Restriction map for
pF1KB5946
(Circular) (Six-base) MAPSORT of: pF1KB5946.seq Check: 8128 from: 1 to: 4598
pF1KB5946 4598 bp
With 182 enzymes: SgfI *
July 16, 2019 14:21 ..
AccI GT'mk_AC
Cuts at: 1636 1636
Size: 4598
Acc65I G'GTAC_C
Cuts at: 254 1614 254
Size: 1360 3238
AclI AA'CG_TT
Cuts at: 4438 4438
Size: 4598
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 523 1050 1087 1529 2423 2855 3602 3870 523
Size: 527 37 442 894 432 747 268 1251
Fragments arranged by size:
1251 894 747 527 442 432 268 37
AflII C'TTAA_G
Cuts at: 541 1075 541
Size: 534 4064
AflIII A'CryG_T
Cuts at: 331 1950 3054 4031 331
Size: 1619 1104 977 898
Fragments arranged by size:
1619 1104 977 898
AgeI A'CCGG_T
Cuts at: 2986 2986
Size: 4598
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 730 730
Size: 4598
AlwNI CAG_nnn'CTG
Cuts at: 127 1903 3470 127
Size: 1776 1567 1255
Fragments arranged by size:
1776 1567 1255
ApaI G_GGCC'C
Cuts at: 501 501
Size: 4598
ApaLI G'TGCA_C
Cuts at: 3368 3368
Size: 4598
ApoI r'AATT_y
Cuts at: 1324 1453 1602 3951 4207 1324
Size: 129 149 2349 256 1715
Fragments arranged by size:
2349 1715 256 149 129
AseI AT'TA_AT
Cuts at: 20 20
Size: 4598
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4598
AvaI C'yCGr_G
Cuts at: 811 1618 811
Size: 807 3791
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 318 351 318
Size: 33 4565
BamHI G'GATC_C
Cuts at: 1171 1623 1171
Size: 452 4146
BanI G'GyrC_C
Cuts at: 254 1614 2028 2276 2311 254
Size: 1360 414 248 35 2541
Fragments arranged by size:
2541 1360 414 248 35
BanII G_rGCy'C
Cuts at: 269 501 859 1124 1612 2642 269
Size: 232 358 265 488 1030 2225
Fragments arranged by size:
2225 1030 488 358 265 232
BbeI G_GCGC'C
Cuts at: 2032 2280 2032
Size: 248 4350
BbsI GAAGACnn'nnnn_
Cuts at: 104 104
Size: 4598
BciVI GTATCCnnnnn_n'
Cuts at: 2496 3257 4598 2496
Size: 761 1341 2496
Fragments arranged by size:
2496 1341 761
BclI T'GATC_A
Cuts at: 1299 2118 1299
Size: 819 3779
BglII A'GATC_T
Cuts at: 210 2113 4150 210
Size: 1903 2037 658
Fragments arranged by size:
2037 1903 658
BlpI GC'TnA_GC
Cuts at: 985 1710 985
Size: 725 3873
Bme1580I G_kGCm'C
Cuts at: 501 1550 2223 2316 3372 501
Size: 1049 673 93 1056 1727
Fragments arranged by size:
1727 1056 1049 673 93
BmrI ACTGGGnnnn_n'
Cuts at: 137 1199 1810 1891 2226 4370 137
Size: 1062 611 81 335 2144 365
Fragments arranged by size:
2144 1062 611 365 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1255 2965 1255
Size: 1710 2888
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 845 1134 1276 845
Size: 289 142 4167
Fragments arranged by size:
4167 289 142
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 902 1774 2653 3145 3443 902
Size: 872 879 492 298 2057
Fragments arranged by size:
2057 879 872 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4302 4302
Size: 4598
BsaAI yAC'GTr
Cuts at: 1951 1951
Size: 4598
BsaBI GATnn'nnATC
Cuts at: 6 209 2112 4149 6
Size: 203 1903 2037 455
Fragments arranged by size:
2037 1903 455 203
BsaHI Gr'CG_yC
Cuts at: 2029 2277 2979 4475 2029
Size: 248 702 1496 2152
Fragments arranged by size:
2152 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1787 2308 2986 3260 3407 3818 1787
Size: 521 678 274 147 411 2567
Fragments arranged by size:
2567 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 230 260 610 640 843 873 230
Size: 30 350 30 203 30 3955
Fragments arranged by size:
3955 350 203 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 259 259
Size: 4598
BseYI C'CCAG_C
Cuts at: 2023 2781 3358 3854 2023
Size: 758 577 496 2767
Fragments arranged by size:
2767 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 447 2140 2186 3394 104
