Restriction map for
pF1KB5827
(Circular) (Six-base) MAPSORT of: pF1KB5827.seq Check: 3199 from: 1 to: 5180
pF1KB5827 5180 bp
With 182 enzymes: SgfI *
September 25, 2012 15:34 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1775 1775
Size: 5180
AccI GT'mk_AC
Cuts at: 2218 2218
Size: 5180
Acc65I G'GTAC_C
Cuts at: 2196 2196
Size: 5180
AclI AA'CG_TT
Cuts at: 5020 5020
Size: 5180
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 3005 3437 4184 4452 3005
Size: 432 747 268 3733
Fragments arranged by size:
3733 747 432 268
AflIII A'CryG_T
Cuts at: 2532 3636 4613 2532
Size: 1104 977 3099
Fragments arranged by size:
3099 1104 977
AgeI A'CCGG_T
Cuts at: 3568 3568
Size: 5180
AhdI GACnn_n'nnGTC
Cuts at: 1611 1611
Size: 5180
AleI CACnn'nnGTG
Cuts at: 1777 1777
Size: 5180
AlwNI CAG_nnn'CTG
Cuts at: 1313 1379 1455 2485 4052 1313
Size: 66 76 1030 1567 2441
Fragments arranged by size:
2441 1567 1030 76 66
ApaI G_GGCC'C
Cuts at: 891 2025 891
Size: 1134 4046
ApaLI G'TGCA_C
Cuts at: 3950 3950
Size: 5180
ApoI r'AATT_y
Cuts at: 2184 4533 4789 2184
Size: 2349 256 2575
Fragments arranged by size:
2575 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5180
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5180
AvaI C'yCGr_G
Cuts at: 220 2200 220
Size: 1980 3200
AvrII C'CTAG_G
Cuts at: 819 819
Size: 5180
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1393 1426 1393
Size: 33 5147
BamHI G'GATC_C
Cuts at: 2205 2205
Size: 5180
BanI G'GyrC_C
Cuts at: 175 181 1994 2196 2610 2858 2893 175
Size: 6 1813 202 414 248 35 2462
Fragments arranged by size:
2462 1813 414 248 202 35 6
BanII G_rGCy'C
Cuts at: 692 891 1262 1762 1809 2025 2194 3224 692
Size: 199 371 500 47 216 169 1030 2648
Fragments arranged by size:
2648 1030 500 371 216 199 169 47
BbeI G_GCGC'C
Cuts at: 179 185 2614 2862 179
Size: 6 2429 248 2497
Fragments arranged by size:
2497 2429 248 6
BbsI GAAGACnn'nnnn_
Cuts at: 1572 2083 1572
Size: 511 4669
BbvCI CC'TCA_GC
Cuts at: 841 1459 841
Size: 618 4562
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 201 235 201
Size: 34 5146
BciVI GTATCCnnnnn_n'
Cuts at: 784 3078 3839 5180 784
Size: 2294 761 1341 784
Fragments arranged by size:
2294 1341 784 761
BclI T'GATC_A
Cuts at: 2700 2700
Size: 5180
BfrBI ATG'CAT
Cuts at: 1020 1020
Size: 5180
BglI GCCn_nnn'nGGC
Cuts at: 197 1992 197
Size: 1795 3385
BglII A'GATC_T
Cuts at: 2695 4732 2695
Size: 2037 3143
BlpI GC'TnA_GC
Cuts at: 592 1372 2292 592
Size: 780 920 3480
Fragments arranged by size:
3480 920 780
Bme1580I G_kGCm'C
Cuts at: 891 2025 2154 2805 2898 3954 891
Size: 1134 129 651 93 1056 2117
Fragments arranged by size:
2117 1134 1056 651 129 93
BmrI ACTGGGnnnn_n'
Cuts at: 2157 2392 2473 2808 4952 2157
Size: 235 81 335 2144 2385
Fragments arranged by size:
2385 2144 335 235 81
BmtI G_CTAG'C
Cuts at: 1682 1682
Size: 5180
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3547 3547
Size: 5180
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 460 706 1335 1381 460
Size: 246 629 46 4259
Fragments arranged by size:
4259 629 246 46
Bpu10I CC'TnA_GC
Cuts at: 841 1264 1331 1459 841
Size: 423 67 128 4562
Fragments arranged by size:
4562 423 128 67
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1420 2026 2356 3235 3727 4025 1420
Size: 606 330 879 492 298 2575
