Restriction map for
pF1KB5924
(Circular) (Six-base) MAPSORT of: pF1KB5924.seq Check: 8034 from: 1 to: 4673
pF1KB5924 4673 bp
With 182 enzymes: SgfI *
December 27, 2011 16:29 ..
AccI GT'mk_AC
Cuts at: 824 1106 1711 824
Size: 282 605 3786
Fragments arranged by size:
3786 605 282
Acc65I G'GTAC_C
Cuts at: 1689 1689
Size: 4673
AclI AA'CG_TT
Cuts at: 4513 4513
Size: 4673
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 938 1325 1553 2498 2930 3677 3945 938
Size: 387 228 945 432 747 268 1666
Fragments arranged by size:
1666 945 747 432 387 268 228
AflIII A'CryG_T
Cuts at: 289 1350 2025 3129 4106 289
Size: 1061 675 1104 977 856
Fragments arranged by size:
1104 1061 977 856 675
AgeI A'CCGG_T
Cuts at: 3061 3061
Size: 4673
AhdI GACnn_n'nnGTC
Cuts at: 642 642
Size: 4673
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1394 1394
Size: 4673
AlwNI CAG_nnn'CTG
Cuts at: 381 1531 1978 3545 381
Size: 1150 447 1567 1509
Fragments arranged by size:
1567 1509 1150 447
ApaLI G'TGCA_C
Cuts at: 3443 3443
Size: 4673
ApoI r'AATT_y
Cuts at: 208 571 1146 1252 1384 1677 4026 4282 208
Size: 363 575 106 132 293 2349 256 599
Fragments arranged by size:
2349 599 575 363 293 256 132 106
AseI AT'TA_AT
Cuts at: 20 20
Size: 4673
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4673
AvaI C'yCGr_G
Cuts at: 1229 1693 1229
Size: 464 4209
BamHI G'GATC_C
Cuts at: 1698 1698
Size: 4673
BanI G'GyrC_C
Cuts at: 1689 2103 2351 2386 1689
Size: 414 248 35 3976
Fragments arranged by size:
3976 414 248 35
BanII G_rGCy'C
Cuts at: 665 1236 1687 2717 665
Size: 571 451 1030 2621
Fragments arranged by size:
2621 1030 571 451
BbeI G_GCGC'C
Cuts at: 2107 2355 2107
Size: 248 4425
BbsI GAAGACnn'nnnn_
Cuts at: 926 926
Size: 4673
BciVI GTATCCnnnnn_n'
Cuts at: 2571 3332 4673 2571
Size: 761 1341 2571
Fragments arranged by size:
2571 1341 761
BclI T'GATC_A
Cuts at: 2193 2193
Size: 4673
BglI GCCn_nnn'nGGC
Cuts at: 793 1030 793
Size: 237 4436
BglII A'GATC_T
Cuts at: 689 2188 4225 689
Size: 1499 2037 1137
Fragments arranged by size:
2037 1499 1137
BlpI GC'TnA_GC
Cuts at: 508 1785 508
Size: 1277 3396
Bme1580I G_kGCm'C
Cuts at: 2298 2391 3447 2298
Size: 93 1056 3524
Fragments arranged by size:
3524 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 436 1049 1885 1966 2301 4445 436
Size: 613 836 81 335 2144 664
Fragments arranged by size:
2144 836 664 613 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 872 1671 3040 872
Size: 799 1369 2505
Fragments arranged by size:
2505 1369 799
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 312 649 1184 1505 312
Size: 337 535 321 3480
Fragments arranged by size:
3480 535 337 321
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1849 2728 3220 3518 1849
Size: 879 492 298 3004
Fragments arranged by size:
3004 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1642 4377 1642
Size: 2735 1938
BsaAI yAC'GTr
Cuts at: 2026 2026
Size: 4673
BsaBI GATnn'nnATC
Cuts at: 6 857 2187 4224 6
Size: 851 1330 2037 455
Fragments arranged by size:
2037 1330 851 455
BsaHI Gr'CG_yC
Cuts at: 2104 2352 3054 4550 2104
Size: 248 702 1496 2227
Fragments arranged by size:
2227 1496 702 248
BsaWI w'CCGG_w
Cuts at: 566 1862 2383 3061 3335 3482 3893 566
Size: 1296 521 678 274 147 411 1346
Fragments arranged by size:
1346 1296 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 130 749 1508 1576 130
Size: 619 759 68 3227
Fragments arranged by size:
3227 759 619 68
BseYI C'CCAG_C
Cuts at: 2098 2856 3433 3929 2098
Size: 758 577 496 2842
Fragments arranged by size:
2842 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 2215 2261 3469 104
Size: 2111 46 1208 1308
Fragments arranged by size:
2111 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 665 1687 2465 2655 3447 665
