Restriction map for
pF1KB5863
(Circular) (Six-base) MAPSORT of: pF1KB5863.seq Check: 8870 from: 1 to: 4496
pF1KB5863 4496 bp
With 182 enzymes: SgfI *
October 7, 2009 14:18 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 774 774
Size: 4496
AccI GT'mk_AC
Cuts at: 368 1534 368
Size: 1166 3330
Acc65I G'GTAC_C
Cuts at: 1512 1512
Size: 4496
AclI AA'CG_TT
Cuts at: 4336 4336
Size: 4496
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 444 2321 2753 3500 3768 444
Size: 1877 432 747 268 1172
Fragments arranged by size:
1877 1172 747 432 268
AflIII A'CryG_T
Cuts at: 1848 2952 3929 1848
Size: 1104 977 2415
Fragments arranged by size:
2415 1104 977
AgeI A'CCGG_T
Cuts at: 2884 2884
Size: 4496
AlwNI CAG_nnn'CTG
Cuts at: 216 482 779 1801 3368 216
Size: 266 297 1022 1567 1344
Fragments arranged by size:
1567 1344 1022 297 266
ApaI G_GGCC'C
Cuts at: 1322 1322
Size: 4496
ApaLI G'TGCA_C
Cuts at: 3266 3266
Size: 4496
ApoI r'AATT_y
Cuts at: 152 320 1500 3849 4105 152
Size: 168 1180 2349 256 543
Fragments arranged by size:
2349 1180 543 256 168
AseI AT'TA_AT
Cuts at: 20 20
Size: 4496
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4496
AvaI C'yCGr_G
Cuts at: 1279 1516 1279
Size: 237 4259
BamHI G'GATC_C
Cuts at: 221 1439 1521 221
Size: 1218 82 3196
Fragments arranged by size:
3196 1218 82
BanI G'GyrC_C
Cuts at: 1342 1485 1512 1926 2174 2209 1342
Size: 143 27 414 248 35 3629
Fragments arranged by size:
3629 414 248 143 35 27
BanII G_rGCy'C
Cuts at: 257 1097 1322 1391 1510 2540 257
Size: 840 225 69 119 1030 2213
Fragments arranged by size:
2213 1030 840 225 119 69
BbeI G_GCGC'C
Cuts at: 1930 2178 1930
Size: 248 4248
BbsI GAAGACnn'nnnn_
Cuts at: 719 728 954 719
Size: 9 226 4261
Fragments arranged by size:
4261 226 9
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 423 457 423
Size: 34 4462
BciVI GTATCCnnnnn_n'
Cuts at: 2394 3155 4496 2394
Size: 761 1341 2394
Fragments arranged by size:
2394 1341 761
BclI T'GATC_A
Cuts at: 2016 2016
Size: 4496
BglI GCCn_nnn'nGGC
Cuts at: 479 823 479
Size: 344 4152
BglII A'GATC_T
Cuts at: 293 2011 4048 293
Size: 1718 2037 741
Fragments arranged by size:
2037 1718 741
BlpI GC'TnA_GC
Cuts at: 1608 1608
Size: 4496
Bme1580I G_kGCm'C
Cuts at: 207 431 1058 1322 1451 1488 2121 2214 3270
Size: 224 627 264 129 37 633 93 1056
Cuts at: 3270 207
Size: 1433
Fragments arranged by size:
1433 1056 633 627 264 224 129 93
37
BmgBI CAC'GTC
Cuts at: 366 366
Size: 4496
BmrI ACTGGGnnnn_n'
Cuts at: 1708 1789 2124 4268 1708
Size: 81 335 2144 1936
Fragments arranged by size:
2144 1936 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2863 2863
Size: 4496
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 174 268 292 174
Size: 94 24 4378
Fragments arranged by size:
4378 94 24
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1672 2551 3043 3341 1672
Size: 879 492 298 2827
Fragments arranged by size:
2827 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1108 4200 1108
