Restriction map for
pF1KB5782
(Circular) (Six-base) MAPSORT of: pF1KB5782.seq Check: 5779 from: 1 to: 5420
pF1KB5782 5420 bp
With 182 enzymes: SgfI *
October 7, 2009 14:17 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1508 1508
Size: 5420
AatII G_ACGT'C
Cuts at: 1679 1679
Size: 5420
AccI GT'mk_AC
Cuts at: 2458 2458
Size: 5420
Acc65I G'GTAC_C
Cuts at: 2436 2436
Size: 5420
AclI AA'CG_TT
Cuts at: 5260 5260
Size: 5420
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 702 751 2023 3245 3677 4424 4692 702
Size: 49 1272 1222 432 747 268 1430
Fragments arranged by size:
1430 1272 1222 747 432 268 49
AfeI AGC'GCT
Cuts at: 1735 1735
Size: 5420
AflIII A'CryG_T
Cuts at: 2772 3876 4853 2772
Size: 1104 977 3339
Fragments arranged by size:
3339 1104 977
AgeI A'CCGG_T
Cuts at: 3808 3808
Size: 5420
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2278 2278
Size: 5420
AlwNI CAG_nnn'CTG
Cuts at: 203 252 1481 1584 1659 2090 2725 4292 203
Size: 49 1229 103 75 431 635 1567 1331
Fragments arranged by size:
1567 1331 1229 635 431 103 75 49
ApaI G_GGCC'C
Cuts at: 230 576 2020 230
Size: 346 1444 3630
Fragments arranged by size:
3630 1444 346
ApaLI G'TGCA_C
Cuts at: 180 4190 180
Size: 4010 1410
ApoI r'AATT_y
Cuts at: 1000 2424 4773 5029 1000
Size: 1424 2349 256 1391
Fragments arranged by size:
2349 1424 1391 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5420
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5420
AvaI C'yCGr_G
Cuts at: 157 805 1761 1803 2440 157
Size: 648 956 42 637 3137
Fragments arranged by size:
3137 956 648 637 42
BamHI G'GATC_C
Cuts at: 273 2445 273
Size: 2172 3248
BanI G'GyrC_C
Cuts at: 242 934 1451 1892 2227 2436 2850 3098 3133
Size: 692 517 441 335 209 414 248 35
Cuts at: 3133 242
Size: 2529
Fragments arranged by size:
2529 692 517 441 414 335 248 209
35
BanII G_rGCy'C
Cuts at: 230 576 1775 1819 2020 2434 3464 230
Size: 346 1199 44 201 414 1030 2186
Fragments arranged by size:
2186 1199 1030 414 346 201 44
BbeI G_GCGC'C
Cuts at: 938 2854 3102 938
Size: 1916 248 3256
Fragments arranged by size:
3256 1916 248
BbsI GAAGACnn'nnnn_
Cuts at: 380 1096 1200 380
Size: 716 104 4600
Fragments arranged by size:
4600 716 104
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 591 625 659 693 984 1018 90
Size: 34 467 34 34 34 291 34 4492
Fragments arranged by size:
4492 467 291 34 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3318 4079 5420 3318
Size: 761 1341 3318
Fragments arranged by size:
3318 1341 761
BclI T'GATC_A
Cuts at: 2940 2940
Size: 5420
BfrBI ATG'CAT
Cuts at: 215 215
Size: 5420
BglI GCCn_nnn'nGGC
Cuts at: 605 1805 1922 605
Size: 1200 117 4103
Fragments arranged by size:
4103 1200 117
BglII A'GATC_T
Cuts at: 2935 4972 2935
Size: 2037 3383
BlpI GC'TnA_GC
Cuts at: 1818 2532 1818
Size: 714 4706
Bme1580I G_kGCm'C
Cuts at: 184 230 576 1954 2020 2230 3045 3138 4194
Size: 46 346 1378 66 210 815 93 1056
Cuts at: 4194 184
Size: 1410
Fragments arranged by size:
1410 1378 1056 815 346 210 93 66
46
BmrI ACTGGGnnnn_n'
Cuts at: 1431 2337 2632 2713 3048 5192 1431
Size: 906 295 81 335 2144 1659
Fragments arranged by size:
2144 1659 906 335 295 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2121 3787 2121
Size: 1666 3754
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 991 1137 1185 2155 991
Size: 146 48 970 4256
Fragments arranged by size:
4256 970 146 48
Bpu10I CC'TnA_GC
Cuts at: 1626 2293 1626
Size: 667 4753
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2320 2596 3475 3967 4265 2320
