Restriction map for
pF1KB5824
(Circular) (Six-base) MAPSORT of: pF1KB5824.seq Check: 4231 from: 1 to: 4541
pF1KB5824 4541 bp
With 182 enzymes: SgfI *
October 7, 2009 14:17 ..
AccI GT'mk_AC
Cuts at: 245 1579 245
Size: 1334 3207
Acc65I G'GTAC_C
Cuts at: 1557 1557
Size: 4541
AclI AA'CG_TT
Cuts at: 4381 4381
Size: 4541
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 221 664 929 1495 2366 2798 3545 3813 221
Size: 443 265 566 871 432 747 268 949
Fragments arranged by size:
949 871 747 566 443 432 268 265
AflIII A'CryG_T
Cuts at: 1150 1893 2997 3974 1150
Size: 743 1104 977 1717
Fragments arranged by size:
1717 1104 977 743
AgeI A'CCGG_T
Cuts at: 2929 2929
Size: 4541
AlwNI CAG_nnn'CTG
Cuts at: 1846 3413 1846
Size: 1567 2974
ApaLI G'TGCA_C
Cuts at: 3311 3311
Size: 4541
ApoI r'AATT_y
Cuts at: 313 655 1392 1545 3894 4150 313
Size: 342 737 153 2349 256 704
Fragments arranged by size:
2349 737 704 342 256 153
AseI AT'TA_AT
Cuts at: 20 20
Size: 4541
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4541
AvaI C'yCGr_G
Cuts at: 935 1561 935
Size: 626 3915
BamHI G'GATC_C
Cuts at: 1198 1566 1198
Size: 368 4173
BanI G'GyrC_C
Cuts at: 160 1189 1557 1971 2219 2254 160
Size: 1029 368 414 248 35 2447
Fragments arranged by size:
2447 1029 414 368 248 35
BanII G_rGCy'C
Cuts at: 1555 2585 1555
Size: 1030 3511
BbeI G_GCGC'C
Cuts at: 1975 2223 1975
Size: 248 4293
BbsI GAAGACnn'nnnn_
Cuts at: 134 134
Size: 4541
BciVI GTATCCnnnnn_n'
Cuts at: 1086 2439 3200 4541 1086
Size: 1353 761 1341 1086
Fragments arranged by size:
1353 1341 1086 761
BclI T'GATC_A
Cuts at: 1158 2061 1158
Size: 903 3638
BglII A'GATC_T
Cuts at: 272 2056 4093 272
Size: 1784 2037 720
Fragments arranged by size:
2037 1784 720
BlpI GC'TnA_GC
Cuts at: 1653 1653
Size: 4541
Bme1580I G_kGCm'C
Cuts at: 119 2166 2259 3315 119
Size: 2047 93 1056 1345
Fragments arranged by size:
2047 1345 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 141 734 962 1753 1834 2169 4313 141
Size: 593 228 791 81 335 2144 369
Fragments arranged by size:
2144 791 593 369 335 228 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2908 2908
Size: 4541
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 379 617 737 995 1035 379
Size: 238 120 258 40 3885
Fragments arranged by size:
3885 258 238 120 40
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 898 1717 2596 3088 3386 898
Size: 819 879 492 298 2053
Fragments arranged by size:
2053 879 819 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4245 4245
Size: 4541
BsaAI yAC'GTr
Cuts at: 1894 1894
Size: 4541
BsaBI GATnn'nnATC
Cuts at: 6 277 2055 4092 6
Size: 271 1778 2037 455
Fragments arranged by size:
2037 1778 455 271
BsaHI Gr'CG_yC
Cuts at: 1972 2220 2922 4418 1972
Size: 248 702 1496 2095
Fragments arranged by size:
2095 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1730 2251 2929 3203 3350 3761 1730
Size: 521 678 274 147 411 2510
Fragments arranged by size:
2510 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 389 809 389
Size: 420 4121
BseYI C'CCAG_C
Cuts at: 1142 1966 2724 3301 3797 1142
Size: 824 758 577 496 1886
Fragments arranged by size:
1886 824 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 2083 2129 3337 104
Size: 1979 46 1208 1308
Fragments arranged by size:
1979 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1555 2333 2523 3315 1555
Size: 778 190 792 2781
Fragments arranged by size:
2781 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 119 1555 2166 2259 2333 2523 2585 3315 119
