Restriction map for
pF1KB5775
(Circular) (Six-base) MAPSORT of: pF1KB5775.seq Check: 6581 from: 1 to: 5552
pF1KB5775 5552 bp
With 182 enzymes: SgfI *
April 24, 2009 13:05 ..
AccI GT'mk_AC
Cuts at: 2590 2590
Size: 5552
Acc65I G'GTAC_C
Cuts at: 2568 2568
Size: 5552
AclI AA'CG_TT
Cuts at: 5392 5392
Size: 5552
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 380 406 448 476 2048 3377 3809 4556 4824
Size: 26 42 28 1572 1329 432 747 268
Cuts at: 4824 380
Size: 1108
Fragments arranged by size:
1572 1329 1108 747 432 268 42 28
26
AflII C'TTAA_G
Cuts at: 343 1849 343
Size: 1506 4046
AflIII A'CryG_T
Cuts at: 1549 2904 4008 4985 1549
Size: 1355 1104 977 2116
Fragments arranged by size:
2116 1355 1104 977
AgeI A'CCGG_T
Cuts at: 3940 3940
Size: 5552
AlwNI CAG_nnn'CTG
Cuts at: 138 2857 4424 138
Size: 2719 1567 1266
Fragments arranged by size:
2719 1567 1266
ApaLI G'TGCA_C
Cuts at: 4322 4322
Size: 5552
ApoI r'AATT_y
Cuts at: 703 1329 1612 2534 2556 4905 5161 703
Size: 626 283 922 22 2349 256 1094
Fragments arranged by size:
2349 1094 922 626 283 256 22
AseI AT'TA_AT
Cuts at: 20 20
Size: 5552
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5552
AvaI C'yCGr_G
Cuts at: 253 2572 253
Size: 2319 3233
BamHI G'GATC_C
Cuts at: 2577 2577
Size: 5552
BanI G'GyrC_C
Cuts at: 249 302 936 2568 2982 3230 3265 249
Size: 53 634 1632 414 248 35 2536
Fragments arranged by size:
2536 1632 634 414 248 53 35
BanII G_rGCy'C
Cuts at: 239 2566 3596 239
Size: 2327 1030 2195
Fragments arranged by size:
2327 2195 1030
BbeI G_GCGC'C
Cuts at: 253 306 2986 3234 253
Size: 53 2680 248 2571
Fragments arranged by size:
2680 2571 248 53
BbsI GAAGACnn'nnnn_
Cuts at: 2115 2251 2115
Size: 136 5416
BciVI GTATCCnnnnn_n'
Cuts at: 3450 4211 5552 3450
Size: 761 1341 3450
Fragments arranged by size:
3450 1341 761
BclI T'GATC_A
Cuts at: 1910 3072 1910
Size: 1162 4390
BfrBI ATG'CAT
Cuts at: 2226 2428 2226
Size: 202 5350
BglI GCCn_nnn'nGGC
Cuts at: 181 181
Size: 5552
BglII A'GATC_T
Cuts at: 3067 5104 3067
Size: 2037 3515
BlpI GC'TnA_GC
Cuts at: 229 2664 229
Size: 2435 3117
Bme1580I G_kGCm'C
Cuts at: 3177 3270 4326 3177
Size: 93 1056 4403
Fragments arranged by size:
4403 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 2764 2845 3180 5324 2764
Size: 81 335 2144 2992
Fragments arranged by size:
2992 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3919 3919
Size: 5552
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 288 349 373 2405 288
Size: 61 24 2032 3435
Fragments arranged by size:
3435 2032 61 24
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2728 3607 4099 4397 2728
Size: 879 492 298 3883
Fragments arranged by size:
3883 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 910 5256 910
Size: 4346 1206
BsaAI yAC'GTr
Cuts at: 1550 2905 1550
Size: 1355 4197
BsaBI GATnn'nnATC
Cuts at: 6 1997 3066 5103 6
Size: 1991 1069 2037 455
Fragments arranged by size:
2037 1991 1069 455
BsaHI Gr'CG_yC
Cuts at: 250 303 770 2983 3231 3933 5429 250
