Restriction map for
pF1KB5929
(Circular) (Six-base) MAPSORT of: pF1KB5929.seq Check: 468 from: 1 to: 4634
pF1KB5929 4634 bp
With 182 enzymes: SgfI *
October 7, 2009 14:18 ..
AccI GT'mk_AC
Cuts at: 1672 1672
Size: 4634
Acc65I G'GTAC_C
Cuts at: 145 1650 145
Size: 1505 3129
AclI AA'CG_TT
Cuts at: 4474 4474
Size: 4634
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 402 1138 1193 1475 2459 2891 3638 3906 402
Size: 736 55 282 984 432 747 268 1130
Fragments arranged by size:
1130 984 747 736 432 282 268 55
AflIII A'CryG_T
Cuts at: 1986 3090 4067 1986
Size: 1104 977 2553
Fragments arranged by size:
2553 1104 977
AgeI A'CCGG_T
Cuts at: 3022 3022
Size: 4634
AlwNI CAG_nnn'CTG
Cuts at: 217 1939 3506 217
Size: 1722 1567 1345
Fragments arranged by size:
1722 1567 1345
ApaLI G'TGCA_C
Cuts at: 3404 3404
Size: 4634
ApoI r'AATT_y
Cuts at: 595 1289 1638 3987 4243 595
Size: 694 349 2349 256 986
Fragments arranged by size:
2349 986 694 349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4634
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4634
AvaI C'yCGr_G
Cuts at: 132 1654 132
Size: 1522 3112
BamHI G'GATC_C
Cuts at: 1659 1659
Size: 4634
BanI G'GyrC_C
Cuts at: 136 145 1650 2064 2312 2347 136
Size: 9 1505 414 248 35 2423
Fragments arranged by size:
2423 1505 414 248 35 9
BanII G_rGCy'C
Cuts at: 129 1648 2678 129
Size: 1519 1030 2085
Fragments arranged by size:
2085 1519 1030
BbeI G_GCGC'C
Cuts at: 2068 2316 2068
Size: 248 4386
BbsI GAAGACnn'nnnn_
Cuts at: 1119 1119
Size: 4634
BciVI GTATCCnnnnn_n'
Cuts at: 1344 2532 3293 4634 1344
Size: 1188 761 1341 1344
Fragments arranged by size:
1344 1341 1188 761
BclI T'GATC_A
Cuts at: 813 2154 813
Size: 1341 3293
BglI GCCn_nnn'nGGC
Cuts at: 163 1318 163
Size: 1155 3479
BglII A'GATC_T
Cuts at: 1450 1479 2149 4186 1450
Size: 29 670 2037 1898
Fragments arranged by size:
2037 1898 670 29
BlpI GC'TnA_GC
Cuts at: 1746 1746
Size: 4634
Bme1580I G_kGCm'C
Cuts at: 139 796 2259 2352 3408 139
Size: 657 1463 93 1056 1365
Fragments arranged by size:
1463 1365 1056 657 93
BmrI ACTGGGnnnn_n'
Cuts at: 370 438 647 1846 1927 2262 4406 370
Size: 68 209 1199 81 335 2144 598
Fragments arranged by size:
2144 1199 598 335 209 81 68
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3001 3001
Size: 4634
Bpu10I CC'TnA_GC
Cuts at: 1528 1528
Size: 4634
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1810 2689 3181 3479 1810
Size: 879 492 298 2965
Fragments arranged by size:
2965 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 418 4338 418
Size: 3920 714
BsaAI yAC'GTr
Cuts at: 1987 1987
Size: 4634
BsaBI GATnn'nnATC
Cuts at: 6 1478 1543 2148 4185 6
Size: 1472 65 605 2037 455
Fragments arranged by size:
2037 1472 605 455 65
BsaHI Gr'CG_yC
Cuts at: 2065 2313 3015 4511 2065
Size: 248 702 1496 2188
Fragments arranged by size:
2188 1496 702 248
BsaWI w'CCGG_w
Cuts at: 142 1823 2344 3022 3296 3443 3854 142
Size: 1681 521 678 274 147 411 922
Fragments arranged by size:
1681 922 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 420 450 420
Size: 30 4604
BseYI C'CCAG_C
Cuts at: 2059 2817 3394 3890 2059
Size: 758 577 496 2803
Fragments arranged by size:
2803 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 962 962
Size: 4634
BsiEI CG_ry'CG
Cuts at: 104 2176 2222 3430 104
Size: 2072 46 1208 1308
Fragments arranged by size:
2072 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 477 1648 2426 2616 3408 477
