Restriction map for
pF1KB5971
(Circular) (Six-base) MAPSORT of: pF1KB5971.seq Check: 8887 from: 1 to: 4667
pF1KB5971 4667 bp
With 182 enzymes: SgfI *
June 26, 2008 10:45 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 892 892
Size: 4667
AccI GT'mk_AC
Cuts at: 1171 1705 1171
Size: 534 4133
Acc65I G'GTAC_C
Cuts at: 1683 1683
Size: 4667
AclI AA'CG_TT
Cuts at: 4507 4507
Size: 4667
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 306 413 1306 1418 2492 2924 3671 3939 306
Size: 107 893 112 1074 432 747 268 1034
Fragments arranged by size:
1074 1034 893 747 432 268 112 107
AflIII A'CryG_T
Cuts at: 569 1586 2019 3123 4100 569
Size: 1017 433 1104 977 1136
Fragments arranged by size:
1136 1104 1017 977 433
AgeI A'CCGG_T
Cuts at: 3055 3055
Size: 4667
AleI CACnn'nnGTG
Cuts at: 1077 1077
Size: 4667
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1083 1083
Size: 4667
AlwNI CAG_nnn'CTG
Cuts at: 185 388 901 1972 3539 185
Size: 203 513 1071 1567 1313
Fragments arranged by size:
1567 1313 1071 513 203
ApaI G_GGCC'C
Cuts at: 507 833 1496 507
Size: 326 663 3678
Fragments arranged by size:
3678 663 326
ApaLI G'TGCA_C
Cuts at: 1080 3437 1080
Size: 2357 2310
ApoI r'AATT_y
Cuts at: 1671 4020 4276 1671
Size: 2349 256 2062
Fragments arranged by size:
2349 2062 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4667
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4667
AvaI C'yCGr_G
Cuts at: 1196 1601 1687 1196
Size: 405 86 4176
Fragments arranged by size:
4176 405 86
BamHI G'GATC_C
Cuts at: 1692 1692
Size: 4667
BanI G'GyrC_C
Cuts at: 806 904 1108 1683 2097 2345 2380 806
Size: 98 204 575 414 248 35 3093
Fragments arranged by size:
3093 575 414 248 204 98 35
BanII G_rGCy'C
Cuts at: 284 486 507 833 1012 1496 1681 2711 284
Size: 202 21 326 179 484 185 1030 2240
Fragments arranged by size:
2240 1030 484 326 202 185 179 21
BbeI G_GCGC'C
Cuts at: 908 2101 2349 908
Size: 1193 248 3226
Fragments arranged by size:
3226 1193 248
BbsI GAAGACnn'nnnn_
Cuts at: 556 1387 556
Size: 831 3836
BbvCI CC'TCA_GC
Cuts at: 1244 1244
Size: 4667
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 208 242 208
Size: 34 4633
BciVI GTATCCnnnnn_n'
Cuts at: 2565 3326 4667 2565
Size: 761 1341 2565
Fragments arranged by size:
2565 1341 761
BclI T'GATC_A
Cuts at: 683 2187 683
Size: 1504 3163
BglI GCCn_nnn'nGGC
Cuts at: 196 407 606 664 196
Size: 211 199 58 4199
Fragments arranged by size:
4199 211 199 58
BglII A'GATC_T
Cuts at: 2182 4219 2182
Size: 2037 2630
BlpI GC'TnA_GC
Cuts at: 1779 1779
Size: 4667
Bme1580I G_kGCm'C
Cuts at: 507 833 1072 1084 1496 2292 2385 3441 507
Size: 326 239 12 412 796 93 1056 1733
Fragments arranged by size:
1733 1056 796 412 326 239 93 12
BmrI ACTGGGnnnn_n'
Cuts at: 376 738 1244 1879 1960 2295 4439 376
Size: 362 506 635 81 335 2144 604
Fragments arranged by size:
2144 635 604 506 362 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3034 3034
Size: 4667
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 229 1370 1668 229
Size: 1141 298 3228
