Restriction map for
pF1KB5786
(Circular) (Six-base) MAPSORT of: pF1KB5786.seq Check: 5233 from: 1 to: 5300
pF1KB5786 5300 bp
With 182 enzymes: SgfI *
October 7, 2009 14:17 ..
AccI GT'mk_AC
Cuts at: 396 937 2338 396
Size: 541 1401 3358
Fragments arranged by size:
3358 1401 541
Acc65I G'GTAC_C
Cuts at: 2316 2316
Size: 5300
AclI AA'CG_TT
Cuts at: 963 5140 963
Size: 4177 1123
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 393 1196 3125 3557 4304 4572 393
Size: 803 1929 432 747 268 1121
Fragments arranged by size:
1929 1121 803 747 432 268
AfeI AGC'GCT
Cuts at: 2119 2119
Size: 5300
AflIII A'CryG_T
Cuts at: 161 737 932 2652 3756 4733 161
Size: 576 195 1720 1104 977 728
Fragments arranged by size:
1720 1104 977 728 576 195
AgeI A'CCGG_T
Cuts at: 3688 3688
Size: 5300
AlwNI CAG_nnn'CTG
Cuts at: 769 1603 2605 4172 769
Size: 834 1002 1567 1897
Fragments arranged by size:
1897 1567 1002 834
ApaLI G'TGCA_C
Cuts at: 4070 4070
Size: 5300
ApoI r'AATT_y
Cuts at: 303 580 712 920 1139 1454 2304 4653 4909
Size: 277 132 208 219 315 850 2349 256
Cuts at: 4909 303
Size: 694
Fragments arranged by size:
2349 850 694 315 277 256 219 208
132
AseI AT'TA_AT
Cuts at: 20 20
Size: 5300
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5300
AvaI C'yCGr_G
Cuts at: 1814 2050 2320 1814
Size: 236 270 4794
Fragments arranged by size:
4794 270 236
BamHI G'GATC_C
Cuts at: 2046 2325 2046
Size: 279 5021
BanI G'GyrC_C
Cuts at: 2316 2730 2978 3013 2316
Size: 414 248 35 4603
Fragments arranged by size:
4603 414 248 35
BanII G_rGCy'C
Cuts at: 219 776 2314 3344 219
Size: 557 1538 1030 2175
Fragments arranged by size:
2175 1538 1030 557
BbeI G_GCGC'C
Cuts at: 2734 2982 2734
Size: 248 5052
BciVI GTATCCnnnnn_n'
Cuts at: 1412 3198 3959 5300 1412
Size: 1786 761 1341 1412
Fragments arranged by size:
1786 1412 1341 761
BclI T'GATC_A
Cuts at: 1704 2820 1704
Size: 1116 4184
BglII A'GATC_T
Cuts at: 574 2815 4852 574
Size: 2241 2037 1022
Fragments arranged by size:
2241 2037 1022
BlpI GC'TnA_GC
Cuts at: 1967 2412 1967
Size: 445 4855
Bme1580I G_kGCm'C
Cuts at: 2153 2925 3018 4074 2153
Size: 772 93 1056 3379
Fragments arranged by size:
3379 1056 772 93
BmgBI CAC'GTC
Cuts at: 164 164
Size: 5300
BmrI ACTGGGnnnn_n'
Cuts at: 2512 2593 2928 5072 2512
Size: 81 335 2144 2740
Fragments arranged by size:
2740 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3667 3667
Size: 5300
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1490 1700 2040 1490
Size: 210 340 4750
Fragments arranged by size:
4750 340 210
Bpu10I CC'TnA_GC
Cuts at: 210 770 1262 210
Size: 560 492 4248
Fragments arranged by size:
4248 560 492
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 628 2476 3355 3847 4145 628
Size: 1848 879 492 298 1783
Fragments arranged by size:
1848 1783 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 632 5004 632
Size: 4372 928
BsaAI yAC'GTr
Cuts at: 2653 2653
Size: 5300
BsaBI GATnn'nnATC
