Restriction map for
pF1KB5797
(Circular) (Six-base) MAPSORT of: pF1KB5797.seq Check: 4528 from: 1 to: 5615
pF1KB5797 5615 bp
With 182 enzymes: SgfI *
April 24, 2009 13:05 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2394 2394
Size: 5615
AatII G_ACGT'C
Cuts at: 635 635
Size: 5615
AccI GT'mk_AC
Cuts at: 602 671 2653 602
Size: 69 1982 3564
Fragments arranged by size:
3564 1982 69
Acc65I G'GTAC_C
Cuts at: 2631 2631
Size: 5615
AclI AA'CG_TT
Cuts at: 2605 5455 2605
Size: 2850 2765
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1330 1504 1761 1825 2280 3440 3872 4619 4887
Size: 174 257 64 455 1160 432 747 268
Cuts at: 4887 1330
Size: 2058
Fragments arranged by size:
2058 1160 747 455 432 268 257 174
64
AfeI AGC'GCT
Cuts at: 2584 2584
Size: 5615
AflIII A'CryG_T
Cuts at: 1244 2967 4071 5048 1244
Size: 1723 1104 977 1811
Fragments arranged by size:
1811 1723 1104 977
AgeI A'CCGG_T
Cuts at: 972 1694 4003 972
Size: 722 2309 2584
Fragments arranged by size:
2584 2309 722
AleI CACnn'nnGTG
Cuts at: 1459 1459
Size: 5615
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 332 2426 332
Size: 2094 3521
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1532 1564 1532
Size: 32 5583
AlwNI CAG_nnn'CTG
Cuts at: 964 996 1602 1872 2229 2920 4487 964
Size: 32 606 270 357 691 1567 2092
Fragments arranged by size:
2092 1567 691 606 357 270 32
ApaI G_GGCC'C
Cuts at: 551 551
Size: 5615
ApaLI G'TGCA_C
Cuts at: 2363 2539 4385 2363
Size: 176 1846 3593
Fragments arranged by size:
3593 1846 176
ApoI r'AATT_y
Cuts at: 455 2619 4968 5224 455
Size: 2164 2349 256 846
Fragments arranged by size:
2349 2164 846 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5615
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5615
AvaI C'yCGr_G
Cuts at: 410 1067 1181 2635 410
Size: 657 114 1454 3390
Fragments arranged by size:
3390 1454 657 114
BamHI G'GATC_C
Cuts at: 1320 2640 1320
Size: 1320 4295
BanI G'GyrC_C
Cuts at: 481 507 986 1719 2631 3045 3293 3328 481
Size: 26 479 733 912 414 248 35 2768
Fragments arranged by size:
2768 912 733 479 414 248 35 26
BanII G_rGCy'C
Cuts at: 551 1042 2629 3659 551
Size: 491 1587 1030 2507
Fragments arranged by size:
2507 1587 1030 491
BbeI G_GCGC'C
Cuts at: 3049 3297 3049
Size: 248 5367
BbsI GAAGACnn'nnnn_
Cuts at: 1247 1592 1247
Size: 345 5270
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 319 353 319
Size: 34 5581
BciVI GTATCCnnnnn_n'
Cuts at: 3513 4274 5615 3513
Size: 761 1341 3513
Fragments arranged by size:
3513 1341 761
BclI T'GATC_A
Cuts at: 3135 3135
Size: 5615
BglII A'GATC_T
Cuts at: 3130 5167 3130
Size: 2037 3578
BlpI GC'TnA_GC
Cuts at: 2104 2310 2727 2104
Size: 206 417 4992
Fragments arranged by size:
4992 417 206
Bme1580I G_kGCm'C
Cuts at: 551 1724 2367 2543 3240 3333 4389 551
Size: 1173 643 176 697 93 1056 1777
Fragments arranged by size:
1777 1173 1056 697 643 176 93
BmgBI CAC'GTC
Cuts at: 1253 1253
Size: 5615
BmrI ACTGGGnnnn_n'
Cuts at: 2827 2908 3243 5387 2827
Size: 81 335 2144 3055
Fragments arranged by size:
3055 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 336 3982 336
Size: 3646 1969
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 306 1203 1339 1365 2427 2614 306
Size: 897 136 26 1062 187 3307
Fragments arranged by size:
3307 1062 897 187 136 26
Bpu10I CC'TnA_GC
Cuts at: 814 1298 1927 2213 814
Size: 484 629 286 4216
Fragments arranged by size:
4216 629 484 286
