Restriction map for
pF1KB5772
(Circular) (Six-base) MAPSORT of: pF1KB5772.seq Check: 1255 from: 1 to: 5399
pF1KB5772 5399 bp
With 182 enzymes: SgfI *
April 24, 2009 13:05 ..
AccI GT'mk_AC
Cuts at: 741 1601 1685 2437 741
Size: 860 84 752 3703
Fragments arranged by size:
3703 860 752 84
Acc65I G'GTAC_C
Cuts at: 2415 2415
Size: 5399
AclI AA'CG_TT
Cuts at: 5239 5239
Size: 5399
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 373 988 1001 1244 1438 1977 1985 2221 3224
Size: 615 13 243 194 539 8 236 1003
Cuts at: 3224 3656 4403 4671 373
Size: 432 747 268 1101
Fragments arranged by size:
1101 1003 747 615 539 432 268 243
236 194 13 8
AflIII A'CryG_T
Cuts at: 2751 3855 4832 2751
Size: 1104 977 3318
Fragments arranged by size:
3318 1104 977
AgeI A'CCGG_T
Cuts at: 3787 3787
Size: 5399
AlwNI CAG_nnn'CTG
Cuts at: 612 2704 4271 612
Size: 2092 1567 1740
Fragments arranged by size:
2092 1740 1567
ApaLI G'TGCA_C
Cuts at: 4169 4169
Size: 5399
ApoI r'AATT_y
Cuts at: 529 972 1768 2018 2403 4752 5008 529
Size: 443 796 250 385 2349 256 920
Fragments arranged by size:
2349 920 796 443 385 256 250
AseI AT'TA_AT
Cuts at: 20 20
Size: 5399
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5399
AvaI C'yCGr_G
Cuts at: 2419 2419
Size: 5399
BamHI G'GATC_C
Cuts at: 624 1616 2424 624
Size: 992 808 3599
Fragments arranged by size:
3599 992 808
BanI G'GyrC_C
Cuts at: 2415 2829 3077 3112 2415
Size: 414 248 35 4702
Fragments arranged by size:
4702 414 248 35
BanII G_rGCy'C
Cuts at: 323 1721 2036 2303 2413 3443 323
Size: 1398 315 267 110 1030 2279
Fragments arranged by size:
2279 1398 1030 315 267 110
BbeI G_GCGC'C
Cuts at: 2833 3081 2833
Size: 248 5151
BbsI GAAGACnn'nnnn_
Cuts at: 436 436
Size: 5399
BbvCI CC'TCA_GC
Cuts at: 629 1865 629
Size: 1236 4163
BciVI GTATCCnnnnn_n'
Cuts at: 969 3297 4058 5399 969
Size: 2328 761 1341 969
Fragments arranged by size:
2328 1341 969 761
BclI T'GATC_A
Cuts at: 1128 2919 1128
Size: 1791 3608
BfrBI ATG'CAT
Cuts at: 656 656
Size: 5399
BglII A'GATC_T
Cuts at: 2914 4951 2914
Size: 2037 3362
BlpI GC'TnA_GC
Cuts at: 488 2511 488
Size: 2023 3376
Bme1580I G_kGCm'C
Cuts at: 876 3024 3117 4173 876
Size: 2148 93 1056 2102
Fragments arranged by size:
2148 2102 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1492 2023 2611 2692 3027 5171 1492
Size: 531 588 81 335 2144 1720
Fragments arranged by size:
2144 1720 588 531 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1650 3766 1650
Size: 2116 3283
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 226 307 383 549 1717 1743 1931 2345 226
Size: 81 76 166 1168 26 188 414 3280
Fragments arranged by size:
3280 1168 414 188 166 81 76 26
Bpu10I CC'TnA_GC
Cuts at: 629 1707 1865 629
Size: 1078 158 4163
Fragments arranged by size:
4163 1078 158
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 436 1486 2287 2575 3454 3946 4244 436
Size: 1050 801 288 879 492 298 1591
Fragments arranged by size:
1591 1050 879 801 492 298 288
BsaI GGTCTCn'nnnn_
Cuts at: 450 5103 450
Size: 4653 746
BsaAI yAC'GTr
Cuts at: 2752 2752