Size: 53 467 2213 248 702 1496 373
Fragments arranged by size:
2213 1496 702 467 373 248 53
BsaWI w'CCGG_w
Cuts at: 1941 2741 3262 3940 4214 4361 4772 1941
Size: 800 521 678 274 147 411 2721
Fragments arranged by size:
2721 800 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 285 2324 285
Size: 2039 3513
BseYI C'CCAG_C
Cuts at: 370 2977 3735 4312 4808 370
Size: 2607 758 577 496 1114
Fragments arranged by size:
2607 1114 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 152 185 3094 3140 4348 104
Size: 48 33 2909 46 1208 1308
Fragments arranged by size:
2909 1308 1208 48 46 33
BsiHKAI G_wGCw'C
Cuts at: 239 1142 1752 2566 3344 3534 4326 239
Size: 903 610 814 778 190 792 1465
Fragments arranged by size:
1465 903 814 792 778 610 190
BsmI GAATG_Cn'
Cuts at: 1581 1581
Size: 5552
BsmBI CGTCTCn'nnnn_
Cuts at: 777 777
Size: 5552
Bsp1286I G_dGCh'C
Cuts at: 239 1142 1752 2566 3177 3270 3344 3534 3596
Size: 903 610 814 611 93 74 190 62
Cuts at: 3596 4326 239
Size: 730 1465
Fragments arranged by size:
1465 903 814 730 611 610 190 93
74 62
BspEI T'CCGG_A
Cuts at: 4772 4772
Size: 5552
BspHI T'CATG_A
Cuts at: 1824 1907 4728 1824
Size: 83 2821 2648
Fragments arranged by size:
2821 2648 83
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2602 2816 3118 3499 2602
Size: 214 302 381 4655
Fragments arranged by size:
4655 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3844 3898 5382 5547 50
Size: 3794 54 1484 165 55
Fragments arranged by size:
3794 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2070 2207 2329 2406 3464 2070
Size: 137 122 77 1058 4158
Fragments arranged by size:
4158 1058 137 122 77
BsrFI r'CCGG_y
Cuts at: 184 3550 3731 3940 184
Size: 3366 181 209 1796
Fragments arranged by size:
3366 1796 209 181
BsrGI T'GTAC_A
Cuts at: 1398 1398
Size: 5552
BssSI C'ACGA_G
Cuts at: 3823 4181 3823
Size: 358 5194
BstAPI GCAn_nnn'nTGC
Cuts at: 2929 2929
Size: 5552
BstBI TT'CG_AA
Cuts at: 1725 3913 1725
Size: 2188 3364
BstXI CCAn_nnnn'nTGG
Cuts at: 1585 1585
Size: 5552
BstYI r'GATC_y
Cuts at: 1 1227 2058 2577 3044 3067 3402 3648 4660
Size: 1226 831 519 467 23 335 246 1012
Cuts at: 4660 4746 4758 5104 1
Size: 86 12 346 449
Fragments arranged by size:
1226 1012 831 519 467 449 346 335
246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 2304 2304
Size: 5552
BtgI C'CryG_G
Cuts at: 158 2346 158
Size: 2188 3364
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3502 3683 3846 3502
Size: 181 163 5208
Fragments arranged by size:
5208 181 163
BtsI GCAGTG_nn'
Cuts at: 340 650 2095 2795 340
Size: 310 1445 700 3097
Fragments arranged by size:
3097 1445 700 310
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 565 600 565
Size: 35 5517
DraI TTT'AAA
Cuts at: 1619 2551 1619
Size: 932 4620
DrdI GACnn_nn'nnGTC
Cuts at: 3258 4116 3258
Size: 858 4694
EaeI y'GGCC_r
Cuts at: 149 182 3137 3311 3702 3729 5074 149
Size: 33 2955 174 391 27 1345 627
Fragments arranged by size:
2955 1345 627 391 174 33 27