Size: 1436 611 93 74 190 62 730 1345
Fragments arranged by size:
1436 1345 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3761 3761
Size: 4541
BspHI T'CATG_A
Cuts at: 297 3717 297
Size: 3420 1121
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1591 1805 2107 2488 1591
Size: 214 302 381 3644
Fragments arranged by size:
3644 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2833 2887 4371 4536 50
Size: 2783 54 1484 165 55
Fragments arranged by size:
2783 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 713 2453 713
Size: 1740 2801
BsrFI r'CCGG_y
Cuts at: 2539 2720 2929 2539
Size: 181 209 4151
Fragments arranged by size:
4151 209 181
BssSI C'ACGA_G
Cuts at: 2812 3170 2812
Size: 358 4183
BstAPI GCAn_nnn'nTGC
Cuts at: 1918 1918
Size: 4541
BstBI TT'CG_AA
Cuts at: 2902 2902
Size: 4541
BstYI r'GATC_y
Cuts at: 1 272 1198 1566 2033 2056 2391 2637 3649
Size: 271 926 368 467 23 335 246 1012
Cuts at: 3649 3735 3747 4093 1
Size: 86 12 346 449
Fragments arranged by size:
1012 926 467 449 368 346 335 271
246 86 23 12
BtgI C'CryG_G
Cuts at: 107 727 107
Size: 620 3921
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2491 2672 2835 2491
Size: 181 163 4197
Fragments arranged by size:
4197 181 163
BtsI GCAGTG_nn'
Cuts at: 119 221 1784 119
Size: 102 1563 2876
Fragments arranged by size:
2876 1563 102
ClaI AT'CG_AT
Cuts at: 809 809
Size: 4541
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 591 626 591
Size: 35 4506
DraI TTT'AAA
Cuts at: 1540 1540
Size: 4541
DrdI GACnn_nn'nnGTC
Cuts at: 2247 3105 2247
Size: 858 3683
EaeI y'GGCC_r
Cuts at: 2126 2300 2691 2718 4063 2126
Size: 174 391 27 1345 2604
Fragments arranged by size:
2604 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2126 2126
Size: 4541
EarI CTCTTCn'nnn_
Cuts at: 640 645 2564 2774 640
Size: 5 1919 210 2407
Fragments arranged by size:
2407 1919 210 5
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2749 3059 3205 4351 2749
Size: 310 146 1146 2939
Fragments arranged by size:
2939 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1553 1553
Size: 4541
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 221 379 617 664 737 929 995 1035 1495
Size: 158 238 47 73 192 66 40 460
Cuts at: 1495 2366 2798 3545 3813 221
Size: 871 432 747 268 949
Fragments arranged by size:
949 871 747 460 432 268 238 192
158 73 66 47 40
EcoO109I rG'GnC_Cy
Cuts at: 932 1680 932
Size: 748 3793
EcoRI G'AATT_C
Cuts at: 1545 1545
Size: 4541
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 196 1485 2560 196
Size: 1289 1075 2177
Fragments arranged by size:
2177 1289 1075
FspI TGC'GCA
Cuts at: 2322 2322
Size: 4541
HaeII r_GCGC'y
Cuts at: 1975 2223 3245 4224 1975
Size: 248 1022 979 2292
Fragments arranged by size:
2292 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 667 699 841 873 2383 2413 2415 2445 667
Size: 32 142 32 1510 30 2 30 2763
Fragments arranged by size:
2763 1510 142 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1425 1580 1425
Size: 155 4386
KasI G'GCGC_C
Cuts at: 1971 2219 1971
Size: 248 4293
KpnI G_GTAC'C
Cuts at: 1561 1561
Size: 4541
MluI A'CGCG_T
Cuts at: 3974 3974
Size: 4541
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 500 3212 3396 500
Size: 2712 184 1645
Fragments arranged by size:
2712 1645 184
MscI TGG'CCA
Cuts at: 2302 2302
Size: 4541
MslI CAynn'nnrTG
Cuts at: 112 302 409 2530 2657 112
Size: 190 107 2121 127 1996
Fragments arranged by size:
2121 1996 190 127 107
