Restriction map for
pF1KB5883
(Circular) (Six-base) MAPSORT of: pF1KB5883.seq Check: 2093 from: 1 to: 4514
pF1KB5883 4514 bp
With 182 enzymes: SgfI *
October 7, 2009 14:18 ..
AccI GT'mk_AC
Cuts at: 1552 1552
Size: 4514
Acc65I G'GTAC_C
Cuts at: 1530 1530
Size: 4514
AclI AA'CG_TT
Cuts at: 4354 4354
Size: 4514
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 190 2339 2771 3518 3786 190
Size: 2149 432 747 268 918
Fragments arranged by size:
2149 918 747 432 268
AflIII A'CryG_T
Cuts at: 1866 2970 3947 1866
Size: 1104 977 2433
Fragments arranged by size:
2433 1104 977
AgeI A'CCGG_T
Cuts at: 2902 2902
Size: 4514
AhdI GACnn_n'nnGTC
Cuts at: 445 445
Size: 4514
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1486 1518 1486
Size: 32 4482
AlwNI CAG_nnn'CTG
Cuts at: 936 1182 1819 3386 936
Size: 246 637 1567 2064
Fragments arranged by size:
2064 1567 637 246
ApaI G_GGCC'C
Cuts at: 128 411 828 1171 128
Size: 283 417 343 3471
Fragments arranged by size:
3471 417 343 283
ApaLI G'TGCA_C
Cuts at: 3284 3284
Size: 4514
ApoI r'AATT_y
Cuts at: 460 1500 1518 3867 4123 460
Size: 1040 18 2349 256 851
Fragments arranged by size:
2349 1040 851 256 18
AseI AT'TA_AT
Cuts at: 20 20
Size: 4514
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4514
AvaI C'yCGr_G
Cuts at: 427 1534 427
Size: 1107 3407
AvrII C'CTAG_G
Cuts at: 726 726
Size: 4514
BamHI G'GATC_C
Cuts at: 1539 1539
Size: 4514
BanI G'GyrC_C
Cuts at: 1530 1944 2192 2227 1530
Size: 414 248 35 3817
Fragments arranged by size:
3817 414 248 35
BanII G_rGCy'C
Cuts at: 128 411 828 1040 1171 1528 2558 128
Size: 283 417 212 131 357 1030 2084
Fragments arranged by size:
2084 1030 417 357 283 212 131
BbeI G_GCGC'C
Cuts at: 1948 2196 1948
Size: 248 4266
BciVI GTATCCnnnnn_n'
Cuts at: 550 2412 3173 4514 550
Size: 1862 761 1341 550
Fragments arranged by size:
1862 1341 761 550
BclI T'GATC_A
Cuts at: 2034 2034
Size: 4514
BglI GCCn_nnn'nGGC
Cuts at: 333 956 333
Size: 623 3891
BglII A'GATC_T
Cuts at: 2029 4066 2029
Size: 2037 2477
BlpI GC'TnA_GC
Cuts at: 1626 1626
Size: 4514
Bme1580I G_kGCm'C
Cuts at: 128 260 411 725 828 904 1171 2139 2232
Size: 132 151 314 103 76 267 968 93
Cuts at: 2232 3288 128
Size: 1056 1354
Fragments arranged by size:
1354 1056 968 314 267 151 132 103
93 76
BmrI ACTGGGnnnn_n'
Cuts at: 831 866 1726 1807 2142 4286 831
Size: 35 860 81 335 2144 1059
Fragments arranged by size:
2144 1059 860 335 81 35
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1419 2881 1419
Size: 1462 3052
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 368 454 1180 368
Size: 86 726 3702
Fragments arranged by size:
3702 726 86
Bpu10I CC'TnA_GC
Cuts at: 1289 1289
Size: 4514
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1243 1690 2569 3061 3359 1243
Size: 447 879 492 298 2398
Fragments arranged by size:
2398 879 492 447 298
BsaI GGTCTCn'nnnn_
Cuts at: 561 1407 4218 561
Size: 846 2811 857
Fragments arranged by size:
2811 857 846
BsaAI yAC'GTr
Cuts at: 1867 1867
Size: 4514
BsaBI GATnn'nnATC
Cuts at: 6 2028 4065 6
Size: 2022 2037 455
Fragments arranged by size:
2037 2022 455
BsaHI Gr'CG_yC
Cuts at: 1945 2193 2895 4391 1945
Size: 248 702 1496 2068
Fragments arranged by size:
2068 1496 702 248
BsaWI w'CCGG_w
Cuts at: 882 1703 2224 2902 3176 3323 3734 882
Size: 821 521 678 274 147 411 1662
Fragments arranged by size:
1662 821 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 461 1090 1107 1116 461
Size: 629 17 9 3859
Fragments arranged by size:
3859 629 17 9
BseYI C'CCAG_C
Cuts at: 182 312 336 500 1939 2697 3274 3770 182
Size: 130 24 164 1439 758 577 496 926
Fragments arranged by size:
1439 926 758 577 496 164 130 24
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 766 1217 766
Size: 451 4063
BsiEI CG_ry'CG
