Restriction map for
pF1KB5852
(Circular) (Six-base) MAPSORT of: pF1KB5852.seq Check: 683 from: 1 to: 4520
pF1KB5852 4520 bp
With 182 enzymes: SgfI *
October 7, 2009 14:17 ..
AatII G_ACGT'C
Cuts at: 197 197
Size: 4520
AccI GT'mk_AC
Cuts at: 954 1558 954
Size: 604 3916
Acc65I G'GTAC_C
Cuts at: 1536 1536
Size: 4520
AclI AA'CG_TT
Cuts at: 4360 4360
Size: 4520
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 758 2345 2777 3524 3792 758
Size: 1587 432 747 268 1486
Fragments arranged by size:
1587 1486 747 432 268
AflIII A'CryG_T
Cuts at: 1872 2976 3953 1872
Size: 1104 977 2439
Fragments arranged by size:
2439 1104 977
AgeI A'CCGG_T
Cuts at: 431 2908 431
Size: 2477 2043
AlwNI CAG_nnn'CTG
Cuts at: 1825 3392 1825
Size: 1567 2953
ApaLI G'TGCA_C
Cuts at: 703 3290 703
Size: 2587 1933
ApoI r'AATT_y
Cuts at: 227 1153 1168 1182 1524 3873 4129 227
Size: 926 15 14 342 2349 256 618
Fragments arranged by size:
2349 926 618 342 256 15 14
AseI AT'TA_AT
Cuts at: 20 358 20
Size: 338 4182
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4520
AvaI C'yCGr_G
Cuts at: 1540 1540
Size: 4520
BamHI G'GATC_C
Cuts at: 1545 1545
Size: 4520
BanI G'GyrC_C
Cuts at: 421 1536 1950 2198 2233 421
Size: 1115 414 248 35 2708
Fragments arranged by size:
2708 1115 414 248 35
BanII G_rGCy'C
Cuts at: 542 1287 1534 2564 542
Size: 745 247 1030 2498
Fragments arranged by size:
2498 1030 745 247
BbeI G_GCGC'C
Cuts at: 1954 2202 1954
Size: 248 4272
BbsI GAAGACnn'nnnn_
Cuts at: 197 267 197
Size: 70 4450
BciVI GTATCCnnnnn_n'
Cuts at: 2418 3179 4520 2418
Size: 761 1341 2418
Fragments arranged by size:
2418 1341 761
BclI T'GATC_A
Cuts at: 2040 2040
Size: 4520
BglII A'GATC_T
Cuts at: 2035 4072 2035
Size: 2037 2483
BlpI GC'TnA_GC
Cuts at: 1632 1632
Size: 4520
Bme1580I G_kGCm'C
Cuts at: 707 2145 2238 3294 707
Size: 1438 93 1056 1933
Fragments arranged by size:
1933 1438 1056 93
BmgBI CAC'GTC
Cuts at: 702 1481 702
Size: 779 3741
BmrI ACTGGGnnnn_n'
Cuts at: 1123 1283 1732 1813 2148 4292 1123
Size: 160 449 81 335 2144 1351
Fragments arranged by size:
2144 1351 449 335 160 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2887 2887
Size: 4520
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 556 556
Size: 4520
Bpu10I CC'TnA_GC
Cuts at: 1373 1373
Size: 4520
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 394 1075 1227 1696 2575 3067 3365 394
Size: 681 152 469 879 492 298 1549
Fragments arranged by size:
1549 879 681 492 469 298 152
BsaI GGTCTCn'nnnn_
Cuts at: 4224 4224
Size: 4520
BsaAI yAC'GTr
Cuts at: 1873 1873
Size: 4520
BsaBI GATnn'nnATC
Cuts at: 6 920 2034 4071 6
Size: 914 1114 2037 455
Fragments arranged by size:
2037 1114 914 455
BsaHI Gr'CG_yC
Cuts at: 194 1951 2199 2901 4397 194
Size: 1757 248 702 1496 317
Fragments arranged by size:
1757 1496 702 317 248
BsaWI w'CCGG_w
Cuts at: 431 1709 2230 2908 3182 3329 3740 431
Size: 1278 521 678 274 147 411 1211
Fragments arranged by size:
1278 1211 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 846 876 846
Size: 30 4490
BseYI C'CCAG_C
Cuts at: 1945 2703 3280 3776 1945
Size: 758 577 496 2689
Fragments arranged by size:
2689 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 2062 2108 3316 104
Size: 1958 46 1208 1308
Fragments arranged by size:
1958 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 542 707 1382 1534 2312 2502 3294 542
Size: 165 675 152 778 190 792 1768
Fragments arranged by size:
1768 792 778 675 190 165 152
BsiWI C'GTAC_G
Cuts at: 238 238
Size: 4520
Bsp1286I G_dGCh'C