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 647 722 1105 1387 2452 2791 3670 4162 4460
Size: 75 383 282 1065 339 879 492 298
Cuts at: 4460 647
Size: 1802
Fragments arranged by size:
1802 1065 879 492 383 339 298 282
75
BsaI GGTCTCn'nnnn_
Cuts at: 2248 5319 2248
Size: 3071 2544
BsaAI yAC'GTr
Cuts at: 555 600 2968 555
Size: 45 2368 3202
Fragments arranged by size:
3202 2368 45
BsaBI GATnn'nnATC
Cuts at: 6 3129 5166 6
Size: 3123 2037 455
Fragments arranged by size:
3123 2037 455
BsaHI Gr'CG_yC
Cuts at: 632 1730 3046 3294 3996 5492 632
Size: 1098 1316 248 702 1496 755
Fragments arranged by size:
1496 1316 1098 755 702 248
BsaWI w'CCGG_w
Cuts at: 876 972 1694 2804 3325 4003 4277 4424 4835
Size: 96 722 1110 521 678 274 147 411
Cuts at: 4835 876
Size: 1656
Fragments arranged by size:
1656 1110 722 678 521 411 274 147
96
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1532 1562 1840 1870 1532
Size: 30 278 30 5277
Fragments arranged by size:
5277 278 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1254 1989 2035 1254
Size: 735 46 4834
Fragments arranged by size:
4834 735 46
BseYI C'CCAG_C
Cuts at: 1293 2315 2512 2570 3040 3798 4375 4871 1293
Size: 1022 197 58 470 758 577 496 2037
Fragments arranged by size:
2037 1022 758 577 496 470 197 58
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 541 891 1653 541
Size: 350 762 4503
Fragments arranged by size:
4503 762 350
BsiEI CG_ry'CG
Cuts at: 104 258 3157 3203 4411 104
Size: 154 2899 46 1208 1308
Fragments arranged by size:
2899 1308 1208 154 46
BsiHKAI G_wGCw'C
Cuts at: 247 2214 2367 2543 2629 3407 3597 4389 247
Size: 1967 153 176 86 778 190 792 1473
Fragments arranged by size:
1967 1473 792 778 190 176 153 86
Bsp1286I G_dGCh'C
Cuts at: 247 551 1042 1724 2214 2367 2543 2629 3240
Size: 304 491 682 490 153 176 86 611
Cuts at: 3240 3333 3407 3597 3659 4389 247
Size: 93 74 190 62 730 1473
Fragments arranged by size:
1473 730 682 611 491 490 304 190
176 153 93 86 74 62
BspEI T'CCGG_A
Cuts at: 4835 4835
Size: 5615
BspHI T'CATG_A
Cuts at: 1826 2066 2435 4791 1826
Size: 240 369 2356 2650
Fragments arranged by size:
2650 2356 369 240
BspMI ACCTGCnnnn'nnnn_
Cuts at: 913 2394 2665 2879 3181 3562 913
Size: 1481 271 214 302 381 2966
Fragments arranged by size:
2966 1481 381 302 271 214
BsrBI CCG'CTC
Cuts at: 50 3907 3961 5445 5610 50
Size: 3857 54 1484 165 55
Fragments arranged by size:
3857 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3527 3527
Size: 5615
BsrFI r'CCGG_y
Cuts at: 972 1694 3613 3794 4003 972
Size: 722 1919 181 209 2584
Fragments arranged by size:
2584 1919 722 209 181
BssSI C'ACGA_G
Cuts at: 328 3886 4244 328
Size: 3558 358 1699
Fragments arranged by size:
3558 1699 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1525 2992 1525
Size: 1467 4148
BstBI TT'CG_AA
Cuts at: 3976 3976
Size: 5615
BstEII G'GTnAC_C
Cuts at: 1882 1882
Size: 5615
BstXI CCAn_nnnn'nTGG
Cuts at: 1389 2481 1389
Size: 1092 4523
BstYI r'GATC_y
Cuts at: 1 1320 1598 2640 3107 3130 3465 3711 4723
Size: 1319 278 1042 467 23 335 246 1012
Cuts at: 4723 4809 4821 5167 1
Size: 86 12 346 449
Fragments arranged by size:
1319 1042 1012 467 449 346 335 278
246 86 23 12
BstZ17I GTA'TAC
Cuts at: 603 672 603
Size: 69 5546
BtgI C'CryG_G
Cuts at: 477 591 2144 2519 477
Size: 114 1553 375 3573
Fragments arranged by size:
3573 1553 375 114
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3565 3746 3909 3565
Size: 181 163 5271
Fragments arranged by size:
5271 181 163
BtsI GCAGTG_nn'