Cuts at: 104 2056 2102 3310 104
Size: 1952 46 1208 1308
Fragments arranged by size:
1952 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 806 1368 1528 2306 2496 3288 806
Size: 562 160 778 190 792 2032
Fragments arranged by size:
2032 792 778 562 190 160
Bsp1286I G_dGCh'C
Cuts at: 128 260 411 725 806 828 904 1040 1171
Size: 132 151 314 81 22 76 136 131
Cuts at: 1171 1368 1528 2139 2232 2306 2496 2558 3288
Size: 197 160 611 93 74 190 62 730
Cuts at: 3288 128
Size: 1354
Fragments arranged by size:
1354 730 611 314 197 190 160 151
136 132 131 93 81 76 74 62
22
BspEI T'CCGG_A
Cuts at: 882 3734 882
Size: 2852 1662
BspHI T'CATG_A
Cuts at: 3690 3690
Size: 4514
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1564 1778 2080 2461 1564
Size: 214 302 381 3617
Fragments arranged by size:
3617 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2806 2860 4344 4509 50
Size: 2756 54 1484 165 55
Fragments arranged by size:
2756 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1094 2426 1094
Size: 1332 3182
BsrFI r'CCGG_y
Cuts at: 2512 2693 2902 2512
Size: 181 209 4124
Fragments arranged by size:
4124 209 181
BssSI C'ACGA_G
Cuts at: 2785 3143 2785
Size: 358 4156
BstAPI GCAn_nnn'nTGC
Cuts at: 277 1891 277
Size: 1614 2900
BstBI TT'CG_AA
Cuts at: 2875 2875
Size: 4514
BstXI CCAn_nnnn'nTGG
Cuts at: 1398 1398
Size: 4514
BstYI r'GATC_y
Cuts at: 1 654 1209 1539 2006 2029 2364 2610 3622
Size: 653 555 330 467 23 335 246 1012
Cuts at: 3622 3708 3720 4066 1
Size: 86 12 346 449
Fragments arranged by size:
1012 653 555 467 449 346 335 330
246 86 23 12
BtgI C'CryG_G
Cuts at: 107 119 107
Size: 12 4502
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2464 2645 2808 2464
Size: 181 163 4170
Fragments arranged by size:
4170 181 163
BtsI GCAGTG_nn'
Cuts at: 1757 1757
Size: 4514
DraI TTT'AAA
Cuts at: 1513 1513
Size: 4514
DrdI GACnn_nn'nnGTC
Cuts at: 2220 3078 2220
Size: 858 3656
EaeI y'GGCC_r
Cuts at: 158 1013 2099 2273 2664 2691 4036 158
Size: 855 1086 174 391 27 1345 636
Fragments arranged by size:
1345 1086 855 636 391 174 27
EagI C'GGCC_G
Cuts at: 2099 2099
Size: 4514
EarI CTCTTCn'nnn_
Cuts at: 288 336 518 1270 2537 2747 288
Size: 48 182 752 1267 210 2055
Fragments arranged by size:
2055 1267 752 210 182 48
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2722 3032 3178 4324 2722
Size: 310 146 1146 2912
Fragments arranged by size:
2912 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1526 1526
Size: 4514
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 190 368 454 1180 2339 2771 3518 3786 190
Size: 178 86 726 1159 432 747 268 918
Fragments arranged by size:
1159 918 747 726 432 268 178 86
EcoO109I rG'GnC_Cy
Cuts at: 124 174 207 325 369 447 812 825 998
Size: 50 33 118 44 78 365 13 173
Cuts at: 998 1066 1168 1653 124
Size: 68 102 485 2985
Fragments arranged by size:
2985 485 365 173 118 102 78 68
50 44 33 13
EcoRI G'AATT_C
Cuts at: 460 1500 1518 460
Size: 1040 18 3456
Fragments arranged by size:
3456 1040 18
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2533 2533
Size: 4514
FspI TGC'GCA
Cuts at: 2295 2295
Size: 4514
HaeII r_GCGC'y
Cuts at: 1948 2196 3218 4197 1948
Size: 248 1022 979 2265
Fragments arranged by size:
2265 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 431 463 639 671 1155 1187 2356 2386 2388
Size: 32 176 32 484 32 1169 30 2
Cuts at: 2388 2418 431
Size: 30 2527
Fragments arranged by size:
2527 1169 484 176 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1203 1553 1203
Size: 350 4164
KasI G'GCGC_C
Cuts at: 1944 2192 1944
Size: 248 4266
KpnI G_GTAC'C
Cuts at: 1534 1534
Size: 4514
MluI A'CGCG_T
Cuts at: 3947 3947
Size: 4514
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1013 1349 3185 3369 1013
Size: 336 1836 184 2158
Fragments arranged by size:
2158 1836 336 184
MscI TGG'CCA
Cuts at: 160 1015 2275 160
Size: 855 1260 2399
Fragments arranged by size:
2399 1260 855
MslI CAynn'nnrTG
Cuts at: 897 917 2503 2630 897
Size: 20 1586 127 2781
Fragments arranged by size:
2781 1586 127 20
MspA1I CmG'CkG
Cuts at: 1625 1939 2299 3312 3557 1625
Size: 314 360 1013 245 2582
Fragments arranged by size:
2582 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2695 2695
Size: 4514
NarI GG'CG_CC
Cuts at: 1945 2193 1945
Size: 248 4266
NcoI C'CATG_G
Cuts at: 107 107
Size: 4514
NgoMIV G'CCGG_C
Cuts at: 2693 2693
Size: 4514
NspI r_CATG'y
Cuts at: 280 1567 2067 2974 280
Size: 1287 500 907 1820
Fragments arranged by size:
1820 1287 907 500
PasI CC'CwG_GG
Cuts at: 1300 1300
Size: 4514
PciI A'CATG_T
Cuts at: 2970 2970
Size: 4514
PflMI CCAn_nnn'nTGG
Cuts at: 365 856 1300 365
Size: 491 444 3579
Fragments arranged by size:
3579 491 444
PmeI GTTT'AAAC
Cuts at: 1513 1513
Size: 4514
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3653 3685 3653
Size: 32 4482
PpuMI rG'GwC_Cy
Cuts at: 207 369 447 998 1066 207
Size: 162 78 551 68 3655
Fragments arranged by size:
3655 551 162 78 68
PspOMI G'GGCC_C
Cuts at: 124 407 824 1167 124
Size: 283 417 343 3471
Fragments arranged by size:
3471 417 343 283
PstI C_TGCA'G
Cuts at: 154 1561 2246 154
Size: 1407 685 2422
Fragments arranged by size:
2422 1407 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4514
PvuII CAG'CTG
Cuts at: 1939 2299 1939
Size: 360 4154
RsrII CG'GwC_CG
Cuts at: 2709 2709
Size: 4514
SacI G_AGCT'C
Cuts at: 1528 1528
Size: 4514
SalI G'TCGA_C
Cuts at: 1551 1551
Size: 4514
SapI GCTCTTCn'nnn_
Cuts at: 336 2537 2747 336
Size: 2201 210 2103
Fragments arranged by size:
2201 2103 210
SbfI CC_TGCA'GG
Cuts at: 1561 1561
Size: 4514
ScaI AGT'ACT
Cuts at: 4031 4031
Size: 4514
SfcI C'TryA_G
Cuts at: 33 150 551 1373 1557 2242 3235 3426 33
Size: 117 401 822 184 685 993 191 1121
Fragments arranged by size:
1121 993 822 685 401 191 184 117
SfoI GGC'GCC
Cuts at: 1946 2194 1946
Size: 248 4266
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4514
SmaI CCC'GGG
Cuts at: 1536 1536
Size: 4514
SmlI C'TyrA_G
Cuts at: 1222 1669 2584 3076 3338 1222
Size: 447 915 492 262 2398
Fragments arranged by size:
2398 915 492 447 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4514
SphI G_CATG'C
Cuts at: 280 1567 2067 280
Size: 1287 500 2727
Fragments arranged by size:
2727 1287 500
StyI C'CwwG_G
Cuts at: 107 202 726 1391 1648 2002 107
Size: 95 524 665 257 354 2619
Fragments arranged by size:
2619 665 524 354 257 95
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2097 2230 2689 2898 2097
Size: 133 459 209 3713
Fragments arranged by size:
3713 459 209 133
TatI w'GTAC_w
Cuts at: 4029 4029
Size: 4514
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4031 4063 4031
Size: 32 4482
Tth111I GACn'n_nGTC
Cuts at: 2311 2311
Size: 4514
XbaI T'CTAG_A
Cuts at: 1545 1545
Size: 4514
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 429 613 2009 429
Size: 184 1396 2934
Fragments arranged by size:
2934 1396 184
XmaI C'CCGG_G
Cuts at: 1534 1534
Size: 4514
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AloI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII
BamHI BanI BanII BbeI BciVI BclI BglI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI
BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PasI PciI PflMI PmeI PpiI
PpuMI PspOMI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AleI AlfI AscI BaeI
BbsI BbvCI BcgI BfrBI BmgBI BmtI BsaXI BsiWI
BsmI BsmBI BsrGI BssHII BstEII BstZ17I Bsu36I ClaI
CspCI DraIII EcoNI EcoRV FseI FspAI HindIII HpaI
MfeI NdeI NheI NotI NruI NsiI PacI PfoI
PmlI PshAI PsiI PspXI PsrI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI
XmnI ZraI