Size: 1171 778 190 792 1703
Fragments arranged by size:
1703 1171 792 778 190
BsmI GAATG_Cn'
Cuts at: 220 220
Size: 4634
Bsp1286I G_dGCh'C
Cuts at: 129 139 477 796 1648 2259 2352 2426 2616
Size: 10 338 319 852 611 93 74 190
Cuts at: 2616 2678 3408 129
Size: 62 730 1355
Fragments arranged by size:
1355 852 730 611 338 319 190 93
74 62 10
BspEI T'CCGG_A
Cuts at: 3854 3854
Size: 4634
BspHI T'CATG_A
Cuts at: 3810 3810
Size: 4634
BspMI ACCTGCnnnn'nnnn_
Cuts at: 202 1684 1898 2200 2581 202
Size: 1482 214 302 381 2255
Fragments arranged by size:
2255 1482 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2926 2980 4464 4629 50
Size: 2876 54 1484 165 55
Fragments arranged by size:
2876 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 742 802 2546 742
Size: 60 1744 2830
Fragments arranged by size:
2830 1744 60
BsrFI r'CCGG_y
Cuts at: 2632 2813 3022 2632
Size: 181 209 4244
Fragments arranged by size:
4244 209 181
BssSI C'ACGA_G
Cuts at: 2905 3263 2905
Size: 358 4276
BstAPI GCAn_nnn'nTGC
Cuts at: 217 2011 217
Size: 1794 2840
BstBI TT'CG_AA
Cuts at: 714 2995 714
Size: 2281 2353
BstYI r'GATC_y
Cuts at: 1 1248 1450 1479 1659 2126 2149 2484 2730
Size: 1247 202 29 180 467 23 335 246
Cuts at: 2730 3742 3828 3840 4186 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1247 1012 467 449 346 335 246 202
180 86 29 23 12
BtgI C'CryG_G
Cuts at: 107 161 170 107
Size: 54 9 4571
Fragments arranged by size:
4571 54 9
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2584 2765 2928 2584
Size: 181 163 4290
Fragments arranged by size:
4290 181 163
BtsI GCAGTG_nn'
Cuts at: 1877 1877
Size: 4634
DraI TTT'AAA
Cuts at: 1633 1633
Size: 4634
DrdI GACnn_nn'nnGTC
Cuts at: 2340 3198 2340
Size: 858 3776
EaeI y'GGCC_r
Cuts at: 2219 2393 2784 2811 4156 2219
Size: 174 391 27 1345 2697
Fragments arranged by size:
2697 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2219 2219
Size: 4634
EarI CTCTTCn'nnn_
Cuts at: 421 864 1294 1322 2657 2867 421
Size: 443 430 28 1335 210 2188
Fragments arranged by size:
2188 1335 443 430 210 28
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 575 2842 3152 3298 4444 575
Size: 2267 310 146 1146 765
Fragments arranged by size:
2267 1146 765 310 146
EcoICRI GAG'CTC
Cuts at: 1646 1646
Size: 4634
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 402 1138 1193 1475 2459 2891 3638 3906 402
Size: 736 55 282 984 432 747 268 1130
Fragments arranged by size:
1130 984 747 736 432 282 268 55
EcoO109I rG'GnC_Cy
Cuts at: 1272 1773 1272
Size: 501 4133
EcoRI G'AATT_C
Cuts at: 595 1638 595
Size: 1043 3591
EcoRV GAT'ATC
Cuts at: 1164 1164
Size: 4634
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2653 2653
Size: 4634
FspI TGC'GCA
Cuts at: 2415 2415
Size: 4634
HaeII r_GCGC'y
Cuts at: 2068 2316 3338 4317 2068
Size: 248 1022 979 2385
Fragments arranged by size:
2385 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1148 1153 1180 1185 1416 1448 1604 1636 2476
Size: 5 27 5 231 32 156 32 840
Cuts at: 2476 2506 2508 2538 1148
Size: 30 2 30 3244
Fragments arranged by size:
3244 840 231 156 32 32 30 30
27 5 5 2
HincII GTy'rAC
Cuts at: 1673 1673
Size: 4634
KasI G'GCGC_C
Cuts at: 2064 2312 2064
Size: 248 4386
KpnI G_GTAC'C
Cuts at: 149 1654 149
Size: 1505 3129
MfeI C'AATT_G
Cuts at: 925 925
Size: 4634
MluI A'CGCG_T
Cuts at: 4067 4067
Size: 4634
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 473 511 3305 3489 473
Size: 38 2794 184 1618
