Restriction map for
pF1KB5771
(Circular) (Six-base) MAPSORT of: pF1KB5771.seq Check: 9400 from: 1 to: 5312
pF1KB5771 5312 bp
With 182 enzymes: SgfI *
March 2, 2010 14:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 535 1302 535
Size: 767 4545
AccI GT'mk_AC
Cuts at: 856 1028 2350 856
Size: 172 1322 3818
Fragments arranged by size:
3818 1322 172
Acc65I G'GTAC_C
Cuts at: 1045 2328 1045
Size: 1283 4029
AclI AA'CG_TT
Cuts at: 5152 5152
Size: 5312
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 230 1999 3137 3569 4316 4584 230
Size: 1769 1138 432 747 268 958
Fragments arranged by size:
1769 1138 958 747 432 268
AflII C'TTAA_G
Cuts at: 822 822
Size: 5312
AflIII A'CryG_T
Cuts at: 2664 3768 4745 2664
Size: 1104 977 3231
Fragments arranged by size:
3231 1104 977
AgeI A'CCGG_T
Cuts at: 3700 3700
Size: 5312
AhdI GACnn_n'nnGTC
Cuts at: 1641 1641
Size: 5312
AleI CACnn'nnGTG
Cuts at: 1880 1880
Size: 5312
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1123 1123
Size: 5312
AlwNI CAG_nnn'CTG
Cuts at: 1129 2012 2617 4184 1129
Size: 883 605 1567 2257
Fragments arranged by size:
2257 1567 883 605
ApaLI G'TGCA_C
Cuts at: 1081 4082 1081
Size: 3001 2311
ApoI r'AATT_y
Cuts at: 427 2316 4665 4921 427
Size: 1889 2349 256 818
Fragments arranged by size:
2349 1889 818 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5312
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5312
AvaI C'yCGr_G
Cuts at: 1262 1664 1846 2245 2332 1262
Size: 402 182 399 87 4242
Fragments arranged by size:
4242 402 399 182 87
BamHI G'GATC_C
Cuts at: 2337 2337
Size: 5312
BanI G'GyrC_C
Cuts at: 501 1045 1842 2328 2742 2990 3025 501
Size: 544 797 486 414 248 35 2788
Fragments arranged by size:
2788 797 544 486 414 248 35
BanII G_rGCy'C
Cuts at: 2197 2255 2326 3356 2197
Size: 58 71 1030 4153
Fragments arranged by size:
4153 1030 71 58
BbeI G_GCGC'C
Cuts at: 2746 2994 2746
Size: 248 5064
BbsI GAAGACnn'nnnn_
Cuts at: 781 1247 2102 2217 781
Size: 466 855 115 3876
Fragments arranged by size:
3876 855 466 115
BciVI GTATCCnnnnn_n'
Cuts at: 3210 3971 5312 3210
Size: 761 1341 3210
Fragments arranged by size:
3210 1341 761
BclI T'GATC_A
Cuts at: 2832 2832
Size: 5312
BglI GCCn_nnn'nGGC
Cuts at: 1182 1578 1182
Size: 396 4916
BglII A'GATC_T
Cuts at: 767 1916 2827 4864 767
Size: 1149 911 2037 1215
Fragments arranged by size:
2037 1215 1149 911
BlpI GC'TnA_GC
Cuts at: 2424 2424
Size: 5312
Bme1580I G_kGCm'C
Cuts at: 506 1085 2063 2937 3030 4086 506
Size: 579 978 874 93 1056 1732
Fragments arranged by size:
1732 1056 978 874 579 93
BmrI ACTGGGnnnn_n'
Cuts at: 2000 2524 2605 2940 5084 2000
Size: 524 81 335 2144 2228
Fragments arranged by size:
2228 2144 524 335 81
BmtI G_CTAG'C
Cuts at: 1213 1213
Size: 5312
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1121 1548 1641 3679 1121
Size: 427 93 2038 2754
Fragments arranged by size:
2754 2038 427 93
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 498 803 985 1122 1176 498
Size: 305 182 137 54 4634
Fragments arranged by size:
4634 305 182 137 54
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2488 3367 3859 4157 2488
Size: 879 492 298 3643
Fragments arranged by size:
3643 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1224 1700 2064 5016 1224
