Restriction map for
pF1KB5943
(Circular) (Six-base) MAPSORT of: pF1KB5943.seq Check: 2900 from: 1 to: 4637
pF1KB5943 4637 bp
With 182 enzymes: SgfI *
September 25, 2012 15:36 ..
AccI GT'mk_AC
Cuts at: 1675 1675
Size: 4637
Acc65I G'GTAC_C
Cuts at: 1653 1653
Size: 4637
AclI AA'CG_TT
Cuts at: 4477 4477
Size: 4637
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 833 907 2462 2894 3641 3909 833
Size: 74 1555 432 747 268 1561
Fragments arranged by size:
1561 1555 747 432 268 74
AflIII A'CryG_T
Cuts at: 1989 3093 4070 1989
Size: 1104 977 2556
Fragments arranged by size:
2556 1104 977
AgeI A'CCGG_T
Cuts at: 453 3025 453
Size: 2572 2065
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 182 182
Size: 4637
AlwNI CAG_nnn'CTG
Cuts at: 694 1942 3509 694
Size: 1248 1567 1822
Fragments arranged by size:
1822 1567 1248
ApaLI G'TGCA_C
Cuts at: 3407 3407
Size: 4637
ApoI r'AATT_y
Cuts at: 199 746 958 1580 1641 3990 4246 199
Size: 547 212 622 61 2349 256 590
Fragments arranged by size:
2349 622 590 547 256 212 61
AseI AT'TA_AT
Cuts at: 20 20
Size: 4637
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4637
AvaI C'yCGr_G
Cuts at: 345 1657 345
Size: 1312 3325
BamHI G'GATC_C
Cuts at: 111 1662 111
Size: 1551 3086
BanI G'GyrC_C
Cuts at: 166 1653 2067 2315 2350 166
Size: 1487 414 248 35 2453
Fragments arranged by size:
2453 1487 414 248 35
BanII G_rGCy'C
Cuts at: 139 308 410 1651 2681 139
Size: 169 102 1241 1030 2095
Fragments arranged by size:
2095 1241 1030 169 102
BbeI G_GCGC'C
Cuts at: 2071 2319 2071
Size: 248 4389
BbsI GAAGACnn'nnnn_
Cuts at: 210 535 668 895 1004 210
Size: 325 133 227 109 3843
Fragments arranged by size:
3843 325 227 133 109
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1414 1448 1414
Size: 34 4603
BciVI GTATCCnnnnn_n'
Cuts at: 2535 3296 4637 2535
Size: 761 1341 2535
Fragments arranged by size:
2535 1341 761
BclI T'GATC_A
Cuts at: 2157 2157
Size: 4637
BfrBI ATG'CAT
Cuts at: 437 437
Size: 4637
BglI GCCn_nnn'nGGC
Cuts at: 175 175
Size: 4637
BglII A'GATC_T
Cuts at: 554 2152 4189 554
Size: 1598 2037 1002
Fragments arranged by size:
2037 1598 1002
BlpI GC'TnA_GC
Cuts at: 1749 1749
Size: 4637
Bme1580I G_kGCm'C
Cuts at: 1420 2262 2355 3411 1420
Size: 842 93 1056 2646
Fragments arranged by size:
2646 1056 842 93
BmrI ACTGGGnnnn_n'
Cuts at: 555 1849 1930 2265 4409 555
Size: 1294 81 335 2144 783
Fragments arranged by size:
2144 1294 783 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 277 360 3004 277
Size: 83 2644 1910
Fragments arranged by size:
2644 1910 83
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 382 382
Size: 4637
Bpu10I CC'TnA_GC
Cuts at: 1267 1267
Size: 4637
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 153 173 1813 2692 3184 3482 153
Size: 20 1640 879 492 298 1308
Fragments arranged by size:
1640 1308 879 492 298 20
BsaI GGTCTCn'nnnn_
Cuts at: 144 1469 4341 144
Size: 1325 2872 440
Fragments arranged by size:
2872 1325 440
BsaAI yAC'GTr
Cuts at: 1990 1990
Size: 4637
BsaBI GATnn'nnATC
Cuts at: 6 846 2151 4188 6
Size: 840 1305 2037 455
Fragments arranged by size:
2037 1305 840 455
BsaHI Gr'CG_yC
Cuts at: 2068 2316 3018 4514 2068
Size: 248 702 1496 2191
Fragments arranged by size:
2191 1496 702 248
BsaWI w'CCGG_w
Cuts at: 453 1077 1109 1826 2347 3025 3299 3446 3857
Size: 624 32 717 521 678 274 147 411
Cuts at: 3857 453
Size: 1233
Fragments arranged by size:
1233 717 678 624 521 411 274 147
32
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 392 422 392
Size: 30 4607
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 171 176 400 799 171
Size: 5 224 399 4009
Fragments arranged by size:
4009 399 224 5
BseYI C'CCAG_C