Fragments arranged by size:
3228 1141 298
Bpu10I CC'TnA_GC
Cuts at: 602 1244 602
Size: 642 4025
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1059 1102 1843 2722 3214 3512 1059
Size: 43 741 879 492 298 2214
Fragments arranged by size:
2214 879 741 492 298 43
BsaI GGTCTCn'nnnn_
Cuts at: 141 4371 141
Size: 4230 437
BsaAI yAC'GTr
Cuts at: 2020 2020
Size: 4667
BsaBI GATnn'nnATC
Cuts at: 6 2181 4218 6
Size: 2175 2037 455
Fragments arranged by size:
2175 2037 455
BsaHI Gr'CG_yC
Cuts at: 905 2098 2346 3048 4544 905
Size: 1193 248 702 1496 1028
Fragments arranged by size:
1496 1193 1028 702 248
BsaWI w'CCGG_w
Cuts at: 1856 2377 3055 3329 3476 3887 1856
Size: 521 678 274 147 411 2636
Fragments arranged by size:
2636 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 910 940 1609 1639 910
Size: 30 669 30 3938
Fragments arranged by size:
3938 669 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 499 1614 499
Size: 1115 3552
BseYI C'CCAG_C
Cuts at: 178 945 1112 1657 2092 2850 3427 3923 178
Size: 767 167 545 435 758 577 496 922
Fragments arranged by size:
922 767 758 577 545 496 435 167
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1052 1052
Size: 4667
BsiEI CG_ry'CG
Cuts at: 104 2209 2255 3463 104
Size: 2105 46 1208 1308
Fragments arranged by size:
2105 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1084 1681 2459 2649 3441 1084
Size: 597 778 190 792 2310
Fragments arranged by size:
2310 792 778 597 190
BsmI GAATG_Cn'
Cuts at: 754 754
Size: 4667
Bsp1286I G_dGCh'C
Cuts at: 284 486 507 833 1012 1072 1084 1496 1681
Size: 202 21 326 179 60 12 412 185
Cuts at: 1681 2292 2385 2459 2649 2711 3441 284
Size: 611 93 74 190 62 730 1510
Fragments arranged by size:
1510 730 611 412 326 202 190 185
179 93 74 62 60 21 12
BspEI T'CCGG_A
Cuts at: 3887 3887
Size: 4667
BspHI T'CATG_A
Cuts at: 1370 3843 1370
Size: 2473 2194
BspMI ACCTGCnnnn'nnnn_
Cuts at: 892 1717 1931 2233 2614 892
Size: 825 214 302 381 2945
Fragments arranged by size:
2945 825 381 302 214
BsrBI CCG'CTC
Cuts at: 50 606 2959 3013 4497 4662 50
Size: 556 2353 54 1484 165 55
Fragments arranged by size:
2353 1484 556 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2579 2579
Size: 4667
BsrFI r'CCGG_y
Cuts at: 2665 2846 3055 2665
Size: 181 209 4277
Fragments arranged by size:
4277 209 181
BssSI C'ACGA_G
Cuts at: 2938 3296 2938
Size: 358 4309
BstAPI GCAn_nnn'nTGC
Cuts at: 2044 2044
Size: 4667
BstBI TT'CG_AA
Cuts at: 3028 3028
Size: 4667
BstEII G'GTnAC_C
Cuts at: 694 694
Size: 4667
BstYI r'GATC_y
Cuts at: 1 524 1121 1692 2159 2182 2517 2763 3775
Size: 523 597 571 467 23 335 246 1012
Cuts at: 3775 3861 3873 4219 1
Size: 86 12 346 449
Fragments arranged by size:
1012 597 571 523 467 449 346 335
246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 998 998
Size: 4667
BtgI C'CryG_G
Cuts at: 194 200 194
Size: 6 4661
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2617 2798 2961 2617
Size: 181 163 4323
Fragments arranged by size:
4323 181 163
BtsI GCAGTG_nn'
Cuts at: 1017 1406 1910 1017
Size: 389 504 3774