Size: 476 364 2952 1520
Fragments arranged by size:
2952 1520 476 364
BsaAI yAC'GTr
Cuts at: 860 2201 2665 860
Size: 1341 464 3507
Fragments arranged by size:
3507 1341 464
BsaBI GATnn'nnATC
Cuts at: 6 973 2826 4863 6
Size: 967 1853 2037 455
Fragments arranged by size:
2037 1853 967 455
BsaHI Gr'CG_yC
Cuts at: 2743 2991 3693 5189 2743
Size: 248 702 1496 2866
Fragments arranged by size:
2866 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1862 2501 3022 3700 3974 4121 4532 1862
Size: 639 521 678 274 147 411 2642
Fragments arranged by size:
2642 678 639 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 529 559 652 682 844 874 2152 2182 529
Size: 30 93 30 162 30 1278 30 3659
Fragments arranged by size:
3659 1278 162 93 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 118 201 231 265 1179 1617 1630 2196 2263
Size: 83 30 34 914 438 13 566 67
Cuts at: 2263 118
Size: 3167
Fragments arranged by size:
3167 914 566 438 83 67 34 30
13
BseYI C'CCAG_C
Cuts at: 1250 1482 1600 2173 2271 2276 2737 3495 4072
Size: 232 118 573 98 5 461 758 577
Cuts at: 4072 4568 1250
Size: 496 1994
Fragments arranged by size:
1994 758 577 573 496 461 232 118
98 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 147 1476 1998 147
Size: 1329 522 3461
Fragments arranged by size:
3461 1329 522
BsiEI CG_ry'CG
Cuts at: 104 1821 2854 2900 4108 104
Size: 1717 1033 46 1208 1308
Fragments arranged by size:
1717 1308 1208 1033 46
BsiHKAI G_wGCw'C
Cuts at: 1085 1907 2197 2206 2326 3104 3294 4086 1085
Size: 822 290 9 120 778 190 792 2311
Fragments arranged by size:
2311 822 792 778 290 190 120 9
Bsp1286I G_dGCh'C
Cuts at: 506 1085 1907 2063 2197 2206 2255 2326 2937
Size: 579 822 156 134 9 49 71 611
Cuts at: 2937 3030 3104 3294 3356 4086 506
Size: 93 74 190 62 730 1732
Fragments arranged by size:
1732 822 730 611 579 190 156 134
93 74 71 62 49 9
BspEI T'CCGG_A
Cuts at: 4532 4532
Size: 5312
BspHI T'CATG_A
Cuts at: 2232 4488 2232
Size: 2256 3056
BspMI ACCTGCnnnn'nnnn_
Cuts at: 535 761 1302 1487 1553 1565 2362 2576 2878
Size: 226 541 185 66 12 797 214 302
Cuts at: 2878 3259 535
Size: 381 2588
Fragments arranged by size:
2588 797 541 381 302 226 214 185
66 12
BsrBI CCG'CTC
Cuts at: 50 588 3604 3658 5142 5307 50
Size: 538 3016 54 1484 165 55
Fragments arranged by size:
3016 1484 538 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3224 3224
Size: 5312
BsrFI r'CCGG_y
Cuts at: 345 599 936 3310 3491 3700 345
Size: 254 337 2374 181 209 1957
Fragments arranged by size:
2374 1957 337 254 209 181
BsrGI T'GTAC_A
Cuts at: 677 2027 677
Size: 1350 3962
BssSI C'ACGA_G
Cuts at: 3583 3941 3583
Size: 358 4954
BstAPI GCAn_nnn'nTGC
Cuts at: 342 2058 2689 342
Size: 1716 631 2965
Fragments arranged by size:
2965 1716 631
BstBI TT'CG_AA
Cuts at: 3673 3673
Size: 5312
BstEII G'GTnAC_C
Cuts at: 1987 1987
Size: 5312
BstXI CCAn_nnnn'nTGG
Cuts at: 448 1638 2157 448
Size: 1190 519 3603
Fragments arranged by size:
3603 1190 519
BstYI r'GATC_y
Cuts at: 1 767 1865 1916 2337 2804 2827 3162 3408
Size: 766 1098 51 421 467 23 335 246
Cuts at: 3408 4420 4506 4518 4864 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1098 1012 766 467 449 421 346 335
246 86 51 23 12
BstZ17I GTA'TAC
Cuts at: 857 857
Size: 5312