Cuts at: 542 707 1287 1382 1534 2145 2238 2312 2502
Size: 165 580 95 152 611 93 74 190
Cuts at: 2502 2564 3294 542
Size: 62 730 1768
Fragments arranged by size:
1768 730 611 580 190 165 152 95
93 74 62
BspEI T'CCGG_A
Cuts at: 3740 3740
Size: 4520
BspHI T'CATG_A
Cuts at: 3696 3696
Size: 4520
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1570 1784 2086 2467 1570
Size: 214 302 381 3623
Fragments arranged by size:
3623 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2812 2866 4350 4515 50
Size: 2762 54 1484 165 55
Fragments arranged by size:
2762 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2432 2432
Size: 4520
BsrFI r'CCGG_y
Cuts at: 431 2518 2699 2908 431
Size: 2087 181 209 2043
Fragments arranged by size:
2087 2043 209 181
BsrGI T'GTAC_A
Cuts at: 176 176
Size: 4520
BssSI C'ACGA_G
Cuts at: 2791 3149 2791
Size: 358 4162
BstAPI GCAn_nnn'nTGC
Cuts at: 1897 1897
Size: 4520
BstBI TT'CG_AA
Cuts at: 2881 2881
Size: 4520
BstXI CCAn_nnnn'nTGG
Cuts at: 171 171
Size: 4520
BstYI r'GATC_y
Cuts at: 1 327 668 1426 1545 2012 2035 2370 2616
Size: 326 341 758 119 467 23 335 246
Cuts at: 2616 3628 3714 3726 4072 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 758 467 449 346 341 335 326
246 119 86 23 12
BtgI C'CryG_G
Cuts at: 107 369 1021 107
Size: 262 652 3606
Fragments arranged by size:
3606 652 262
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2470 2651 2814 2470
Size: 181 163 4176
Fragments arranged by size:
4176 181 163
BtsI GCAGTG_nn'
Cuts at: 1208 1763 1208
Size: 555 3965
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 722 757 722
Size: 35 4485
DraI TTT'AAA
Cuts at: 1519 1519
Size: 4520
DrdI GACnn_nn'nnGTC
Cuts at: 2226 3084 2226
Size: 858 3662
EaeI y'GGCC_r
Cuts at: 149 454 566 2105 2279 2670 2697 4042 149
Size: 305 112 1539 174 391 27 1345 627
Fragments arranged by size:
1539 1345 627 391 305 174 112 27
EagI C'GGCC_G
Cuts at: 2105 2105
Size: 4520
EarI CTCTTCn'nnn_
Cuts at: 640 2543 2753 640
Size: 1903 210 2407
Fragments arranged by size:
2407 1903 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2728 3038 3184 4330 2728
Size: 310 146 1146 2918
Fragments arranged by size:
2918 1146 310 146
EcoICRI GAG'CTC
Cuts at: 540 1532 540
Size: 992 3528
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 556 758 2345 2777 3524 3792 556
Size: 202 1587 432 747 268 1284
Fragments arranged by size:
1587 1284 747 432 268 202
EcoO109I rG'GnC_Cy
Cuts at: 1209 1659 1209
Size: 450 4070
EcoRI G'AATT_C
Cuts at: 227 1168 1524 227
Size: 941 356 3223
Fragments arranged by size:
3223 941 356
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2539 2539
Size: 4520
FspI TGC'GCA
Cuts at: 2301 2301
Size: 4520
HaeII r_GCGC'y
Cuts at: 343 1954 2202 3224 4203 343
Size: 1611 248 1022 979 660
Fragments arranged by size:
1611 1022 979 660 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 524 556 2362 2392 2394 2424 524
Size: 32 1806 30 2 30 2620
Fragments arranged by size:
2620 1806 32 30 30 2
HincII GTy'rAC
Cuts at: 1493 1559 1493
Size: 66 4454
HindIII A'AGCT_T
Cuts at: 249 249
Size: 4520
HpaI GTT'AAC
Cuts at: 1493 1493
Size: 4520
KasI G'GCGC_C
Cuts at: 1950 2198 1950
Size: 248 4272
KpnI G_GTAC'C
Cuts at: 1540 1540
Size: 4520
MluI A'CGCG_T
Cuts at: 3953 3953
Size: 4520
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 889 3191 3375 889
Size: 2302 184 2034
Fragments arranged by size:
2302 2034 184
MscI TGG'CCA
Cuts at: 456 568 2281 456
Size: 112 1713 2695
Fragments arranged by size:
2695 1713 112
MslI CAynn'nnrTG
