Restriction map for
pF1KB5816
(Circular) (Six-base) MAPSORT of: pF1KB5816.seq Check: 1175 from: 1 to: 5738
pF1KB5816 5738 bp
With 182 enzymes: SgfI *
March 2, 2010 14:26 ..
AatII G_ACGT'C
Cuts at: 207 467 207
Size: 260 5478
AccI GT'mk_AC
Cuts at: 2776 2776
Size: 5738
Acc65I G'GTAC_C
Cuts at: 1433 2754 1433
Size: 1321 4417
AclI AA'CG_TT
Cuts at: 5578 5578
Size: 5738
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 3563 3995 4742 5010 3563
Size: 432 747 268 4291
Fragments arranged by size:
4291 747 432 268
AfeI AGC'GCT
Cuts at: 301 619 1271 301
Size: 318 652 4768
Fragments arranged by size:
4768 652 318
AflIII A'CryG_T
Cuts at: 3090 4194 5171 3090
Size: 1104 977 3657
Fragments arranged by size:
3657 1104 977
AgeI A'CCGG_T
Cuts at: 4126 4126
Size: 5738
AhdI GACnn_n'nnGTC
Cuts at: 772 772
Size: 5738
AleI CACnn'nnGTG
Cuts at: 1178 1178
Size: 5738
AlwNI CAG_nnn'CTG
Cuts at: 1216 2226 2487 3043 4610 1216
Size: 1010 261 556 1567 2344
Fragments arranged by size:
2344 1567 1010 556 261
ApaI G_GGCC'C
Cuts at: 427 756 2048 427
Size: 329 1292 4117
Fragments arranged by size:
4117 1292 329
ApaLI G'TGCA_C
Cuts at: 4508 4508
Size: 5738
ApoI r'AATT_y
Cuts at: 2266 2742 5091 5347 2266
Size: 476 2349 256 2657
Fragments arranged by size:
2657 2349 476 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5738
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5738
AvaI C'yCGr_G
Cuts at: 746 1403 2758 746
Size: 657 1355 3726
Fragments arranged by size:
3726 1355 657
AvrII C'CTAG_G
Cuts at: 990 990
Size: 5738
BamHI G'GATC_C
Cuts at: 2763 2763
Size: 5738
BanI G'GyrC_C
Cuts at: 1433 2754 3168 3416 3451 1433
Size: 1321 414 248 35 3720
Fragments arranged by size:
3720 1321 414 248 35
BanII G_rGCy'C
Cuts at: 427 756 956 1469 1585 2048 2752 3782 427
Size: 329 200 513 116 463 704 1030 2383
Fragments arranged by size:
2383 1030 704 513 463 329 200 116
BbeI G_GCGC'C
Cuts at: 3172 3420 3172
Size: 248 5490
BbsI GAAGACnn'nnnn_
Cuts at: 562 768 1043 1130 2101 562
Size: 206 275 87 971 4199
Fragments arranged by size:
4199 971 275 206 87
BbvCI CC'TCA_GC
Cuts at: 443 1244 443
Size: 801 4937
BciVI GTATCCnnnnn_n'
Cuts at: 3636 4397 5738 3636
Size: 761 1341 3636
Fragments arranged by size:
3636 1341 761
BclI T'GATC_A
Cuts at: 3258 3258
Size: 5738
BglI GCCn_nnn'nGGC
Cuts at: 751 751
Size: 5738
BglII A'GATC_T
Cuts at: 1535 3253 5290 1535
Size: 1718 2037 1983
Fragments arranged by size:
2037 1983 1718
BlpI GC'TnA_GC
Cuts at: 1579 2375 2850 1579
Size: 796 475 4467
Fragments arranged by size:
4467 796 475
Bme1580I G_kGCm'C
Cuts at: 427 756 2048 3363 3456 4512 427
Size: 329 1292 1315 93 1056 1653
Fragments arranged by size:
1653 1315 1292 1056 329 93
BmrI ACTGGGnnnn_n'
Cuts at: 366 2236 2950 3031 3366 5510 366
Size: 1870 714 81 335 2144 594
Fragments arranged by size:
2144 1870 714 594 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 321 387 1434 2160 4105 321
Size: 66 1047 726 1945 1954
Fragments arranged by size:
1954 1945 1047 726 66
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 931 1001 1354 2104 931
Size: 70 353 750 4565
Fragments arranged by size:
4565 750 353 70
Bpu10I CC'TnA_GC
Cuts at: 443 1244 443
Size: 801 4937
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1258 2914 3793 4285 4583 1258
Size: 1656 879 492 298 2413
Fragments arranged by size:
2413 1656 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 2666 5442 2666
Size: 2776 2962