Cuts at: 6 2814 4851 6
Size: 2808 2037 455
Fragments arranged by size:
2808 2037 455
BsaHI Gr'CG_yC
Cuts at: 180 2087 2731 2979 3681 5177 180
Size: 1907 644 248 702 1496 303
Fragments arranged by size:
1907 1496 702 644 303 248
BsaWI w'CCGG_w
Cuts at: 2219 2489 3010 3688 3962 4109 4520 2219
Size: 270 521 678 274 147 411 2999
Fragments arranged by size:
2999 678 521 411 274 270 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 509 539 509
Size: 30 5270
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1298 1298
Size: 5300
BseYI C'CCAG_C
Cuts at: 1858 2725 3483 4060 4556 1858
Size: 867 758 577 496 2602
Fragments arranged by size:
2602 867 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 266 1342 1766 2188 266
Size: 1076 424 422 3378
Fragments arranged by size:
3378 1076 424 422
BsiEI CG_ry'CG
Cuts at: 104 2842 2888 4096 104
Size: 2738 46 1208 1308
Fragments arranged by size:
2738 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 219 776 2314 3092 3282 4074 219
Size: 557 1538 778 190 792 1445
Fragments arranged by size:
1538 1445 792 778 557 190
BsmI GAATG_Cn'
Cuts at: 1533 1533
Size: 5300
Bsp1286I G_dGCh'C
Cuts at: 219 776 2153 2314 2925 3018 3092 3282 3344
Size: 557 1377 161 611 93 74 190 62
Cuts at: 3344 4074 219
Size: 730 1445
Fragments arranged by size:
1445 1377 730 611 557 190 161 93
74 62
BspEI T'CCGG_A
Cuts at: 4520 4520
Size: 5300
BspHI T'CATG_A
Cuts at: 1010 4476 1010
Size: 3466 1834
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2350 2564 2866 3247 2350
Size: 214 302 381 4403
Fragments arranged by size:
4403 381 302 214
BsrBI CCG'CTC
Cuts at: 50 209 3592 3646 5130 5295 50
Size: 159 3383 54 1484 165 55
Fragments arranged by size:
3383 1484 165 159 55 54
BsrDI GCAATG_nn'
Cuts at: 124 714 1962 3212 124
Size: 590 1248 1250 2212
Fragments arranged by size:
2212 1250 1248 590
BsrFI r'CCGG_y
Cuts at: 3298 3479 3688 3298
Size: 181 209 4910
Fragments arranged by size:
4910 209 181
BssSI C'ACGA_G
Cuts at: 2164 3571 3929 2164
Size: 1407 358 3535
Fragments arranged by size:
3535 1407 358
BstAPI GCAn_nnn'nTGC
Cuts at: 2677 2677
Size: 5300
BstBI TT'CG_AA
Cuts at: 464 3661 464
Size: 3197 2103
BstXI CCAn_nnnn'nTGG
Cuts at: 2146 2146
Size: 5300
BstYI r'GATC_y
Cuts at: 1 574 2046 2325 2792 2815 3150 3396 4408
Size: 573 1472 279 467 23 335 246 1012
Cuts at: 4408 4494 4506 4852 1
Size: 86 12 346 449
Fragments arranged by size:
1472 1012 573 467 449 346 335 279
246 86 23 12
BtgI C'CryG_G
Cuts at: 1803 2139 1803
Size: 336 4964
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3250 3431 3594 3250
Size: 181 163 4956
Fragments arranged by size:
4956 181 163
BtsI GCAGTG_nn'
Cuts at: 1284 1424 1485 1644 1781 2176 2543 1284
Size: 140 61 159 137 395 367 4041
Fragments arranged by size:
4041 395 367 159 140 137 61
DraI TTT'AAA
Cuts at: 437 2299 437
Size: 1862 3438
DraIII CAC_nnn'GTG
Cuts at: 1320 1668 1320
Size: 348 4952