Size: 3092 1404
BsaAI yAC'GTr
Cuts at: 437 1849 437
Size: 1412 3084
BsaBI GATnn'nnATC
Cuts at: 6 2010 4047 6
Size: 2004 2037 455
Fragments arranged by size:
2037 2004 455
BsaHI Gr'CG_yC
Cuts at: 1927 2175 2877 4373 1927
Size: 248 702 1496 2050
Fragments arranged by size:
2050 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1685 2206 2884 3158 3305 3716 1685
Size: 521 678 274 147 411 2465
Fragments arranged by size:
2465 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 153 183 265 295 391 421 153
Size: 30 82 30 96 30 4228
Fragments arranged by size:
4228 96 82 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 161 265 304 322 580 161
Size: 104 39 18 258 4077
Fragments arranged by size:
4077 258 104 39 18
BseYI C'CCAG_C
Cuts at: 482 609 923 1025 1130 1370 1481 1921 2679
Size: 127 314 102 105 240 111 440 758
Cuts at: 2679 3256 3752 482
Size: 577 496 1226
Fragments arranged by size:
1226 758 577 496 440 314 240 127
111 105 102
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 531 1000 1245 531
Size: 469 245 3782
Fragments arranged by size:
3782 469 245
BsiEI CG_ry'CG
Cuts at: 104 2038 2084 3292 104
Size: 1934 46 1208 1308
Fragments arranged by size:
1934 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 257 404 833 991 1097 1510 2288 2478 3270
Size: 147 429 158 106 413 778 190 792
Cuts at: 3270 257
Size: 1483
Fragments arranged by size:
1483 792 778 429 413 190 158 147
106
Bsp1286I G_dGCh'C
Cuts at: 207 257 404 431 833 991 1058 1097 1322
Size: 50 147 27 402 158 67 39 225
Cuts at: 1322 1391 1451 1488 1510 2121 2214 2288 2478
Size: 69 60 37 22 611 93 74 190
Cuts at: 2478 2540 3270 207
Size: 62 730 1433
Fragments arranged by size:
1433 730 611 402 225 190 158 147
93 74 69 67 62 60 50 39
37 27 22
BspEI T'CCGG_A
Cuts at: 3716 3716
Size: 4496
BspHI T'CATG_A
Cuts at: 1235 3672 1235
Size: 2437 2059
BspMI ACCTGCnnnn'nnnn_
Cuts at: 774 923 1546 1760 2062 2443 774
Size: 149 623 214 302 381 2827
Fragments arranged by size:
2827 623 381 302 214 149
BsrBI CCG'CTC
Cuts at: 50 276 2788 2842 4326 4491 50
Size: 226 2512 54 1484 165 55
Fragments arranged by size:
2512 1484 226 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2408 2408
Size: 4496
BsrFI r'CCGG_y
Cuts at: 1105 1286 2494 2675 2884 1105
Size: 181 1208 181 209 2717
Fragments arranged by size:
2717 1208 209 181 181
BssSI C'ACGA_G
Cuts at: 1120 2767 3125 1120
Size: 1647 358 2491
Fragments arranged by size:
2491 1647 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1873 1873
Size: 4496
BstBI TT'CG_AA
Cuts at: 2857 2857
Size: 4496
BstEII G'GTnAC_C
Cuts at: 373 373
Size: 4496
BstYI r'GATC_y
Cuts at: 1 221 293 732 1439 1521 1988 2011 2346
Size: 220 72 439 707 82 467 23 335
Cuts at: 2346 2592 3604 3690 3702 4048 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1012 707 467 449 439 346 335 246
220 86 82 72 23 12
BtgI C'CryG_G
Cuts at: 736 1205 1322 1338 1443 736
Size: 469 117 16 105 3789