BsaAI yAC'GTr
Cuts at: 3091 3091
Size: 5738
BsaBI GATnn'nnATC
Cuts at: 6 3252 5289 6
Size: 3246 2037 455
Fragments arranged by size:
3246 2037 455
BsaHI Gr'CG_yC
Cuts at: 204 464 1322 1735 3169 3417 4119 5615 204
Size: 260 858 413 1434 248 702 1496 327
Fragments arranged by size:
1496 1434 858 702 413 327 260 248
BsaWI w'CCGG_w
Cuts at: 284 644 1166 2927 3448 4126 4400 4547 4958
Size: 360 522 1761 521 678 274 147 411
Cuts at: 4958 284
Size: 1064
Fragments arranged by size:
1761 1064 678 522 521 411 360 274
147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 363 565 703 934 937 940 943 1261 1264
Size: 202 138 231 3 3 3 318 3
Cuts at: 1264 1273 1494 1834 1837 1840 1844 1876 1930
Size: 9 221 340 3 3 4 32 54
Cuts at: 1930 2119 363
Size: 189 3982
Fragments arranged by size:
3982 340 318 231 221 202 189 138
54 32 9 4 3 3 3 3
3 3
BseYI C'CCAG_C
Cuts at: 409 507 955 1469 1499 1637 1833 2382 2408
Size: 98 448 514 30 138 196 549 26
Cuts at: 2408 3163 3921 4498 4994 409
Size: 755 758 577 496 1153
Fragments arranged by size:
1153 758 755 577 549 514 496 448
196 138 98 30 26
BsiEI CG_ry'CG
Cuts at: 104 135 180 1351 1515 3280 3326 4534 104
Size: 31 45 1171 164 1765 46 1208 1308
Fragments arranged by size:
1765 1308 1208 1171 164 46 45 31
BsiHKAI G_wGCw'C
Cuts at: 368 2752 3530 3720 4512 368
Size: 2384 778 190 792 1594
Fragments arranged by size:
2384 1594 792 778 190
BsmI GAATG_Cn'
Cuts at: 1962 1962
Size: 5738
BsmBI CGTCTCn'nnnn_
Cuts at: 595 1567 595
Size: 972 4766
Bsp1286I G_dGCh'C
Cuts at: 368 427 756 956 1469 1585 2048 2752 3363
Size: 59 329 200 513 116 463 704 611
Cuts at: 3363 3456 3530 3720 3782 4512 368
Size: 93 74 190 62 730 1594
Fragments arranged by size:
1594 730 704 611 513 463 329 200
190 116 93 74 62 59
BspEI T'CCGG_A
Cuts at: 644 4958 644
Size: 4314 1424
BspHI T'CATG_A
Cuts at: 4914 4914
Size: 5738
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1372 1556 1958 2788 3002 3304 3685 1372
Size: 184 402 830 214 302 381 3425
Fragments arranged by size:
3425 830 402 381 302 214 184
BsrBI CCG'CTC
Cuts at: 50 1235 1905 4030 4084 5568 5733 50
Size: 1185 670 2125 54 1484 165 55
Fragments arranged by size:
2125 1484 1185 670 165 55 54
BsrDI GCAATG_nn'
Cuts at: 515 3650 515
Size: 3135 2603
BsrFI r'CCGG_y
Cuts at: 3736 3917 4126 3736
Size: 181 209 5348
Fragments arranged by size:
5348 209 181
BssSI C'ACGA_G
Cuts at: 1749 4009 4367 1749
Size: 2260 358 3120
Fragments arranged by size:
3120 2260 358
BstAPI GCAn_nnn'nTGC
Cuts at: 657 1653 3115 657
Size: 996 1462 3280
Fragments arranged by size:
3280 1462 996
BstBI TT'CG_AA
Cuts at: 4099 4099
Size: 5738
BstEII G'GTnAC_C
Cuts at: 1503 1503
Size: 5738
BstXI CCAn_nnnn'nTGG
Cuts at: 2416 2416
Size: 5738
BstYI r'GATC_y
Cuts at: 1 1535 2763 3230 3253 3588 3834 4846 4932
Size: 1534 1228 467 23 335 246 1012 86
Cuts at: 4932 4944 5290 1
Size: 12 346 449
Fragments arranged by size:
1534 1228 1012 467 449 346 335 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1859 1871 1859
Size: 12 5726
BtgI C'CryG_G
Cuts at: 107 240 1325 1345 107
Size: 133 1085 20 4500
Fragments arranged by size:
4500 1085 133 20
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1371 3688 3869 4032 1371
Size: 2317 181 163 3077
Fragments arranged by size:
3077 2317 181 163
BtsI GCAGTG_nn'
Cuts at: 1524 2981 1524
Size: 1457 4281
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 2004 2039 2004
Size: 35 5703
DraI TTT'AAA
