Restriction map for
pF1KB5956
(Circular) (Six-base) MAPSORT of: pF1KB5956.seq Check: 4076 from: 1 to: 4757
pF1KB5956 4757 bp
With 182 enzymes: SgfI *
June 26, 2008 10:45 ..
AccI GT'mk_AC
Cuts at: 1795 1795
Size: 4757
Acc65I G'GTAC_C
Cuts at: 1773 1773
Size: 4757
AclI AA'CG_TT
Cuts at: 4597 4597
Size: 4757
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2582 3014 3761 4029 2582
Size: 432 747 268 3310
Fragments arranged by size:
3310 747 432 268
AflIII A'CryG_T
Cuts at: 2109 3213 4190 2109
Size: 1104 977 2676
Fragments arranged by size:
2676 1104 977
AgeI A'CCGG_T
Cuts at: 3145 3145
Size: 4757
AleI CACnn'nnGTG
Cuts at: 861 861
Size: 4757
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 694 694
Size: 4757
AlwNI CAG_nnn'CTG
Cuts at: 558 2062 3629 558
Size: 1504 1567 1686
Fragments arranged by size:
1686 1567 1504
ApaI G_GGCC'C
Cuts at: 163 1203 163
Size: 1040 3717
ApaLI G'TGCA_C
Cuts at: 3527 3527
Size: 4757
ApoI r'AATT_y
Cuts at: 1761 4110 4366 1761
Size: 2349 256 2152
Fragments arranged by size:
2349 2152 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4757
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4757
AvaI C'yCGr_G
Cuts at: 872 945 1777 872
Size: 73 832 3852
Fragments arranged by size:
3852 832 73
BamHI G'GATC_C
Cuts at: 1782 1782
Size: 4757
BanI G'GyrC_C
Cuts at: 130 1108 1773 2187 2435 2470 130
Size: 978 665 414 248 35 2417
Fragments arranged by size:
2417 978 665 414 248 35
BanII G_rGCy'C
Cuts at: 163 1203 1748 1771 2801 163
Size: 1040 545 23 1030 2119
Fragments arranged by size:
2119 1040 1030 545 23
BbeI G_GCGC'C
Cuts at: 2191 2439 2191
Size: 248 4509
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 264 298 264
Size: 34 4723
BciVI GTATCCnnnnn_n'
Cuts at: 2655 3416 4757 2655
Size: 761 1341 2655
Fragments arranged by size:
2655 1341 761
BclI T'GATC_A
Cuts at: 701 2277 701
Size: 1576 3181
BglI GCCn_nnn'nGGC
Cuts at: 593 1135 593
Size: 542 4215
BglII A'GATC_T
Cuts at: 2272 4309 2272
Size: 2037 2720
BlpI GC'TnA_GC
Cuts at: 1869 1869
Size: 4757
Bme1580I G_kGCm'C
Cuts at: 135 163 466 746 1111 1203 2382 2475 3531
Size: 28 303 280 365 92 1179 93 1056
Cuts at: 3531 135
Size: 1361
Fragments arranged by size:
1361 1179 1056 365 303 280 93 92
28
BmrI ACTGGGnnnn_n'
Cuts at: 116 159 1197 1969 2050 2385 4529 116
Size: 43 1038 772 81 335 2144 344
Fragments arranged by size:
2144 1038 772 344 335 81 43
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3124 3124
Size: 4757
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 459 664 910 459
Size: 205 246 4306
Fragments arranged by size:
4306 246 205
Bpu10I CC'TnA_GC
Cuts at: 1272 1272
Size: 4757
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 364 1933 2812 3304 3602 364
Size: 1569 879 492 298 1519
Fragments arranged by size:
1569 1519 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1230 1651 4461 1230
Size: 421 2810 1526
Fragments arranged by size:
2810 1526 421
BsaAI yAC'GTr
Cuts at: 1365 2110 1365
Size: 745 4012
BsaBI GATnn'nnATC
Cuts at: 6 541 2271 4308 6
Size: 535 1730 2037 455
Fragments arranged by size:
2037 1730 535 455
BsaHI Gr'CG_yC
Cuts at: 2188 2436 3138 4634 2188
Size: 248 702 1496 2311
Fragments arranged by size:
2311 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1946 2467 3145 3419 3566 3977 1946
Size: 521 678 274 147 411 2726
Fragments arranged by size:
2726 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 883 913 883
Size: 30 4727
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 379 1414 1474 1609 379
Size: 1035 60 135 3527
Fragments arranged by size:
3527 1035 135 60
BseYI C'CCAG_C
Cuts at: 135 2182 2940 3517 4013 135
Size: 2047 758 577 496 879
Fragments arranged by size:
2047 879 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1501 1636 1723 1501
Size: 135 87 4535
