Restriction map for
pF1KB5908
(Circular) (Six-base) MAPSORT of: pF1KB5908.seq Check: 548 from: 1 to: 4673
pF1KB5908 4673 bp
With 182 enzymes: SgfI *
October 7, 2009 14:18 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 429 429
Size: 4673
AccI GT'mk_AC
Cuts at: 1711 1711
Size: 4673
Acc65I G'GTAC_C
Cuts at: 1689 1689
Size: 4673
AclI AA'CG_TT
Cuts at: 4513 4513
Size: 4673
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 223 1013 1381 2498 2930 3677 3945 223
Size: 790 368 1117 432 747 268 951
Fragments arranged by size:
1117 951 790 747 432 368 268
AflII C'TTAA_G
Cuts at: 457 457
Size: 4673
AflIII A'CryG_T
Cuts at: 2025 3129 4106 2025
Size: 1104 977 2592
Fragments arranged by size:
2592 1104 977
AgeI A'CCGG_T
Cuts at: 618 3061 618
Size: 2443 2230
AleI CACnn'nnGTG
Cuts at: 439 1664 439
Size: 1225 3448
AlwNI CAG_nnn'CTG
Cuts at: 345 991 1978 3545 345
Size: 646 987 1567 1473
Fragments arranged by size:
1567 1473 987 646
ApaLI G'TGCA_C
Cuts at: 3443 3443
Size: 4673
ApoI r'AATT_y
Cuts at: 1677 4026 4282 1677
Size: 2349 256 2068
Fragments arranged by size:
2349 2068 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4673
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4673
AvaI C'yCGr_G
Cuts at: 879 1286 1693 879
Size: 407 407 3859
Fragments arranged by size:
3859 407 407
BamHI G'GATC_C
Cuts at: 843 1522 1698 843
Size: 679 176 3818
Fragments arranged by size:
3818 679 176
BanI G'GyrC_C
Cuts at: 441 621 1447 1689 2103 2351 2386 441
Size: 180 826 242 414 248 35 2728
Fragments arranged by size:
2728 826 414 248 242 180 35
BanII G_rGCy'C
Cuts at: 170 983 1293 1687 2717 170
Size: 813 310 394 1030 2126
Fragments arranged by size:
2126 1030 813 394 310
BbeI G_GCGC'C
Cuts at: 2107 2355 2107
Size: 248 4425
BbsI GAAGACnn'nnnn_
Cuts at: 1541 1541
Size: 4673
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4639
BciVI GTATCCnnnnn_n'
Cuts at: 2571 3332 4673 2571
Size: 761 1341 2571
Fragments arranged by size:
2571 1341 761
BclI T'GATC_A
Cuts at: 2193 2193
Size: 4673
BglII A'GATC_T
Cuts at: 2188 4225 2188
Size: 2037 2636
BlpI GC'TnA_GC
Cuts at: 1785 1785
Size: 4673
Bme1580I G_kGCm'C
Cuts at: 380 416 736 2298 2391 3447 380
Size: 36 320 1562 93 1056 1606
Fragments arranged by size:
1606 1562 1056 320 93 36
BmrI ACTGGGnnnn_n'
Cuts at: 351 1487 1885 1966 2301 4445 351
Size: 1136 398 81 335 2144 579
Fragments arranged by size:
2144 1136 579 398 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3040 3040
Size: 4673
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 243 1252 1316 243
Size: 1009 64 3600
Fragments arranged by size:
3600 1009 64
Bpu10I CC'TnA_GC
Cuts at: 574 574
Size: 4673
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1393 1849 2728 3220 3518 1393
Size: 456 879 492 298 2548
Fragments arranged by size:
2548 879 492 456 298
BsaI GGTCTCn'nnnn_
Cuts at: 1327 4377 1327
Size: 3050 1623
BsaAI yAC'GTr
Cuts at: 2026 2026
Size: 4673
BsaBI GATnn'nnATC
Cuts at: 6 2187 4224 6
Size: 2181 2037 455
Fragments arranged by size:
2181 2037 455
BsaHI Gr'CG_yC
Cuts at: 2104 2352 3054 4550 2104
Size: 248 702 1496 2227
Fragments arranged by size:
2227 1496 702 248
BsaWI w'CCGG_w
Cuts at: 618 1862 2383 3061 3335 3482 3893 618
Size: 1244 521 678 274 147 411 1398
Fragments arranged by size:
1398 1244 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1545 1575 1620 1650 1545
Size: 30 45 30 4568
Fragments arranged by size:
4568 45 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 166 246 1644 166
Size: 80 1398 3195
Fragments arranged by size:
3195 1398 80
BseYI C'CCAG_C
Cuts at: 2098 2856 3433 3929 2098
Size: 758 577 496 2842
Fragments arranged by size:
2842 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 484 484
Size: 4673
BsiEI CG_ry'CG
Cuts at: 104 2215 2261 3469 104
Size: 2111 46 1208 1308
Fragments arranged by size:
2111 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 983 1005 1238 1293 1661 1687 2465 2655 3447
Size: 22 233 55 368 26 778 190 792
Cuts at: 3447 983
Size: 2209
Fragments arranged by size:
2209 792 778 368 233 190 55 26
22
BsmBI CGTCTCn'nnnn_
Cuts at: 1645 1645
Size: 4673
Bsp1286I G_dGCh'C
Cuts at: 170 380 416 736 983 1005 1238 1293 1661
Size: 210 36 320 247 22 233 55 368
Cuts at: 1661 1687 2298 2391 2465 2655 2717 3447 170
Size: 26 611 93 74 190 62 730 1396
Fragments arranged by size:
1396 730 611 368 320 247 233 210
190 93 74 62 55 36 26 22
BspEI T'CCGG_A
Cuts at: 3893 3893
Size: 4673
BspHI T'CATG_A
Cuts at: 159 3849 159
Size: 3690 983
BspMI ACCTGCnnnn'nnnn_
Cuts at: 429 510 1723 1937 2239 2620 429
Size: 81 1213 214 302 381 2482
Fragments arranged by size:
2482 1213 381 302 214 81
BsrBI CCG'CTC
Cuts at: 50 2965 3019 4503 4668 50
Size: 2915 54 1484 165 55
Fragments arranged by size:
2915 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2585 2585
Size: 4673
BsrFI r'CCGG_y
Cuts at: 618 1244 2671 2852 3061 618
Size: 626 1427 181 209 2230
Fragments arranged by size:
2230 1427 626 209 181
BsrGI T'GTAC_A
Cuts at: 1420 1420
Size: 4673
BssSI C'ACGA_G
Cuts at: 755 2944 3302 755
Size: 2189 358 2126
Fragments arranged by size:
2189 2126 358
BstAPI GCAn_nnn'nTGC
Cuts at: 297 345 1254 2050 297
Size: 48 909 796 2920
Fragments arranged by size:
2920 909 796 48
BstBI TT'CG_AA
Cuts at: 140 3034 140
Size: 2894 1779
BstXI CCAn_nnnn'nTGG
Cuts at: 1518 1518
Size: 4673
BstYI r'GATC_y
Cuts at: 1 843 1522 1698 2165 2188 2523 2769 3781
Size: 842 679 176 467 23 335 246 1012
Cuts at: 3781 3867 3879 4225 1
Size: 86 12 346 449
Fragments arranged by size:
1012 842 679 467 449 346 335 246
176 86 23 12
BtgI C'CryG_G
Cuts at: 650 1160 1316 650
Size: 510 156 4007
Fragments arranged by size:
4007 510 156
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2623 2804 2967 2623
Size: 181 163 4329
Fragments arranged by size:
4329 181 163
BtsI GCAGTG_nn'
Cuts at: 1916 1916
Size: 4673
ClaI AT'CG_AT
Cuts at: 788 788
Size: 4673
DraI TTT'AAA
Cuts at: 1672 1672
Size: 4673
DrdI GACnn_nn'nnGTC
Cuts at: 2379 3237 2379
Size: 858 3815
EaeI y'GGCC_r
Cuts at: 1007 2258 2432 2823 2850 4195 1007
Size: 1251 174 391 27 1345 1485
Fragments arranged by size:
1485 1345 1251 391 174 27
EagI C'GGCC_G
Cuts at: 2258 2258
Size: 4673
EarI CTCTTCn'nnn_
Cuts at: 158 199 701 1643 2696 2906 158
Size: 41 502 942 1053 210 1925
Fragments arranged by size:
1925 1053 942 502 210 41
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 207 2881 3191 3337 4483 207
Size: 2674 310 146 1146 397
Fragments arranged by size:
2674 1146 397 310 146
EcoICRI GAG'CTC
Cuts at: 981 1291 1685 981
Size: 310 394 3969
Fragments arranged by size:
3969 394 310
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 223 243 1013 1252 1316 1381 2498 2930 3677
Size: 20 770 239 64 65 1117 432 747
Cuts at: 3677 3945 223
Size: 268 951
Fragments arranged by size:
1117 951 770 747 432 268 239 65
64 20
EcoO109I rG'GnC_Cy
Cuts at: 233 1812 233
Size: 1579 3094
EcoRI G'AATT_C
Cuts at: 1677 1677
Size: 4673
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1411 2692 1411
Size: 1281 3392
FspI TGC'GCA
Cuts at: 2454 2454
Size: 4673
HaeII r_GCGC'y
Cuts at: 2107 2355 3377 4356 2107
Size: 248 1022 979 2424
Fragments arranged by size:
2424 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2515 2545 2547 2577 2515
Size: 30 2 30 4611
Fragments arranged by size:
4611 30 30 2
HincII GTy'rAC
Cuts at: 196 1712 196
Size: 1516 3157
KasI G'GCGC_C
Cuts at: 2103 2351 2103
Size: 248 4425
KpnI G_GTAC'C
Cuts at: 1693 1693
Size: 4673
MluI A'CGCG_T
Cuts at: 4106 4106
Size: 4673
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 698 3344 3528 698
Size: 2646 184 1843
Fragments arranged by size:
2646 1843 184
MscI TGG'CCA
Cuts at: 1009 2434 1009
Size: 1425 3248
MslI CAynn'nnrTG