MspA1I CmG'CkG
Cuts at: 749 1221 1652 1966 2326 3339 3584 749
Size: 472 431 314 360 1013 245 1706
Fragments arranged by size:
1706 1013 472 431 360 314 245
NaeI GCC'GGC
Cuts at: 2722 2722
Size: 4541
NarI GG'CG_CC
Cuts at: 1972 2220 1972
Size: 248 4293
NcoI C'CATG_G
Cuts at: 107 727 107
Size: 620 3921
NdeI CA'TA_TG
Cuts at: 624 1163 624
Size: 539 4002
NgoMIV G'CCGG_C
Cuts at: 2720 2720
Size: 4541
NspI r_CATG'y
Cuts at: 414 1154 1594 2094 3001 414
Size: 740 440 500 907 1954
Fragments arranged by size:
1954 907 740 500 440
PasI CC'CwG_GG
Cuts at: 150 150
Size: 4541
PciI A'CATG_T
Cuts at: 1150 2997 1150
Size: 1847 2694
PmeI GTTT'AAAC
Cuts at: 1540 1540
Size: 4541
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3680 3712 3680
Size: 32 4509
PshAI GACnn'nnGTC
Cuts at: 749 749
Size: 4541
PstI C_TGCA'G
Cuts at: 1588 2273 1588
Size: 685 3856
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4541
PvuII CAG'CTG
Cuts at: 749 1966 2326 749
Size: 1217 360 2964
Fragments arranged by size:
2964 1217 360
RsrII CG'GwC_CG
Cuts at: 2736 2736
Size: 4541
SacI G_AGCT'C
Cuts at: 1555 1555
Size: 4541
SalI G'TCGA_C
Cuts at: 1578 1578
Size: 4541
SapI GCTCTTCn'nnn_
Cuts at: 2564 2774 2564
Size: 210 4331
SbfI CC_TGCA'GG
Cuts at: 1588 1588
Size: 4541
ScaI AGT'ACT
Cuts at: 364 698 1048 4058 364
Size: 334 350 3010 847
Fragments arranged by size:
3010 847 350 334
SfcI C'TryA_G
Cuts at: 33 366 1584 2269 3262 3453 33
Size: 333 1218 685 993 191 1121
Fragments arranged by size:
1218 1121 993 685 333 191
SfoI GGC'GCC
Cuts at: 1973 2221 1973
Size: 248 4293
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4541
SmaI CCC'GGG
Cuts at: 1563 1563
Size: 4541
SmlI C'TyrA_G
Cuts at: 913 1696 2611 3103 3365 913
Size: 783 915 492 262 2089
Fragments arranged by size:
2089 915 783 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4541
SphI G_CATG'C
Cuts at: 414 1594 2094 414
Size: 1180 500 2861
Fragments arranged by size:
2861 1180 500
SspI AAT'ATT
Cuts at: 261 349 1418 261
Size: 88 1069 3384
Fragments arranged by size:
3384 1069 88
StyI C'CwwG_G
Cuts at: 107 727 796 1675 2029 107
Size: 620 69 879 354 2619
Fragments arranged by size:
2619 879 620 354 69
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2124 2257 2716 2925 2124
Size: 133 459 209 3740
Fragments arranged by size:
3740 459 209 133
TatI w'GTAC_w
Cuts at: 362 696 1046 4056 362
Size: 334 350 3010 847
Fragments arranged by size:
3010 847 350 334
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 976 1008 4058 4090 976
Size: 32 3050 32 1427
Fragments arranged by size:
3050 1427 32 32
Tth111I GACn'n_nGTC
Cuts at: 2338 2338
Size: 4541
XbaI T'CTAG_A
Cuts at: 1572 1572
Size: 4541
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 677 2036 677
Size: 1359 3182
XmaI C'CCGG_G
Cuts at: 1561 1561
Size: 4541
XmnI GAAnn'nnTTC
Cuts at: 512 512
Size: 4541
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BciVI BclI BglII BlpI Bme1580I BmrI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI
BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NspI PasI
PciI PmeI PpiI PshAI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI SspI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbvCI BcgI BfrBI BglI
BmgBI BmtI Bpu10I BsaXI BsgI BsiWI BsmI BsmBI
BsrGI BssHII BstEII BstXI BstZ17I Bsu36I DraIII EcoNI
EcoRV FseI FspAI HindIII HpaI MfeI NheI NotI
NruI NsiI PacI PflMI PfoI PmlI PpuMI PsiI
PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI StuI SwaI XhoI ZraI