Fragments arranged by size:
3774 504 389
ClaI AT'CG_AT
Cuts at: 839 839
Size: 4667
DraI TTT'AAA
Cuts at: 1666 1666
Size: 4667
DrdI GACnn_nn'nnGTC
Cuts at: 2373 3231 2373
Size: 858 3809
EaeI y'GGCC_r
Cuts at: 197 1403 2252 2426 2817 2844 4189 197
Size: 1206 849 174 391 27 1345 675
Fragments arranged by size:
1345 1206 849 675 391 174 27
EagI C'GGCC_G
Cuts at: 2252 2252
Size: 4667
EarI CTCTTCn'nnn_
Cuts at: 752 1282 1385 2690 2900 752
Size: 530 103 1305 210 2519
Fragments arranged by size:
2519 1305 530 210 103
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2875 3185 3331 4477 2875
Size: 310 146 1146 3065
Fragments arranged by size:
3065 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1679 1679
Size: 4667
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 229 306 413 1306 1370 1418 1668 2492 2924
Size: 77 107 893 64 48 250 824 432
Cuts at: 2924 3671 3939 229
Size: 747 268 957
Fragments arranged by size:
957 893 824 747 432 268 250 107
77 64 48
EcoO109I rG'GnC_Cy
Cuts at: 503 504 762 829 830 875 956 1492 1493
Size: 1 258 67 1 45 81 536 1
Cuts at: 1493 1561 1598 1606 1806 503
Size: 68 37 8 200 3364
Fragments arranged by size:
3364 536 258 200 81 68 67 45
37 8 1 1 1
EcoRI G'AATT_C
Cuts at: 1671 1671
Size: 4667
EcoRV GAT'ATC
Cuts at: 234 234
Size: 4667
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 642 1311 2686 642
Size: 669 1375 2623
Fragments arranged by size:
2623 1375 669
FspI TGC'GCA
Cuts at: 1501 2448 1501
Size: 947 3720
HaeII r_GCGC'y
Cuts at: 908 1367 2101 2349 3371 4350 908
Size: 459 734 248 1022 979 1225
Fragments arranged by size:
1225 1022 979 734 459 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1609 1641 2509 2539 2541 2571 1609
Size: 32 868 30 2 30 3705
Fragments arranged by size:
3705 868 32 30 30 2
HincII GTy'rAC
Cuts at: 741 1172 1706 741
Size: 431 534 3702
Fragments arranged by size:
3702 534 431
KasI G'GCGC_C
Cuts at: 904 2097 2345 904
Size: 1193 248 3226
Fragments arranged by size:
3226 1193 248
KpnI G_GTAC'C
Cuts at: 1687 1687
Size: 4667
MluI A'CGCG_T
Cuts at: 4100 4100
Size: 4667
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 882 1142 3338 3522 882
Size: 260 2196 184 2027
Fragments arranged by size:
2196 2027 260 184
MscI TGG'CCA
Cuts at: 1405 2428 1405
Size: 1023 3644
MslI CAynn'nnrTG
Cuts at: 731 1077 2656 2783 731
Size: 346 1579 127 2615
Fragments arranged by size:
2615 1579 346 127
MspA1I CmG'CkG
Cuts at: 851 1778 2092 2452 3465 3710 851
Size: 927 314 360 1013 245 1808
Fragments arranged by size:
1808 1013 927 360 314 245
NaeI GCC'GGC
Cuts at: 2848 2848
Size: 4667
NarI GG'CG_CC
Cuts at: 905 2098 2346 905
Size: 1193 248 3226
Fragments arranged by size:
3226 1193 248
NgoMIV G'CCGG_C
Cuts at: 2846 2846
Size: 4667
NspI r_CATG'y
Cuts at: 1211 1590 1720 2220 3127 1211
Size: 379 130 500 907 2751
Fragments arranged by size:
2751 907 500 379 130
PasI CC'CwG_GG
Cuts at: 592 766 592
Size: 174 4493
PciI A'CATG_T
Cuts at: 1586 3123 1586
Size: 1537 3130
PfoI T'CCnGG_A
Cuts at: 245 245
Size: 4667
PmeI GTTT'AAAC
