Restriction map for
pF1KB5884
(Circular) (Six-base) MAPSORT of: pF1KB5884.seq Check: 215 from: 1 to: 4601
pF1KB5884 4601 bp
With 182 enzymes: SgfI *
March 2, 2010 14:26 ..
AccI GT'mk_AC
Cuts at: 1109 1639 1109
Size: 530 4071
Acc65I G'GTAC_C
Cuts at: 663 1617 663
Size: 954 3647
AclI AA'CG_TT
Cuts at: 4441 4441
Size: 4601
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1128 2426 2858 3605 3873 1128
Size: 1298 432 747 268 1856
Fragments arranged by size:
1856 1298 747 432 268
AflIII A'CryG_T
Cuts at: 1953 3057 4034 1953
Size: 1104 977 2520
Fragments arranged by size:
2520 1104 977
AgeI A'CCGG_T
Cuts at: 389 2989 389
Size: 2600 2001
AhdI GACnn_n'nnGTC
Cuts at: 1029 1410 1029
Size: 381 4220
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 904 904
Size: 4601
AlwNI CAG_nnn'CTG
Cuts at: 1906 3473 1906
Size: 1567 3034
ApaI G_GGCC'C
Cuts at: 245 1042 1142 1390 245
Size: 797 100 248 3456
Fragments arranged by size:
3456 797 248 100
ApaLI G'TGCA_C
Cuts at: 412 3371 412
Size: 2959 1642
ApoI r'AATT_y
Cuts at: 1605 3954 4210 1605
Size: 2349 256 1996
Fragments arranged by size:
2349 1996 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4601
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4601
AvaI C'yCGr_G
Cuts at: 238 560 952 1042 1160 1574 1621 238
Size: 322 392 90 118 414 47 3218
Fragments arranged by size:
3218 414 392 322 118 90 47
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1305 1338 1305
Size: 33 4568
BamHI G'GATC_C
Cuts at: 1626 1626
Size: 4601
BanI G'GyrC_C
Cuts at: 349 430 589 663 913 1128 1617 2031 2279
Size: 81 159 74 250 215 489 414 248
Cuts at: 2279 2314 349
Size: 35 2636
Fragments arranged by size:
2636 489 414 250 248 215 159 81
74 35
BanII G_rGCy'C
Cuts at: 118 245 337 874 1042 1142 1267 1308 1370
Size: 127 92 537 168 100 125 41 62
Cuts at: 1370 1390 1615 2645 118
Size: 20 225 1030 2074
Fragments arranged by size:
2074 1030 537 225 168 127 125 100
92 62 41 20
BbeI G_GCGC'C
Cuts at: 2035 2283 2035
Size: 248 4353
BbsI GAAGACnn'nnnn_
Cuts at: 429 429
Size: 4601
BbvCI CC'TCA_GC
Cuts at: 310 310
Size: 4601
BciVI GTATCCnnnnn_n'
Cuts at: 1333 2499 3260 4601 1333
Size: 1166 761 1341 1333
Fragments arranged by size:
1341 1333 1166 761
BclI T'GATC_A
Cuts at: 2121 2121
Size: 4601
BglI GCCn_nnn'nGGC
Cuts at: 240 878 1137 1394 240
Size: 638 259 257 3447
Fragments arranged by size:
3447 638 259 257
BglII A'GATC_T
Cuts at: 710 2116 4153 710
Size: 1406 2037 1158
Fragments arranged by size:
2037 1406 1158
BlpI GC'TnA_GC
Cuts at: 139 274 898 1713 139
Size: 135 624 815 3027
Fragments arranged by size:
3027 815 624 135
Bme1580I G_kGCm'C
Cuts at: 218 236 245 322 354 416 433 791 916
Size: 18 9 77 32 62 17 358 125
Cuts at: 916 1042 1142 1390 1538 2226 2319 3375 218
Size: 126 100 248 148 688 93 1056 1444
Fragments arranged by size:
1444 1056 688 358 248 148 126 125
100 93 77 62 32 18 17 9
BmrI ACTGGGnnnn_n'
Cuts at: 612 886 1285 1813 1894 2229 4373 612
Size: 274 399 528 81 335 2144 840
Fragments arranged by size:
2144 840 528 399 335 274 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 933 2968 933
Size: 2035 2566
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1039 1097 1546 1039
Size: 58 449 4094
Fragments arranged by size:
4094 449 58
Bpu10I CC'TnA_GC
Cuts at: 310 801 1330 310
Size: 491 529 3581
Fragments arranged by size:
3581 529 491
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1777 2656 3148 3446 1777