Cuts at: 2737 2737
Size: 5738
DrdI GACnn_nn'nnGTC
Cuts at: 3444 4302 3444
Size: 858 4880
EaeI y'GGCC_r
Cuts at: 243 1236 1348 1676 3323 3497 3888 3915 5260
Size: 993 112 328 1647 174 391 27 1345
Cuts at: 5260 243
Size: 721
Fragments arranged by size:
1647 1345 993 721 391 328 174 112
27
EagI C'GGCC_G
Cuts at: 1348 3323 1348
Size: 1975 3763
EarI CTCTTCn'nnn_
Cuts at: 153 1106 1378 2147 3761 3971 153
Size: 953 272 769 1614 210 1920
Fragments arranged by size:
1920 1614 953 769 272 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2533 3946 4256 4402 5548 2533
Size: 1413 310 146 1146 2723
Fragments arranged by size:
2723 1413 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2750 2750
Size: 5738
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 931 1001 1354 2104 3563 3995 4742 5010 931
Size: 70 353 750 1459 432 747 268 1659
Fragments arranged by size:
1659 1459 750 747 432 353 268 70
EcoNI CCTnn'n_nnAGG
Cuts at: 913 1801 1874 913
Size: 888 73 4777
Fragments arranged by size:
4777 888 73
EcoO109I rG'GnC_Cy
Cuts at: 423 752 753 1599 2044 2275 2484 2566 2700
Size: 329 1 846 445 231 209 82 134
Cuts at: 2700 2877 423
Size: 177 3284
Fragments arranged by size:
3284 846 445 329 231 209 177 134
82 1
EcoRI G'AATT_C
Cuts at: 2742 2742
Size: 5738
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3757 3757
Size: 5738
FspI TGC'GCA
Cuts at: 1381 1657 1954 2290 3519 1381
Size: 276 297 336 1229 3600
Fragments arranged by size:
3600 1229 336 297 276
HaeII r_GCGC'y
Cuts at: 255 303 621 1273 1459 1817 1853 2382 3172
Size: 48 318 652 186 358 36 529 790
Cuts at: 3172 3420 4442 5421 255
Size: 248 1022 979 572
Fragments arranged by size:
1022 979 790 652 572 529 358 318
248 186 48 36
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 758 790 1204 1210 1236 1242 2001 2033 3580
Size: 32 414 6 26 6 759 32 1547
Cuts at: 3580 3610 3612 3642 758
Size: 30 2 30 2854
Fragments arranged by size:
2854 1547 759 414 32 32 30 30
26 6 6 2
HincII GTy'rAC
Cuts at: 1524 2777 1524
Size: 1253 4485
HpaI GTT'AAC
Cuts at: 1524 1524
Size: 5738
KasI G'GCGC_C
Cuts at: 3168 3416 3168
Size: 248 5490
KpnI G_GTAC'C
Cuts at: 1437 2758 1437
Size: 1321 4417
MluI A'CGCG_T
Cuts at: 5171 5171
Size: 5738
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1070 1202 1522 2237 4409 4593 1070
Size: 132 320 715 2172 184 2215
Fragments arranged by size:
2215 2172 715 320 184 132
MscI TGG'CCA
Cuts at: 3499 3499
Size: 5738
MslI CAynn'nnrTG
Cuts at: 683 716 1178 3727 3854 683
Size: 33 462 2549 127 2567
Fragments arranged by size:
2567 2549 462 127 33
MspA1I CmG'CkG
Cuts at: 1327 1347 1353 1844 2849 3163 3523 4536 4781
Size: 20 6 491 1005 314 360 1013 245
Cuts at: 4781 1327
Size: 2284
Fragments arranged by size:
2284 1013 1005 491 360 314 245 20
6
NaeI GCC'GGC
Cuts at: 3919 3919
Size: 5738
NarI GG'CG_CC
Cuts at: 3169 3417 3169
Size: 248 5490
NcoI C'CATG_G
Cuts at: 107 107
Size: 5738
NgoMIV G'CCGG_C
Cuts at: 3917 3917
Size: 5738
NotI GC'GGCC_GC
Cuts at: 1348 1348
Size: 5738
NspI r_CATG'y
Cuts at: 2314 2791 3291 4198 2314
Size: 477 500 907 3854
Fragments arranged by size:
3854 907 500 477
PasI CC'CwG_GG
Cuts at: 449 2481 449
Size: 2032 3706
PciI A'CATG_T
Cuts at: 4194 4194
Size: 5738
PflMI CCAn_nnn'nTGG
Cuts at: 433 1695 2166 433
Size: 1262 471 4005
Fragments arranged by size:
4005 1262 471
PfoI T'CCnGG_A
Cuts at: 818 908 1565 818
Size: 90 657 4991
Fragments arranged by size:
4991 657 90
PmeI GTTT'AAAC
Cuts at: 2737 2737
Size: 5738
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4877 4909 4877
Size: 32 5706
PpuMI rG'GwC_Cy
Cuts at: 1599 2275 2700 1599
Size: 676 425 4637
Fragments arranged by size:
4637 676 425
PspOMI G'GGCC_C
Cuts at: 423 752 2044 423
Size: 329 1292 4117
Fragments arranged by size:
4117 1292 329
PstI C_TGCA'G
Cuts at: 1553 2553 2785 3470 1553
Size: 1000 232 685 3821
Fragments arranged by size:
3821 1000 685 232
PvuI CG_AT'CG
Cuts at: 104 135 104
Size: 31 5707
PvuII CAG'CTG
Cuts at: 3163 3523 3163
Size: 360 5378
RsrII CG'GwC_CG
Cuts at: 3933 3933
Size: 5738
SacI G_AGCT'C
Cuts at: 2752 2752
Size: 5738
SacII CC_GC'GG
Cuts at: 1328 1348 1328
Size: 20 5718
SalI G'TCGA_C
Cuts at: 2775 2775
Size: 5738
SapI GCTCTTCn'nnn_
Cuts at: 3761 3971 3761
Size: 210 5528
SbfI CC_TGCA'GG
Cuts at: 2785 2785
Size: 5738
ScaI AGT'ACT
Cuts at: 5255 5255
Size: 5738
SfcI C'TryA_G
Cuts at: 33 1549 2549 2781 3466 4459 4650 33
Size: 1516 1000 232 685 993 191 1121
Fragments arranged by size:
1516 1121 1000 993 685 232 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 751 751
Size: 5738
SfoI GGC'GCC
Cuts at: 3170 3418 3170
Size: 248 5490
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5738
SmaI CCC'GGG
Cuts at: 748 1405 2760 748
Size: 657 1355 3726
Fragments arranged by size:
3726 1355 657
SmlI C'TyrA_G
Cuts at: 1273 2893 3808 4300 4562 1273
Size: 1620 915 492 262 2449
Fragments arranged by size:
2449 1620 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5738
SphI G_CATG'C
Cuts at: 2314 2791 3291 2314
Size: 477 500 4761
Fragments arranged by size:
4761 500 477
StuI AGG'CCT
Cuts at: 989 989
Size: 5738
StyI C'CwwG_G
Cuts at: 107 990 1292 1316 1967 2677 2872 3226 107
Size: 883 302 24 651 710 195 354 2619
Fragments arranged by size:
2619 883 710 651 354 302 195 24
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 100 523 689 728 1491 1651 2070 3321 3454
Size: 423 166 39 763 160 419 1251 133
Cuts at: 3454 3913 4122 100
Size: 459 209 1716
Fragments arranged by size:
1716 1251 763 459 423 419 209 166
160 133 39
TatI w'GTAC_w
Cuts at: 5253 5253
Size: 5738
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1161 1193 2652 2684 5255 5287 1161
Size: 32 1459 32 2571 32 1612
Fragments arranged by size:
2571 1612 1459 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 3535 3535
Size: 5738
XbaI T'CTAG_A
Cuts at: 2769 2769
Size: 5738
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2587 3233 2587
Size: 646 5092
XmaI C'CCGG_G
Cuts at: 746 1403 2758 746
Size: 657 1355 3726
Fragments arranged by size:
3726 1355 657
XmnI GAAnn'nnTTC
Cuts at: 559 559
Size: 5738
ZraI GAC'GTC
Cuts at: 205 465 205
Size: 260 5478
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflIII AgeI
AhdI AleI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI
BciVI BclI BglI BglII BlpI Bme1580I BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseRI BseYI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HpaI KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PspOMI PstI PvuI
PvuII RsrII SacI SacII SalI SapI SbfI ScaI
SfcI SfiI SfoI SgfI SmaI SmlI SpeI SphI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AflII AlfI AloI AscI BaeI BcgI BfrBI
BmgBI BmtI BsaXI BsgI BsiWI BsrGI BssHII BstZ17I
ClaI DraIII EcoRV FseI FspAI HindIII MfeI NdeI
NheI NruI NsiI PacI PmlI PshAI PsiI PspXI
PsrI SanDI SexAI SgrAI SnaBI SrfI SspI SwaI
XhoI