Fragments arranged by size:
2575 879 606 492 330 298
BsaI GGTCTCn'nnnn_
Cuts at: 686 4884 686
Size: 4198 982
BsaAI yAC'GTr
Cuts at: 643 2533 643
Size: 1890 3290
BsaBI GATnn'nnATC
Cuts at: 6 2694 4731 6
Size: 2688 2037 455
Fragments arranged by size:
2688 2037 455
BsaHI Gr'CG_yC
Cuts at: 176 182 2611 2859 3561 5057 176
Size: 6 2429 248 702 1496 299
Fragments arranged by size:
2429 1496 702 299 248 6
BsaWI w'CCGG_w
Cuts at: 1610 2369 2890 3568 3842 3989 4400 1610
Size: 759 521 678 274 147 411 2390
Fragments arranged by size:
2390 759 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1117 1147 1117
Size: 30 5150
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 209 389 563 1552 209
Size: 180 174 989 3837
Fragments arranged by size:
3837 989 180 174
BseYI C'CCAG_C
Cuts at: 939 960 1114 1734 1786 2168 2605 3363 3940
Size: 21 154 620 52 382 437 758 577
Cuts at: 3940 4436 939
Size: 496 1683
Fragments arranged by size:
1683 758 620 577 496 437 382 154
52 21
BsiEI CG_ry'CG
Cuts at: 104 192 2722 2768 3976 104
Size: 88 2530 46 1208 1308
Fragments arranged by size:
2530 1308 1208 88 46
BsiHKAI G_wGCw'C
Cuts at: 692 1262 1367 2033 2194 2972 3162 3954 692
Size: 570 105 666 161 778 190 792 1918
Fragments arranged by size:
1918 792 778 666 570 190 161 105
Bsp1286I G_dGCh'C
Cuts at: 692 891 1262 1367 1762 1809 2025 2033 2154
Size: 199 371 105 395 47 216 8 121
Cuts at: 2154 2194 2805 2898 2972 3162 3224 3954 692
Size: 40 611 93 74 190 62 730 1918
Fragments arranged by size:
1918 730 611 395 371 216 199 190
121 105 93 74 62 47 40 8
BspEI T'CCGG_A
Cuts at: 4400 4400
Size: 5180
BspHI T'CATG_A
Cuts at: 308 606 4356 308
Size: 298 3750 1132
Fragments arranged by size:
3750 1132 298
BspMI ACCTGCnnnn'nnnn_
Cuts at: 321 482 892 1518 1683 1775 2163 2230 2444
Size: 161 410 626 165 92 388 67 214
Cuts at: 2444 2746 3127 321
Size: 302 381 2374
Fragments arranged by size:
2374 626 410 388 381 302 214 165
161 92 67
BsrBI CCG'CTC
Cuts at: 50 291 1752 3472 3526 5010 5175 50
Size: 241 1461 1720 54 1484 165 55
Fragments arranged by size:
1720 1484 1461 241 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3092 3092
Size: 5180
BsrFI r'CCGG_y
Cuts at: 178 184 1646 3178 3359 3568 178
Size: 6 1462 1532 181 209 1790
Fragments arranged by size:
1790 1532 1462 209 181 6
BssSI C'ACGA_G
Cuts at: 3451 3809 3451
Size: 358 4822
BstAPI GCAn_nnn'nTGC
Cuts at: 907 2001 2557 907
Size: 1094 556 3530
Fragments arranged by size:
3530 1094 556
BstBI TT'CG_AA
Cuts at: 3541 3541
Size: 5180
BstEII G'GTnAC_C
Cuts at: 760 2058 760
Size: 1298 3882
BstXI CCAn_nnnn'nTGG
Cuts at: 527 527
Size: 5180
BstYI r'GATC_y
Cuts at: 1 1904 2205 2672 2695 3030 3276 4288 4374
Size: 1903 301 467 23 335 246 1012 86
Cuts at: 4374 4386 4732 1
Size: 12 346 449
Fragments arranged by size:
1903 1012 467 449 346 335 301 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 809 1775 107
Size: 702 966 3512
Fragments arranged by size:
3512 966 702
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3130 3311 3474 3130
Size: 181 163 4836
Fragments arranged by size:
4836 181 163
BtsI GCAGTG_nn'
Cuts at: 1744 2031 2423 1744
Size: 287 392 4501
Fragments arranged by size:
4501 392 287
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 371 406 371
Size: 35 5145
DraI TTT'AAA
Cuts at: 2179 2179
Size: 5180