Size: 276 879 492 298 3475
Fragments arranged by size:
3475 879 492 298 276
BsaI GGTCTCn'nnnn_
Cuts at: 1463 5124 1463
Size: 3661 1759
BsaAI yAC'GTr
Cuts at: 726 2773 726
Size: 2047 3373
BsaBI GATnn'nnATC
Cuts at: 6 2934 4971 6
Size: 2928 2037 455
Fragments arranged by size:
2928 2037 455
BsaHI Gr'CG_yC
Cuts at: 821 935 1676 2851 3099 3801 5297 821
Size: 114 741 1175 248 702 1496 944
Fragments arranged by size:
1496 1175 944 741 702 248 114
BsaWI w'CCGG_w
Cuts at: 787 2609 3130 3808 4082 4229 4640 787
Size: 1822 521 678 274 147 411 1567
Fragments arranged by size:
1822 1567 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 682 712 1366 1396 1597 1627 682
Size: 30 654 30 201 30 4475
Fragments arranged by size:
4475 654 201 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1005 1188 1005
Size: 183 5237
BseYI C'CCAG_C
Cuts at: 111 930 1398 1632 2117 2845 3603 4180 4676
Size: 819 468 234 485 728 758 577 496
Cuts at: 4676 111
Size: 855
Fragments arranged by size:
855 819 758 728 577 496 485 468
234
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 219 1336 1444 219
Size: 1117 108 4195
Fragments arranged by size:
4195 1117 108
BsiEI CG_ry'CG
Cuts at: 104 600 960 2962 3008 4216 104
Size: 496 360 2002 46 1208 1308
Fragments arranged by size:
2002 1308 1208 496 360 46
BsiHKAI G_wGCw'C
Cuts at: 184 2434 3212 3402 4194 184
Size: 2250 778 190 792 1410
Fragments arranged by size:
2250 1410 792 778 190
BsmI GAATG_Cn'
Cuts at: 217 1185 2255 217
Size: 968 1070 3382
Fragments arranged by size:
3382 1070 968
Bsp1286I G_dGCh'C
Cuts at: 184 230 576 1775 1819 1954 2020 2230 2434
Size: 46 346 1199 44 135 66 210 204
Cuts at: 2434 3045 3138 3212 3402 3464 4194 184
Size: 611 93 74 190 62 730 1410
Fragments arranged by size:
1410 1199 730 611 346 210 204 190
135 93 74 66 62 46 44
BspEI T'CCGG_A
Cuts at: 787 4640 787
Size: 3853 1567
BspHI T'CATG_A
Cuts at: 314 2390 4596 314
Size: 2076 2206 1138
Fragments arranged by size:
2206 2076 1138
BspMI ACCTGCnnnn'nnnn_
Cuts at: 434 572 822 1416 1508 2098 2470 2684 2986
Size: 138 250 594 92 590 372 214 302
Cuts at: 2986 3367 434
Size: 381 2487
Fragments arranged by size:
2487 594 590 381 372 302 250 214
138 92
BsrBI CCG'CTC
Cuts at: 50 3712 3766 5250 5415 50
Size: 3662 54 1484 165 55
Fragments arranged by size:
3662 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1164 1275 3332 1164
Size: 111 2057 3252
Fragments arranged by size:
3252 2057 111
BsrFI r'CCGG_y
Cuts at: 1448 1889 3418 3599 3808 1448
Size: 441 1529 181 209 3060
Fragments arranged by size:
3060 1529 441 209 181
BsrGI T'GTAC_A
Cuts at: 983 983
Size: 5420
BssSI C'ACGA_G
Cuts at: 3691 4049 3691
Size: 358 5062
BstAPI GCAn_nnn'nTGC
Cuts at: 242 1481 1907 2090 2797 242
Size: 1239 426 183 707 2865
Fragments arranged by size:
2865 1239 707 426 183
BstBI TT'CG_AA
Cuts at: 2009 3781 2009
Size: 1772 3648
BstEII G'GTnAC_C
Cuts at: 1071 1071
Size: 5420
BstXI CCAn_nnnn'nTGG
Cuts at: 1083 1161 1083
Size: 78 5342
BstYI r'GATC_y
Cuts at: 1 273 2445 2912 2935 3270 3516 4528 4614
Size: 272 2172 467 23 335 246 1012 86
Cuts at: 4614 4626 4972 1
Size: 12 346 449
Fragments arranged by size:
2172 1012 467 449 346 335 272 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 535 535
Size: 5420
BtgI C'CryG_G
Cuts at: 1130 1485 1861 2231 1130
Size: 355 376 370 4319
Fragments arranged by size:
4319 376 370 355
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2193 3370 3551 3714 2193
Size: 1177 181 163 3899
