Restriction map for
pF1KB5854
(Circular) (Six-base) MAPSORT of: pF1KB5854.seq Check: 6436 from: 1 to: 4577
pF1KB5854 4577 bp
With 182 enzymes: SgfI *
September 25, 2012 15:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 442 442
Size: 4577
AccI GT'mk_AC
Cuts at: 1615 1615
Size: 4577
Acc65I G'GTAC_C
Cuts at: 1593 1593
Size: 4577
AclI AA'CG_TT
Cuts at: 4417 4417
Size: 4577
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2402 2834 3581 3849 2402
Size: 432 747 268 3130
Fragments arranged by size:
3130 747 432 268
AflIII A'CryG_T
Cuts at: 1310 1929 3033 4010 1310
Size: 619 1104 977 1877
Fragments arranged by size:
1877 1104 977 619
AgeI A'CCGG_T
Cuts at: 1043 2965 1043
Size: 1922 2655
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1361 1361
Size: 4577
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 163 195 163
Size: 32 4545
AlwNI CAG_nnn'CTG
Cuts at: 636 858 1882 3449 636
Size: 222 1024 1567 1764
Fragments arranged by size:
1764 1567 1024 222
ApaI G_GGCC'C
Cuts at: 614 1372 614
Size: 758 3819
ApaLI G'TGCA_C
Cuts at: 1012 3347 1012
Size: 2335 2242
ApoI r'AATT_y
Cuts at: 343 1581 3930 4186 343
Size: 1238 2349 256 734
Fragments arranged by size:
2349 1238 734 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4577
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4577
AvaI C'yCGr_G
Cuts at: 166 421 1138 1597 166
Size: 255 717 459 3146
Fragments arranged by size:
3146 717 459 255
BamHI G'GATC_C
Cuts at: 1602 1602
Size: 4577
BanI G'GyrC_C
Cuts at: 113 1122 1251 1593 2007 2255 2290 113
Size: 1009 129 342 414 248 35 2400
Fragments arranged by size:
2400 1009 414 342 248 129 35
BanII G_rGCy'C
Cuts at: 614 1372 1591 2621 614
Size: 758 219 1030 2570
Fragments arranged by size:
2570 1030 758 219
BbeI G_GCGC'C
Cuts at: 117 1126 2011 2259 117
Size: 1009 885 248 2435
Fragments arranged by size:
2435 1009 885 248
BbsI GAAGACnn'nnnn_
Cuts at: 1183 1183
Size: 4577
BciVI GTATCCnnnnn_n'
Cuts at: 2475 3236 4577 2475
Size: 761 1341 2475
Fragments arranged by size:
2475 1341 761
BclI T'GATC_A
Cuts at: 2097 2097
Size: 4577
BglI GCCn_nnn'nGGC
Cuts at: 148 148
Size: 4577
BglII A'GATC_T
Cuts at: 2092 4129 2092
Size: 2037 2540
BlpI GC'TnA_GC
Cuts at: 1689 1689
Size: 4577
Bme1580I G_kGCm'C
Cuts at: 614 1016 1256 1372 1440 2202 2295 3351 614
Size: 402 240 116 68 762 93 1056 1840
Fragments arranged by size:
1840 1056 762 402 240 116 93 68
BmgBI CAC'GTC
Cuts at: 1377 1377
Size: 4577
BmrI ACTGGGnnnn_n'
Cuts at: 1158 1789 1870 2205 4349 1158
Size: 631 81 335 2144 1386
Fragments arranged by size:
2144 1386 631 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 309 2944 309
Size: 2635 1942
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1267 1267
Size: 4577
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1075 1222 1468 1753 2632 3124 3422 1075
Size: 147 246 285 879 492 298 2230
Fragments arranged by size:
2230 879 492 298 285 246 147
BsaI GGTCTCn'nnnn_
Cuts at: 280 373 1051 4281 280
Size: 93 678 3230 576
Fragments arranged by size:
3230 678 576 93
BsaAI yAC'GTr
Cuts at: 1260 1930 1260
Size: 670 3907
BsaBI GATnn'nnATC
Cuts at: 6 2091 4128 6
Size: 2085 2037 455
Fragments arranged by size:
2085 2037 455
BsaHI Gr'CG_yC
Cuts at: 114 1123 2008 2256 2958 4454 114
Size: 1009 885 248 702 1496 237
Fragments arranged by size:
1496 1009 885 702 248 237
BsaWI w'CCGG_w
Cuts at: 977 1043 1766 2287 2965 3239 3386 3797 977
Size: 66 723 521 678 274 147 411 1757
Fragments arranged by size:
1757 723 678 521 411 274 147 66
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1545 1575 1545
Size: 30 4547
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1009 1009
Size: 4577
BseYI C'CCAG_C
Cuts at: 1421 2002 2760 3337 3833 1421
Size: 581 758 577 496 2165
Fragments arranged by size:
2165 758 581 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 106 420 106