Cuts at: 1666 1666
Size: 4667
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3806 3838 3806
Size: 32 4635
PpuMI rG'GwC_Cy
Cuts at: 762 956 1561 1606 762
Size: 194 605 45 3823
Fragments arranged by size:
3823 605 194 45
PshAI GACnn'nnGTC
Cuts at: 961 961
Size: 4667
PsiI TTA'TAA
Cuts at: 305 305
Size: 4667
PspOMI G'GGCC_C
Cuts at: 503 829 1492 503
Size: 326 663 3678
Fragments arranged by size:
3678 663 326
PstI C_TGCA'G
Cuts at: 1181 1714 2399 1181
Size: 533 685 3449
Fragments arranged by size:
3449 685 533
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4667
PvuII CAG'CTG
Cuts at: 2092 2452 2092
Size: 360 4307
RsrII CG'GwC_CG
Cuts at: 2862 2862
Size: 4667
SacI G_AGCT'C
Cuts at: 1681 1681
Size: 4667
SalI G'TCGA_C
Cuts at: 1170 1704 1170
Size: 534 4133
SapI GCTCTTCn'nnn_
Cuts at: 1282 2690 2900 1282
Size: 1408 210 3049
Fragments arranged by size:
3049 1408 210
SbfI CC_TGCA'GG
Cuts at: 1714 1714
Size: 4667
ScaI AGT'ACT
Cuts at: 4184 4184
Size: 4667
SfcI C'TryA_G
Cuts at: 33 1177 1710 2395 3388 3579 33
Size: 1144 533 685 993 191 1121
Fragments arranged by size:
1144 1121 993 685 533 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 407 407
Size: 4667
SfoI GGC'GCC
Cuts at: 906 2099 2347 906
Size: 1193 248 3226
Fragments arranged by size:
3226 1193 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4667
SmaI CCC'GGG
Cuts at: 1198 1603 1689 1198
Size: 405 86 4176
Fragments arranged by size:
4176 405 86
SmlI C'TyrA_G
Cuts at: 1038 1117 1822 2737 3229 3491 1038
Size: 79 705 915 492 262 2214
Fragments arranged by size:
2214 915 705 492 262 79
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4667
SphI G_CATG'C
Cuts at: 1720 2220 1720
Size: 500 4167
StuI AGG'CCT
Cuts at: 401 600 982 1242 401
Size: 199 382 260 3826
Fragments arranged by size:
3826 382 260 199
StyI C'CwwG_G
Cuts at: 1801 2155 1801
Size: 354 4313
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 436 682 1018 1126 2250 2383 2842 3051 436
Size: 246 336 108 1124 133 459 209 2052
Fragments arranged by size:
2052 1124 459 336 246 209 133 108
TatI w'GTAC_w
Cuts at: 572 4182 572
Size: 3610 1057
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4184 4216 4184
Size: 32 4635
Tth111I GACn'n_nGTC
Cuts at: 2464 2464
Size: 4667
XbaI T'CTAG_A
Cuts at: 1698 1698
Size: 4667
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 114 589 773 919 2162 114
Size: 475 184 146 1243 2619
Fragments arranged by size:
2619 1243 475 184 146
XmaI C'CCGG_G
Cuts at: 1196 1601 1687 1196
Size: 405 86 4176
Fragments arranged by size:
4176 405 86
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NgoMIV NspI PasI PciI PfoI PmeI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfiI SfoI SgfI
SmaI SmlI SpeI SphI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AloI AscI AvrII BaeI
BfrBI BmgBI BmtI BsiWI BsmBI BsrGI BssHII BstXI
BstZ17I CspCI DraIII EcoNI FseI FspAI HindIII HpaI
MfeI NcoI NdeI NheI NotI NruI NsiI PacI
PflMI PmlI PspXI PsrI SacII SanDI SexAI SgrAI
SnaBI SrfI SspI SwaI XhoI XmnI ZraI