Restriction map for
pF1KB5886
(Circular) (Six-base) MAPSORT of: pF1KB5886.seq Check: 367 from: 1 to: 4604
pF1KB5886 4604 bp
With 182 enzymes: SgfI *
July 15, 2010 18:27 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 608 1179 608
Size: 571 4033
AccI GT'mk_AC
Cuts at: 482 1642 482
Size: 1160 3444
Acc65I G'GTAC_C
Cuts at: 1620 1620
Size: 4604
AclI AA'CG_TT
Cuts at: 4444 4444
Size: 4604
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 581 937 2429 2861 3608 3876 581
Size: 356 1492 432 747 268 1309
Fragments arranged by size:
1492 1309 747 432 356 268
AflIII A'CryG_T
Cuts at: 1956 3060 4037 1956
Size: 1104 977 2523
Fragments arranged by size:
2523 1104 977
AgeI A'CCGG_T
Cuts at: 2992 2992
Size: 4604
AlwNI CAG_nnn'CTG
Cuts at: 939 1023 1909 3476 939
Size: 84 886 1567 2067
Fragments arranged by size:
2067 1567 886 84
ApaLI G'TGCA_C
Cuts at: 3374 3374
Size: 4604
ApoI r'AATT_y
Cuts at: 550 841 1608 3957 4213 550
Size: 291 767 2349 256 941
Fragments arranged by size:
2349 941 767 291 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4604
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4604
AvaI C'yCGr_G
Cuts at: 157 458 728 1624 157
Size: 301 270 896 3137
Fragments arranged by size:
3137 896 301 270
AvrII C'CTAG_G
Cuts at: 882 882
Size: 4604
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1343 1376 1343
Size: 33 4571
BamHI G'GATC_C
Cuts at: 723 1629 723
Size: 906 3698
BanI G'GyrC_C
Cuts at: 1620 2034 2282 2317 1620
Size: 414 248 35 3907
Fragments arranged by size:
3907 414 248 35
BanII G_rGCy'C
Cuts at: 225 284 479 575 643 1151 1178 1239 1424
Size: 59 195 96 68 508 27 61 185
Cuts at: 1424 1618 2648 225
Size: 194 1030 2181
Fragments arranged by size:
2181 1030 508 195 194 185 96 68
61 59 27
BbeI G_GCGC'C
Cuts at: 2038 2286 2038
Size: 248 4356
BbsI GAAGACnn'nnnn_
Cuts at: 925 978 925
Size: 53 4551
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1177 1211 1177
Size: 34 4570
BciVI GTATCCnnnnn_n'
Cuts at: 2502 3263 4604 2502
Size: 761 1341 2502
Fragments arranged by size:
2502 1341 761
BclI T'GATC_A
Cuts at: 2124 2124
Size: 4604
BglI GCCn_nnn'nGGC
Cuts at: 591 1000 1546 591
Size: 409 546 3649
Fragments arranged by size:
3649 546 409
BglII A'GATC_T
Cuts at: 2119 4156 2119
Size: 2037 2567
BlpI GC'TnA_GC
Cuts at: 337 866 1716 337
Size: 529 850 3225
Fragments arranged by size:
3225 850 529
Bme1580I G_kGCm'C
Cuts at: 176 2229 2322 3378 176
Size: 2053 93 1056 1402
Fragments arranged by size:
2053 1402 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 348 530 1149 1816 1897 2232 4376 348
Size: 182 619 667 81 335 2144 576
Fragments arranged by size:
2144 667 619 576 335 182 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2971 2971
Size: 4604
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 724 895 1045 1519 1540 724
Size: 171 150 474 21 3788
Fragments arranged by size:
3788 474 171 150 21
Bpu10I CC'TnA_GC
Cuts at: 1267 1267
Size: 4604
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 382 601 730 1366 1780 2659 3151 3449 382
Size: 219 129 636 414 879 492 298 1537