Size: 879 492 298 2932
Fragments arranged by size:
2932 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4305 4305
Size: 4601
BsaAI yAC'GTr
Cuts at: 360 1271 1954 360
Size: 911 683 3007
Fragments arranged by size:
3007 911 683
BsaBI GATnn'nnATC
Cuts at: 6 2115 4152 6
Size: 2109 2037 455
Fragments arranged by size:
2109 2037 455
BsaHI Gr'CG_yC
Cuts at: 536 1411 2032 2280 2982 4478 536
Size: 875 621 248 702 1496 659
Fragments arranged by size:
1496 875 702 659 621 248
BsaWI w'CCGG_w
Cuts at: 389 1790 2311 2989 3263 3410 3821 389
Size: 1401 521 678 274 147 411 1169
Fragments arranged by size:
1401 1169 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 376 406 770 800 1112 1142 376
Size: 30 364 30 312 30 3835
Fragments arranged by size:
3835 364 312 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 327 589 1057 1111 327
Size: 262 468 54 3817
Fragments arranged by size:
3817 468 262 54
BseYI C'CCAG_C
Cuts at: 363 459 993 1390 2026 2784 3361 3857 363
Size: 96 534 397 636 758 577 496 1107
Fragments arranged by size:
1107 758 636 577 534 496 397 96
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 340 502 616 658 910 340
Size: 162 114 42 252 4031
Fragments arranged by size:
4031 252 162 114 42
BsiEI CG_ry'CG
Cuts at: 104 762 2143 2189 3397 104
Size: 658 1381 46 1208 1308
Fragments arranged by size:
1381 1308 1208 658 46
BsiHKAI G_wGCw'C
Cuts at: 118 179 389 416 1615 2393 2583 3375 118
Size: 61 210 27 1199 778 190 792 1344
Fragments arranged by size:
1344 1199 792 778 210 190 61 27
Bsp1286I G_dGCh'C
Cuts at: 118 179 218 236 245 322 337 354 389
Size: 61 39 18 9 77 15 17 35
Cuts at: 389 416 433 791 874 916 1042 1142 1267
Size: 27 17 358 83 42 126 100 125
Cuts at: 1267 1308 1370 1390 1538 1615 2226 2319 2393
Size: 41 62 20 148 77 611 93 74
Cuts at: 2393 2583 2645 3375 118
Size: 190 62 730 1344
Fragments arranged by size:
1344 730 611 358 190 148 126 125
100 93 83 77 77 74 62 62
61 42 41 39 35 27 20 18
17 17 15 9
BspEI T'CCGG_A
Cuts at: 3821 3821
Size: 4601
BspHI T'CATG_A
Cuts at: 3777 3777
Size: 4601
BspMI ACCTGCnnnn'nnnn_
Cuts at: 803 1651 1865 2167 2548 803
Size: 848 214 302 381 2856
Fragments arranged by size:
2856 848 381 302 214
BsrBI CCG'CTC
Cuts at: 50 186 1466 2893 2947 4431 4596 50
Size: 136 1280 1427 54 1484 165 55
Fragments arranged by size:
1484 1427 1280 165 136 55 54
BsrDI GCAATG_nn'
Cuts at: 867 2513 867
Size: 1646 2955
BsrFI r'CCGG_y
Cuts at: 389 538 841 1422 2599 2780 2989 389
Size: 149 303 581 1177 181 209 2001
Fragments arranged by size:
2001 1177 581 303 209 181 149
BsrGI T'GTAC_A
Cuts at: 1501 1501
Size: 4601
BssHII G'CGCG_C
Cuts at: 508 508
Size: 4601
BssSI C'ACGA_G
Cuts at: 546 784 2872 3230 546
Size: 238 2088 358 1917
Fragments arranged by size:
2088 1917 358 238
BstAPI GCAn_nnn'nTGC
Cuts at: 713 1978 713
Size: 1265 3336
BstBI TT'CG_AA
Cuts at: 2962 2962
Size: 4601
BstXI CCAn_nnnn'nTGG
Cuts at: 252 1132 252
Size: 880 3721
BstYI r'GATC_y
Cuts at: 1 710 1626 2093 2116 2451 2697 3709 3795
Size: 709 916 467 23 335 246 1012 86
Cuts at: 3795 3807 4153 1
Size: 12 346 449
Fragments arranged by size:
1012 916 709 467 449 346 335 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 473 648 1430 473
Size: 175 782 3644
Fragments arranged by size:
3644 782 175
BtgI C'CryG_G
Cuts at: 491 630 756 1015 1116 491
Size: 139 126 259 101 3976
Fragments arranged by size:
3976 259 139 126 101