Size: 343 1693 46 1208 1308
Fragments arranged by size:
1693 1308 1208 343 46
BsiHKAI G_wGCw'C
Cuts at: 859 991 1124 1337 1612 2390 2580 3372 859
Size: 132 133 213 275 778 190 792 2085
Fragments arranged by size:
2085 792 778 275 213 190 133 132
BsmI GAATG_Cn'
Cuts at: 455 455
Size: 4598
Bsp1286I G_dGCh'C
Cuts at: 269 501 859 991 1124 1337 1550 1612 2223
Size: 232 358 132 133 213 213 62 611
Cuts at: 2223 2316 2390 2580 2642 3372 269
Size: 93 74 190 62 730 1495
Fragments arranged by size:
1495 730 611 358 232 213 213 190
133 132 93 74 62 62
BspEI T'CCGG_A
Cuts at: 3818 3818
Size: 4598
BspHI T'CATG_A
Cuts at: 1250 3774 1250
Size: 2524 2074
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1648 1862 2164 2545 1648
Size: 214 302 381 3701
Fragments arranged by size:
3701 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2890 2944 4428 4593 50
Size: 2840 54 1484 165 55
Fragments arranged by size:
2840 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2510 2510
Size: 4598
BsrFI r'CCGG_y
Cuts at: 2596 2777 2986 2596
Size: 181 209 4208
Fragments arranged by size:
4208 209 181
BsrGI T'GTAC_A
Cuts at: 482 482
Size: 4598
BssSI C'ACGA_G
Cuts at: 2869 3227 2869
Size: 358 4240
BstAPI GCAn_nnn'nTGC
Cuts at: 1975 1975
Size: 4598
BstBI TT'CG_AA
Cuts at: 2959 2959
Size: 4598
BstEII G'GTnAC_C
Cuts at: 739 739
Size: 4598
BstXI CCAn_nnnn'nTGG
Cuts at: 751 1182 751
Size: 431 4167
BstYI r'GATC_y
Cuts at: 1 210 1171 1623 2090 2113 2448 2694 3706
Size: 209 961 452 467 23 335 246 1012
Cuts at: 3706 3792 3804 4150 1
Size: 86 12 346 449
Fragments arranged by size:
1012 961 467 452 449 346 335 246
209 86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2548 2729 2892 2548
Size: 181 163 4254
Fragments arranged by size:
4254 181 163
BtsI GCAGTG_nn'
Cuts at: 1841 1841
Size: 4598
DraI TTT'AAA
Cuts at: 149 183 1597 149
Size: 34 1414 3150
Fragments arranged by size:
3150 1414 34
DraIII CAC_nnn'GTG
Cuts at: 1427 1427
Size: 4598
DrdI GACnn_nn'nnGTC
Cuts at: 2304 3162 2304
Size: 858 3740
EaeI y'GGCC_r
Cuts at: 753 2183 2357 2748 2775 4120 753
Size: 1430 174 391 27 1345 1231
Fragments arranged by size:
1430 1345 1231 391 174 27
EagI C'GGCC_G
Cuts at: 2183 2183
Size: 4598
EarI CTCTTCn'nnn_
Cuts at: 212 1033 2621 2831 212
Size: 821 1588 210 1979
Fragments arranged by size:
1979 1588 821 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2806 3116 3262 4408 2806
Size: 310 146 1146 2996
Fragments arranged by size:
2996 1146 310 146
EcoICRI GAG'CTC
Cuts at: 857 1122 1610 857
Size: 265 488 3845
Fragments arranged by size:
3845 488 265
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 523 845 1050 1087 1134 1276 1529 2423 2855
Size: 322 205 37 47 142 253 894 432
Cuts at: 2855 3602 3870 523
Size: 747 268 1251
Fragments arranged by size:
1251 894 747 432 322 268 253 205
142 47 37
EcoO109I rG'GnC_Cy
Cuts at: 497 498 1737 497
Size: 1 1239 3358
Fragments arranged by size:
3358 1239 1
EcoRI G'AATT_C
Cuts at: 1602 1602
Size: 4598
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2617 2617
Size: 4598
FspI TGC'GCA
Cuts at: 2379 2379
Size: 4598
HaeII r_GCGC'y
Cuts at: 2032 2280 3302 4281 2032
Size: 248 1022 979 2349
Fragments arranged by size:
2349 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 232 264 843 875 2440 2470 2472 2502 232
Size: 32 579 32 1565 30 2 30 2328
Fragments arranged by size:
2328 1565 579 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1637 1637
Size: 4598
KasI G'GCGC_C
Cuts at: 2028 2276 2028
Size: 248 4350
KpnI G_GTAC'C
Cuts at: 258 1618 258
Size: 1360 3238
MluI A'CGCG_T
Cuts at: 4031 4031
Size: 4598
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 396 3269 3453 396
Size: 2873 184 1541
