Restriction map for
pF1KB5828
(Circular) (Six-base) MAPSORT of: pF1KB5828.seq Check: 7885 from: 1 to: 4553
pF1KB5828 4553 bp
With 182 enzymes: SgfI *
August 1, 2019 16:47 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1210 1210
Size: 4553
AccI GT'mk_AC
Cuts at: 1591 1591
Size: 4553
Acc65I G'GTAC_C
Cuts at: 1569 1569
Size: 4553
AclI AA'CG_TT
Cuts at: 4393 4393
Size: 4553
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 421 2378 2810 3557 3825 421
Size: 1957 432 747 268 1149
Fragments arranged by size:
1957 1149 747 432 268
AfeI AGC'GCT
Cuts at: 719 719
Size: 4553
AflIII A'CryG_T
Cuts at: 1905 3009 3986 1905
Size: 1104 977 2472
Fragments arranged by size:
2472 1104 977
AgeI A'CCGG_T
Cuts at: 2941 2941
Size: 4553
AleI CACnn'nnGTG
Cuts at: 283 283
Size: 4553
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1009 1009
Size: 4553
AlwNI CAG_nnn'CTG
Cuts at: 141 1858 3425 141
Size: 1717 1567 1269
Fragments arranged by size:
1717 1567 1269
ApaI G_GGCC'C
Cuts at: 1145 1145
Size: 4553
ApaLI G'TGCA_C
Cuts at: 418 3323 418
Size: 2905 1648
ApoI r'AATT_y
Cuts at: 1557 3906 4162 1557
Size: 2349 256 1948
Fragments arranged by size:
2349 1948 256
AscI GG'CGCG_CC
Cuts at: 323 323
Size: 4553
AseI AT'TA_AT
Cuts at: 20 20
Size: 4553
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4553
AvaI C'yCGr_G
Cuts at: 180 490 893 1365 1573 180
Size: 310 403 472 208 3160
Fragments arranged by size:
3160 472 403 310 208
BamHI G'GATC_C
Cuts at: 1523 1578 1523
Size: 55 4498
BanI G'GyrC_C
Cuts at: 160 447 656 1155 1569 1983 2231 2266 160
Size: 287 209 499 414 414 248 35 2447
Fragments arranged by size:
2447 499 414 414 287 248 209 35
BanII G_rGCy'C
Cuts at: 388 1003 1084 1145 1258 1567 2597 388
Size: 615 81 61 113 309 1030 2344
Fragments arranged by size:
2344 1030 615 309 113 81 61
BbeI G_GCGC'C
Cuts at: 164 451 660 1159 1987 2235 164
Size: 287 209 499 828 248 2482
Fragments arranged by size:
2482 828 499 287 248 209
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 263 297 263
Size: 34 4519
BciVI GTATCCnnnnn_n'
Cuts at: 2451 3212 4553 2451
Size: 761 1341 2451
Fragments arranged by size:
2451 1341 761
BclI T'GATC_A
Cuts at: 2073 2073
Size: 4553
BglI GCCn_nnn'nGGC
Cuts at: 205 445 1130 1211 1369 205
Size: 240 685 81 158 3389
Fragments arranged by size:
3389 685 240 158 81
BglII A'GATC_T
Cuts at: 1052 2068 4105 1052
Size: 1016 2037 1500
Fragments arranged by size:
2037 1500 1016
BlpI GC'TnA_GC
Cuts at: 997 1665 997
Size: 668 3885
Bme1580I G_kGCm'C
Cuts at: 422 1145 1374 2178 2271 3327 422
Size: 723 229 804 93 1056 1648
Fragments arranged by size:
1648 1056 804 723 229 93
BmrI ACTGGGnnnn_n'
Cuts at: 1765 1846 2181 4325 1765
Size: 81 335 2144 1993
Fragments arranged by size:
2144 1993 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2920 2920
Size: 4553
Bpu10I CC'TnA_GC
Cuts at: 142 142
Size: 4553
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 517 646 916 1729 2608 3100 3398 517
Size: 129 270 813 879 492 298 1672
Fragments arranged by size:
1672 879 813 492 298 270 129
BsaI GGTCTCn'nnnn_
Cuts at: 633 1418 4257 633
Size: 785 2839 929
Fragments arranged by size:
2839 929 785
BsaAI yAC'GTr
Cuts at: 1906 1906
Size: 4553
BsaBI GATnn'nnATC
Cuts at: 6 2067 4104 6
Size: 2061 2037 455
Fragments arranged by size:
2061 2037 455