Size: 5399
BsaBI GATnn'nnATC
Cuts at: 6 2913 4950 6
Size: 2907 2037 455
Fragments arranged by size:
2907 2037 455
BsaHI Gr'CG_yC
Cuts at: 2830 3078 3780 5276 2830
Size: 248 702 1496 2953
Fragments arranged by size:
2953 1496 702 248
BsaWI w'CCGG_w
Cuts at: 425 2588 3109 3787 4061 4208 4619 425
Size: 2163 521 678 274 147 411 1205
Fragments arranged by size:
2163 1205 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 199 229 235 265 295 199
Size: 30 6 30 30 5303
Fragments arranged by size:
5303 30 30 30 6
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 234 331 921 1639 234
Size: 97 590 718 3994
Fragments arranged by size:
3994 718 590 97
BseYI C'CCAG_C
Cuts at: 2190 2378 2824 3582 4159 4655 2190
Size: 188 446 758 577 496 2934
Fragments arranged by size:
2934 758 577 496 446 188
BsiEI CG_ry'CG
Cuts at: 104 2941 2987 4195 104
Size: 2837 46 1208 1308
Fragments arranged by size:
2837 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 323 1117 1636 1721 2036 2303 2413 3191 3381
Size: 794 519 85 315 267 110 778 190
Cuts at: 3381 4173 323
Size: 792 1549
Fragments arranged by size:
1549 794 792 778 519 315 267 190
110 85
BsmI GAATG_Cn'
Cuts at: 658 2360 658
Size: 1702 3697
Bsp1286I G_dGCh'C
Cuts at: 323 876 1117 1636 1721 2036 2303 2413 3024
Size: 553 241 519 85 315 267 110 611
Cuts at: 3024 3117 3191 3381 3443 4173 323
Size: 93 74 190 62 730 1549
Fragments arranged by size:
1549 730 611 553 519 315 267 241
190 110 93 85 74 62
BspEI T'CCGG_A
Cuts at: 4619 4619
Size: 5399
BspHI T'CATG_A
Cuts at: 4575 4575
Size: 5399
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1814 2449 2663 2965 3346 1814
Size: 635 214 302 381 3867
Fragments arranged by size:
3867 635 381 302 214
BsrBI CCG'CTC
Cuts at: 50 246 3691 3745 5229 5394 50
Size: 196 3445 54 1484 165 55
Fragments arranged by size:
3445 1484 196 165 55 54
BsrDI GCAATG_nn'
Cuts at: 299 3311 299
Size: 3012 2387
BsrFI r'CCGG_y
Cuts at: 3397 3578 3787 3397
Size: 181 209 5009
Fragments arranged by size:
5009 209 181
BsrGI T'GTAC_A
Cuts at: 826 826
Size: 5399
BssSI C'ACGA_G
Cuts at: 3670 4028 3670
Size: 358 5041
BstAPI GCAn_nnn'nTGC
Cuts at: 2776 2776
Size: 5399
BstBI TT'CG_AA
Cuts at: 3760 3760
Size: 5399
BstEII G'GTnAC_C
Cuts at: 1870 1870
Size: 5399
BstYI r'GATC_y
Cuts at: 1 236 624 1616 2424 2891 2914 3249 3495
Size: 235 388 992 808 467 23 335 246
Cuts at: 3495 4507 4593 4605 4951 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 992 808 467 449 388 346 335
246 235 86 23 12
BstZ17I GTA'TAC
Cuts at: 1686 1686
Size: 5399
Bsu36I CC'TnA_GG
Cuts at: 2144 2144
Size: 5399
BtgI C'CryG_G
Cuts at: 107 107
Size: 5399
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3349 3530 3693 3349
Size: 181 163 5055
Fragments arranged by size:
5055 181 163
BtsI GCAGTG_nn'
Cuts at: 1150 2642 1150
Size: 1492 3907
DraI TTT'AAA
Cuts at: 2398 2398
Size: 5399
DrdI GACnn_nn'nnGTC
Cuts at: 3105 3963 3105
Size: 858 4541
EaeI y'GGCC_r
Cuts at: 296 1430 2984 3158 3549 3576 4921 296
Size: 1134 1554 174 391 27 1345 774