EagI C'GGCC_G
Cuts at: 149 182 3137 149
Size: 33 2955 2564
Fragments arranged by size:
2955 2564 33
EarI CTCTTCn'nnn_
Cuts at: 1738 3575 3785 1738
Size: 1837 210 3505
Fragments arranged by size:
3505 1837 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 295 298 3760 4070 4216 5362 295
Size: 3 3462 310 146 1146 485
Fragments arranged by size:
3462 1146 485 310 146 3
EcoICRI GAG'CTC
Cuts at: 237 2564 237
Size: 2327 3225
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 288 349 373 380 406 448 476 2048 2405
Size: 61 24 7 26 42 28 1572 357
Cuts at: 2405 3377 3809 4556 4824 288
Size: 972 432 747 268 1016
Fragments arranged by size:
1572 1016 972 747 432 357 268 61
42 28 26 24 7
EcoNI CCTnn'n_nnAGG
Cuts at: 244 244
Size: 5552
EcoO109I rG'GnC_Cy
Cuts at: 123 135 2691 123
Size: 12 2556 2984
Fragments arranged by size:
2984 2556 12
EcoRI G'AATT_C
Cuts at: 703 2556 703
Size: 1853 3699
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 537 2264 2268 3571 537
Size: 1727 4 1303 2518
Fragments arranged by size:
2518 1727 1303 4
FspI TGC'GCA
Cuts at: 3333 3333
Size: 5552
HaeII r_GCGC'y
Cuts at: 253 306 2986 3234 4256 5235 253
Size: 53 2680 248 1022 979 570
Fragments arranged by size:
2680 1022 979 570 248 53
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 757 789 3394 3424 3426 3456 757
Size: 32 2605 30 2 30 2853
Fragments arranged by size:
2853 2605 32 30 30 2
HincII GTy'rAC
Cuts at: 2591 2591
Size: 5552
HindIII A'AGCT_T
Cuts at: 1565 1565
Size: 5552
KasI G'GCGC_C
Cuts at: 249 302 2982 3230 249
Size: 53 2680 248 2571
Fragments arranged by size:
2680 2571 248 53
KpnI G_GTAC'C
Cuts at: 2572 2572
Size: 5552
MluI A'CGCG_T
Cuts at: 4985 4985
Size: 5552
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 840 1205 4223 4407 840
Size: 365 3018 184 1985
Fragments arranged by size:
3018 1985 365 184
MscI TGG'CCA
Cuts at: 3313 3313
Size: 5552
MslI CAynn'nnrTG
Cuts at: 1322 3541 3668 1322
Size: 2219 127 3206
Fragments arranged by size:
3206 2219 127
MspA1I CmG'CkG
Cuts at: 168 216 723 1200 1891 2663 2977 3337 4350
Size: 48 507 477 691 772 314 360 1013
Cuts at: 4350 4595 168
Size: 245 1125
Fragments arranged by size:
1125 1013 772 691 507 477 360 314
245 48
NaeI GCC'GGC
Cuts at: 186 3733 186
Size: 3547 2005
NarI GG'CG_CC
Cuts at: 250 303 2983 3231 250
Size: 53 2680 248 2571
Fragments arranged by size:
2680 2571 248 53
NcoI C'CATG_G
Cuts at: 2346 2346
Size: 5552
NgoMIV G'CCGG_C
Cuts at: 184 3731 184
Size: 3547 2005
NsiI A_TGCA'T
Cuts at: 2228 2430 2228
Size: 202 5350
NspI r_CATG'y
Cuts at: 652 2226 2605 3105 4012 652
Size: 1574 379 500 907 2192
Fragments arranged by size:
2192 1574 907 500 379
PciI A'CATG_T
Cuts at: 4008 4008
Size: 5552
PflMI CCAn_nnn'nTGG
Cuts at: 1089 2347 1089
Size: 1258 4294
PfoI T'CCnGG_A
Cuts at: 239 239
Size: 5552
PmeI GTTT'AAAC
Cuts at: 2551 2551
Size: 5552
PmlI CAC'GTG
Cuts at: 1550 1550
Size: 5552
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4691 4723 4691