DrdI GACnn_nn'nnGTC
Cuts at: 2886 3744 2886
Size: 858 4322
EaeI y'GGCC_r
Cuts at: 189 542 1644 2765 2939 3330 3357 4702 189
Size: 353 1102 1121 174 391 27 1345 667
Fragments arranged by size:
1345 1121 1102 667 391 353 174 27
EagI C'GGCC_G
Cuts at: 189 2765 189
Size: 2576 2604
EarI CTCTTCn'nnn_
Cuts at: 3203 3413 3203
Size: 210 4970
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3388 3698 3844 4990 3388
Size: 310 146 1146 3578
Fragments arranged by size:
3578 1146 310 146
EcoICRI GAG'CTC
Cuts at: 690 1260 2192 690
Size: 570 932 3678
Fragments arranged by size:
3678 932 570
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 460 706 1335 1381 3005 3437 4184 4452 460
Size: 246 629 46 1624 432 747 268 1188
Fragments arranged by size:
1624 1188 747 629 432 268 246 46
EcoNI CCTnn'n_nnAGG
Cuts at: 478 919 1033 478
Size: 441 114 4625
Fragments arranged by size:
4625 441 114
EcoO109I rG'GnC_Cy
Cuts at: 333 537 845 888 1515 1537 2010 2319 333
Size: 204 308 43 627 22 473 309 3194
Fragments arranged by size:
3194 627 473 309 308 204 43 22
EcoRI G'AATT_C
Cuts at: 2184 2184
Size: 5180
EcoRV GAT'ATC
Cuts at: 2165 2165
Size: 5180
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1083 3199 1083
Size: 2116 3064
FspI TGC'GCA
Cuts at: 2961 2961
Size: 5180
HaeII r_GCGC'y
Cuts at: 179 185 220 2614 2862 3884 4863 179
Size: 6 35 2394 248 1022 979 496
Fragments arranged by size:
2394 1022 979 496 248 35 6
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1597 1629 3022 3052 3054 3084 1597
Size: 32 1393 30 2 30 3693
Fragments arranged by size:
3693 1393 32 30 30 2
HincII GTy'rAC
Cuts at: 2219 2219
Size: 5180
HindIII A'AGCT_T
Cuts at: 1438 1438
Size: 5180
KasI G'GCGC_C
Cuts at: 175 181 2610 2858 175
Size: 6 2429 248 2497
Fragments arranged by size:
2497 2429 248 6
KpnI G_GTAC'C
Cuts at: 2200 2200
Size: 5180
MluI A'CGCG_T
Cuts at: 4613 4613
Size: 5180
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3851 4035 3851
Size: 184 4996
MscI TGG'CCA
Cuts at: 544 2941 544
Size: 2397 2783
MslI CAynn'nnrTG
Cuts at: 525 605 880 1777 1814 1973 2036 2131 3169
Size: 80 275 897 37 159 63 95 1038
Cuts at: 3169 3296 525
Size: 127 2409
Fragments arranged by size:
2409 1038 897 275 159 127 95 80
63 37
MspA1I CmG'CkG
Cuts at: 1131 1323 1730 1797 2291 2605 2965 3978 4223
Size: 192 407 67 494 314 360 1013 245
Cuts at: 4223 1131
Size: 2088
Fragments arranged by size:
2088 1013 494 407 360 314 245 192
67
NaeI GCC'GGC
Cuts at: 180 186 3361 180
Size: 6 3175 1999
Fragments arranged by size:
3175 1999 6
NarI GG'CG_CC
Cuts at: 176 182 2611 2859 176
Size: 6 2429 248 2497
Fragments arranged by size:
2497 2429 248 6
NcoI C'CATG_G
Cuts at: 107 107
Size: 5180
NdeI CA'TA_TG
Cuts at: 526 526
Size: 5180
NgoMIV G'CCGG_C
Cuts at: 178 184 3359 178
Size: 6 3175 1999
Fragments arranged by size:
3175 1999 6
NheI G'CTAG_C
Cuts at: 1678 1678
Size: 5180
NsiI A_TGCA'T
Cuts at: 1022 1022
Size: 5180
NspI r_CATG'y
Cuts at: 2048 2233 2733 3640 2048
Size: 185 500 907 3588
Fragments arranged by size:
3588 907 500 185
PasI CC'CwG_GG
Cuts at: 891 891
Size: 5180
PciI A'CATG_T
Cuts at: 3636 3636
Size: 5180
PflMI CCAn_nnn'nTGG
Cuts at: 884 1313 884
Size: 429 4751
PfoI T'CCnGG_A
Cuts at: 830 830
Size: 5180
PmeI GTTT'AAAC
Cuts at: 2179 2179
Size: 5180