Bsu36I CC'TnA_GG
Cuts at: 2208 2208
Size: 5312
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3262 3443 3606 3262
Size: 181 163 4968
Fragments arranged by size:
4968 181 163
BtsI GCAGTG_nn'
Cuts at: 347 400 947 2063 2555 347
Size: 53 547 1116 492 3104
Fragments arranged by size:
3104 1116 547 492 53
DraI TTT'AAA
Cuts at: 2311 2311
Size: 5312
DrdI GACnn_nn'nnGTC
Cuts at: 1273 3018 3876 1273
Size: 1745 858 2709
Fragments arranged by size:
2709 1745 858
EaeI y'GGCC_r
Cuts at: 1091 1174 1818 2897 3071 3462 3489 4834 1091
Size: 83 644 1079 174 391 27 1345 1569
Fragments arranged by size:
1569 1345 1079 644 391 174 83 27
EagI C'GGCC_G
Cuts at: 1818 2897 1818
Size: 1079 4233
EarI CTCTTCn'nnn_
Cuts at: 136 249 923 1033 1048 3335 3545 136
Size: 113 674 110 15 2287 210 1903
Fragments arranged by size:
2287 1903 674 210 113 110 15
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2306 3520 3830 3976 5122 2306
Size: 1214 310 146 1146 2496
Fragments arranged by size:
2496 1214 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2195 2324 2195
Size: 129 5183
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 230 498 803 985 1122 1176 1999 3137 3569
Size: 268 305 182 137 54 823 1138 432
Cuts at: 3569 4316 4584 230
Size: 747 268 958
Fragments arranged by size:
1138 958 823 747 432 305 268 268
182 137 54
EcoNI CCTnn'n_nnAGG
Cuts at: 1294 1294
Size: 5312
EcoO109I rG'GnC_Cy
Cuts at: 386 989 1487 1571 1635 2451 386
Size: 603 498 84 64 816 3247
Fragments arranged by size:
3247 816 603 498 84 64
EcoRI G'AATT_C
Cuts at: 427 2316 427
Size: 1889 3423
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1077 3331 1077
Size: 2254 3058
FspI TGC'GCA
Cuts at: 1111 3093 1111
Size: 1982 3330
HaeII r_GCGC'y
Cuts at: 2746 2994 4016 4995 2746
Size: 248 1022 979 3063
Fragments arranged by size:
3063 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 223 226 255 258 1411 1443 1627 1659 1876
Size: 3 29 3 1153 32 184 32 217
Cuts at: 1876 1908 1940 2152 2184 3154 3184 3186 3216
Size: 32 32 212 32 970 30 2 30
Cuts at: 3216 223
Size: 2319
Fragments arranged by size:
2319 1153 970 217 212 184 32 32
32 32 32 30 30 29 3 3
2
HincII GTy'rAC
Cuts at: 908 2351 908
Size: 1443 3869
KasI G'GCGC_C
Cuts at: 2742 2990 2742
Size: 248 5064
KpnI G_GTAC'C
Cuts at: 1049 2332 1049
Size: 1283 4029
MluI A'CGCG_T
Cuts at: 4745 4745
Size: 5312
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1373 2150 3983 4167 1373
Size: 777 1833 184 2518
Fragments arranged by size:
2518 1833 777 184
MscI TGG'CCA
Cuts at: 1093 3073 1093
Size: 1980 3332
MslI CAynn'nnrTG
Cuts at: 487 721 1880 2035 3301 3428 487
Size: 234 1159 155 1266 127 2371
Fragments arranged by size:
2371 1266 1159 234 155 127
MspA1I CmG'CkG
Cuts at: 573 2088 2241 2423 2737 3097 4110 4355 573
Size: 1515 153 182 314 360 1013 245 1530
Fragments arranged by size:
1530 1515 1013 360 314 245 182 153
NaeI GCC'GGC
Cuts at: 347 938 3493 347
Size: 591 2555 2166
Fragments arranged by size:
2555 2166 591
NarI GG'CG_CC
Cuts at: 2743 2991 2743
Size: 248 5064
NgoMIV G'CCGG_C
Cuts at: 345 936 3491 345
Size: 591 2555 2166
Fragments arranged by size:
2555 2166 591
NheI G'CTAG_C
Cuts at: 1209 1209
Size: 5312
NspI r_CATG'y
Cuts at: 340 2056 2365 2865 3772 340
Size: 1716 309 500 907 1880