Size: 314 4263
BsiEI CG_ry'CG
Cuts at: 104 2119 2165 3373 104
Size: 2015 46 1208 1308
Fragments arranged by size:
2015 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 556 1016 1085 1591 2369 2559 3351 556
Size: 460 69 506 778 190 792 1782
Fragments arranged by size:
1782 792 778 506 460 190 69
BsmI GAATG_Cn'
Cuts at: 599 832 599
Size: 233 4344
Bsp1286I G_dGCh'C
Cuts at: 556 614 1016 1085 1256 1372 1440 1591 2202
Size: 58 402 69 171 116 68 151 611
Cuts at: 2202 2295 2369 2559 2621 3351 556
Size: 93 74 190 62 730 1782
Fragments arranged by size:
1782 730 611 402 190 171 151 116
93 74 69 68 62 58
BspEI T'CCGG_A
Cuts at: 3797 3797
Size: 4577
BspHI T'CATG_A
Cuts at: 3753 3753
Size: 4577
BspMI ACCTGCnnnn'nnnn_
Cuts at: 298 442 764 1627 1841 2143 2524 298
Size: 144 322 863 214 302 381 2351
Fragments arranged by size:
2351 863 381 322 302 214 144
BsrBI CCG'CTC
Cuts at: 50 1193 2869 2923 4407 4572 50
Size: 1143 1676 54 1484 165 55
Fragments arranged by size:
1676 1484 1143 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2489 2489
Size: 4577
BsrFI r'CCGG_y
Cuts at: 1043 1220 1321 2575 2756 2965 1043
Size: 177 101 1254 181 209 2655
Fragments arranged by size:
2655 1254 209 181 177 101
BssSI C'ACGA_G
Cuts at: 222 529 1015 1210 2848 3206 222
Size: 307 486 195 1638 358 1593
Fragments arranged by size:
1638 1593 486 358 307 195
BstAPI GCAn_nnn'nTGC
Cuts at: 596 858 1274 1954 596
Size: 262 416 680 3219
Fragments arranged by size:
3219 680 416 262
BstBI TT'CG_AA
Cuts at: 455 2938 455
Size: 2483 2094
BstEII G'GTnAC_C
Cuts at: 1470 1470
Size: 4577
BstXI CCAn_nnnn'nTGG
Cuts at: 813 813
Size: 4577
BstYI r'GATC_y
Cuts at: 1 1457 1602 2069 2092 2427 2673 3685 3771
Size: 1456 145 467 23 335 246 1012 86
Cuts at: 3771 3783 4129 1
Size: 12 346 449
Fragments arranged by size:
1456 1012 467 449 346 335 246 145
86 23 12
BtgI C'CryG_G
Cuts at: 107 564 107
Size: 457 4120
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1059 2527 2708 2871 1059
Size: 1468 181 163 2765
Fragments arranged by size:
2765 1468 181 163
BtsI GCAGTG_nn'
Cuts at: 118 1820 118
Size: 1702 2875
DraI TTT'AAA
Cuts at: 1576 1576
Size: 4577
DraIII CAC_nnn'GTG
Cuts at: 1020 1020
Size: 4577
DrdI GACnn_nn'nnGTC
Cuts at: 2283 3141 2283
Size: 858 3719
EaeI y'GGCC_r
Cuts at: 2162 2336 2727 2754 4099 2162
Size: 174 391 27 1345 2640
Fragments arranged by size:
2640 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2162 2162
Size: 4577
EarI CTCTTCn'nnn_
Cuts at: 239 278 296 643 2600 2810 239
Size: 39 18 347 1957 210 2006
Fragments arranged by size:
2006 1957 347 210 39 18
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2785 3095 3241 4387 2785
Size: 310 146 1146 2975
Fragments arranged by size:
2975 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1589 1589
Size: 4577
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1267 2402 2834 3581 3849 1267
Size: 1135 432 747 268 1995
Fragments arranged by size:
1995 1135 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 610 611 687 855 1368 1716 610
Size: 1 76 168 513 348 3471
Fragments arranged by size:
3471 513 348 168 76 1
EcoRI G'AATT_C
Cuts at: 343 1581 343
Size: 1238 3339
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2596 2596
Size: 4577
FspI TGC'GCA
Cuts at: 2358 2358
Size: 4577
HaeII r_GCGC'y
Cuts at: 117 1126 2011 2259 3281 4260 117
Size: 1009 885 248 1022 979 434
Fragments arranged by size:
1022 1009 979 885 434 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2419 2449 2451 2481 2419
Size: 30 2 30 4515
Fragments arranged by size:
4515 30 30 2
HincII GTy'rAC
Cuts at: 1616 1616
Size: 4577
KasI G'GCGC_C
Cuts at: 113 1122 2007 2255 113
Size: 1009 885 248 2435
Fragments arranged by size:
2435 1009 885 248
KpnI G_GTAC'C
Cuts at: 1597 1597
Size: 4577
MluI A'CGCG_T
Cuts at: 4010 4010
Size: 4577