Fragments arranged by size:
3899 1177 181 163
BtsI GCAGTG_nn'
Cuts at: 127 1660 2663 127
Size: 1533 1003 2884
Fragments arranged by size:
2884 1533 1003
DraI TTT'AAA
Cuts at: 2419 2419
Size: 5420
DraIII CAC_nnn'GTG
Cuts at: 242 1364 1972 242
Size: 1122 608 3690
Fragments arranged by size:
3690 1122 608
DrdI GACnn_nn'nnGTC
Cuts at: 3126 3984 3126
Size: 858 4562
EaeI y'GGCC_r
Cuts at: 301 597 957 1355 1743 1887 3005 3179 3570
Size: 296 360 398 388 144 1118 174 391
Cuts at: 3570 3597 4942 301
Size: 27 1345 779
Fragments arranged by size:
1345 1118 779 398 391 388 360 296
174 144 27
EagI C'GGCC_G
Cuts at: 597 957 3005 597
Size: 360 2048 3012
Fragments arranged by size:
3012 2048 360
EarI CTCTTCn'nnn_
Cuts at: 3443 3653 3443
Size: 210 5210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3628 3938 4084 5230 3628
Size: 310 146 1146 3818
Fragments arranged by size:
3818 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2432 2432
Size: 5420
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 702 751 991 1137 1185 2023 2155 3245 3677
Size: 49 240 146 48 838 132 1090 432
Cuts at: 3677 4424 4692 702
Size: 747 268 1430
Fragments arranged by size:
1430 1090 838 747 432 268 240 146
132 49 48
EcoNI CCTnn'n_nnAGG
Cuts at: 172 172
Size: 5420
EcoO109I rG'GnC_Cy
Cuts at: 226 573 614 1428 2016 2017 2087 2200 2559
Size: 347 41 814 588 1 70 113 359
Cuts at: 2559 226
Size: 3087
Fragments arranged by size:
3087 814 588 359 347 113 70 41
1
EcoRI G'AATT_C
Cuts at: 1000 2424 1000
Size: 1424 3996
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 273 436 1515 2037 3439 273
Size: 163 1079 522 1402 2254
Fragments arranged by size:
2254 1402 1079 522 163
FspI TGC'GCA
Cuts at: 3201 3201
Size: 5420
HaeII r_GCGC'y
Cuts at: 938 1399 1737 2319 2378 2854 3102 4124 5103
Size: 461 338 582 59 476 248 1022 979
Cuts at: 5103 938
Size: 1255
Fragments arranged by size:
1255 1022 979 582 476 461 338 248
59
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 265 297 997 1029 1597 1629 3262 3292 3294
Size: 32 700 32 568 32 1633 30 2
Cuts at: 3294 3324 265
Size: 30 2361
Fragments arranged by size:
2361 1633 700 568 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 2459 2459
Size: 5420
KasI G'GCGC_C
Cuts at: 934 2850 3098 934
Size: 1916 248 3256
Fragments arranged by size:
3256 1916 248
KpnI G_GTAC'C
Cuts at: 2440 2440
Size: 5420
MluI A'CGCG_T
Cuts at: 4853 4853
Size: 5420
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 773 4091 4275 773
Size: 3318 184 1918
Fragments arranged by size:
3318 1918 184
MscI TGG'CCA
Cuts at: 3181 3181
Size: 5420
MslI CAynn'nnrTG
Cuts at: 2170 3409 3536 2170
Size: 1239 127 4054
Fragments arranged by size:
4054 1239 127
MspA1I CmG'CkG
Cuts at: 203 309 399 780 962 1026 1796 1863 1898
Size: 106 90 381 182 64 770 67 35
Cuts at: 1898 1932 2061 2531 2845 3205 4218 4463 203
Size: 34 129 470 314 360 1013 245 1160
Fragments arranged by size:
1160 1013 770 470 381 360 314 245
182 129 106 90 67 64 35 34
NaeI GCC'GGC
Cuts at: 3601 3601
Size: 5420
NarI GG'CG_CC
Cuts at: 935 2851 3099 935
Size: 1916 248 3256
Fragments arranged by size:
3256 1916 248
NcoI C'CATG_G
Cuts at: 1485 1485
Size: 5420
NgoMIV G'CCGG_C
Cuts at: 3599 3599
Size: 5420
NsiI A_TGCA'T
Cuts at: 217 217
Size: 5420
NspI r_CATG'y
Cuts at: 2473 2973 3880 2473
Size: 500 907 4013
Fragments arranged by size:
4013 907 500
PciI A'CATG_T
Cuts at: 3876 3876
Size: 5420
PflMI CCAn_nnn'nTGG
Cuts at: 1584 1584
Size: 5420
PfoI T'CCnGG_A
Cuts at: 1211 1370 2303 1211