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 435 764 2551 2732 2895 435
Size: 329 1787 181 163 2141
Fragments arranged by size:
2141 1787 329 181 163
BtsI GCAGTG_nn'
Cuts at: 1844 1844
Size: 4601
DraI TTT'AAA
Cuts at: 1600 1600
Size: 4601
DraIII CAC_nnn'GTG
Cuts at: 636 1293 636
Size: 657 3944
DrdI GACnn_nn'nnGTC
Cuts at: 2307 3165 2307
Size: 858 3743
EaeI y'GGCC_r
Cuts at: 326 524 627 759 2186 2360 2751 2778 4123
Size: 198 103 132 1427 174 391 27 1345
Cuts at: 4123 326
Size: 804
Fragments arranged by size:
1427 1345 804 391 198 174 132 103
27
EagI C'GGCC_G
Cuts at: 759 2186 759
Size: 1427 3174
EarI CTCTTCn'nnn_
Cuts at: 184 448 2624 2834 184
Size: 264 2176 210 1951
Fragments arranged by size:
2176 1951 264 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 397 1315 2809 3119 3265 4411 397
Size: 918 1494 310 146 1146 587
Fragments arranged by size:
1494 1146 918 587 310 146
EcoICRI GAG'CTC
Cuts at: 116 1613 116
Size: 1497 3104
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1039 1097 1128 1546 2426 2858 3605 3873 1039
Size: 58 31 418 880 432 747 268 1767
Fragments arranged by size:
1767 880 747 432 418 268 58 31
EcoNI CCTnn'n_nnAGG
Cuts at: 129 571 1516 129
Size: 442 945 3214
Fragments arranged by size:
3214 945 442
EcoO109I rG'GnC_Cy
Cuts at: 150 564 622 1039 1139 1217 1386 1387 1437
Size: 414 58 417 100 78 169 1 50
Cuts at: 1437 1475 1521 1740 150
Size: 38 46 219 3011
Fragments arranged by size:
3011 417 414 219 169 100 78 58
50 46 38 1
EcoRI G'AATT_C
Cuts at: 1605 1605
Size: 4601
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2620 2620
Size: 4601
FspI TGC'GCA
Cuts at: 2382 2382
Size: 4601
HaeII r_GCGC'y
Cuts at: 560 774 898 2035 2283 3305 4284 560
Size: 214 124 1137 248 1022 979 877
Fragments arranged by size:
1137 1022 979 877 248 214 124
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 376 408 711 743 1015 1047 1201 1233 1396
Size: 32 303 32 272 32 154 32 163
Cuts at: 1396 1428 2443 2473 2475 2505 376
Size: 32 1015 30 2 30 2472
Fragments arranged by size:
2472 1015 303 272 163 154 32 32
32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1640 1640
Size: 4601
KasI G'GCGC_C
Cuts at: 2031 2279 2031
Size: 248 4353
KpnI G_GTAC'C
Cuts at: 667 1621 667
Size: 954 3647
MluI A'CGCG_T
Cuts at: 4034 4034
Size: 4601
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1427 3272 3456 1427
Size: 1845 184 2572
Fragments arranged by size:
2572 1845 184
MscI TGG'CCA
Cuts at: 526 2362 526
Size: 1836 2765
MslI CAynn'nnrTG
Cuts at: 454 2590 2717 454
Size: 2136 127 2338
Fragments arranged by size:
2338 2136 127
MspA1I CmG'CkG
Cuts at: 270 642 816 1344 1358 1394 1712 2026 2386
Size: 372 174 528 14 36 318 314 360
Cuts at: 2386 3399 3644 270
Size: 1013 245 1227
Fragments arranged by size:
1227 1013 528 372 360 318 314 245
174 36 14
NaeI GCC'GGC
Cuts at: 540 843 1424 2782 540
Size: 303 581 1358 2359
Fragments arranged by size:
2359 1358 581 303
NarI GG'CG_CC
Cuts at: 2032 2280 2032
Size: 248 4353
NgoMIV G'CCGG_C
Cuts at: 538 841 1422 2780 538
Size: 303 581 1358 2359
Fragments arranged by size:
2359 1358 581 303
NspI r_CATG'y
Cuts at: 1654 2154 3061 1654
Size: 500 907 3194
Fragments arranged by size:
3194 907 500
PasI CC'CwG_GG
Cuts at: 1370 1370
Size: 4601
PciI A'CATG_T
Cuts at: 3057 3057
Size: 4601
PflMI CCAn_nnn'nTGG
Cuts at: 303 1293 303
Size: 990 3611
PfoI T'CCnGG_A
Cuts at: 1074 1219 1523 1074
Size: 145 304 4152