Fragments arranged by size:
3789 469 117 105 16
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 990 2446 2627 2790 990
Size: 1456 181 163 2696
Fragments arranged by size:
2696 1456 181 163
BtsI GCAGTG_nn'
Cuts at: 634 647 833 864 1009 1739 634
Size: 13 186 31 145 730 3391
Fragments arranged by size:
3391 730 186 145 31 13
DraI TTT'AAA
Cuts at: 1495 1495
Size: 4496
DraIII CAC_nnn'GTG
Cuts at: 213 213
Size: 4496
DrdI GACnn_nn'nnGTC
Cuts at: 2202 3060 2202
Size: 858 3638
EaeI y'GGCC_r
Cuts at: 140 1107 2081 2255 2646 2673 4018 140
Size: 967 974 174 391 27 1345 618
Fragments arranged by size:
1345 974 967 618 391 174 27
EagI C'GGCC_G
Cuts at: 2081 2081
Size: 4496
EarI CTCTTCn'nnn_
Cuts at: 1471 2519 2729 1471
Size: 1048 210 3238
Fragments arranged by size:
3238 1048 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2704 3014 3160 4306 2704
Size: 310 146 1146 2894
Fragments arranged by size:
2894 1146 310 146
EcoICRI GAG'CTC
Cuts at: 255 1095 1508 255
Size: 840 413 3243
Fragments arranged by size:
3243 840 413
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 174 268 292 444 2321 2753 3500 3768 174
Size: 94 24 152 1877 432 747 268 902
Fragments arranged by size:
1877 902 747 432 268 152 94 24
EcoNI CCTnn'n_nnAGG
Cuts at: 565 565
Size: 4496
EcoO109I rG'GnC_Cy
Cuts at: 662 1318 1635 662
Size: 656 317 3523
Fragments arranged by size:
3523 656 317
EcoRI G'AATT_C
Cuts at: 1500 1500
Size: 4496
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 978 2515 978
Size: 1537 2959
FseI GG_CCGG'CC
Cuts at: 1109 1109
Size: 4496
FspI TGC'GCA
Cuts at: 2277 2277
Size: 4496
HaeII r_GCGC'y
Cuts at: 585 924 1930 2178 3200 4179 585
Size: 339 1006 248 1022 979 902
Fragments arranged by size:
1022 1006 979 902 339 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2338 2368 2370 2400 2338
Size: 30 2 30 4434
Fragments arranged by size:
4434 30 30 2
HincII GTy'rAC
Cuts at: 369 1535 369
Size: 1166 3330
KasI G'GCGC_C
Cuts at: 1926 2174 1926
Size: 248 4248
KpnI G_GTAC'C
Cuts at: 1516 1516
Size: 4496
MfeI C'AATT_G
Cuts at: 522 522
Size: 4496
MluI A'CGCG_T
Cuts at: 3929 3929
Size: 4496
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3167 3351 3167
Size: 184 4312
MscI TGG'CCA
Cuts at: 142 2257 142
Size: 2115 2381
MslI CAynn'nnrTG
Cuts at: 2485 2612 2485
Size: 127 4369
MspA1I CmG'CkG
Cuts at: 464 479 738 859 1076 1607 1921 2281 3294
Size: 15 259 121 217 531 314 360 1013
Cuts at: 3294 3539 464
Size: 245 1421
Fragments arranged by size:
1421 1013 531 360 314 259 245 217
121 15
NaeI GCC'GGC
Cuts at: 1107 2677 1107
Size: 1570 2926
NarI GG'CG_CC
Cuts at: 1927 2175 1927
Size: 248 4248
NcoI C'CATG_G
Cuts at: 1322 1443 1322
Size: 121 4375
NgoMIV G'CCGG_C
Cuts at: 1105 2675 1105
Size: 1570 2926
NspI r_CATG'y
Cuts at: 1263 1549 2049 2956 1263
Size: 286 500 907 2803
Fragments arranged by size:
2803 907 500 286
PciI A'CATG_T
Cuts at: 2952 2952
Size: 4496
PflMI CCAn_nnn'nTGG