Fragments arranged by size:
1554 1345 1134 774 391 174 27
EagI C'GGCC_G
Cuts at: 2984 2984
Size: 5399
EarI CTCTTCn'nnn_
Cuts at: 1286 1497 1606 3422 3632 1286
Size: 211 109 1816 210 3053
Fragments arranged by size:
3053 1816 211 210 109
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3607 3917 4063 5209 3607
Size: 310 146 1146 3797
Fragments arranged by size:
3797 1146 310 146
EcoICRI GAG'CTC
Cuts at: 321 1719 2034 2301 2411 321
Size: 1398 315 267 110 3309
Fragments arranged by size:
3309 1398 315 267 110
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 226 307 373 383 549 988 1001 1244 1438
Size: 81 66 10 166 439 13 243 194
Cuts at: 1438 1717 1743 1931 1977 1985 2221 2345 3224
Size: 279 26 188 46 8 236 124 879
Cuts at: 3224 3656 4403 4671 226
Size: 432 747 268 954
Fragments arranged by size:
954 879 747 439 432 279 268 243
236 194 188 166 124 81 66 46
26 13 10 8
EcoO109I rG'GnC_Cy
Cuts at: 2308 2538 2308
Size: 230 5169
EcoRI G'AATT_C
Cuts at: 2018 2403 2018
Size: 385 5014
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 430 3418 430
Size: 2988 2411
FspI TGC'GCA
Cuts at: 3180 3180
Size: 5399
HaeII r_GCGC'y
Cuts at: 2833 3081 4103 5082 2833
Size: 248 1022 979 3150
Fragments arranged by size:
3150 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 142 174 265 297 657 689 1028 1060 1189
Size: 32 91 32 360 32 339 32 129
Cuts at: 1189 1221 1993 2025 2356 2388 3241 3271 3273
Size: 32 772 32 331 32 853 30 2
Cuts at: 3273 3303 142
Size: 30 2238
Fragments arranged by size:
2238 853 772 360 339 331 129 91
32 32 32 32 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 742 1578 1887 2067 2438 742
Size: 836 309 180 371 3703
Fragments arranged by size:
3703 836 371 309 180
KasI G'GCGC_C
Cuts at: 2829 3077 2829
Size: 248 5151
KpnI G_GTAC'C
Cuts at: 2419 2419
Size: 5399
MfeI C'AATT_G
Cuts at: 822 822
Size: 5399
MluI A'CGCG_T
Cuts at: 4832 4832
Size: 5399
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2203 4070 4254 2203
Size: 1867 184 3348
Fragments arranged by size:
3348 1867 184
MscI TGG'CCA
Cuts at: 298 1432 3160 298
Size: 1134 1728 2537
Fragments arranged by size:
2537 1728 1134
MslI CAynn'nnrTG
Cuts at: 149 784 3388 3515 149
Size: 635 2604 127 2033
Fragments arranged by size:
2604 2033 635 127
MspA1I CmG'CkG
Cuts at: 279 342 1140 1416 2510 2824 3184 4197 4442
Size: 63 798 276 1094 314 360 1013 245
Cuts at: 4442 279
Size: 1236
Fragments arranged by size:
1236 1094 1013 798 360 314 276 245
63
NaeI GCC'GGC
Cuts at: 3580 3580
Size: 5399
NarI GG'CG_CC
Cuts at: 2830 3078 2830
Size: 248 5151
NcoI C'CATG_G
Cuts at: 107 107
Size: 5399
NdeI CA'TA_TG
Cuts at: 368 368
Size: 5399
NgoMIV G'CCGG_C
Cuts at: 3578 3578
Size: 5399
NsiI A_TGCA'T
Cuts at: 658 658
Size: 5399
NspI r_CATG'y
Cuts at: 660 1244 1357 1600 1692 2452 2952 3859 660
Size: 584 113 243 92 760 500 907 2200
Fragments arranged by size:
2200 907 760 584 500 243 113 92
PciI A'CATG_T
Cuts at: 3855 3855
Size: 5399
PfoI T'CCnGG_A
Cuts at: 239 239
Size: 5399
PmeI GTTT'AAAC
Cuts at: 2398 2398