Cuts at: 172 610 2509 2636 172
Size: 438 1899 127 2056
Fragments arranged by size:
2056 1899 438 127
MspA1I CmG'CkG
Cuts at: 1631 1945 2305 3318 3563 1631
Size: 314 360 1013 245 2588
Fragments arranged by size:
2588 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2701 2701
Size: 4520
NarI GG'CG_CC
Cuts at: 1951 2199 1951
Size: 248 4272
NcoI C'CATG_G
Cuts at: 107 1021 107
Size: 914 3606
NgoMIV G'CCGG_C
Cuts at: 2699 2699
Size: 4520
NspI r_CATG'y
Cuts at: 171 1573 2073 2980 171
Size: 1402 500 907 1711
Fragments arranged by size:
1711 1402 907 500
PasI CC'CwG_GG
Cuts at: 114 114
Size: 4520
PciI A'CATG_T
Cuts at: 2976 2976
Size: 4520
PflMI CCAn_nnn'nTGG
Cuts at: 1046 1046
Size: 4520
PfoI T'CCnGG_A
Cuts at: 1278 1278
Size: 4520
PmeI GTTT'AAAC
Cuts at: 1519 1519
Size: 4520
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 676 708 3659 3691 676
Size: 32 2951 32 1505
Fragments arranged by size:
2951 1505 32 32
PpuMI rG'GwC_Cy
Cuts at: 1209 1209
Size: 4520
PsiI TTA'TAA
Cuts at: 1423 1423
Size: 4520
PstI C_TGCA'G
Cuts at: 578 1567 2252 578
Size: 989 685 2846
Fragments arranged by size:
2846 989 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4520
PvuII CAG'CTG
Cuts at: 1945 2305 1945
Size: 360 4160
RsrII CG'GwC_CG
Cuts at: 2715 2715
Size: 4520
SacI G_AGCT'C
Cuts at: 542 1534 542
Size: 992 3528
SalI G'TCGA_C
Cuts at: 1557 1557
Size: 4520
SapI GCTCTTCn'nnn_
Cuts at: 2543 2753 2543
Size: 210 4310
SbfI CC_TGCA'GG
Cuts at: 1567 1567
Size: 4520
ScaI AGT'ACT
Cuts at: 4037 4037
Size: 4520
SfcI C'TryA_G
Cuts at: 33 320 574 1450 1563 2248 3241 3432 33
Size: 287 254 876 113 685 993 191 1121
Fragments arranged by size:
1121 993 876 685 287 254 191 113
SfoI GGC'GCC
Cuts at: 1952 2200 1952
Size: 248 4272
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4520
SmaI CCC'GGG
Cuts at: 1542 1542
Size: 4520
SmlI C'TyrA_G
Cuts at: 409 1054 1242 1675 2590 3082 3344 409
Size: 645 188 433 915 492 262 1585
Fragments arranged by size:
1585 915 645 492 433 262 188
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4520
SphI G_CATG'C
Cuts at: 1573 2073 1573
Size: 500 4020
SspI AAT'ATT
Cuts at: 898 898
Size: 4520
StuI AGG'CCT
Cuts at: 1224 1224
Size: 4520
StyI C'CwwG_G
Cuts at: 107 1021 1654 2008 107
Size: 914 633 354 2619
Fragments arranged by size:
2619 914 633 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 177 858 2103 2236 2695 2904 177
Size: 681 1245 133 459 209 1793
Fragments arranged by size:
1793 1245 681 459 209 133
TatI w'GTAC_w
Cuts at: 176 4035 176
Size: 3859 661
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4037 4069 4037
Size: 32 4488
Tth111I GACn'n_nGTC
Cuts at: 2317 2317
Size: 4520
XbaI T'CTAG_A
Cuts at: 1158 1551 1158
Size: 393 4127
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 144 464 2015 144
Size: 320 1551 2649
Fragments arranged by size:
2649 1551 320
XmaI C'CCGG_G
Cuts at: 1540 1540
Size: 4520
ZraI GAC'GTC
Cuts at: 195 195
Size: 4520
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseYI BsiEI BsiHKAI BsiWI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI
BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PsiI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI ZraI
Enzymes that do not cut:
AarI AfeI AflII AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbvCI BcgI BfrBI BglI BmtI
BseRI BsgI BsmI BsmBI BssHII BstEII BstZ17I Bsu36I
ClaI DraIII EcoNI EcoRV FseI FspAI MfeI NdeI
NheI NotI NruI NsiI PacI PmlI PshAI PspOMI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI SwaI XhoI XmnI