BsaHI Gr'CG_yC
Cuts at: 161 448 657 1156 1984 2232 2934 4430 161
Size: 287 209 499 828 248 702 1496 284
Fragments arranged by size:
1496 828 702 499 287 284 248 209
BsaWI w'CCGG_w
Cuts at: 1742 2263 2941 3215 3362 3773 1742
Size: 521 678 274 147 411 2522
Fragments arranged by size:
2522 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1129 1159 1258 1288 1129
Size: 30 99 30 4394
Fragments arranged by size:
4394 99 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 784 784
Size: 4553
BseYI C'CCAG_C
Cuts at: 123 249 288 1978 2736 3313 3809 123
Size: 126 39 1690 758 577 496 867
Fragments arranged by size:
1690 867 758 577 496 126 39
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1313 1313
Size: 4553
BsiEI CG_ry'CG
Cuts at: 104 200 235 1355 2095 2141 3349 104
Size: 96 35 1120 740 46 1208 1308
Fragments arranged by size:
1308 1208 1120 740 96 46 35
BsiHKAI G_wGCw'C
Cuts at: 422 523 554 815 1567 2345 2535 3327 422
Size: 101 31 261 752 778 190 792 1648
Fragments arranged by size:
1648 792 778 752 261 190 101 31
BsiWI C'GTAC_G
Cuts at: 314 314
Size: 4553
BsmI GAATG_Cn'
Cuts at: 1240 1240
Size: 4553
Bsp1286I G_dGCh'C
Cuts at: 388 422 523 554 815 1003 1084 1145 1258
Size: 34 101 31 261 188 81 61 113
Cuts at: 1258 1374 1567 2178 2271 2345 2535 2597 3327
Size: 116 193 611 93 74 190 62 730
Cuts at: 3327 388
Size: 1614
Fragments arranged by size:
1614 730 611 261 193 190 188 116
113 101 93 81 74 62 61 34
31
BspEI T'CCGG_A
Cuts at: 3773 3773
Size: 4553
BspHI T'CATG_A
Cuts at: 3729 3729
Size: 4553
BspMI ACCTGCnnnn'nnnn_
Cuts at: 615 1210 1603 1817 2119 2500 615
Size: 595 393 214 302 381 2668
Fragments arranged by size:
2668 595 393 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2845 2899 4383 4548 50
Size: 2795 54 1484 165 55
Fragments arranged by size:
2795 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2465 2465
Size: 4553
BsrFI r'CCGG_y
Cuts at: 450 653 1350 1449 1479 2551 2732 2941 450
Size: 203 697 99 30 1072 181 209 2062
Fragments arranged by size:
2062 1072 697 209 203 181 99 30
BsrGI T'GTAC_A
Cuts at: 1196 1196
Size: 4553
BssHII G'CGCG_C
Cuts at: 323 649 691 1133 323
Size: 326 42 442 3743
Fragments arranged by size:
3743 442 326 42
BssSI C'ACGA_G
Cuts at: 1077 2824 3182 1077
Size: 1747 358 2448
Fragments arranged by size:
2448 1747 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1930 1930
Size: 4553
BstBI TT'CG_AA
Cuts at: 2914 2914
Size: 4553
BstXI CCAn_nnnn'nTGG
Cuts at: 712 712
Size: 4553
BstYI r'GATC_y
Cuts at: 1 360 1052 1523 1578 2045 2068 2403 2649
Size: 359 692 471 55 467 23 335 246
Cuts at: 2649 3661 3747 3759 4105 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 692 471 467 449 359 346 335
246 86 55 23 12
BtgI C'CryG_G
Cuts at: 149 194 242 356 1118 1188 1311 1519 149
Size: 45 48 114 762 70 123 208 3183
Fragments arranged by size:
3183 762 208 123 114 70 48 45
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2503 2684 2847 2503
Size: 181 163 4209
Fragments arranged by size:
4209 181 163
BtsI GCAGTG_nn'
Cuts at: 1796 1796
Size: 4553
DraI TTT'AAA
Cuts at: 1552 1552
Size: 4553
DrdI GACnn_nn'nnGTC
Cuts at: 2259 3117 2259
Size: 858 3695
EaeI y'GGCC_r
Cuts at: 191 197 468 596 2138 2312 2703 2730 4075
Size: 6 271 128 1542 174 391 27 1345
Cuts at: 4075 191
Size: 669
Fragments arranged by size:
1542 1345 669 391 271 174 128 27
6
EagI C'GGCC_G
Cuts at: 197 2138 197
Size: 1941 2612
EarI CTCTTCn'nnn_
Cuts at: 376 727 2576 2786 376
Size: 351 1849 210 2143
Fragments arranged by size:
2143 1849 351 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1081 1137 1533 2761 3071 3217 4363 1081
Size: 56 396 1228 310 146 1146 1271
Fragments arranged by size:
1271 1228 1146 396 310 146 56
EcoICRI GAG'CTC
Cuts at: 1565 1565
Size: 4553
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 421 2378 2810 3557 3825 421
Size: 1957 432 747 268 1149
Fragments arranged by size:
1957 1149 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 622 664 961 1249 622
Size: 42 297 288 3926
Fragments arranged by size:
3926 297 288 42
EcoO109I rG'GnC_Cy
Cuts at: 486 969 1142 1692 486
Size: 483 173 550 3347
Fragments arranged by size:
3347 550 483 173
EcoRI G'AATT_C
Cuts at: 1557 1557
Size: 4553
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1488 2572 1488
Size: 1084 3469
FspI TGC'GCA
Cuts at: 2334 2334
Size: 4553
HaeII r_GCGC'y
Cuts at: 149 164 180 341 451 660 721 1010 1159
Size: 15 16 161 110 209 61 289 149
Cuts at: 1159 1295 1987 2235 3257 4236 149
Size: 136 692 248 1022 979 466
Fragments arranged by size:
1022 979 692 466 289 248 209 161
149 136 110 61 16 15
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 889 921 1375 1407 1415 1447 2395 2425 2427
Size: 32 454 32 8 32 948 30 2
Cuts at: 2427 2457 889
Size: 30 2985
Fragments arranged by size:
2985 948 454 32 32 32 30 30
8 2
HincII GTy'rAC
Cuts at: 1592 1592
Size: 4553
KasI G'GCGC_C
Cuts at: 160 447 656 1155 1983 2231 160
Size: 287 209 499 828 248 2482
Fragments arranged by size:
2482 828 499 287 248 209
KpnI G_GTAC'C
Cuts at: 1573 1573
Size: 4553
MluI A'CGCG_T
Cuts at: 3986 3986
Size: 4553
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1247 3224 3408 1247
Size: 1977 184 2392
Fragments arranged by size:
2392 1977 184
MscI TGG'CCA
Cuts at: 598 2314 598
Size: 1716 2837
MslI CAynn'nnrTG
Cuts at: 283 610 2542 2669 283
Size: 327 1932 127 2167
Fragments arranged by size:
2167 1932 327 127
MspA1I CmG'CkG
Cuts at: 123 151 196 202 244 358 912 1015 1120
Size: 28 45 6 42 114 554 103 105
Cuts at: 1120 1161 1521 1664 1978 2338 3351 3596 123
Size: 41 360 143 314 360 1013 245 1080
Fragments arranged by size:
1080 1013 554 360 360 314 245 143
114 105 103 45 42 41 28 6
NaeI GCC'GGC
Cuts at: 452 655 1451 2734 452
Size: 203 796 1283 2271
Fragments arranged by size:
2271 1283 796 203
NarI GG'CG_CC
Cuts at: 161 448 657 1156 1984 2232 161
Size: 287 209 499 828 248 2482
Fragments arranged by size:
2482 828 499 287 248 209
NcoI C'CATG_G
Cuts at: 1311 1311
Size: 4553
NgoMIV G'CCGG_C
Cuts at: 450 653 1449 2732 450
Size: 203 796 1283 2271
Fragments arranged by size:
2271 1283 796 203
NotI GC'GGCC_GC
Cuts at: 197 197
Size: 4553
NspI r_CATG'y
Cuts at: 297 1272 1606 2106 3013 297
Size: 975 334 500 907 1837
Fragments arranged by size:
1837 975 907 500 334
PasI CC'CwG_GG
Cuts at: 412 412
Size: 4553
PciI A'CATG_T
Cuts at: 3009 3009
Size: 4553
PflMI CCAn_nnn'nTGG
Cuts at: 1055 1055
Size: 4553
PmeI GTTT'AAAC
Cuts at: 1552 1552
Size: 4553
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3692 3724 3692
Size: 32 4521
PpuMI rG'GwC_Cy
Cuts at: 486 486
Size: 4553
PspOMI G'GGCC_C
Cuts at: 1141 1141
Size: 4553