Cuts at: 1466 1912 1965 2858 1466
Size: 446 53 893 4223
Fragments arranged by size:
4223 893 446 53
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 2126 2161 2126
Size: 35 5580
DraI TTT'AAA
Cuts at: 2614 2614
Size: 5615
DraIII CAC_nnn'GTG
Cuts at: 1852 2547 1852
Size: 695 4920
DrdI GACnn_nn'nnGTC
Cuts at: 3321 4179 3321
Size: 858 4757
EaeI y'GGCC_r
Cuts at: 366 474 1655 2495 3200 3374 3765 3792 5137
Size: 108 1181 840 705 174 391 27 1345
Cuts at: 5137 366
Size: 844
Fragments arranged by size:
1345 1181 844 840 705 391 174 108
27
EagI C'GGCC_G
Cuts at: 3200 3200
Size: 5615
EarI CTCTTCn'nnn_
Cuts at: 1318 1780 2299 3638 3848 1318
Size: 462 519 1339 210 3085
Fragments arranged by size:
3085 1339 519 462 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 773 3823 4133 4279 5425 773
Size: 3050 310 146 1146 963
Fragments arranged by size:
3050 1146 963 310 146
EcoICRI GAG'CTC
Cuts at: 2627 2627
Size: 5615
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 306 1203 1330 1339 1365 1504 1761 1825 2280
Size: 897 127 9 26 139 257 64 455
Cuts at: 2280 2427 2614 3440 3872 4619 4887 306
Size: 147 187 826 432 747 268 1034
Fragments arranged by size:
1034 897 826 747 455 432 268 257
187 147 139 127 64 26 9
EcoNI CCTnn'n_nnAGG
Cuts at: 764 1930 764
Size: 1166 4449
EcoO109I rG'GnC_Cy
Cuts at: 134 548 700 927 1362 1770 1941 2754 134
Size: 414 152 227 435 408 171 813 2995
Fragments arranged by size:
2995 813 435 414 408 227 171 152
EcoRI G'AATT_C
Cuts at: 2619 2619
Size: 5615
EcoRV GAT'ATC
Cuts at: 642 642
Size: 5615
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 829 3634 829
Size: 2805 2810
FspI TGC'GCA
Cuts at: 3396 3396
Size: 5615
HaeII r_GCGC'y
Cuts at: 2586 3049 3297 4319 5298 2586
Size: 463 248 1022 979 2903
Fragments arranged by size:
2903 1022 979 463 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 404 436 598 630 720 752 1590 1622 3457
Size: 32 162 32 90 32 838 32 1835
Cuts at: 3457 3487 3489 3519 404
Size: 30 2 30 2500
Fragments arranged by size:
2500 1835 838 162 90 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 2654 2654
Size: 5615
HindIII A'AGCT_T
Cuts at: 289 289
Size: 5615
KasI G'GCGC_C
Cuts at: 3045 3293 3045
Size: 248 5367
KpnI G_GTAC'C
Cuts at: 2635 2635
Size: 5615
MluI A'CGCG_T
Cuts at: 5048 5048
Size: 5615
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 925 2102 4286 4470 925
Size: 1177 2184 184 2070
Fragments arranged by size:
2184 2070 1177 184
MscI TGG'CCA
Cuts at: 368 476 1657 3376 368
Size: 108 1181 1719 2607
Fragments arranged by size:
2607 1719 1181 108
MslI CAynn'nnrTG
Cuts at: 1442 1459 1880 2479 3604 3731 1442
Size: 17 421 599 1125 127 3326
Fragments arranged by size:
3326 1125 599 421 127 17
MspA1I CmG'CkG
Cuts at: 1350 1637 1869 2088 2103 2118 2574 2726 3040
Size: 287 232 219 15 15 456 152 314
Cuts at: 3040 3400 4413 4658 1350
Size: 360 1013 245 2307
Fragments arranged by size:
2307 1013 456 360 314 287 245 232
219 152 15 15
NaeI GCC'GGC
Cuts at: 3796 3796
Size: 5615
NarI GG'CG_CC
Cuts at: 3046 3294 3046
Size: 248 5367
NcoI C'CATG_G
Cuts at: 591 591
Size: 5615
NdeI CA'TA_TG
Cuts at: 1056 1056
Size: 5615
NgoMIV G'CCGG_C
Cuts at: 3794 3794
Size: 5615
NspI r_CATG'y
Cuts at: 1248 2668 3168 4075 1248
Size: 1420 500 907 2788
Fragments arranged by size:
2788 1420 907 500
PasI CC'CwG_GG
Cuts at: 1860 1860
Size: 5615
PciI A'CATG_T
Cuts at: 1244 4071 1244
Size: 2827 2788
PflMI CCAn_nnn'nTGG
Cuts at: 1852 2121 1852