Fragments arranged by size:
1537 879 636 492 414 298 219 129
BsaI GGTCTCn'nnnn_
Cuts at: 790 4308 790
Size: 3518 1086
BsaAI yAC'GTr
Cuts at: 1053 1957 1053
Size: 904 3700
BsaBI GATnn'nnATC
Cuts at: 6 2118 4155 6
Size: 2112 2037 455
Fragments arranged by size:
2112 2037 455
BsaHI Gr'CG_yC
Cuts at: 2035 2283 2985 4481 2035
Size: 248 702 1496 2158
Fragments arranged by size:
2158 1496 702 248
BsaWI w'CCGG_w
Cuts at: 521 827 1793 2314 2992 3266 3413 3824 521
Size: 306 966 521 678 274 147 411 1301
Fragments arranged by size:
1301 966 678 521 411 306 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 184 214 724 754 1093 1123 1315 1345 184
Size: 30 510 30 339 30 192 30 3443
Fragments arranged by size:
3443 510 339 192 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 141 237 1516 141
Size: 96 1279 3229
Fragments arranged by size:
3229 1279 96
BseYI C'CCAG_C
Cuts at: 995 2029 2787 3364 3860 995
Size: 1034 758 577 496 1739
Fragments arranged by size:
1739 1034 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 310 742 946 1036 1074 310
Size: 432 204 90 38 3840
Fragments arranged by size:
3840 432 204 90 38
BsiEI CG_ry'CG
Cuts at: 104 2146 2192 3400 104
Size: 2042 46 1208 1308
Fragments arranged by size:
2042 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 284 457 851 929 1052 1343 1382 1424 1618
Size: 173 394 78 123 291 39 42 194
Cuts at: 1618 2396 2586 3378 284
Size: 778 190 792 1510
Fragments arranged by size:
1510 792 778 394 291 194 190 173
123 78 42 39
BsmBI CGTCTCn'nnnn_
Cuts at: 106 106
Size: 4604
Bsp1286I G_dGCh'C
Cuts at: 176 225 284 457 479 575 643 851 929
Size: 49 59 173 22 96 68 208 78
Cuts at: 929 1052 1151 1178 1239 1343 1382 1424 1618
Size: 123 99 27 61 104 39 42 194
Cuts at: 1618 2229 2322 2396 2586 2648 3378 176
Size: 611 93 74 190 62 730 1402
Fragments arranged by size:
1402 730 611 208 194 190 173 123
104 99 96 93 78 74 68 62
61 59 49 42 39 27 22
BspEI T'CCGG_A
Cuts at: 521 3824 521
Size: 3303 1301
BspHI T'CATG_A
Cuts at: 3780 3780
Size: 4604
BspMI ACCTGCnnnn'nnnn_
Cuts at: 608 1096 1179 1654 1868 2170 2551 608
Size: 488 83 475 214 302 381 2661
Fragments arranged by size:
2661 488 475 381 302 214 83
BsrBI CCG'CTC
Cuts at: 50 236 1233 2896 2950 4434 4599 50
Size: 186 997 1663 54 1484 165 55
Fragments arranged by size:
1663 1484 997 186 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2516 2516
Size: 4604
BsrFI r'CCGG_y
Cuts at: 312 1517 2602 2783 2992 312
Size: 1205 1085 181 209 1924
Fragments arranged by size:
1924 1205 1085 209 181
BssSI C'ACGA_G
Cuts at: 328 2875 3233 328
Size: 2547 358 1699
Fragments arranged by size:
2547 1699 358
BstAPI GCAn_nnn'nTGC
Cuts at: 333 809 1981 333
Size: 476 1172 2956
Fragments arranged by size:
2956 1172 476
BstBI TT'CG_AA
Cuts at: 2965 2965
Size: 4604
BstYI r'GATC_y
Cuts at: 1 723 878 1166 1629 2096 2119 2454 2700
Size: 722 155 288 463 467 23 335 246
Cuts at: 2700 3712 3798 3810 4156 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 722 467 463 449 346 335 288
246 155 86 23 12
BtgI C'CryG_G
Cuts at: 107 659 107
Size: 552 4052
