Restriction map for
pF1KB5777
(Circular) (Six-base) MAPSORT of: pF1KB5777.seq Check: 6687 from: 1 to: 5579
pF1KB5777 5579 bp
With 182 enzymes: SgfI *
October 7, 2009 14:17 ..
AccI GT'mk_AC
Cuts at: 2617 2617
Size: 5579
Acc65I G'GTAC_C
Cuts at: 2595 2595
Size: 5579
AclI AA'CG_TT
Cuts at: 5419 5419
Size: 5579
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 191 3404 3836 4583 4851 191
Size: 3213 432 747 268 919
Fragments arranged by size:
3213 919 747 432 268
AflII C'TTAA_G
Cuts at: 564 564
Size: 5579
AflIII A'CryG_T
Cuts at: 2931 4035 5012 2931
Size: 1104 977 3498
Fragments arranged by size:
3498 1104 977
AgeI A'CCGG_T
Cuts at: 3967 3967
Size: 5579
AhdI GACnn_n'nnGTC
Cuts at: 400 400
Size: 5579
AleI CACnn'nnGTG
Cuts at: 1156 1666 1156
Size: 510 5069
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1388 1420 1388
Size: 32 5547
AlwNI CAG_nnn'CTG
Cuts at: 1482 2119 2884 4451 1482
Size: 637 765 1567 2610
Fragments arranged by size:
2610 1567 765 637
ApaLI G'TGCA_C
Cuts at: 4349 4349
Size: 5579
ApoI r'AATT_y
Cuts at: 340 648 851 869 1291 2260 2583 4932 5188
Size: 308 203 18 422 969 323 2349 256
Cuts at: 5188 340
Size: 731
Fragments arranged by size:
2349 969 731 422 323 308 256 203
18
AseI AT'TA_AT
Cuts at: 20 20
Size: 5579
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5579
AvaI C'yCGr_G
Cuts at: 2599 2599
Size: 5579
BamHI G'GATC_C
Cuts at: 2604 2604
Size: 5579
BanI G'GyrC_C
Cuts at: 2595 3009 3257 3292 2595
Size: 414 248 35 4882
Fragments arranged by size:
4882 414 248 35
BanII G_rGCy'C
Cuts at: 499 2521 2593 3623 499
Size: 2022 72 1030 2455
Fragments arranged by size:
2455 2022 1030 72
BbeI G_GCGC'C
Cuts at: 3013 3261 3013
Size: 248 5331
BbsI GAAGACnn'nnnn_
Cuts at: 236 1114 236
Size: 878 4701
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 438 472 90
Size: 34 314 34 5197
Fragments arranged by size:
5197 314 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3477 4238 5579 3477
Size: 761 1341 3477
Fragments arranged by size:
3477 1341 761
BclI T'GATC_A
Cuts at: 3099 3099
Size: 5579
BfrBI ATG'CAT
Cuts at: 1461 1461
Size: 5579
BglII A'GATC_T
Cuts at: 516 2532 3094 5131 516
Size: 2016 562 2037 964
Fragments arranged by size:
2037 2016 964 562
BlpI GC'TnA_GC
Cuts at: 2520 2691 2520
Size: 171 5408
Bme1580I G_kGCm'C
Cuts at: 758 3204 3297 4353 758
Size: 2446 93 1056 1984
Fragments arranged by size:
2446 1984 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 2285 2791 2872 3207 5351 2285
Size: 506 81 335 2144 2513
Fragments arranged by size:
2513 2144 506 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3946 3946
Size: 5579
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1768 2096 1768
Size: 328 5251
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 304 1870 2755 3634 4126 4424 304
Size: 1566 885 879 492 298 1459
Fragments arranged by size:
1566 1459 885 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1933 5283 1933
Size: 3350 2229
BsaAI yAC'GTr
Cuts at: 2932 2932
Size: 5579
BsaBI GATnn'nnATC