Cuts at: 439 655 1516 1543 1664 2662 2789 439
Size: 216 861 27 121 998 127 2323
Fragments arranged by size:
2323 998 861 216 127 121 27
MspA1I CmG'CkG
Cuts at: 201 1784 2098 2458 3471 3716 201
Size: 1583 314 360 1013 245 1158
Fragments arranged by size:
1583 1158 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2854 2854
Size: 4673
NarI GG'CG_CC
Cuts at: 2104 2352 2104
Size: 248 4425
NcoI C'CATG_G
Cuts at: 650 650
Size: 4673
NgoMIV G'CCGG_C
Cuts at: 2852 2852
Size: 4673
NspI r_CATG'y
Cuts at: 562 1128 1726 2226 3133 562
Size: 566 598 500 907 2102
Fragments arranged by size:
2102 907 598 566 500
PciI A'CATG_T
Cuts at: 3129 3129
Size: 4673
PmeI GTTT'AAAC
Cuts at: 1672 1672
Size: 4673
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 198 230 3812 3844 198
Size: 32 3582 32 1027
Fragments arranged by size:
3582 1027 32 32
PsiI TTA'TAA
Cuts at: 698 818 698
Size: 120 4553
PspXI vC'TCGA_Gb
Cuts at: 1286 1286
Size: 4673
PstI C_TGCA'G
Cuts at: 350 1384 1720 2405 350
Size: 1034 336 685 2618
Fragments arranged by size:
2618 1034 685 336
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4673
PvuII CAG'CTG
Cuts at: 2098 2458 2098
Size: 360 4313
RsrII CG'GwC_CG
Cuts at: 2868 2868
Size: 4673
SacI G_AGCT'C
Cuts at: 983 1293 1687 983
Size: 310 394 3969
Fragments arranged by size:
3969 394 310
SalI G'TCGA_C
Cuts at: 1710 1710
Size: 4673
SapI GCTCTTCn'nnn_
Cuts at: 158 199 701 2696 2906 158
Size: 41 502 1995 210 1925
Fragments arranged by size:
1995 1925 502 210 41
SbfI CC_TGCA'GG
Cuts at: 1720 1720
Size: 4673
ScaI AGT'ACT
Cuts at: 4190 4190
Size: 4673
SfcI C'TryA_G
Cuts at: 33 346 1380 1500 1716 2401 3394 3585 33
Size: 313 1034 120 216 685 993 191 1121
Fragments arranged by size:
1121 1034 993 685 313 216 191 120
SfoI GGC'GCC
Cuts at: 2105 2353 2105
Size: 248 4425
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4673
SgrAI Cr'CCGG_yG
Cuts at: 618 618
Size: 4673
SmaI CCC'GGG
Cuts at: 1695 1695
Size: 4673
SmlI C'TyrA_G
Cuts at: 457 1286 1372 1828 2743 3235 3497 457
Size: 829 86 456 915 492 262 1633
Fragments arranged by size:
1633 915 829 492 456 262 86
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4673
SphI G_CATG'C
Cuts at: 1726 2226 1726
Size: 500 4173
StuI AGG'CCT
Cuts at: 929 929
Size: 4673
StyI C'CwwG_G
Cuts at: 650 909 1807 2161 650
Size: 259 898 354 3162
Fragments arranged by size:
3162 898 354 259
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 654 2256 2389 2848 3057 654
Size: 1602 133 459 209 2270
Fragments arranged by size:
2270 1602 459 209 133
TatI w'GTAC_w
Cuts at: 1420 4188 1420
Size: 2768 1905
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 408 440 1545 1577 4190 4222 408
Size: 32 1105 32 2613 32 859
Fragments arranged by size:
2613 1105 859 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2470 2470
Size: 4673
XbaI T'CTAG_A
Cuts at: 1704 1704
Size: 4673
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 648 1127 2168 648
Size: 479 1041 3153
Fragments arranged by size:
3153 1041 479
XhoI C'TCGA_G
Cuts at: 1286 1286
Size: 4673
XmaI C'CCGG_G
Cuts at: 1693 1693
Size: 4673
XmnI GAAnn'nnTTC
Cuts at: 1367 1367
Size: 4673
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflII AflIII AgeI
AleI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BcgI BciVI BclI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstXI BstYI
BtgI BtgZI BtsI ClaI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI
PmeI PpiI PsiI PspXI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SgrAI SmaI SmlI SpeI SphI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AhdI AlfI AloI ApaI AscI AvrII
BaeI BbvCI BfrBI BglI BmgBI BmtI BsiWI BsmI
BssHII BstEII BstZ17I Bsu36I CspCI DraIII EcoNI EcoRV
FseI FspAI HindIII HpaI MfeI NdeI NheI NotI
NruI NsiI PacI PasI PflMI PfoI PmlI PpuMI
PshAI PspOMI PsrI SacII SanDI SexAI SfiI SnaBI
SrfI SspI SwaI ZraI