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2554 2735 2898 2554
Size: 181 163 4260
Fragments arranged by size:
4260 181 163
BtsI GCAGTG_nn'
Cuts at: 1847 1847
Size: 4604
DraI TTT'AAA
Cuts at: 1603 1603
Size: 4604
DraIII CAC_nnn'GTG
Cuts at: 1296 1296
Size: 4604
DrdI GACnn_nn'nnGTC
Cuts at: 2310 3168 2310
Size: 858 3746
EaeI y'GGCC_r
Cuts at: 389 592 662 1078 1577 2189 2363 2754 2781
Size: 203 70 416 499 612 174 391 27
Cuts at: 2781 4126 389
Size: 1345 867
Fragments arranged by size:
1345 867 612 499 416 391 203 174
70 27
EagI C'GGCC_G
Cuts at: 2189 2189
Size: 4604
EarI CTCTTCn'nnn_
Cuts at: 557 1121 2627 2837 557
Size: 564 1506 210 2324
Fragments arranged by size:
2324 1506 564 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2812 3122 3268 4414 2812
Size: 310 146 1146 3002
Fragments arranged by size:
3002 1146 310 146
EcoICRI GAG'CTC
Cuts at: 282 1422 1616 282
Size: 1140 194 3270
Fragments arranged by size:
3270 1140 194
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 581 724 895 937 1045 1519 1540 2429 2861
Size: 143 171 42 108 474 21 889 432
Cuts at: 2861 3608 3876 581
Size: 747 268 1309
Fragments arranged by size:
1309 889 747 474 432 268 171 143
108 42 21
EcoNI CCTnn'n_nnAGG
Cuts at: 1027 1276 1027
Size: 249 4355
EcoO109I rG'GnC_Cy
Cuts at: 1316 1529 1548 1743 1316
Size: 213 19 195 4177
Fragments arranged by size:
4177 213 195 19
EcoRI G'AATT_C
Cuts at: 841 1608 841
Size: 767 3837
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1150 2623 1150
Size: 1473 3131
FspI TGC'GCA
Cuts at: 2385 2385
Size: 4604
HaeII r_GCGC'y
Cuts at: 2038 2286 3308 4287 2038
Size: 248 1022 979 2355
Fragments arranged by size:
2355 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 184 216 2446 2476 2478 2508 184
Size: 32 2230 30 2 30 2280
Fragments arranged by size:
2280 2230 32 30 30 2
HincII GTy'rAC
Cuts at: 1643 1643
Size: 4604
KasI G'GCGC_C
Cuts at: 2034 2282 2034
Size: 248 4356
KpnI G_GTAC'C
Cuts at: 1624 1624
Size: 4604
MluI A'CGCG_T
Cuts at: 4037 4037
Size: 4604
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3275 3459 3275
Size: 184 4420
MscI TGG'CCA
Cuts at: 594 664 1080 2365 594
Size: 70 416 1285 2833
Fragments arranged by size:
2833 1285 416 70
MslI CAynn'nnrTG
Cuts at: 499 854 1396 2593 2720 499
Size: 355 542 1197 127 2383
Fragments arranged by size:
2383 1197 542 355 127
MspA1I CmG'CkG
Cuts at: 291 606 999 1560 1715 2029 2389 3402 3647
Size: 315 393 561 155 314 360 1013 245
Cuts at: 3647 291
Size: 1248
Fragments arranged by size:
1248 1013 561 393 360 315 314 245
155
NaeI GCC'GGC
Cuts at: 314 1519 2785 314
Size: 1205 1266 2133
Fragments arranged by size:
2133 1266 1205
NarI GG'CG_CC
Cuts at: 2035 2283 2035
Size: 248 4356
NcoI C'CATG_G
Cuts at: 107 107
Size: 4604
NgoMIV G'CCGG_C
Cuts at: 312 1517 2783 312
Size: 1205 1266 2133
Fragments arranged by size:
2133 1266 1205
NspI r_CATG'y
Cuts at: 1657 2157 3064 1657
Size: 500 907 3197
Fragments arranged by size:
3197 907 500
PasI CC'CwG_GG
Cuts at: 1533 1533
Size: 4604
PciI A'CATG_T
Cuts at: 3060 3060
Size: 4604
PfoI T'CCnGG_A