Cuts at: 2062 2820 3397 3893 2062
Size: 758 577 496 2806
Fragments arranged by size:
2806 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 2179 2225 3433 104
Size: 2075 46 1208 1308
Fragments arranged by size:
2075 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 308 410 1651 2429 2619 3411 308
Size: 102 1241 778 190 792 1534
Fragments arranged by size:
1534 1241 792 778 190 102
BsmI GAATG_Cn'
Cuts at: 439 974 439
Size: 535 4102
Bsp1286I G_dGCh'C
Cuts at: 139 308 410 1420 1651 2262 2355 2429 2619
Size: 169 102 1010 231 611 93 74 190
Cuts at: 2619 2681 3411 139
Size: 62 730 1365
Fragments arranged by size:
1365 1010 730 611 231 190 169 102
93 74 62
BspEI T'CCGG_A
Cuts at: 1077 1109 3857 1077
Size: 32 2748 1857
Fragments arranged by size:
2748 1857 32
BspHI T'CATG_A
Cuts at: 3813 3813
Size: 4637
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1687 1901 2203 2584 1687
Size: 214 302 381 3740
Fragments arranged by size:
3740 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1503 2929 2983 4467 4632 50
Size: 1453 1426 54 1484 165 55
Fragments arranged by size:
1484 1453 1426 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2549 2549
Size: 4637
BsrFI r'CCGG_y
Cuts at: 453 2635 2816 3025 453
Size: 2182 181 209 2065
Fragments arranged by size:
2182 2065 209 181
BsrGI T'GTAC_A
Cuts at: 546 546
Size: 4637
BssSI C'ACGA_G
Cuts at: 119 2908 3266 119
Size: 2789 358 1490
Fragments arranged by size:
2789 1490 358
BstAPI GCAn_nnn'nTGC
Cuts at: 2014 2014
Size: 4637
BstBI TT'CG_AA
Cuts at: 2998 2998
Size: 4637
BstXI CCAn_nnnn'nTGG
Cuts at: 1401 1401
Size: 4637
BstYI r'GATC_y
Cuts at: 1 111 554 1662 2129 2152 2487 2733 3745
Size: 110 443 1108 467 23 335 246 1012
Cuts at: 3745 3831 3843 4189 1
Size: 86 12 346 449
Fragments arranged by size:
1108 1012 467 449 443 346 335 246
110 86 23 12
BtgI C'CryG_G
Cuts at: 107 1615 107
Size: 1508 3129
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2587 2768 2931 2587
Size: 181 163 4293
Fragments arranged by size:
4293 181 163
BtsI GCAGTG_nn'
Cuts at: 1880 1880
Size: 4637
DraI TTT'AAA
Cuts at: 590 1636 590
Size: 1046 3591
DrdI GACnn_nn'nnGTC
Cuts at: 2343 3201 2343
Size: 858 3779
EaeI y'GGCC_r
Cuts at: 2222 2396 2787 2814 4159 2222
Size: 174 391 27 1345 2700
Fragments arranged by size:
2700 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2222 2222
Size: 4637
EarI CTCTTCn'nnn_
Cuts at: 142 486 1172 2660 2870 142
Size: 344 686 1488 210 1909
Fragments arranged by size:
1909 1488 686 344 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2845 3155 3301 4447 2845
Size: 310 146 1146 3035
Fragments arranged by size:
3035 1146 310 146
EcoICRI GAG'CTC
Cuts at: 306 408 1649 306
Size: 102 1241 3294
Fragments arranged by size:
3294 1241 102
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 382 833 907 2462 2894 3641 3909 382
Size: 451 74 1555 432 747 268 1110
Fragments arranged by size:
1555 1110 747 451 432 268 74
EcoO109I rG'GnC_Cy
Cuts at: 520 1776 520
Size: 1256 3381
EcoRI G'AATT_C
Cuts at: 746 1641 746
Size: 895 3742
EcoRV GAT'ATC
Cuts at: 844 844
Size: 4637
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 602 1201 2656 602
Size: 599 1455 2583
Fragments arranged by size:
2583 1455 599
FspI TGC'GCA
Cuts at: 1589 2418 1589
Size: 829 3808
HaeII r_GCGC'y
Cuts at: 1488 2071 2319 3341 4320 1488
Size: 583 248 1022 979 1805
Fragments arranged by size:
1805 1022 979 583 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2479 2509 2511 2541 2479
Size: 30 2 30 4575
Fragments arranged by size:
4575 30 30 2
HincII GTy'rAC
Cuts at: 1253 1676 1253
Size: 423 4214
KasI G'GCGC_C
Cuts at: 2067 2315 2067
Size: 248 4389