Size: 1022 778 190 792 1891
Fragments arranged by size:
1891 1022 792 778 190
BsmI GAATG_Cn'
Cuts at: 320 1452 320
Size: 1132 3541
Bsp1286I G_dGCh'C
Cuts at: 665 1236 1687 2298 2391 2465 2655 2717 3447
Size: 571 451 611 93 74 190 62 730
Cuts at: 3447 665
Size: 1891
Fragments arranged by size:
1891 730 611 571 451 190 93 74
62
BspEI T'CCGG_A
Cuts at: 3893 3893
Size: 4673
BspHI T'CATG_A
Cuts at: 1343 3849 1343
Size: 2506 2167
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1723 1937 2239 2620 1723
Size: 214 302 381 3776
Fragments arranged by size:
3776 381 302 214
BsrBI CCG'CTC
Cuts at: 50 165 2965 3019 4503 4668 50
Size: 115 2800 54 1484 165 55
Fragments arranged by size:
2800 1484 165 115 55 54
BsrDI GCAATG_nn'
Cuts at: 951 972 2585 951
Size: 21 1613 3039
Fragments arranged by size:
3039 1613 21
BsrFI r'CCGG_y
Cuts at: 142 2671 2852 3061 142
Size: 2529 181 209 1754
Fragments arranged by size:
2529 1754 209 181
BssHII G'CGCG_C
Cuts at: 120 120
Size: 4673
BssSI C'ACGA_G
Cuts at: 2944 3302 2944
Size: 358 4315
BstAPI GCAn_nnn'nTGC
Cuts at: 381 2050 381
Size: 1669 3004
BstBI TT'CG_AA
Cuts at: 3034 3034
Size: 4673
BstYI r'GATC_y
Cuts at: 1 689 1698 2165 2188 2523 2769 3781 3867
Size: 688 1009 467 23 335 246 1012 86
Cuts at: 3867 3879 4225 1
Size: 12 346 449
Fragments arranged by size:
1012 1009 688 467 449 346 335 246
86 23 12
BstZ17I GTA'TAC
Cuts at: 1107 1107
Size: 4673
Bsu36I CC'TnA_GG
Cuts at: 172 770 172
Size: 598 4075
BtgI C'CryG_G
Cuts at: 107 1100 107
Size: 993 3680
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2623 2804 2967 2623
Size: 181 163 4329
Fragments arranged by size:
4329 181 163
BtsI GCAGTG_nn'
Cuts at: 1916 1916
Size: 4673
DraI TTT'AAA
Cuts at: 261 1672 261
Size: 1411 3262
DrdI GACnn_nn'nnGTC
Cuts at: 1363 2379 3237 1363
Size: 1016 858 2799
Fragments arranged by size:
2799 1016 858
EaeI y'GGCC_r
Cuts at: 737 1466 2258 2432 2823 2850 4195 737
Size: 729 792 174 391 27 1345 1215
Fragments arranged by size:
1345 1215 792 729 391 174 27
EagI C'GGCC_G
Cuts at: 2258 2258
Size: 4673
EarI CTCTTCn'nnn_
Cuts at: 616 814 926 1153 1485 1640 2696 2906 616
Size: 198 112 227 332 155 1056 210 2383
Fragments arranged by size:
2383 1056 332 227 210 198 155 112
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2881 3191 3337 4483 2881
Size: 310 146 1146 3071
Fragments arranged by size:
3071 1146 310 146
EcoICRI GAG'CTC
Cuts at: 663 1685 663
Size: 1022 3651
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 312 649 938 1184 1325 1505 1553 2498 2930
Size: 337 289 246 141 180 48 945 432
Cuts at: 2930 3677 3945 312
Size: 747 268 1040
Fragments arranged by size:
1040 945 747 432 337 289 268 246
180 141 48
EcoNI CCTnn'n_nnAGG
Cuts at: 271 271
Size: 4673
EcoO109I rG'GnC_Cy
Cuts at: 168 795 1473 1812 168
Size: 627 678 339 3029
Fragments arranged by size:
3029 678 627 339
EcoRI G'AATT_C
Cuts at: 1384 1677 1384
Size: 293 4380
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2692 2692
Size: 4673
FspI TGC'GCA
Cuts at: 2454 2454
Size: 4673
HaeII r_GCGC'y
Cuts at: 2107 2355 3377 4356 2107
Size: 248 1022 979 2424
Fragments arranged by size:
2424 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 415 447 628 660 718 746 750 778 2515
Size: 32 181 32 58 28 4 28 1737
Cuts at: 2515 2545 2547 2577 415
Size: 30 2 30 2511
Fragments arranged by size:
2511 1737 181 58 32 32 30 30
28 28 4 2
HincII GTy'rAC
Cuts at: 954 1007 1356 1712 954
Size: 53 349 356 3915
Fragments arranged by size:
3915 356 349 53
HpaI GTT'AAC
Cuts at: 1007 1007
Size: 4673
KasI G'GCGC_C
Cuts at: 2103 2351 2103
Size: 248 4425
KpnI G_GTAC'C
Cuts at: 1693 1693
Size: 4673
MfeI C'AATT_G
Cuts at: 649 649