Size: 269 5346
PfoI T'CCnGG_A
Cuts at: 957 1316 1535 957
Size: 359 219 5037
Fragments arranged by size:
5037 359 219
PmeI GTTT'AAAC
Cuts at: 2614 2614
Size: 5615
PmlI CAC'GTG
Cuts at: 555 555
Size: 5615
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1532 1564 4754 4786 1532
Size: 32 3190 32 2361
Fragments arranged by size:
3190 2361 32 32
PpuMI rG'GwC_Cy
Cuts at: 927 927
Size: 5615
PshAI GACnn'nnGTC
Cuts at: 2476 2476
Size: 5615
PspOMI G'GGCC_C
Cuts at: 547 547
Size: 5615
PstI C_TGCA'G
Cuts at: 969 2531 2662 3347 969
Size: 1562 131 685 3237
Fragments arranged by size:
3237 1562 685 131
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5615
PvuII CAG'CTG
Cuts at: 1637 1869 2088 2103 2118 2574 3040 3400 1637
Size: 232 219 15 15 456 466 360 3852
Fragments arranged by size:
3852 466 456 360 232 219 15 15
RsrII CG'GwC_CG
Cuts at: 3810 3810
Size: 5615
SacI G_AGCT'C
Cuts at: 2629 2629
Size: 5615
SalI G'TCGA_C
Cuts at: 2652 2652
Size: 5615
SapI GCTCTTCn'nnn_
Cuts at: 1318 3638 3848 1318
Size: 2320 210 3085
Fragments arranged by size:
3085 2320 210
SbfI CC_TGCA'GG
Cuts at: 2531 2662 2531
Size: 131 5484
ScaI AGT'ACT
Cuts at: 5132 5132
Size: 5615
SfcI C'TryA_G
Cuts at: 33 965 1950 2008 2527 2658 3343 4336 4527
Size: 932 985 58 519 131 685 993 191
Cuts at: 4527 33
Size: 1121
Fragments arranged by size:
1121 993 985 932 685 519 191 131
58
SfoI GGC'GCC
Cuts at: 3047 3295 3047
Size: 248 5367
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5615
SmaI CCC'GGG
Cuts at: 2637 2637
Size: 5615
SmlI C'TyrA_G
Cuts at: 662 737 1084 1366 2467 2770 3685 4177 4439
Size: 75 347 282 1101 303 915 492 262
Cuts at: 4439 662
Size: 1838
Fragments arranged by size:
1838 1101 915 492 347 303 282 262
75
SnaBI TAC'GTA
Cuts at: 600 600
Size: 5615
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5615
SphI G_CATG'C
Cuts at: 2668 3168 2668
Size: 500 5115
StuI AGG'CCT
Cuts at: 1925 1925
Size: 5615
StyI C'CwwG_G
Cuts at: 591 1268 2749 3103 591
Size: 677 1481 354 3103
Fragments arranged by size:
3103 1481 677 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 499 1025 1363 3198 3331 3790 3999 499
Size: 526 338 1835 133 459 209 2115
Fragments arranged by size:
2115 1835 526 459 338 209 133
TatI w'GTAC_w
Cuts at: 1222 5130 1222
Size: 3908 1707
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 220 252 1019 1051 1840 1872 5132 5164 220
Size: 32 767 32 789 32 3260 32 671
Fragments arranged by size:
3260 789 767 671 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 148 3412 148
Size: 3264 2351
XbaI T'CTAG_A
Cuts at: 2560 2646 2560
Size: 86 5529
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 469 1129 3110 469
Size: 660 1981 2974
Fragments arranged by size:
2974 1981 660
XmaI C'CCGG_G
Cuts at: 2635 2635
Size: 5615
ZraI GAC'GTC
Cuts at: 633 633
Size: 5615
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AgeI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglII BlpI Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI
HaeII Hin4I HincII HindIII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PspOMI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SnaBI SpeI SphI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI ZraI
Enzymes that do not cut:
AflII AhdI AscI AvrII BaeI BbvCI BfrBI BglI
BmtI BsiWI BsmI BsmBI BsrGI BssHII Bsu36I ClaI
FseI FspAI HpaI MfeI NheI NotI NruI NsiI
PacI PsiI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SrfI SspI SwaI XhoI XmnI