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3248 3432 3248
Size: 184 4393
MscI TGG'CCA
Cuts at: 2338 2338
Size: 4577
MslI CAynn'nnrTG
Cuts at: 1078 1117 1165 2566 2693 1078
Size: 39 48 1401 127 2962
Fragments arranged by size:
2962 1401 127 48 39
MspA1I CmG'CkG
Cuts at: 566 680 1274 1364 1421 1688 2002 2362 3375
Size: 114 594 90 57 267 314 360 1013
Cuts at: 3375 3620 566
Size: 245 1523
Fragments arranged by size:
1523 1013 594 360 314 267 245 114
90 57
NaeI GCC'GGC
Cuts at: 1222 1323 2758 1222
Size: 101 1435 3041
Fragments arranged by size:
3041 1435 101
NarI GG'CG_CC
Cuts at: 114 1123 2008 2256 114
Size: 1009 885 248 2435
Fragments arranged by size:
2435 1009 885 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 4577
NdeI CA'TA_TG
Cuts at: 714 714
Size: 4577
NgoMIV G'CCGG_C
Cuts at: 1220 1321 2756 1220
Size: 101 1435 3041
Fragments arranged by size:
3041 1435 101
NspI r_CATG'y
Cuts at: 1314 1630 2130 3037 1314
Size: 316 500 907 2854
Fragments arranged by size:
2854 907 500 316
PciI A'CATG_T
Cuts at: 1310 3033 1310
Size: 1723 2854
PmeI GTTT'AAAC
Cuts at: 1576 1576
Size: 4577
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3716 3748 3716
Size: 32 4545
PspOMI G'GGCC_C
Cuts at: 610 1368 610
Size: 758 3819
PstI C_TGCA'G
Cuts at: 496 1624 2309 496
Size: 1128 685 2764
Fragments arranged by size:
2764 1128 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4577
PvuII CAG'CTG
Cuts at: 1274 1421 2002 2362 1274
Size: 147 581 360 3489
Fragments arranged by size:
3489 581 360 147
RsrII CG'GwC_CG
Cuts at: 2772 2772
Size: 4577
SacI G_AGCT'C
Cuts at: 1591 1591
Size: 4577
SacII CC_GC'GG
Cuts at: 567 567
Size: 4577
SalI G'TCGA_C
Cuts at: 1614 1614
Size: 4577
SapI GCTCTTCn'nnn_
Cuts at: 2600 2810 2600
Size: 210 4367
SbfI CC_TGCA'GG
Cuts at: 1624 1624
Size: 4577
ScaI AGT'ACT
Cuts at: 4094 4094
Size: 4577
SfcI C'TryA_G
Cuts at: 33 492 1620 2305 3298 3489 33
Size: 459 1128 685 993 191 1121
Fragments arranged by size:
1128 1121 993 685 459 191
SfoI GGC'GCC
Cuts at: 115 1124 2009 2257 115
Size: 1009 885 248 2435
Fragments arranged by size:
2435 1009 885 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4577
SmaI CCC'GGG
Cuts at: 1599 1599
Size: 4577
SmlI C'TyrA_G
Cuts at: 1090 1237 1483 1732 2647 3139 3401 1090
Size: 147 246 249 915 492 262 2266
Fragments arranged by size:
2266 915 492 262 249 246 147
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4577
SphI G_CATG'C
Cuts at: 1630 2130 1630
Size: 500 4077
StuI AGG'CCT
Cuts at: 595 595
Size: 4577
StyI C'CwwG_G
Cuts at: 107 1466 1523 1711 2065 107
Size: 1359 57 188 354 2619
Fragments arranged by size:
2619 1359 354 188 57
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1222 1318 2160 2293 2752 2961 1222
Size: 96 842 133 459 209 2838
Fragments arranged by size:
2838 842 459 209 133 96
TatI w'GTAC_w
Cuts at: 4092 4092
Size: 4577
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 826 858 4094 4126 826
Size: 32 3236 32 1277
Fragments arranged by size:
3236 1277 32 32
Tth111I GACn'n_nGTC
Cuts at: 2374 2374
Size: 4577
XbaI T'CTAG_A
Cuts at: 1608 1608
Size: 4577
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 207 2072 207
Size: 1865 2712
XmaI C'CCGG_G
Cuts at: 1597 1597
Size: 4577
XmnI GAAnn'nnTTC
Cuts at: 343 748 1312 343
Size: 405 564 3608
Fragments arranged by size:
3608 564 405
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlfI
AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NspI PciI PmeI PpiI PspOMI PstI PvuI PvuII
RsrII SacI SacII SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AscI AvrII BaeI
BbvCI BcgI BfrBI BmtI Bpu10I BsiWI BsmBI BsrGI
BssHII BstZ17I Bsu36I ClaI CspCI EcoNI EcoRV FseI
FspAI HindIII HpaI MfeI NheI NotI NruI NsiI
PacI PasI PflMI PfoI PmlI PpuMI PshAI PsiI
PspXI PsrI SanDI SexAI SfiI SgrAI SnaBI SrfI
SspI SwaI XhoI ZraI