Restriction map for
pF1KB5867
(Circular) (Six-base) MAPSORT of: pF1KB5867.seq Check: 9651 from: 1 to: 4499
pF1KB5867 4499 bp
With 182 enzymes: SgfI *
October 7, 2009 14:18 ..
AccI GT'mk_AC
Cuts at: 912 1537 912
Size: 625 3874
Acc65I G'GTAC_C
Cuts at: 1515 1515
Size: 4499
AclI AA'CG_TT
Cuts at: 4339 4339
Size: 4499
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 605 1391 2324 2756 3503 3771 605
Size: 786 933 432 747 268 1333
Fragments arranged by size:
1333 933 786 747 432 268
AflIII A'CryG_T
Cuts at: 1851 2955 3932 1851
Size: 1104 977 2418
Fragments arranged by size:
2418 1104 977
AgeI A'CCGG_T
Cuts at: 2887 2887
Size: 4499
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1213 1213
Size: 4499
AlwNI CAG_nnn'CTG
Cuts at: 1804 3371 1804
Size: 1567 2932
ApaLI G'TGCA_C
Cuts at: 3269 3269
Size: 4499
ApoI r'AATT_y
Cuts at: 622 1333 1503 3852 4108 622
Size: 711 170 2349 256 1013
Fragments arranged by size:
2349 1013 711 256 170
AseI AT'TA_AT
Cuts at: 20 20
Size: 4499
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4499
AvaI C'yCGr_G
Cuts at: 1519 1519
Size: 4499
BamHI G'GATC_C
Cuts at: 1524 1524
Size: 4499
BanI G'GyrC_C
Cuts at: 1366 1515 1929 2177 2212 1366
Size: 149 414 248 35 3653
Fragments arranged by size:
3653 414 248 149 35
BanII G_rGCy'C
Cuts at: 1513 2543 1513
Size: 1030 3469
BbeI G_GCGC'C
Cuts at: 1370 1933 2181 1370
Size: 563 248 3688
Fragments arranged by size:
3688 563 248
BbsI GAAGACnn'nnnn_
Cuts at: 205 665 947 1052 205
Size: 460 282 105 3652
Fragments arranged by size:
3652 460 282 105
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1204 1238 1204
Size: 34 4465
BciVI GTATCCnnnnn_n'
Cuts at: 2397 3158 4499 2397
Size: 761 1341 2397
Fragments arranged by size:
2397 1341 761
BclI T'GATC_A
Cuts at: 2019 2019
Size: 4499
BfrBI ATG'CAT
Cuts at: 736 736
Size: 4499
BglII A'GATC_T
Cuts at: 768 2014 4051 768
Size: 1246 2037 1216
Fragments arranged by size:
2037 1246 1216
BlpI GC'TnA_GC
Cuts at: 1611 1611
Size: 4499
Bme1580I G_kGCm'C
Cuts at: 537 2124 2217 3273 537
Size: 1587 93 1056 1763
Fragments arranged by size:
1763 1587 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1711 1792 2127 4271 1711
Size: 81 335 2144 1939
Fragments arranged by size:
2144 1939 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2866 2866
Size: 4499
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 228 228
Size: 4499
Bpu10I CC'TnA_GC
Cuts at: 131 287 131
Size: 156 4343
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1675 2554 3046 3344 1675
Size: 879 492 298 2830
Fragments arranged by size:
2830 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 857 4203 857
Size: 3346 1153
BsaAI yAC'GTr
Cuts at: 1458 1852 1458
Size: 394 4105
BsaBI GATnn'nnATC
Cuts at: 6 812 2013 4050 6
Size: 806 1201 2037 455
Fragments arranged by size:
2037 1201 806 455
BsaHI Gr'CG_yC
Cuts at: 1367 1930 2178 2880 4376 1367
Size: 563 248 702 1496 1490
Fragments arranged by size:
1496 1490 702 563 248
BsaWI w'CCGG_w
Cuts at: 1688 2209 2887 3161 3308 3719 1688
Size: 521 678 274 147 411 2468
Fragments arranged by size:
2468 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 809 809
Size: 4499
BseYI C'CCAG_C
Cuts at: 750 1924 2682 3259 3755 750
Size: 1174 758 577 496 1494
Fragments arranged by size:
1494 1174 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 2041 2087 3295 104
Size: 1937 46 1208 1308
Fragments arranged by size:
1937 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1033 1513 2291 2481 3273 1033
Size: 480 778 190 792 2259
Fragments arranged by size:
2259 792 778 480 190
BsmI GAATG_Cn'
Cuts at: 1241 1241
Size: 4499
Bsp1286I G_dGCh'C
Cuts at: 537 1033 1513 2124 2217 2291 2481 2543 3273
Size: 496 480 611 93 74 190 62 730
Cuts at: 3273 537
Size: 1763
Fragments arranged by size:
1763 730 611 496 480 190 93 74
62
BspEI T'CCGG_A
Cuts at: 3719 3719
Size: 4499
BspHI T'CATG_A
Cuts at: 3675 3675
Size: 4499
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1395 1549 1763 2065 2446 1395
Size: 154 214 302 381 3448
Fragments arranged by size:
3448 381 302 214 154
BsrBI CCG'CTC
Cuts at: 50 2791 2845 4329 4494 50
Size: 2741 54 1484 165 55
Fragments arranged by size:
2741 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2411 2411
Size: 4499
BsrFI r'CCGG_y
Cuts at: 2497 2678 2887 2497
Size: 181 209 4109
Fragments arranged by size:
4109 209 181
BssSI C'ACGA_G
Cuts at: 2770 3128 2770
Size: 358 4141
BstAPI GCAn_nnn'nTGC
Cuts at: 124 1876 124
Size: 1752 2747
BstBI TT'CG_AA
Cuts at: 2860 2860
Size: 4499
BstYI r'GATC_y
Cuts at: 1 768 807 1219 1443 1524 1991 2014 2349
Size: 767 39 412 224 81 467 23 335
Cuts at: 2349 2595 3607 3693 3705 4051 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1012 767 467 449 412 346 335 246
224 86 81 39 23 12
BstZ17I GTA'TAC
Cuts at: 913 913
Size: 4499
Bsu36I CC'TnA_GG
Cuts at: 950 950
Size: 4499
BtgI C'CryG_G
Cuts at: 182 260 711 182
Size: 78 451 3970
Fragments arranged by size:
3970 451 78
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2449 2630 2793 2449
Size: 181 163 4155
Fragments arranged by size:
4155 181 163
BtsI GCAGTG_nn'
Cuts at: 225 342 1195 1742 225
Size: 117 853 547 2982
Fragments arranged by size:
2982 853 547 117
DraI TTT'AAA
Cuts at: 228 242 1498 228
Size: 14 1256 3229
Fragments arranged by size:
3229 1256 14
DrdI GACnn_nn'nnGTC
Cuts at: 2205 3063 2205
Size: 858 3641
EaeI y'GGCC_r
Cuts at: 174 339 1452 2084 2258 2649 2676 4021 174
Size: 165 1113 632 174 391 27 1345 652
Fragments arranged by size:
1345 1113 652 632 391 174 165 27
EagI C'GGCC_G
Cuts at: 2084 2084
Size: 4499
EarI CTCTTCn'nnn_
Cuts at: 285 395 422 569 2522 2732 285
Size: 110 27 147 1953 210 2052
Fragments arranged by size:
2052 1953 210 147 110 27
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2707 3017 3163 4309 2707
Size: 310 146 1146 2897
Fragments arranged by size:
2897 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1511 1511
Size: 4499
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 228 605 1391 2324 2756 3503 3771 228
Size: 377 786 933 432 747 268 956
Fragments arranged by size:
956 933 786 747 432 377 268
EcoO109I rG'GnC_Cy
Cuts at: 1638 1638
Size: 4499
EcoRI G'AATT_C
Cuts at: 622 1503 622
Size: 881 3618
EcoRV GAT'ATC
Cuts at: 899 899
Size: 4499
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 396 1094 2518 396
Size: 698 1424 2377
Fragments arranged by size:
2377 1424 698
FspI TGC'GCA
Cuts at: 2280 2280
Size: 4499
HaeII r_GCGC'y
Cuts at: 297 1370 1933 2181 3203 4182 297
Size: 1073 563 248 1022 979 614
Fragments arranged by size:
1073 1022 979 614 563 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2341 2371 2373 2403 2341
Size: 30 2 30 4437
Fragments arranged by size:
4437 30 30 2
HincII GTy'rAC
Cuts at: 1538 1538
Size: 4499
HindIII A'AGCT_T
Cuts at: 705 705
Size: 4499
KasI G'GCGC_C
Cuts at: 1366 1929 2177 1366
Size: 563 248 3688
Fragments arranged by size:
3688 563 248
KpnI G_GTAC'C
Cuts at: 1519 1519
Size: 4499
MluI A'CGCG_T
Cuts at: 3932 3932
Size: 4499
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 926 3170 3354 926