Cuts at: 701 1022 1169 701
Size: 321 147 4136
Fragments arranged by size:
4136 321 147
PmeI GTTT'AAAC
Cuts at: 1603 1603
Size: 4604
PmlI CAC'GTG
Cuts at: 1053 1053
Size: 4604
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3743 3775 3743
Size: 32 4572
PpuMI rG'GwC_Cy
Cuts at: 1316 1529 1316
Size: 213 4391
PstI C_TGCA'G
Cuts at: 605 1651 2336 605
Size: 1046 685 2873
Fragments arranged by size:
2873 1046 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4604
PvuII CAG'CTG
Cuts at: 606 999 2029 2389 606
Size: 393 1030 360 2821
Fragments arranged by size:
2821 1030 393 360
RsrII CG'GwC_CG
Cuts at: 2799 2799
Size: 4604
SacI G_AGCT'C
Cuts at: 284 1424 1618 284
Size: 1140 194 3270
Fragments arranged by size:
3270 1140 194
SalI G'TCGA_C
Cuts at: 1641 1641
Size: 4604
SanDI GG'GwC_CC
Cuts at: 1316 1529 1316
Size: 213 4391
SapI GCTCTTCn'nnn_
Cuts at: 2627 2837 2627
Size: 210 4394
SbfI CC_TGCA'GG
Cuts at: 1651 1651
Size: 4604
ScaI AGT'ACT
Cuts at: 4121 4121
Size: 4604
SfcI C'TryA_G
Cuts at: 33 601 1522 1647 2332 3325 3516 33
Size: 568 921 125 685 993 191 1121
Fragments arranged by size:
1121 993 921 685 568 191 125
SfiI GGCCn_nnn'nGGCC
Cuts at: 1546 1546
Size: 4604
SfoI GGC'GCC
Cuts at: 2036 2284 2036
Size: 248 4356
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4604
SmaI CCC'GGG
Cuts at: 159 460 730 1626 159
Size: 301 270 896 3137
Fragments arranged by size:
3137 896 301 270
SmlI C'TyrA_G
Cuts at: 397 580 745 1381 1759 2674 3166 3428 397
Size: 183 165 636 378 915 492 262 1573
Fragments arranged by size:
1573 915 636 492 378 262 183 165
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4604
SphI G_CATG'C
Cuts at: 1657 2157 1657
Size: 500 4104
StyI C'CwwG_G
Cuts at: 107 882 1334 1541 1738 2092 107
Size: 775 452 207 197 354 2619
Fragments arranged by size:
2619 775 452 354 207 197
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1108 2187 2320 2779 2988 1108
Size: 1079 133 459 209 2724
Fragments arranged by size:
2724 1079 459 209 133
TatI w'GTAC_w
Cuts at: 4119 4119
Size: 4604
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4121 4153 4121
Size: 32 4572
Tth111I GACn'n_nGTC
Cuts at: 2401 2401
Size: 4604
XbaI T'CTAG_A
Cuts at: 1635 1635
Size: 4604
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 672 2099 672
Size: 1427 3177
XmaI C'CCGG_G
Cuts at: 157 458 728 1624 157
Size: 301 270 896 3137
Fragments arranged by size:
3137 896 301 270
XmnI GAAnn'nnTTC
Cuts at: 517 1130 517
Size: 613 3991
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI AvrII BaeI BamHI
BanI BanII BbeI BbsI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgI
BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PasI
PciI PfoI PmeI PmlI PpiI PpuMI PstI PvuI
PvuII RsrII SacI SalI SanDI SapI SbfI ScaI
SfcI SfiI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AloI ApaI
AscI BbvCI BfrBI BmgBI BmtI BsiWI BsmI BsrGI
BssHII BstEII BstXI BstZ17I Bsu36I ClaI CspCI EcoRV
FseI FspAI HindIII HpaI MfeI NdeI NheI NotI
NruI NsiI PacI PflMI PshAI PsiI PspOMI PspXI
PsrI SacII SexAI SgrAI SnaBI SrfI SspI StuI
SwaI XhoI ZraI