Size: 159 933 4328
Fragments arranged by size:
4328 933 159
PmeI GTTT'AAAC
Cuts at: 2419 2419
Size: 5420
PmlI CAC'GTG
Cuts at: 726 726
Size: 5420
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4559 4591 4559
Size: 32 5388
PpuMI rG'GwC_Cy
Cuts at: 614 1428 2087 614
Size: 814 659 3947
Fragments arranged by size:
3947 814 659
PshAI GACnn'nnGTC
Cuts at: 766 766
Size: 5420
PspOMI G'GGCC_C
Cuts at: 226 572 2016 226
Size: 346 1444 3630
Fragments arranged by size:
3630 1444 346
PstI C_TGCA'G
Cuts at: 1267 1519 2038 2060 2467 3152 1267
Size: 252 519 22 407 685 3535
Fragments arranged by size:
3535 685 519 407 252 22
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5420
PvuII CAG'CTG
Cuts at: 203 309 399 780 1026 1932 2061 2845 3205
Size: 106 90 381 246 906 129 784 360
Cuts at: 3205 203
Size: 2418
Fragments arranged by size:
2418 906 784 381 360 246 129 106
90
RsrII CG'GwC_CG
Cuts at: 3615 3615
Size: 5420
SacI G_AGCT'C
Cuts at: 2434 2434
Size: 5420
SacII CC_GC'GG
Cuts at: 1864 1864
Size: 5420
SalI G'TCGA_C
Cuts at: 2457 2457
Size: 5420
SapI GCTCTTCn'nnn_
Cuts at: 3443 3653 3443
Size: 210 5210
SbfI CC_TGCA'GG
Cuts at: 2467 2467
Size: 5420
ScaI AGT'ACT
Cuts at: 1705 4937 1705
Size: 3232 2188
SexAI A'CCwGG_T
Cuts at: 238 238
Size: 5420
SfcI C'TryA_G
Cuts at: 33 1263 1515 2034 2056 2463 3148 4141 4332
Size: 1230 252 519 22 407 685 993 191
Cuts at: 4332 33
Size: 1121
Fragments arranged by size:
1230 1121 993 685 519 407 252 191
22
SfoI GGC'GCC
Cuts at: 936 2852 3100 936
Size: 1916 248 3256
Fragments arranged by size:
3256 1916 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5420
SmaI CCC'GGG
Cuts at: 1763 1805 2442 1763
Size: 42 637 4741
Fragments arranged by size:
4741 637 42
SmlI C'TyrA_G
Cuts at: 2335 2575 3490 3982 4244 2335
Size: 240 915 492 262 3511
Fragments arranged by size:
3511 915 492 262 240
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5420
SphI G_CATG'C
Cuts at: 2473 2973 2473
Size: 500 4920
StyI C'CwwG_G
Cuts at: 473 1485 2554 2908 473
Size: 1012 1069 354 2985
Fragments arranged by size:
2985 1069 1012 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 778 2109 3003 3136 3595 3804 778
Size: 1331 894 133 459 209 2394
Fragments arranged by size:
2394 1331 894 459 209 133
TatI w'GTAC_w
Cuts at: 494 983 1703 1979 2309 4935 494
Size: 489 720 276 330 2626 979
Fragments arranged by size:
2626 979 720 489 330 276
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 682 714 1533 1565 4937 4969 682
Size: 32 819 32 3372 32 1133
Fragments arranged by size:
3372 1133 819 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1642 3217 1642
Size: 1575 3845
XbaI T'CTAG_A
Cuts at: 2451 2451
Size: 5420
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1128 1209 2915 1128
Size: 81 1706 3633
Fragments arranged by size:
3633 1706 81
XmaI C'CCGG_G
Cuts at: 1761 1803 2440 1761
Size: 42 637 4741
Fragments arranged by size:
4741 637 42
ZraI GAC'GTC
Cuts at: 1677 1677
Size: 5420
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AgeI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NsiI NspI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PspOMI
PstI PvuI PvuII RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI ZraI
Enzymes that do not cut:
AflII AhdI AleI AloI AscI AvrII BaeI BbvCI
BmgBI BmtI BsiWI BsmBI BssHII BstZ17I ClaI CspCI
EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI
NotI NruI PacI PasI PsiI PspXI PsrI SanDI
SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI
XmnI