DrdI GACnn_nn'nnGTC
Cuts at: 3006 3864 3006
Size: 858 4442
EaeI y'GGCC_r
Cuts at: 1299 1806 2885 3059 3450 3477 4822 1299
Size: 507 1079 174 391 27 1345 1777
Fragments arranged by size:
1777 1345 1079 507 391 174 27
EagI C'GGCC_G
Cuts at: 2885 2885
Size: 5300
EarI CTCTTCn'nnn_
Cuts at: 412 781 2134 3323 3533 412
Size: 369 1353 1189 210 2179
Fragments arranged by size:
2179 1353 1189 369 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1564 3508 3818 3964 5110 1564
Size: 1944 310 146 1146 1754
Fragments arranged by size:
1944 1754 1146 310 146
EcoICRI GAG'CTC
Cuts at: 217 774 2312 217
Size: 557 1538 3205
Fragments arranged by size:
3205 1538 557
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 393 1196 1490 1700 2040 3125 3557 4304 4572
Size: 803 294 210 340 1085 432 747 268
Cuts at: 4572 393
Size: 1121
Fragments arranged by size:
1121 1085 803 747 432 340 294 268
210
EcoO109I rG'GnC_Cy
Cuts at: 119 2439 119
Size: 2320 2980
EcoRI G'AATT_C
Cuts at: 920 2304 920
Size: 1384 3916
EcoRV GAT'ATC
Cuts at: 2234 2234
Size: 5300
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1168 1352 3319 1168
Size: 184 1967 3149
Fragments arranged by size:
3149 1967 184
FspI TGC'GCA
Cuts at: 3081 3081
Size: 5300
HaeII r_GCGC'y
Cuts at: 2121 2734 2982 4004 4983 2121
Size: 613 248 1022 979 2438
Fragments arranged by size:
2438 1022 979 613 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 423 455 618 650 1266 1298 1648 1680 3142
Size: 32 163 32 616 32 350 32 1462
Cuts at: 3142 3172 3174 3204 423
Size: 30 2 30 2519
Fragments arranged by size:
2519 1462 616 350 163 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 2339 2339
Size: 5300
KasI G'GCGC_C
Cuts at: 2730 2978 2730
Size: 248 5052
KpnI G_GTAC'C
Cuts at: 2320 2320
Size: 5300
MluI A'CGCG_T
Cuts at: 4733 4733
Size: 5300
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 412 1467 2063 3971 4155 412
Size: 1055 596 1908 184 1557
Fragments arranged by size:
1908 1557 1055 596 184
MscI TGG'CCA
Cuts at: 1301 3061 1301
Size: 1760 3540
MslI CAynn'nnrTG
Cuts at: 173 2144 3289 3416 173
Size: 1971 1145 127 2057
Fragments arranged by size:
2057 1971 1145 127
MspA1I CmG'CkG
Cuts at: 1654 2411 2725 3085 4098 4343 1654
Size: 757 314 360 1013 245 2611
Fragments arranged by size:
2611 1013 757 360 314 245
NaeI GCC'GGC
Cuts at: 3481 3481
Size: 5300
NarI GG'CG_CC
Cuts at: 2731 2979 2731
Size: 248 5052
NgoMIV G'CCGG_C
Cuts at: 3479 3479
Size: 5300
NspI r_CATG'y
Cuts at: 936 2353 2853 3760 936
Size: 1417 500 907 2476
Fragments arranged by size:
2476 1417 907 500
PciI A'CATG_T
Cuts at: 932 3756 932
Size: 2824 2476
PfoI T'CCnGG_A
Cuts at: 1462 1462
Size: 5300
PmeI GTTT'AAAC
Cuts at: 2299 2299
Size: 5300
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4439 4471 4439
Size: 32 5268
PpuMI rG'GwC_Cy
Cuts at: 119 119
Size: 5300
PstI C_TGCA'G
Cuts at: 603 1431 2347 3032 603
Size: 828 916 685 2871