Fragments arranged by size:
1880 1716 907 500 309
PasI CC'CwG_GG
Cuts at: 645 1290 1576 645
Size: 645 286 4381
Fragments arranged by size:
4381 645 286
PciI A'CATG_T
Cuts at: 3768 3768
Size: 5312
PmeI GTTT'AAAC
Cuts at: 2311 2311
Size: 5312
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4451 4483 4451
Size: 32 5280
PpuMI rG'GwC_Cy
Cuts at: 386 1635 386
Size: 1249 4063
PstI C_TGCA'G
Cuts at: 1562 2359 3044 1562
Size: 797 685 3830
Fragments arranged by size:
3830 797 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5312
PvuII CAG'CTG
Cuts at: 573 2088 2737 3097 573
Size: 1515 649 360 2788
Fragments arranged by size:
2788 1515 649 360
RsrII CG'GwC_CG
Cuts at: 3507 3507
Size: 5312
SacI G_AGCT'C
Cuts at: 2197 2326 2197
Size: 129 5183
SalI G'TCGA_C
Cuts at: 2349 2349
Size: 5312
SanDI GG'GwC_CC
Cuts at: 386 386
Size: 5312
SapI GCTCTTCn'nnn_
Cuts at: 3335 3545 3335
Size: 210 5102
SbfI CC_TGCA'GG
Cuts at: 1562 2359 1562
Size: 797 4515
ScaI AGT'ACT
Cuts at: 2167 4829 2167
Size: 2662 2650
SexAI A'CCwGG_T
Cuts at: 1637 1655 1637
Size: 18 5294
SfcI C'TryA_G
Cuts at: 33 1558 2355 3040 4033 4224 33
Size: 1525 797 685 993 191 1121
Fragments arranged by size:
1525 1121 993 797 685 191
SfoI GGC'GCC
Cuts at: 2744 2992 2744
Size: 248 5064
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5312
SmaI CCC'GGG
Cuts at: 2334 2334
Size: 5312
SmlI C'TyrA_G
Cuts at: 822 2467 3382 3874 4136 822
Size: 1645 915 492 262 1998
Fragments arranged by size:
1998 1645 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5312
SphI G_CATG'C
Cuts at: 340 2056 2365 2865 340
Size: 1716 309 500 2787
Fragments arranged by size:
2787 1716 500 309
StyI C'CwwG_G
Cuts at: 1535 1631 2446 2800 1535
Size: 96 815 354 4047
Fragments arranged by size:
4047 815 354 96
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1008 1888 2285 2895 3028 3487 3696 1008
Size: 880 397 610 133 459 209 2624
Fragments arranged by size:
2624 880 610 459 397 209 133
TatI w'GTAC_w
Cuts at: 677 2027 2165 4827 677
Size: 1350 138 2662 1162
Fragments arranged by size:
2662 1350 1162 138
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 652 684 844 876 4829 4861 652
Size: 32 160 32 3953 32 1103
Fragments arranged by size:
3953 1103 160 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 3109 3109
Size: 5312
XbaI T'CTAG_A
Cuts at: 2343 2343
Size: 5312
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 636 1725 2284 2807 636
Size: 1089 559 523 3141
Fragments arranged by size:
3141 1089 559 523
XmaI C'CCGG_G
Cuts at: 2332 2332
Size: 5312
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BciVI BclI
BglI BglII BlpI Bme1580I BmrI BmtI BplI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstEII
BstXI BstYI BstZ17I Bsu36I BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NgoMIV
NheI NspI PasI PciI PmeI PpiI PpuMI PstI
PvuI PvuII RsrII SacI SalI SanDI SapI SbfI
ScaI SexAI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AatII AfeI AloI ApaI AscI AvrII BaeI BbvCI
BcgI BfrBI BmgBI Bpu10I BsiWI BsmI BsmBI BssHII
BtgI ClaI CspCI DraIII EcoRV FseI FspAI HindIII
HpaI MfeI NcoI NdeI NotI NruI NsiI PacI
PflMI PfoI PmlI PshAI PsiI PspOMI PspXI PsrI
SacII SfiI SgrAI SnaBI SrfI SspI StuI SwaI
XhoI XmnI ZraI