Size: 5399
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4538 4570 4538
Size: 32 5367
PpuMI rG'GwC_Cy
Cuts at: 2308 2308
Size: 5399
PstI C_TGCA'G
Cuts at: 356 1157 2446 3131 356
Size: 801 1289 685 2624
Fragments arranged by size:
2624 1289 801 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5399
PvuII CAG'CTG
Cuts at: 279 342 1140 1416 2824 3184 279
Size: 63 798 276 1408 360 2494
Fragments arranged by size:
2494 1408 798 360 276 63
RsrII CG'GwC_CG
Cuts at: 3594 3594
Size: 5399
SacI G_AGCT'C
Cuts at: 323 1721 2036 2303 2413 323
Size: 1398 315 267 110 3309
Fragments arranged by size:
3309 1398 315 267 110
SalI G'TCGA_C
Cuts at: 740 2436 740
Size: 1696 3703
SanDI GG'GwC_CC
Cuts at: 2308 2308
Size: 5399
SapI GCTCTTCn'nnn_
Cuts at: 1286 3422 3632 1286
Size: 2136 210 3053
Fragments arranged by size:
3053 2136 210
SbfI CC_TGCA'GG
Cuts at: 2446 2446
Size: 5399
ScaI AGT'ACT
Cuts at: 4916 4916
Size: 5399
SfcI C'TryA_G
Cuts at: 33 352 1153 1827 2442 3127 4120 4311 33
Size: 319 801 674 615 685 993 191 1121
Fragments arranged by size:
1121 993 801 685 674 615 319 191
SfoI GGC'GCC
Cuts at: 2831 3079 2831
Size: 248 5151
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5399
SmaI CCC'GGG
Cuts at: 2421 2421
Size: 5399
SmlI C'TyrA_G
Cuts at: 415 1465 2302 2554 3469 3961 4223 415
Size: 1050 837 252 915 492 262 1591
Fragments arranged by size:
1591 1050 915 837 492 262 252
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5399
SphI G_CATG'C
Cuts at: 1244 2452 2952 1244
Size: 1208 500 3691
Fragments arranged by size:
3691 1208 500
StuI AGG'CCT
Cuts at: 394 1702 394
Size: 1308 4091
StyI C'CwwG_G
Cuts at: 107 756 2533 2887 107
Size: 649 1777 354 2619
Fragments arranged by size:
2619 1777 649 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 161 2982 3115 3574 3783 161
Size: 2821 133 459 209 1777
Fragments arranged by size:
2821 1777 459 209 133
TatI w'GTAC_w
Cuts at: 826 4914 826
Size: 4088 1311
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1923 1955 2009 2041 4916 4948 1923
Size: 32 54 32 2875 32 2374
Fragments arranged by size:
2875 2374 54 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 3196 3196
Size: 5399
XbaI T'CTAG_A
Cuts at: 1857 2430 1857
Size: 573 4826
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2894 2894
Size: 5399
XmaI C'CCGG_G
Cuts at: 2419 2419
Size: 5399
XmnI GAAnn'nnTTC
Cuts at: 117 1409 1970 117
Size: 1292 561 3546
Fragments arranged by size:
3546 1292 561
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BciVI BclI BfrBI BglII BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstAPI BstBI BstEII BstYI BstZ17I Bsu36I BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NsiI NspI
PciI PfoI PmeI PpiI PpuMI PstI PvuI PvuII
RsrII SacI SalI SanDI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BcgI BglI BmgBI BmtI
BsgI BsiWI BsmBI BssHII BstXI ClaI CspCI DraIII
EcoNI EcoRV FseI FspAI HindIII HpaI NheI NotI
NruI PacI PasI PflMI PmlI PshAI PsiI PspOMI
PspXI PsrI SacII SexAI SfiI SgrAI SnaBI SrfI
SspI SwaI XhoI ZraI