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4319 4351 4319
Size: 32 5148
PpuMI rG'GwC_Cy
Cuts at: 333 537 845 1537 333
Size: 204 308 692 3976
Fragments arranged by size:
3976 692 308 204
PshAI GACnn'nnGTC
Cuts at: 704 704
Size: 5180
PspOMI G'GGCC_C
Cuts at: 887 2021 887
Size: 1134 4046
PstI C_TGCA'G
Cuts at: 479 1124 2227 2912 479
Size: 645 1103 685 2747
Fragments arranged by size:
2747 1103 685 645
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5180
PvuII CAG'CTG
Cuts at: 1131 1323 1730 1797 2605 2965 1131
Size: 192 407 67 808 360 3346
Fragments arranged by size:
3346 808 407 360 192 67
RsrII CG'GwC_CG
Cuts at: 3375 3375
Size: 5180
SacI G_AGCT'C
Cuts at: 692 1262 2194 692
Size: 570 932 3678
Fragments arranged by size:
3678 932 570
SalI G'TCGA_C
Cuts at: 2217 2217
Size: 5180
SapI GCTCTTCn'nnn_
Cuts at: 3203 3413 3203
Size: 210 4970
SbfI CC_TGCA'GG
Cuts at: 1124 2227 1124
Size: 1103 4077
ScaI AGT'ACT
Cuts at: 4697 4697
Size: 5180
SfcI C'TryA_G
Cuts at: 33 475 618 1120 1586 1710 1725 1900 2119
Size: 442 143 502 466 124 15 175 219
Cuts at: 2119 2223 2908 3901 4092 33
Size: 104 685 993 191 1121
Fragments arranged by size:
1121 993 685 502 466 442 219 191
175 143 124 104 15
SfoI GGC'GCC
Cuts at: 177 183 2612 2860 177
Size: 6 2429 248 2497
Fragments arranged by size:
2497 2429 248 6
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5180
SgrAI Cr'CCGG_yG
Cuts at: 178 184 178
Size: 6 5174
SmaI CCC'GGG
Cuts at: 2202 2202
Size: 5180
SmlI C'TyrA_G
Cuts at: 1435 2005 2335 3250 3742 4004 1435
Size: 570 330 915 492 262 2611
Fragments arranged by size:
2611 915 570 492 330 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5180
SphI G_CATG'C
Cuts at: 2048 2233 2733 2048
Size: 185 500 4495
Fragments arranged by size:
4495 500 185
StuI AGG'CCT
Cuts at: 1633 1633
Size: 5180
StyI C'CwwG_G
Cuts at: 107 819 1034 1058 2314 2668 107
Size: 712 215 24 1256 354 2619
Fragments arranged by size:
2619 1256 712 354 215 24
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1128 2763 2896 3355 3564 1128
Size: 1635 133 459 209 2744
Fragments arranged by size:
2744 1635 459 209 133
TatI w'GTAC_w
Cuts at: 4695 4695
Size: 5180
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 917 949 1117 1149 4697 4729 917
Size: 32 168 32 3548 32 1368
Fragments arranged by size:
3548 1368 168 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2977 2977
Size: 5180
XbaI T'CTAG_A
Cuts at: 2211 2211
Size: 5180
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 996 1356 1794 2675 996
Size: 360 438 881 3501
Fragments arranged by size:
3501 881 438 360
XmaI C'CCGG_G
Cuts at: 2200 2200
Size: 5180
XmnI GAAnn'nnTTC
Cuts at: 1424 1424
Size: 5180
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AhdI
AleI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
AvrII BaeI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII HindIII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI
NsiI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PspOMI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI
SmaI SmlI SpeI SphI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AlfI AloI AscI BmgBI BsgI
BsiWI BsmI BsmBI BsrGI BssHII BstZ17I Bsu36I ClaI
DraIII FseI FspAI HpaI MfeI NotI NruI PacI
PmlI PsiI PspXI PsrI SacII SanDI SexAI SfiI
SnaBI SrfI SspI SwaI XhoI ZraI