Fragments arranged by size:
4152 304 145
PmeI GTTT'AAAC
Cuts at: 1600 1600
Size: 4601
PmlI CAC'GTG
Cuts at: 360 1271 360
Size: 911 3690
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3740 3772 3740
Size: 32 4569
PpuMI rG'GwC_Cy
Cuts at: 150 564 622 1217 1475 1521 150
Size: 414 58 595 258 46 3230
Fragments arranged by size:
3230 595 414 258 58 46
PspOMI G'GGCC_C
Cuts at: 241 1038 1138 1386 241
Size: 797 100 248 3456
Fragments arranged by size:
3456 797 248 100
PstI C_TGCA'G
Cuts at: 123 1157 1648 2333 123
Size: 1034 491 685 2391
Fragments arranged by size:
2391 1034 685 491
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4601
PvuII CAG'CTG
Cuts at: 642 816 1358 2026 2386 642
Size: 174 542 668 360 2857
Fragments arranged by size:
2857 668 542 360 174
RsrII CG'GwC_CG
Cuts at: 2796 2796
Size: 4601
SacI G_AGCT'C
Cuts at: 118 1615 118
Size: 1497 3104
SalI G'TCGA_C
Cuts at: 1638 1638
Size: 4601
SanDI GG'GwC_CC
Cuts at: 150 622 150
Size: 472 4129
SapI GCTCTTCn'nnn_
Cuts at: 184 2624 2834 184
Size: 2440 210 1951
Fragments arranged by size:
2440 1951 210
SbfI CC_TGCA'GG
Cuts at: 1648 1648
Size: 4601
ScaI AGT'ACT
Cuts at: 4118 4118
Size: 4601
SfcI C'TryA_G
Cuts at: 33 119 744 1106 1153 1644 2329 3322 3513
Size: 86 625 362 47 491 685 993 191
Cuts at: 3513 33
Size: 1121
Fragments arranged by size:
1121 993 685 625 491 362 191 86
47
SfoI GGC'GCC
Cuts at: 2033 2281 2033
Size: 248 4353
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4601
SmaI CCC'GGG
Cuts at: 240 562 1044 1623 240
Size: 322 482 579 3218
Fragments arranged by size:
3218 579 482 322
SmlI C'TyrA_G
Cuts at: 1756 2671 3163 3425 1756
Size: 915 492 262 2932
Fragments arranged by size:
2932 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4601
SphI G_CATG'C
Cuts at: 1654 2154 1654
Size: 500 4101
StuI AGG'CCT
Cuts at: 782 1194 782
Size: 412 4189
StyI C'CwwG_G
Cuts at: 130 1560 1735 2089 130
Size: 1430 175 354 2642
Fragments arranged by size:
2642 1430 354 175
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1060 1304 1362 2184 2317 2776 2985 1060
Size: 244 58 822 133 459 209 2676
Fragments arranged by size:
2676 822 459 244 209 133 58
TatI w'GTAC_w
Cuts at: 1280 1501 4116 1280
Size: 221 2615 1765
Fragments arranged by size:
2615 1765 221
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 770 802 1112 1144 4118 4150 770
Size: 32 310 32 2974 32 1221
Fragments arranged by size:
2974 1221 310 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2398 2398
Size: 4601
XbaI T'CTAG_A
Cuts at: 1632 1632
Size: 4601
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 615 1132 2096 615
Size: 517 964 3120
Fragments arranged by size:
3120 964 517
XmaI C'CCGG_G
Cuts at: 238 560 1042 1621 238
Size: 322 482 579 3218
Fragments arranged by size:
3218 579 482 322
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AlfI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI
BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI
BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NgoMIV NspI PasI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PspOMI PstI PvuI PvuII RsrII SacI
SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AleI AloI AscI AvrII
BcgI BfrBI BmgBI BmtI BsiWI BsmI BsmBI BstEII
BstZ17I ClaI CspCI EcoRV FseI FspAI HindIII HpaI
MfeI NcoI NdeI NheI NotI NruI NsiI PacI
PshAI PsiI PspXI PsrI SacII SexAI SfiI SgrAI
SnaBI SrfI SspI SwaI XhoI XmnI ZraI