Size: 32 5520
PpuMI rG'GwC_Cy
Cuts at: 123 123
Size: 5552
PstI C_TGCA'G
Cuts at: 440 2298 2599 3284 440
Size: 1858 301 685 2708
Fragments arranged by size:
2708 1858 685 301
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5552
PvuII CAG'CTG
Cuts at: 723 1200 1891 2977 3337 723
Size: 477 691 1086 360 2938
Fragments arranged by size:
2938 1086 691 477 360
RsrII CG'GwC_CG
Cuts at: 3747 3747
Size: 5552
SacI G_AGCT'C
Cuts at: 239 2566 239
Size: 2327 3225
SalI G'TCGA_C
Cuts at: 2589 2589
Size: 5552
SanDI GG'GwC_CC
Cuts at: 123 123
Size: 5552
SapI GCTCTTCn'nnn_
Cuts at: 3575 3785 3575
Size: 210 5342
SbfI CC_TGCA'GG
Cuts at: 440 2599 440
Size: 2159 3393
ScaI AGT'ACT
Cuts at: 2149 5069 2149
Size: 2920 2632
SfcI C'TryA_G
Cuts at: 33 130 436 1807 2168 2294 2595 3280 4273
Size: 97 306 1371 361 126 301 685 993
Cuts at: 4273 4464 33
Size: 191 1121
Fragments arranged by size:
1371 1121 993 685 361 306 301 191
126 97
SfoI GGC'GCC
Cuts at: 251 304 2984 3232 251
Size: 53 2680 248 2571
Fragments arranged by size:
2680 2571 248 53
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5552
SmaI CCC'GGG
Cuts at: 2574 2574
Size: 5552
SmlI C'TyrA_G
Cuts at: 343 1849 2707 3622 4114 4376 343
Size: 1506 858 915 492 262 1519
Fragments arranged by size:
1519 1506 915 858 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5552
SphI G_CATG'C
Cuts at: 2605 3105 2605
Size: 500 5052
SspI AAT'ATT
Cuts at: 1530 1780 1530
Size: 250 5302
StyI C'CwwG_G
Cuts at: 960 1817 2346 2686 3040 960
Size: 857 529 340 354 3472
Fragments arranged by size:
3472 857 529 354 340
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 385 3135 3268 3727 3936 385
Size: 2750 133 459 209 2001
Fragments arranged by size:
2750 2001 459 209 133
TatI w'GTAC_w
Cuts at: 1398 2147 5067 1398
Size: 749 2920 1883
Fragments arranged by size:
2920 1883 749
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 521 553 1430 1462 5069 5101 521
Size: 32 877 32 3607 32 972
Fragments arranged by size:
3607 972 877 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 3349 3349
Size: 5552
XbaI T'CTAG_A
Cuts at: 1716 1989 2583 1716
Size: 273 594 4685
Fragments arranged by size:
4685 594 273
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 114 1500 2365 3047 114
Size: 1386 865 682 2619
Fragments arranged by size:
2619 1386 865 682
XmaI C'CCGG_G
Cuts at: 2572 2572
Size: 5552
XmnI GAAnn'nnTTC
Cuts at: 461 1342 461
Size: 881 4671
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI
BtsI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NsiI NspI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PstI
PvuI PvuII RsrII SacI SalI SanDI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
SspI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbvCI BcgI BmgBI BmtI Bpu10I
BsaXI BsgI BsiWI BssHII BstEII BstZ17I ClaI DraIII
EcoRV FseI FspAI HpaI MfeI NdeI NheI NotI
NruI PacI PasI PshAI PsiI PspOMI PspXI PsrI
SacII SexAI SfiI SgrAI SnaBI SrfI StuI SwaI
XhoI ZraI