Cuts at: 333 384 870 333
Size: 51 486 3959
Fragments arranged by size:
3959 486 51
PfoI T'CCnGG_A
Cuts at: 245 1067 245
Size: 822 3674
PmeI GTTT'AAAC
Cuts at: 1495 1495
Size: 4496
PmlI CAC'GTG
Cuts at: 437 437
Size: 4496
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3635 3667 3635
Size: 32 4464
PspOMI G'GGCC_C
Cuts at: 1318 1318
Size: 4496
PstI C_TGCA'G
Cuts at: 629 1543 2228 629
Size: 914 685 2897
Fragments arranged by size:
2897 914 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4496
PvuII CAG'CTG
Cuts at: 464 1921 2281 464
Size: 1457 360 2679
Fragments arranged by size:
2679 1457 360
RsrII CG'GwC_CG
Cuts at: 2691 2691
Size: 4496
SacI G_AGCT'C
Cuts at: 257 1097 1510 257
Size: 840 413 3243
Fragments arranged by size:
3243 840 413
SacII CC_GC'GG
Cuts at: 739 739
Size: 4496
SalI G'TCGA_C
Cuts at: 367 1533 367
Size: 1166 3330
SapI GCTCTTCn'nnn_
Cuts at: 1471 2519 2729 1471
Size: 1048 210 3238
Fragments arranged by size:
3238 1048 210
SbfI CC_TGCA'GG
Cuts at: 1543 1543
Size: 4496
ScaI AGT'ACT
Cuts at: 4013 4013
Size: 4496
SexAI A'CCwGG_T
Cuts at: 209 1345 209
Size: 1136 3360
SfcI C'TryA_G
Cuts at: 33 585 625 978 1539 2224 3217 3408 33
Size: 552 40 353 561 685 993 191 1121
Fragments arranged by size:
1121 993 685 561 552 353 191 40
SfoI GGC'GCC
Cuts at: 1928 2176 1928
Size: 248 4248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4496
SmaI CCC'GGG
Cuts at: 1518 1518
Size: 4496
SmlI C'TyrA_G
Cuts at: 1651 2566 3058 3320 1651
Size: 915 492 262 2827
Fragments arranged by size:
2827 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4496
SphI G_CATG'C
Cuts at: 1549 2049 1549
Size: 500 3996
StyI C'CwwG_G
Cuts at: 314 395 1322 1443 1630 1984 314
Size: 81 927 121 187 354 2826
Fragments arranged by size:
2826 927 354 187 121 81
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 837 2079 2212 2671 2880 837
Size: 1242 133 459 209 2453
Fragments arranged by size:
2453 1242 459 209 133
TatI w'GTAC_w
Cuts at: 4011 4011
Size: 4496
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 153 185 265 297 391 423 4013 4045 153
Size: 32 80 32 94 32 3590 32 604
Fragments arranged by size:
3590 604 94 80 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2293 2293
Size: 4496
XbaI T'CTAG_A
Cuts at: 1527 1527
Size: 4496
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 243 1991 243
Size: 1748 2748
XmaI C'CCGG_G
Cuts at: 1516 1516
Size: 4496
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FseI FspI
HaeII Hin4I HincII KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PciI PflMI PfoI PmeI PmlI PpiI PspOMI PstI
PvuI PvuII RsrII SacI SacII SalI SapI SbfI
ScaI SexAI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AloI AscI
AvrII BaeI BbvCI BfrBI BmtI Bpu10I BsiWI BsmI
BsmBI BsrGI BssHII BstXI BstZ17I Bsu36I ClaI CspCI
EcoRV FspAI HindIII HpaI NdeI NheI NotI NruI
NsiI PacI PasI PpuMI PshAI PsiI PspXI PsrI
SanDI SfiI SgrAI SnaBI SrfI SspI StuI SwaI
XhoI XmnI ZraI