Cuts at: 6 404 440 3093 5130 6
Size: 398 36 2653 2037 455
Fragments arranged by size:
2653 2037 455 398 36
BsaHI Gr'CG_yC
Cuts at: 3010 3258 3960 5456 3010
Size: 248 702 1496 3133
Fragments arranged by size:
3133 1496 702 248
BsaWI w'CCGG_w
Cuts at: 140 149 209 793 2768 3289 3967 4241 4388
Size: 9 60 584 1975 521 678 274 147
Cuts at: 4388 4799 140
Size: 411 920
Fragments arranged by size:
1975 920 678 584 521 411 274 147
60 9
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 2094 2124 2094
Size: 30 5549
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 489 688 489
Size: 199 5380
BseYI C'CCAG_C
Cuts at: 282 2085 2153 2418 3004 3762 4339 4835 282
Size: 1803 68 265 586 758 577 496 1026
Fragments arranged by size:
1803 1026 758 586 577 496 265 68
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 531 1924 531
Size: 1393 4186
BsiEI CG_ry'CG
Cuts at: 104 3121 3167 4375 104
Size: 3017 46 1208 1308
Fragments arranged by size:
3017 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 2112 2593 3371 3561 4353 2112
Size: 481 778 190 792 3338
Fragments arranged by size:
3338 792 778 481 190
BsmI GAATG_Cn'
Cuts at: 1862 2492 1862
Size: 630 4949
Bsp1286I G_dGCh'C
Cuts at: 499 758 2112 2521 2593 3204 3297 3371 3561
Size: 259 1354 409 72 611 93 74 190
Cuts at: 3561 3623 4353 499
Size: 62 730 1725
Fragments arranged by size:
1725 1354 730 611 409 259 190 93
74 72 62
BspEI T'CCGG_A
Cuts at: 140 149 793 4799 140
Size: 9 644 4006 920
Fragments arranged by size:
4006 920 644 9
BspHI T'CATG_A
Cuts at: 413 4755 413
Size: 4342 1237
BspMI ACCTGCnnnn'nnnn_
Cuts at: 834 2629 2843 3145 3526 834
Size: 1795 214 302 381 2887
Fragments arranged by size:
2887 1795 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3871 3925 5409 5574 50
Size: 3821 54 1484 165 55
Fragments arranged by size:
3821 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 380 1208 2531 3491 380
Size: 828 1323 960 2468
Fragments arranged by size:
2468 1323 960 828
BsrFI r'CCGG_y
Cuts at: 3577 3758 3967 3577
Size: 181 209 5189
Fragments arranged by size:
5189 209 181
BsrGI T'GTAC_A
Cuts at: 1113 1484 1113
Size: 371 5208
BssSI C'ACGA_G
Cuts at: 1673 3850 4208 1673
Size: 2177 358 3044
Fragments arranged by size:
3044 2177 358
BstAPI GCAn_nnn'nTGC
Cuts at: 586 928 1306 2481 2956 586
Size: 342 378 1175 475 3209
Fragments arranged by size:
3209 1175 475 378 342
BstBI TT'CG_AA
Cuts at: 3940 3940
Size: 5579
BstYI r'GATC_y
Cuts at: 1 516 2532 2604 3071 3094 3429 3675 4687
Size: 515 2016 72 467 23 335 246 1012
Cuts at: 4687 4773 4785 5131 1
Size: 86 12 346 449
Fragments arranged by size:
2016 1012 515 467 449 346 335 246
86 72 23 12
BtgI C'CryG_G
Cuts at: 669 669
Size: 5579
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3529 3710 3873 3529
Size: 181 163 5235
Fragments arranged by size:
5235 181 163
BtsI GCAGTG_nn'
Cuts at: 1688 2822 1688
Size: 1134 4445
DraI TTT'AAA
Cuts at: 776 1296 2578 776
Size: 520 1282 3777
Fragments arranged by size:
3777 1282 520