PstI C_TGCA'G
Cuts at: 276 1014 1028 1600 2285 276
Size: 738 14 572 685 2544
Fragments arranged by size:
2544 738 685 572 14
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4553
PvuII CAG'CTG
Cuts at: 123 1978 2338 123
Size: 1855 360 2338
Fragments arranged by size:
2338 1855 360
RsrII CG'GwC_CG
Cuts at: 1395 2748 1395
Size: 1353 3200
SacI G_AGCT'C
Cuts at: 1567 1567
Size: 4553
SacII CC_GC'GG
Cuts at: 152 197 245 359 1121 1522 152
Size: 45 48 114 762 401 3183
Fragments arranged by size:
3183 762 401 114 48 45
SalI G'TCGA_C
Cuts at: 1590 1590
Size: 4553
SanDI GG'GwC_CC
Cuts at: 486 486
Size: 4553
SapI GCTCTTCn'nnn_
Cuts at: 727 2576 2786 727
Size: 1849 210 2494
Fragments arranged by size:
2494 1849 210
SbfI CC_TGCA'GG
Cuts at: 1028 1600 1028
Size: 572 3981
ScaI AGT'ACT
Cuts at: 4070 4070
Size: 4553
SexAI A'CCwGG_T
Cuts at: 634 634
Size: 4553
SfcI C'TryA_G
Cuts at: 33 272 333 1010 1024 1596 2281 3274 3465
Size: 239 61 677 14 572 685 993 191
Cuts at: 3465 33
Size: 1121
Fragments arranged by size:
1121 993 685 677 572 239 191 61
14
SfoI GGC'GCC
Cuts at: 162 449 658 1157 1985 2233 162
Size: 287 209 499 828 248 2482
Fragments arranged by size:
2482 828 499 287 248 209
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4553
SgrAI Cr'CCGG_yG
Cuts at: 653 653
Size: 4553
SmaI CCC'GGG
Cuts at: 182 492 895 1367 1575 182
Size: 310 403 472 208 3160
Fragments arranged by size:
3160 472 403 310 208
SmlI C'TyrA_G
Cuts at: 532 661 931 1708 2623 3115 3377 532
Size: 129 270 777 915 492 262 1708
Fragments arranged by size:
1708 915 777 492 270 262 129
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4553
SphI G_CATG'C
Cuts at: 1606 2106 1606
Size: 500 4053
StuI AGG'CCT
Cuts at: 538 1390 538
Size: 852 3701
StyI C'CwwG_G
Cuts at: 575 900 950 1097 1311 1687 2041 575
Size: 325 50 147 214 376 354 3087
Fragments arranged by size:
3087 376 354 325 214 147 50
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1383 2136 2269 2728 2937 1383
Size: 753 133 459 209 2999
Fragments arranged by size:
2999 753 459 209 133
TatI w'GTAC_w
Cuts at: 1196 4068 1196
Size: 2872 1681
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1129 1161 4070 4102 1129
Size: 32 2909 32 1580
Fragments arranged by size:
2909 1580 32 32
Tth111I GACn'n_nGTC
Cuts at: 1429 2350 1429
Size: 921 3632
XbaI T'CTAG_A
Cuts at: 1584 1584
Size: 4553
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2048 2048
Size: 4553
XmaI C'CCGG_G
Cuts at: 180 490 893 1365 1573 180
Size: 310 403 472 208 3160
Fragments arranged by size:
3160 472 403 310 208
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AleI AlfI AlwNI ApaI ApaLI ApoI AscI AseI
AsiSI AvaI BamHI BanI BanII BbeI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmrI BplI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsiWI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NotI NspI PasI PciI PflMI PmeI
PpiI PpuMI PspOMI PstI PvuI PvuII RsrII SacI
SacII SalI SanDI SapI SbfI ScaI SexAI SfcI
SfoI SgfI SgrAI SmaI SmlI SpeI SphI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AflII AhdI AloI AvrII BaeI BbsI BbvCI
BfrBI BmgBI BmtI BpmI BsmBI BstEII BstZ17I Bsu36I
ClaI CspCI DraIII EcoRV FseI FspAI HindIII HpaI
MfeI NdeI NheI NruI NsiI PacI PfoI PmlI
PshAI PsiI PspXI PsrI SfiI SnaBI SrfI SspI
SwaI XhoI XmnI ZraI