphI G_CATG'C
Cuts at: 834 1858 2358 834
Size: 1024 500 3281
Fragments arranged by size:
3281 1024 500
StuI AGG'CCT
Cuts at: 1631 1631
Size: 4805
StyI C'CwwG_G
Cuts at: 107 965 1070 1939 2293 107
Size: 858 105 869 354 2619
Fragments arranged by size:
2619 869 858 354 105
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 238 433 1624 2388 2521 2980 3189 238
Size: 195 1191 764 133 459 209 1854
Fragments arranged by size:
1854 1191 764 459 209 195 133
TatI w'GTAC_w
Cuts at: 755 1757 4320 755
Size: 1002 2563 1240
Fragments arranged by size:
2563 1240 1002
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 673 705 4322 4354 673
Size: 32 3617 32 1124
Fragments arranged by size:
3617 1124 32 32
Tth111I GACn'n_nGTC
Cuts at: 1297 2602 1297
Size: 1305 3500
XbaI T'CTAG_A
Cuts at: 1836 1836
Size: 4805
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1383 1425 2300 1383
Size: 42 875 3888
Fragments arranged by size:
3888 875 42
XmaI C'CCGG_G
Cuts at: 1825 1825
Size: 4805
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlfI
AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbvCI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII
BstYI Bsu36I BtgI BtgZI BtsI ClaI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PasI PciI PfoI PmeI PpiI PpuMI
PspOMI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AscI AvrII BaeI
BbsI BcgI BfrBI BmtI BsiWI BsmI BsrGI BssHII
BstXI BstZ17I CspCI DraIII EcoRV FseI FspAI HindIII
HpaI MfeI NdeI NheI NotI NruI NsiI PacI
PflMI PmlI PshAI PsiI PspXI PsrI SacII SanDI
SfiI SgrAI SnaBI SrfI SspI SwaI XhoI XmnI
ZraI