Fragments arranged by size:
4535 135 87
BsiEI CG_ry'CG
Cuts at: 104 470 545 2299 2345 3553 104
Size: 366 75 1754 46 1208 1308
Fragments arranged by size:
1754 1308 1208 366 75 46
BsiHKAI G_wGCw'C
Cuts at: 1771 2549 2739 3531 1771
Size: 778 190 792 2997
Fragments arranged by size:
2997 792 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 477 656 477
Size: 179 4578
Bsp1286I G_dGCh'C
Cuts at: 135 163 466 746 1111 1203 1748 1771 2382
Size: 28 303 280 365 92 545 23 611
Cuts at: 2382 2475 2549 2739 2801 3531 135
Size: 93 74 190 62 730 1361
Fragments arranged by size:
1361 730 611 545 365 303 280 190
93 92 74 62 28 23
BspEI T'CCGG_A
Cuts at: 3977 3977
Size: 4757
BspHI T'CATG_A
Cuts at: 3933 3933
Size: 4757
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1733 1807 2021 2323 2704 1733
Size: 74 214 302 381 3786
Fragments arranged by size:
3786 381 302 214 74
BsrBI CCG'CTC
Cuts at: 50 3049 3103 4587 4752 50
Size: 2999 54 1484 165 55
Fragments arranged by size:
2999 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1533 2669 1533
Size: 1136 3621
BsrFI r'CCGG_y
Cuts at: 2755 2936 3145 2755
Size: 181 209 4367
Fragments arranged by size:
4367 209 181
BssSI C'ACGA_G
Cuts at: 3028 3386 3028
Size: 358 4399
BstAPI GCAn_nnn'nTGC
Cuts at: 2134 2134
Size: 4757
BstBI TT'CG_AA
Cuts at: 3118 3118
Size: 4757
BstYI r'GATC_y
Cuts at: 1 1782 2249 2272 2607 2853 3865 3951 3963
Size: 1781 467 23 335 246 1012 86 12
Cuts at: 3963 4309 1
Size: 346 449
Fragments arranged by size:
1781 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 155 1475 1544 107
Size: 48 1320 69 3320
Fragments arranged by size:
3320 1320 69 48
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2707 2888 3051 2707
Size: 181 163 4413
Fragments arranged by size:
4413 181 163
BtsI GCAGTG_nn'
Cuts at: 2000 2000
Size: 4757
DraI TTT'AAA
Cuts at: 1756 1756
Size: 4757
DrdI GACnn_nn'nnGTC
Cuts at: 571 852 1222 2463 3321 571
Size: 281 370 1241 858 2007
Fragments arranged by size:
2007 1241 858 370 281
EaeI y'GGCC_r
Cuts at: 201 1127 1403 1664 2342 2516 2907 2934 4279
Size: 926 276 261 678 174 391 27 1345
Cuts at: 4279 201
Size: 679
Fragments arranged by size:
1345 926 679 678 391 276 261 174
27
EagI C'GGCC_G
Cuts at: 2342 2342
Size: 4757
EarI CTCTTCn'nnn_
Cuts at: 2780 2990 2780
Size: 210 4547
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1696 2965 3275 3421 4567 1696
Size: 1269 310 146 1146 1886
Fragments arranged by size:
1886 1269 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1769 1769
Size: 4757
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 459 664 910 2582 3014 3761 4029 459
Size: 205 246 1672 432 747 268 1187
Fragments arranged by size:
1672 1187 747 432 268 246 205
EcoO109I rG'GnC_Cy
Cuts at: 160 615 1199 1896 160
Size: 455 584 697 3021
Fragments arranged by size:
3021 697 584 455
EcoRI G'AATT_C
Cuts at: 1761 1761
Size: 4757
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 636 2776 636
Size: 2140 2617
FspI TGC'GCA
Cuts at: 2538 2538
Size: 4757
HaeII r_GCGC'y
Cuts at: 2191 2439 3461 4440 2191
Size: 248 1022 979 2508
Fragments arranged by size:
2508 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 214 246 866 898 1066 1098 2599 2629 2631
Size: 32 620 32 168 32 1501 30 2
Cuts at: 2631 2661 214
Size: 30 2310
Fragments arranged by size:
2310 1501 620 168 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1359 1796 1359
Size: 437 4320
KasI G'GCGC_C
Cuts at: 2187 2435 2187
Size: 248 4509
KpnI G_GTAC'C
Cuts at: 1777 1777
Size: 4757
MluI A'CGCG_T
Cuts at: 4190 4190
Size: 4757
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 263 587 1017 1082 3428 3612 263
Size: 324 430 65 2346 184 1408
Fragments arranged by size:
2346 1408 430 324 184 65
MscI TGG'CCA
Cuts at: 203 1666 2518 203
Size: 1463 852 2442
Fragments arranged by size:
2442 1463 852
MslI CAynn'nnrTG
Cuts at: 861 1513 2746 2873 861
Size: 652 1233 127 2745
Fragments arranged by size:
2745 1233 652 127
MspA1I CmG'CkG
Cuts at: 142 888 1398 1868 2182 2542 3555 3800 142
Size: 746 510 470 314 360 1013 245 1099
Fragments arranged by size:
1099 1013 746 510 470 360 314 245
NaeI GCC'GGC
Cuts at: 2938 2938
Size: 4757
NarI GG'CG_CC
Cuts at: 2188 2436 2188
Size: 248 4509
NcoI C'CATG_G
Cuts at: 107 155 1544 107
Size: 48 1389 3320
Fragments arranged by size:
3320 1389 48
NgoMIV G'CCGG_C
Cuts at: 2936 2936
Size: 4757
NspI r_CATG'y
Cuts at: 1474 1575 1810 2310 3217 1474
Size: 101 235 500 907 3014
Fragments arranged by size:
3014 907 500 235 101
PciI A'CATG_T
Cuts at: 3213 3213
Size: 4757
PfoI T'CCnGG_A
Cuts at: 1553 1553
Size: 4757
PmeI GTTT'AAAC
Cuts at: 1756 1756
Size: 4757
PmlI CAC'GTG
Cuts at: 1365 1365
Size: 4757
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3896 3928 3896
Size: 32 4725
PpuMI rG'GwC_Cy
Cuts at: 615 615
Size: 4757
PsiI TTA'TAA
Cuts at: 653 653
Size: 4757
PspOMI G'GGCC_C
Cuts at: 159 1199 159
Size: 1040 3717
PstI C_TGCA'G
Cuts at: 457 1804 2489 457
Size: 1347 685 2725
Fragments arranged by size:
2725 1347 685
PvuI CG_AT'CG
Cuts at: 104 470 545 104
Size: 366 75 4316
Fragments arranged by size:
4316 366 75
PvuII CAG'CTG
Cuts at: 2182 2542 2182
Size: 360 4397
RsrII CG'GwC_CG
Cuts at: 2952 2952
Size: 4757
SacI G_AGCT'C
Cuts at: 1771 1771
Size: 4757
SalI G'TCGA_C
Cuts at: 1794 1794
Size: 4757
SapI GCTCTTCn'nnn_
Cuts at: 2780 2990 2780
Size: 210 4547
SbfI CC_TGCA'GG
Cuts at: 1804 1804
Size: 4757
ScaI AGT'ACT
Cuts at: 1414 4274 1414
Size: 2860 1897
SfcI C'TryA_G
Cuts at: 33 453 1800 2485 3478 3669 33
Size: 420 1347 685 993 191 1121
Fragments arranged by size:
1347 1121 993 685 420 191
SfoI GGC'GCC
Cuts at: 2189 2437 2189
Size: 248 4509
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4757
SmaI CCC'GGG
Cuts at: 1779 1779
Size: 4757
SmlI C'TyrA_G
Cuts at: 379 872 1912 2827 3319 3581 379
Size: 493 1040 915 492 262 1555
Fragments arranged by size:
1555 1040 915 493 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4757
SphI G_CATG'C
Cuts at: 1474 1575 1810 2310 1474
Size: 101 235 500 3921
Fragments arranged by size:
3921 500 235 101
StuI AGG'CCT
Cuts at: 495 495
Size: 4757
StyI C'CwwG_G
Cuts at: 107 155 746 1544 1661 1891 2245 107
Size: 48 591 798 117 230 354 2619
Fragments arranged by size:
2619 798 591 354 230 117 48
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2340 2473 2932 3141 2340
Size: 133 459 209 3956
Fragments arranged by size:
3956 459 209 133
TatI w'GTAC_w
Cuts at: 707 1412 4272 707
Size: 705 2860 1192
Fragments arranged by size:
2860 1192 705
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 709 741 883 915 4274 4306 709
Size: 32 142 32 3359 32 1160
Fragments arranged by size:
3359 1160 142 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 880 1441 2554 880
Size: 561 1113 3083
Fragments arranged by size:
3083 1113 561
XbaI T'CTAG_A
Cuts at: 1788 1788
Size: 4757
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 121 417 1512 1551 1674 2252 121
Size: 296 1095 39 123 578 2626
Fragments arranged by size:
2626 1095 578 296 123 39
XhoI C'TCGA_G
Cuts at: 872 872
Size: 4757
XmaI C'CCGG_G
Cuts at: 1777 1777
Size: 4757
XmnI GAAnn'nnTTC
Cuts at: 733 733
Size: 4757
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlfI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PciI PfoI PmeI PmlI
PpiI PpuMI PsiI PspOMI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AloI AscI AvrII
BaeI BbsI BbvCI BfrBI BmgBI BmtI BsiWI BsmI
BsrGI BssHII BstEII BstXI BstZ17I Bsu36I ClaI CspCI
DraIII EcoNI EcoRV FseI FspAI HindIII HpaI MfeI
NdeI NheI NotI NruI NsiI PacI PasI PflMI
PshAI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI SspI SwaI ZraI