Size: 4673
MluI A'CGCG_T
Cuts at: 4106 4106
Size: 4673
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 811 937 3344 3528 811
Size: 126 2407 184 1956
Fragments arranged by size:
2407 1956 184 126
MscI TGG'CCA
Cuts at: 2434 2434
Size: 4673
MslI CAynn'nnrTG
Cuts at: 462 944 2662 2789 462
Size: 482 1718 127 2346
Fragments arranged by size:
2346 1718 482 127
MspA1I CmG'CkG
Cuts at: 678 880 1069 1784 2098 2458 3471 3716 678
Size: 202 189 715 314 360 1013 245 1635
Fragments arranged by size:
1635 1013 715 360 314 245 202 189
NaeI GCC'GGC
Cuts at: 144 2854 144
Size: 2710 1963
NarI GG'CG_CC
Cuts at: 2104 2352 2104
Size: 248 4425
NcoI C'CATG_G
Cuts at: 107 1100 107
Size: 993 3680
NgoMIV G'CCGG_C
Cuts at: 142 2852 142
Size: 2710 1963
NspI r_CATG'y
Cuts at: 293 1354 1726 2226 3133 293
Size: 1061 372 500 907 1833
Fragments arranged by size:
1833 1061 907 500 372
PciI A'CATG_T
Cuts at: 289 1350 3129 289
Size: 1061 1779 1833
Fragments arranged by size:
1833 1779 1061
PmeI GTTT'AAAC
Cuts at: 1672 1672
Size: 4673
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3812 3844 3812
Size: 32 4641
PpuMI rG'GwC_Cy
Cuts at: 168 168
Size: 4673
PsiI TTA'TAA
Cuts at: 271 271
Size: 4673
PstI C_TGCA'G
Cuts at: 1455 1720 2405 1455
Size: 265 685 3723
Fragments arranged by size:
3723 685 265
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4673
PvuII CAG'CTG
Cuts at: 880 1069 2098 2458 880
Size: 189 1029 360 3095
Fragments arranged by size:
3095 1029 360 189
RsrII CG'GwC_CG
Cuts at: 2868 2868
Size: 4673
SacI G_AGCT'C
Cuts at: 665 1687 665
Size: 1022 3651
SalI G'TCGA_C
Cuts at: 1710 1710
Size: 4673
SapI GCTCTTCn'nnn_
Cuts at: 616 926 2696 2906 616
Size: 310 1770 210 2383
Fragments arranged by size:
2383 1770 310 210
SbfI CC_TGCA'GG
Cuts at: 1720 1720
Size: 4673
ScaI AGT'ACT
Cuts at: 314 1633 4190 314
Size: 1319 2557 797
Fragments arranged by size:
2557 1319 797
SfcI C'TryA_G
Cuts at: 33 1182 1451 1716 2401 3394 3585 33
Size: 1149 269 265 685 993 191 1121
Fragments arranged by size:
1149 1121 993 685 269 265 191
SfoI GGC'GCC
Cuts at: 2105 2353 2105
Size: 248 4425
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4673
SmaI CCC'GGG
Cuts at: 1695 1695
Size: 4673
SmlI C'TyrA_G
Cuts at: 1828 2743 3235 3497 1828
Size: 915 492 262 3004
Fragments arranged by size:
3004 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4673
SphI G_CATG'C
Cuts at: 1726 2226 1726
Size: 500 4173
StyI C'CwwG_G
Cuts at: 107 1100 1359 1807 2161 107
Size: 993 259 448 354 2619
Fragments arranged by size:
2619 993 448 354 259
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 736 2256 2389 2848 3057 736
Size: 1520 133 459 209 2352
Fragments arranged by size:
2352 1520 459 209 133
TatI w'GTAC_w
Cuts at: 312 1631 4188 312
Size: 1319 2557 797
Fragments arranged by size:
2557 1319 797
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4190 4222 4190
Size: 32 4641
Tth111I GACn'n_nGTC
Cuts at: 2470 2470
Size: 4673
XbaI T'CTAG_A
Cuts at: 1041 1704 1041
Size: 663 4010
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1476 2168 1476
Size: 692 3981
XmaI C'CCGG_G
Cuts at: 1693 1693
Size: 4673
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BglI BglII BlpI
Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI
BstAPI BstBI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PciI PmeI
PpiI PpuMI PsiI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AleI AloI ApaI AscI
AvrII BaeI BbvCI BcgI BfrBI BmgBI BmtI Bpu10I
BsaXI BsgI BsiWI BsmBI BsrGI BstEII BstXI ClaI
CspCI DraIII EcoRV FseI FspAI HindIII NdeI NheI
NotI NruI NsiI PacI PasI PflMI PfoI PmlI
PshAI PspOMI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI SspI StuI SwaI XhoI XmnI
ZraI