Fragments arranged by size:
2873 1541 184
MscI TGG'CCA
Cuts at: 755 2359 755
Size: 1604 2994
MslI CAynn'nnrTG
Cuts at: 706 712 1498 1535 1570 2587 2714 706
Size: 6 786 37 35 1017 127 2590
Fragments arranged by size:
2590 1017 786 127 37 35 6
MspA1I CmG'CkG
Cuts at: 1374 1709 2023 2383 3396 3641 1374
Size: 335 314 360 1013 245 2331
Fragments arranged by size:
2331 1013 360 335 314 245
NaeI GCC'GGC
Cuts at: 2779 2779
Size: 4598
NarI GG'CG_CC
Cuts at: 2029 2277 2029
Size: 248 4350
NdeI CA'TA_TG
Cuts at: 750 750
Size: 4598
NgoMIV G'CCGG_C
Cuts at: 2777 2777
Size: 4598
NspI r_CATG'y
Cuts at: 335 1582 1651 2151 3058 335
Size: 1247 69 500 907 1875
Fragments arranged by size:
1875 1247 907 500 69
PciI A'CATG_T
Cuts at: 331 3054 331
Size: 2723 1875
PfoI T'CCnGG_A
Cuts at: 1388 1388
Size: 4598
PmeI GTTT'AAAC
Cuts at: 1597 1597
Size: 4598
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3737 3769 3737
Size: 32 4566
PshAI GACnn'nnGTC
Cuts at: 680 680
Size: 4598
PspOMI G'GGCC_C
Cuts at: 497 497
Size: 4598
PstI C_TGCA'G
Cuts at: 1645 2330 1645
Size: 685 3913
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4598
PvuII CAG'CTG
Cuts at: 1374 2023 2383 1374
Size: 649 360 3589
Fragments arranged by size:
3589 649 360
RsrII CG'GwC_CG
Cuts at: 2793 2793
Size: 4598
SacI G_AGCT'C
Cuts at: 859 1124 1612 859
Size: 265 488 3845
Fragments arranged by size:
3845 488 265
SalI G'TCGA_C
Cuts at: 1635 1635
Size: 4598
SapI GCTCTTCn'nnn_
Cuts at: 212 2621 2831 212
Size: 2409 210 1979
Fragments arranged by size:
2409 1979 210
SbfI CC_TGCA'GG
Cuts at: 1645 1645
Size: 4598
ScaI AGT'ACT
Cuts at: 4115 4115
Size: 4598
SfcI C'TryA_G
Cuts at: 33 665 1641 2326 3319 3510 33
Size: 632 976 685 993 191 1121
Fragments arranged by size:
1121 993 976 685 632 191
SfoI GGC'GCC
Cuts at: 2030 2278 2030
Size: 248 4350
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4598
SmaI CCC'GGG
Cuts at: 1620 1620
Size: 4598
SmlI C'TyrA_G
Cuts at: 541 917 1075 1753 2668 3160 3422 541
Size: 376 158 678 915 492 262 1717
Fragments arranged by size:
1717 915 678 492 376 262 158
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4598
SphI G_CATG'C
Cuts at: 1582 1651 2151 1582
Size: 69 500 4029
Fragments arranged by size:
4029 500 69
StuI AGG'CCT
Cuts at: 635 635
Size: 4598
StyI C'CwwG_G
Cuts at: 1732 2086 1732
Size: 354 4244
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 461 2181 2314 2773 2982 461
Size: 1720 133 459 209 2077
Fragments arranged by size:
2077 1720 459 209 133
TatI w'GTAC_w
Cuts at: 482 4113 482
Size: 3631 967
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 230 262 610 642 4115 4147 230
Size: 32 348 32 3473 32 681
Fragments arranged by size:
3473 681 348 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2395 2395
Size: 4598
XbaI T'CTAG_A
Cuts at: 1629 1629
Size: 4598
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2093 2093
Size: 4598
XmaI C'CCGG_G
Cuts at: 1618 1618
Size: 4598
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AlfI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI
BamHI BanI BanII BbeI BbsI BciVI BclI BglII
BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssSI BstAPI BstBI BstEII BstXI BstYI BtgZI
BtsI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NdeI NgoMIV NspI PciI PfoI PmeI
PpiI PshAI PspOMI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AhdI AleI AloI AscI AvrII
BbvCI BcgI BfrBI BglI BmgBI BmtI Bpu10I BsgI
BsiWI BsmBI BssHII BstZ17I Bsu36I BtgI ClaI CspCI
EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NcoI
NheI NotI NruI NsiI PacI PasI PflMI PmlI
PpuMI PsiI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI SspI SwaI XhoI XmnI ZraI