DraIII CAC_nnn'GTG
Cuts at: 586 586
Size: 5579
DrdI GACnn_nn'nnGTC
Cuts at: 3285 4143 3285
Size: 858 4721
EaeI y'GGCC_r
Cuts at: 1575 3164 3338 3729 3756 5101 1575
Size: 1589 174 391 27 1345 2053
Fragments arranged by size:
2053 1589 1345 391 174 27
EagI C'GGCC_G
Cuts at: 3164 3164
Size: 5579
EarI CTCTTCn'nnn_
Cuts at: 1580 1586 2200 3602 3812 1580
Size: 6 614 1402 210 3347
Fragments arranged by size:
3347 1402 614 210 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3787 4097 4243 5389 3787
Size: 310 146 1146 3977
Fragments arranged by size:
3977 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2591 2591
Size: 5579
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 191 1768 2096 3404 3836 4583 4851 191
Size: 1577 328 1308 432 747 268 919
Fragments arranged by size:
1577 1308 919 747 432 328 268
EcoO109I rG'GnC_Cy
Cuts at: 2718 2718
Size: 5579
EcoRI G'AATT_C
Cuts at: 2260 2583 2260
Size: 323 5256
EcoRV GAT'ATC
Cuts at: 2150 2150
Size: 5579
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1279 1513 2265 3598 1279
Size: 234 752 1333 3260
Fragments arranged by size:
3260 1333 752 234
FspI TGC'GCA
Cuts at: 737 3360 737
Size: 2623 2956
HaeII r_GCGC'y
Cuts at: 1482 3013 3261 4283 5262 1482
Size: 1531 248 1022 979 1799
Fragments arranged by size:
1799 1531 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 170 202 386 418 991 1010 1023 1042 1294
Size: 32 184 32 573 19 13 19 252
Cuts at: 1294 1326 3421 3451 3453 3483 170
Size: 32 2095 30 2 30 2266
Fragments arranged by size:
2266 2095 573 252 184 32 32 32
30 30 19 19 13 2
HincII GTy'rAC
Cuts at: 2618 2618
Size: 5579
HindIII A'AGCT_T
Cuts at: 322 748 1702 2497 322
Size: 426 954 795 3404
Fragments arranged by size:
3404 954 795 426
KasI G'GCGC_C
Cuts at: 3009 3257 3009
Size: 248 5331
KpnI G_GTAC'C
Cuts at: 2599 2599
Size: 5579
MfeI C'AATT_G
Cuts at: 1154 1154
Size: 5579
MluI A'CGCG_T
Cuts at: 5012 5012
Size: 5579
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 229 290 578 1604 2056 2150 2345 4250 4434
Size: 61 288 1026 452 94 195 1905 184
Cuts at: 4434 229
Size: 1374
Fragments arranged by size:
1905 1374 1026 452 288 195 184 94
61
MscI TGG'CCA
Cuts at: 1577 3340 1577
Size: 1763 3816
MslI CAynn'nnrTG
Cuts at: 243 706 1156 1372 1666 1774 3568 3695 243
Size: 463 450 216 294 108 1794 127 2127
Fragments arranged by size:
2127 1794 463 450 294 216 127 108
MspA1I CmG'CkG
Cuts at: 2116 2385 2481 2690 3004 3364 4377 4622 2116
Size: 269 96 209 314 360 1013 245 3073
Fragments arranged by size:
3073 1013 360 314 269 245 209 96
NaeI GCC'GGC
Cuts at: 3760 3760
Size: 5579
NarI GG'CG_CC
Cuts at: 3010 3258 3010
Size: 248 5331
NcoI C'CATG_G
Cuts at: 669 669
Size: 5579
NgoMIV G'CCGG_C
Cuts at: 3758 3758
Size: 5579
NsiI A_TGCA'T
Cuts at: 1463 1463
Size: 5579
NspI r_CATG'y
Cuts at: 282 732 1465 2632 3132 4039 282
Size: 450 733 1167 500 907 1822
Fragments arranged by size:
1822 1167 907 733 500 450
PciI A'CATG_T
Cuts at: 4035 4035
Size: 5579
PflMI CCAn_nnn'nTGG
Cuts at: 1355 1957 1355