Fragments arranged by size:
2871 916 828 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5300
PvuII CAG'CTG
Cuts at: 1654 2725 3085 1654
Size: 1071 360 3869
Fragments arranged by size:
3869 1071 360
RsrII CG'GwC_CG
Cuts at: 3495 3495
Size: 5300
SacI G_AGCT'C
Cuts at: 219 776 2314 219
Size: 557 1538 3205
Fragments arranged by size:
3205 1538 557
SalI G'TCGA_C
Cuts at: 2337 2337
Size: 5300
SapI GCTCTTCn'nnn_
Cuts at: 412 781 2134 3323 3533 412
Size: 369 1353 1189 210 2179
Fragments arranged by size:
2179 1353 1189 369 210
SbfI CC_TGCA'GG
Cuts at: 2347 2347
Size: 5300
ScaI AGT'ACT
Cuts at: 1296 4817 1296
Size: 3521 1779
SfcI C'TryA_G
Cuts at: 33 233 273 599 938 1359 1427 1649 2343
Size: 200 40 326 339 421 68 222 694
Cuts at: 2343 3028 4021 4212 33
Size: 685 993 191 1121
Fragments arranged by size:
1121 993 694 685 421 339 326 222
200 191 68 40
SfoI GGC'GCC
Cuts at: 2732 2980 2732
Size: 248 5052
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5300
SmaI CCC'GGG
Cuts at: 1816 2052 2322 1816
Size: 236 270 4794
Fragments arranged by size:
4794 270 236
SmlI C'TyrA_G
Cuts at: 643 2455 3370 3862 4124 643
Size: 1812 915 492 262 1819
Fragments arranged by size:
1819 1812 915 492 262
SpeI A'CTAG_T
Cuts at: 67 843 2035 67
Size: 776 1192 3332
Fragments arranged by size:
3332 1192 776
SphI G_CATG'C
Cuts at: 2353 2853 2353
Size: 500 4800
SspI AAT'ATT
Cuts at: 2262 2262
Size: 5300
StyI C'CwwG_G
Cuts at: 2434 2788 2434
Size: 354 4946
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2883 3016 3475 3684 2883
Size: 133 459 209 4499
Fragments arranged by size:
4499 459 209 133
TatI w'GTAC_w
Cuts at: 1294 4815 1294
Size: 3521 1779
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1458 1490 4817 4849 1458
Size: 32 3327 32 1909
Fragments arranged by size:
3327 1909 32 32
Tth111I GACn'n_nGTC
Cuts at: 3097 3097
Size: 5300
XbaI T'CTAG_A
Cuts at: 999 2331 999
Size: 1332 3968
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 166 2040 2795 166
Size: 1874 755 2671
Fragments arranged by size:
2671 1874 755
XmaI C'CCGG_G
Cuts at: 1814 2050 2320 1814
Size: 236 270 4794
Fragments arranged by size:
4794 270 236
XmnI GAAnn'nnTTC
Cuts at: 460 953 1139 1209 1332 460
Size: 493 186 70 123 4428
Fragments arranged by size:
4428 493 186 123 70
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BciVI BclI BglII BlpI Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NgoMIV NspI PciI PfoI PmeI PpiI PpuMI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
SspI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI
Enzymes that do not cut:
AarI AatII AflII AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbsI BbvCI BcgI BfrBI BglI
BmtI BsiWI BsmBI BsrGI BssHII BstEII BstZ17I Bsu36I
ClaI CspCI EcoNI FseI FspAI HindIII HpaI MfeI
NcoI NdeI NheI NotI NruI NsiI PacI PasI
PflMI PmlI PshAI PsiI PspOMI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI StuI SwaI
XhoI ZraI