Fragments arranged by size:
2794 1618 184 38
MscI TGG'CCA
Cuts at: 2395 2395
Size: 4634
MslI CAynn'nnrTG
Cuts at: 2623 2750 2623
Size: 127 4507
MspA1I CmG'CkG
Cuts at: 151 163 172 456 1347 1745 2059 2419 3432
Size: 12 9 284 891 398 314 360 1013
Cuts at: 3432 3677 151
Size: 245 1108
Fragments arranged by size:
1108 1013 891 398 360 314 284 245
12 9
NaeI GCC'GGC
Cuts at: 2815 2815
Size: 4634
NarI GG'CG_CC
Cuts at: 2065 2313 2065
Size: 248 4386
NcoI C'CATG_G
Cuts at: 107 107
Size: 4634
NgoMIV G'CCGG_C
Cuts at: 2813 2813
Size: 4634
NspI r_CATG'y
Cuts at: 651 1687 2187 3094 651
Size: 1036 500 907 2191
Fragments arranged by size:
2191 1036 907 500
PciI A'CATG_T
Cuts at: 3090 3090
Size: 4634
PmeI GTTT'AAAC
Cuts at: 1633 1633
Size: 4634
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3773 3805 3773
Size: 32 4602
PpuMI rG'GwC_Cy
Cuts at: 1272 1272
Size: 4634
PstI C_TGCA'G
Cuts at: 156 213 542 1681 2366 156
Size: 57 329 1139 685 2424
Fragments arranged by size:
2424 1139 685 329 57
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4634
PvuII CAG'CTG
Cuts at: 456 1347 2059 2419 456
Size: 891 712 360 2671
Fragments arranged by size:
2671 891 712 360
RsrII CG'GwC_CG
Cuts at: 2829 2829
Size: 4634
SacI G_AGCT'C
Cuts at: 1648 1648
Size: 4634
SacII CC_GC'GG
Cuts at: 164 173 164
Size: 9 4625
SalI G'TCGA_C
Cuts at: 1671 1671
Size: 4634
SapI GCTCTTCn'nnn_
Cuts at: 1322 2657 2867 1322
Size: 1335 210 3089
Fragments arranged by size:
3089 1335 210
SbfI CC_TGCA'GG
Cuts at: 1681 1681
Size: 4634
ScaI AGT'ACT
Cuts at: 330 4151 330
Size: 3821 813
SfcI C'TryA_G
Cuts at: 33 152 209 538 1211 1677 2362 3355 3546
Size: 119 57 329 673 466 685 993 191
Cuts at: 3546 33
Size: 1121
Fragments arranged by size:
1121 993 685 673 466 329 191 119
57
SfoI GGC'GCC
Cuts at: 2066 2314 2066
Size: 248 4386
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4634
SmaI CCC'GGG
Cuts at: 1656 1656
Size: 4634
SmlI C'TyrA_G
Cuts at: 1789 2704 3196 3458 1789
Size: 915 492 262 2965
Fragments arranged by size:
2965 915 492 262
SpeI A'CTAG_T
Cuts at: 67 325 67
Size: 258 4376
SphI G_CATG'C
Cuts at: 651 1687 2187 651
Size: 1036 500 3098
Fragments arranged by size:
3098 1036 500
SspI AAT'ATT
Cuts at: 582 582
Size: 4634
StuI AGG'CCT
Cuts at: 544 1526 544
Size: 982 3652
StyI C'CwwG_G
Cuts at: 107 1043 1768 2122 107
Size: 936 725 354 2619
Fragments arranged by size:
2619 936 725 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2217 2350 2809 3018 2217
Size: 133 459 209 3833
Fragments arranged by size:
3833 459 209 133
TatI w'GTAC_w
Cuts at: 328 4149 328
Size: 3821 813
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4151 4183 4151
Size: 32 4602
Tth111I GACn'n_nGTC
Cuts at: 2431 2431
Size: 4634
XbaI T'CTAG_A
Cuts at: 1560 1665 1560
Size: 105 4529
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1612 2129 1612
Size: 517 4117
XmaI C'CCGG_G
Cuts at: 1654 1654
Size: 4634
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BciVI BclI BglI BglII BlpI Bme1580I BmrI
BplI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PciI PmeI PpiI PpuMI PstI PvuI PvuII
RsrII SacI SacII SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbvCI BcgI BfrBI BmgBI
BmtI BpmI BseRI BsiWI BsmBI BsrGI BssHII BstEII
BstXI BstZ17I Bsu36I ClaI CspCI DraIII EcoNI FseI
FspAI HindIII HpaI NdeI NheI NotI NruI NsiI
PacI PasI PflMI PfoI PmlI PshAI PsiI PspOMI
PspXI PsrI SanDI SexAI SfiI SgrAI SnaBI SrfI
SwaI XhoI XmnI ZraI