Size: 602 4977
PmeI GTTT'AAAC
Cuts at: 2578 2578
Size: 5579
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4718 4750 4718
Size: 32 5547
PstI C_TGCA'G
Cuts at: 1332 2214 2367 2390 2626 3311 1332
Size: 882 153 23 236 685 3600
Fragments arranged by size:
3600 882 685 236 153 23
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5579
PvuII CAG'CTG
Cuts at: 2116 2385 2481 3004 3364 2116
Size: 269 96 523 360 4331
Fragments arranged by size:
4331 523 360 269 96
RsrII CG'GwC_CG
Cuts at: 3774 3774
Size: 5579
SacI G_AGCT'C
Cuts at: 2593 2593
Size: 5579
SalI G'TCGA_C
Cuts at: 2616 2616
Size: 5579
SapI GCTCTTCn'nnn_
Cuts at: 1586 3602 3812 1586
Size: 2016 210 3353
Fragments arranged by size:
3353 2016 210
SbfI CC_TGCA'GG
Cuts at: 2626 2626
Size: 5579
ScaI AGT'ACT
Cuts at: 5096 5096
Size: 5579
SfcI C'TryA_G
Cuts at: 33 689 1328 2210 2363 2386 2622 3307 4300
Size: 656 639 882 153 23 236 685 993
Cuts at: 4300 4491 33
Size: 191 1121
Fragments arranged by size:
1121 993 882 685 656 639 236 191
153 23
SfoI GGC'GCC
Cuts at: 3011 3259 3011
Size: 248 5331
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5579
SmaI CCC'GGG
Cuts at: 2601 2601
Size: 5579
SmlI C'TyrA_G
Cuts at: 319 564 1849 2734 3649 4141 4403 319
Size: 245 1285 885 915 492 262 1495
Fragments arranged by size:
1495 1285 915 885 492 262 245
SpeI A'CTAG_T
Cuts at: 67 946 67
Size: 879 4700
SphI G_CATG'C
Cuts at: 1465 2632 3132 1465
Size: 1167 500 3912
Fragments arranged by size:
3912 1167 500
SspI AAT'ATT
Cuts at: 1077 1077
Size: 5579
StuI AGG'CCT
Cuts at: 2317 2317
Size: 5579
StyI C'CwwG_G
Cuts at: 669 2713 3067 669
Size: 2044 354 3181
Fragments arranged by size:
3181 2044 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 3162 3295 3754 3963 3162
Size: 133 459 209 4778
Fragments arranged by size:
4778 459 209 133
TatI w'GTAC_w
Cuts at: 1113 1134 1484 1723 5094 1113
Size: 21 350 239 3371 1598
Fragments arranged by size:
3371 1598 350 239 21
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5096 5128 5096
Size: 32 5547
Tth111I GACn'n_nGTC
Cuts at: 3376 3376
Size: 5579
XbaI T'CTAG_A
Cuts at: 2610 2610
Size: 5579
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1041 1989 3074 1041
Size: 948 1085 3546
Fragments arranged by size:
3546 1085 948
XmaI C'CCGG_G
Cuts at: 2599 2599
Size: 5579
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI
AleI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BcgI BciVI BclI
BfrBI BglII BlpI Bme1580I BmrI BplI BpmI BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstYI
BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII HindIII KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NsiI NspI PciI PflMI PmeI PpiI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AarI AatII AfeI AlfI ApaI AscI AvrII BaeI
BbvCI BglI BmgBI BmtI Bpu10I BsiWI BsmBI BssHII
BstEII BstXI BstZ17I Bsu36I ClaI CspCI EcoNI FseI
FspAI HpaI NdeI NheI NotI NruI PacI PasI
PfoI PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SwaI
XhoI XmnI ZraI