KpnI G_GTAC'C
Cuts at: 1657 1657
Size: 4637
MfeI C'AATT_G
Cuts at: 539 539
Size: 4637
MluI A'CGCG_T
Cuts at: 4070 4070
Size: 4637
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 923 3308 3492 923
Size: 2385 184 2068
Fragments arranged by size:
2385 2068 184
MscI TGG'CCA
Cuts at: 2398 2398
Size: 4637
MslI CAynn'nnrTG
Cuts at: 727 1399 2626 2753 727
Size: 672 1227 127 2611
Fragments arranged by size:
2611 1227 672 127
MspA1I CmG'CkG
Cuts at: 1010 1367 1748 2062 2422 3435 3680 1010
Size: 357 381 314 360 1013 245 1967
Fragments arranged by size:
1967 1013 381 360 357 314 245
NaeI GCC'GGC
Cuts at: 2818 2818
Size: 4637
NarI GG'CG_CC
Cuts at: 2068 2316 2068
Size: 248 4389
NcoI C'CATG_G
Cuts at: 107 107
Size: 4637
NgoMIV G'CCGG_C
Cuts at: 2816 2816
Size: 4637
NsiI A_TGCA'T
Cuts at: 439 439
Size: 4637
NspI r_CATG'y
Cuts at: 1690 2190 3097 1690
Size: 500 907 3230
Fragments arranged by size:
3230 907 500
PciI A'CATG_T
Cuts at: 3093 3093
Size: 4637
PmeI GTTT'AAAC
Cuts at: 1636 1636
Size: 4637
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 889 921 3776 3808 889
Size: 32 2855 32 1718
Fragments arranged by size:
2855 1718 32 32
PstI C_TGCA'G
Cuts at: 1684 2369 1684
Size: 685 3952
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4637
PvuII CAG'CTG
Cuts at: 1010 1367 2062 2422 1010
Size: 357 695 360 3225
Fragments arranged by size:
3225 695 360 357
RsrII CG'GwC_CG
Cuts at: 2832 2832
Size: 4637
SacI G_AGCT'C
Cuts at: 308 410 1651 308
Size: 102 1241 3294
Fragments arranged by size:
3294 1241 102
SalI G'TCGA_C
Cuts at: 1674 1674
Size: 4637
SapI GCTCTTCn'nnn_
Cuts at: 1172 2660 2870 1172
Size: 1488 210 2939
Fragments arranged by size:
2939 1488 210
SbfI CC_TGCA'GG
Cuts at: 1684 1684
Size: 4637
ScaI AGT'ACT
Cuts at: 4154 4154
Size: 4637
SfcI C'TryA_G
Cuts at: 33 1680 2365 3358 3549 33
Size: 1647 685 993 191 1121
Fragments arranged by size:
1647 1121 993 685 191
SfoI GGC'GCC
Cuts at: 2069 2317 2069
Size: 248 4389
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4637
SmaI CCC'GGG
Cuts at: 1659 1659
Size: 4637
SmlI C'TyrA_G
Cuts at: 132 188 1792 2707 3199 3461 132
Size: 56 1604 915 492 262 1308
Fragments arranged by size:
1604 1308 915 492 262 56
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4637
SphI G_CATG'C
Cuts at: 1690 2190 1690
Size: 500 4137
StyI C'CwwG_G
Cuts at: 107 764 1771 2125 107
Size: 657 1007 354 2619
Fragments arranged by size:
2619 1007 657 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 337 2220 2353 2812 3021 337
Size: 1883 133 459 209 1953
Fragments arranged by size:
1953 1883 459 209 133
TatI w'GTAC_w
Cuts at: 384 546 4152 384
Size: 162 3606 869
Fragments arranged by size:
3606 869 162
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4154 4186 4154
Size: 32 4605
Tth111I GACn'n_nGTC
Cuts at: 1003 2434 1003
Size: 1431 3206
XbaI T'CTAG_A
Cuts at: 1668 1668
Size: 4637
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2132 2132
Size: 4637
XmaI C'CCGG_G
Cuts at: 1657 1657
Size: 4637
XmnI GAAnn'nnTTC
Cuts at: 286 746 904 286
Size: 460 158 4019
Fragments arranged by size:
4019 460 158
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BcgI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssSI BstAPI BstBI BstXI BstYI BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NsiI NspI
PciI PmeI PpiI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI ApaI
AscI AvrII BaeI BbvCI BmgBI BmtI BsgI BsiWI
BsmBI BssHII BstEII BstZ17I Bsu36I ClaI CspCI DraIII
EcoNI FseI FspAI HindIII HpaI NdeI NheI NotI
NruI PacI PasI PflMI PfoI PmlI PpuMI PshAI
PsiI PspOMI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI SspI StuI SwaI XhoI ZraI