Size: 2244 184 2071
Fragments arranged by size:
2244 2071 184
MscI TGG'CCA
Cuts at: 341 1454 2260 341
Size: 1113 806 2580
Fragments arranged by size:
2580 1113 806
MslI CAynn'nnrTG
Cuts at: 2488 2615 2488
Size: 127 4372
MspA1I CmG'CkG
Cuts at: 754 868 1610 1924 2284 3297 3542 754
Size: 114 742 314 360 1013 245 1711
Fragments arranged by size:
1711 1013 742 360 314 245 114
NaeI GCC'GGC
Cuts at: 2680 2680
Size: 4499
NarI GG'CG_CC
Cuts at: 1367 1930 2178 1367
Size: 563 248 3688
Fragments arranged by size:
3688 563 248
NcoI C'CATG_G
Cuts at: 182 260 182
Size: 78 4421
NgoMIV G'CCGG_C
Cuts at: 2678 2678
Size: 4499
NsiI A_TGCA'T
Cuts at: 738 738
Size: 4499
NspI r_CATG'y
Cuts at: 316 1552 2052 2959 316
Size: 1236 500 907 1856
Fragments arranged by size:
1856 1236 907 500
PciI A'CATG_T
Cuts at: 2955 2955
Size: 4499
PmeI GTTT'AAAC
Cuts at: 1498 1498
Size: 4499
PmlI CAC'GTG
Cuts at: 1458 1458
Size: 4499
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3638 3670 3638
Size: 32 4467
PstI C_TGCA'G
Cuts at: 765 1546 2231 765
Size: 781 685 3033
Fragments arranged by size:
3033 781 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4499
PvuII CAG'CTG
Cuts at: 754 868 1924 2284 754
Size: 114 1056 360 2969
Fragments arranged by size:
2969 1056 360 114
RsrII CG'GwC_CG
Cuts at: 2694 2694
Size: 4499
SacI G_AGCT'C
Cuts at: 1513 1513
Size: 4499
SalI G'TCGA_C
Cuts at: 1536 1536
Size: 4499
SapI GCTCTTCn'nnn_
Cuts at: 2522 2732 2522
Size: 210 4289
SbfI CC_TGCA'GG
Cuts at: 1546 1546
Size: 4499
ScaI AGT'ACT
Cuts at: 4016 4016
Size: 4499
SfcI C'TryA_G
Cuts at: 33 267 761 1035 1542 2227 3220 3411 33
Size: 234 494 274 507 685 993 191 1121
Fragments arranged by size:
1121 993 685 507 494 274 234 191
SfoI GGC'GCC
Cuts at: 1368 1931 2179 1368
Size: 563 248 3688
Fragments arranged by size:
3688 563 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4499
SmaI CCC'GGG
Cuts at: 1521 1521
Size: 4499
SmlI C'TyrA_G
Cuts at: 1654 2569 3061 3323 1654
Size: 915 492 262 2830
Fragments arranged by size:
2830 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4499
SphI G_CATG'C
Cuts at: 316 1552 2052 316
Size: 1236 500 2763
Fragments arranged by size:
2763 1236 500
SspI AAT'ATT
Cuts at: 246 246
Size: 4499
StuI AGG'CCT
Cuts at: 561 1053 561
Size: 492 4007
StyI C'CwwG_G
Cuts at: 182 260 1633 1987 182
Size: 78 1373 354 2694
Fragments arranged by size:
2694 1373 354 78
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 516 704 1438 2082 2215 2674 2883 516
Size: 188 734 644 133 459 209 2132
Fragments arranged by size:
2132 734 644 459 209 188 133
TatI w'GTAC_w
Cuts at: 4014 4014
Size: 4499
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1092 1124 4016 4048 1092
Size: 32 2892 32 1543
Fragments arranged by size:
2892 1543 32 32
Tth111I GACn'n_nGTC
Cuts at: 2296 2296
Size: 4499
XbaI T'CTAG_A
Cuts at: 1530 1530
Size: 4499
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1994 1994
Size: 4499
XmaI C'CCGG_G
Cuts at: 1519 1519
Size: 4499
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BcgI BciVI BclI BfrBI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII
HindIII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NsiI NspI PciI PmeI
PmlI PpiI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI ApaI
AscI AvrII BaeI BbvCI BglI BmgBI BmtI BsaXI
BsgI BsiWI BsmBI BsrGI BssHII BstEII BstXI ClaI
CspCI DraIII EcoNI FseI FspAI HpaI MfeI NdeI
NheI NotI NruI PacI PasI PflMI PfoI PpuMI
PshAI PsiI PspOMI PspXI PsrI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI SwaI XhoI XmnI ZraI