Restriction map for
pF1KB5768
(Circular) (Six-base) MAPSORT of: pF1KB5768.seq Check: 6675 from: 1 to: 5393
pF1KB5768 5393 bp
With 182 enzymes: SgfI *
April 24, 2009 13:04 ..
AccI GT'mk_AC
Cuts at: 2084 2183 2431 2084
Size: 99 248 5046
Fragments arranged by size:
5046 248 99
Acc65I G'GTAC_C
Cuts at: 2409 2409
Size: 5393
AclI AA'CG_TT
Cuts at: 5233 5233
Size: 5393
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 230 259 1059 3218 3650 4397 4665 230
Size: 29 800 2159 432 747 268 958
Fragments arranged by size:
2159 958 800 747 432 268 29
AflIII A'CryG_T
Cuts at: 2075 2745 3849 4826 2075
Size: 670 1104 977 2642
Fragments arranged by size:
2642 1104 977 670
AgeI A'CCGG_T
Cuts at: 3781 3781
Size: 5393
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1518 1518
Size: 5393
AlwNI CAG_nnn'CTG
Cuts at: 2698 4265 2698
Size: 1567 3826
ApaLI G'TGCA_C
Cuts at: 2221 4163 2221
Size: 1942 3451
ApoI r'AATT_y
Cuts at: 523 763 781 1348 1924 2142 2397 4746 5002
Size: 240 18 567 576 218 255 2349 256
Cuts at: 5002 523
Size: 914
Fragments arranged by size:
2349 914 576 567 256 255 240 218
18
AseI AT'TA_AT
Cuts at: 20 20
Size: 5393
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5393
AvaI C'yCGr_G
Cuts at: 2413 2413
Size: 5393
BamHI G'GATC_C
Cuts at: 2418 2418
Size: 5393
BanI G'GyrC_C
Cuts at: 1448 2409 2823 3071 3106 1448
Size: 961 414 248 35 3735
Fragments arranged by size:
3735 961 414 248 35
BanII G_rGCy'C
Cuts at: 2407 3437 2407
Size: 1030 4363
BbeI G_GCGC'C
Cuts at: 2827 3075 2827
Size: 248 5145
BbsI GAAGACnn'nnnn_
Cuts at: 118 1088 1907 118
Size: 970 819 3604
Fragments arranged by size:
3604 970 819
BciVI GTATCCnnnnn_n'
Cuts at: 1718 1981 3291 4052 5393 1718
Size: 263 1310 761 1341 1718
Fragments arranged by size:
1718 1341 1310 761 263
BclI T'GATC_A
Cuts at: 1551 2913 1551
Size: 1362 4031
BglII A'GATC_T
Cuts at: 2908 4945 2908
Size: 2037 3356
BlpI GC'TnA_GC
Cuts at: 2505 2505
Size: 5393
Bme1580I G_kGCm'C
Cuts at: 2225 3018 3111 4167 2225
Size: 793 93 1056 3451
Fragments arranged by size:
3451 1056 793 93
BmrI ACTGGGnnnn_n'
Cuts at: 711 2605 2686 3021 5165 711
Size: 1894 81 335 2144 939
Fragments arranged by size:
2144 1894 939 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3760 3760
Size: 5393
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 893 2036 2043 893
Size: 1143 7 4243
Fragments arranged by size:
4243 1143 7
Bpu10I CC'TnA_GC
Cuts at: 511 511
Size: 5393
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 954 2149 2569 3448 3940 4238 954
Size: 1195 420 879 492 298 2109
Fragments arranged by size:
2109 1195 879 492 420 298
BsaI GGTCTCn'nnnn_
Cuts at: 755 2110 5097 755
Size: 1355 2987 1051
Fragments arranged by size:
2987 1355 1051
BsaAI yAC'GTr
Cuts at: 998 2746 998
Size: 1748 3645
BsaBI GATnn'nnATC
Cuts at: 6 2123 2907 4944 6
Size: 2117 784 2037 455
Fragments arranged by size:
2117 2037 784 455
BsaHI Gr'CG_yC
Cuts at: 2824 3072 3774 5270 2824
Size: 248 702 1496 2947
Fragments arranged by size:
2947 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2582 3103 3781 4055 4202 4613 2582
Size: 521 678 274 147 411 3362
Fragments arranged by size:
3362 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 512 542 1976 2006 512
Size: 30 1434 30 3899
Fragments arranged by size:
3899 1434 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1673 1849 1673
Size: 176 5217
BseYI C'CCAG_C
Cuts at: 1881 2063 2818 3576 4153 4649 1881
Size: 182 755 758 577 496 2625
Fragments arranged by size:
2625 758 755 577 496 182
BsiEI CG_ry'CG
Cuts at: 104 2935 2981 4189 104
Size: 2831 46 1208 1308
Fragments arranged by size:
2831 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 231 2135 2225 2407 3185 3375 4167 231
Size: 1904 90 182 778 190 792 1457
Fragments arranged by size:
1904 1457 792 778 190 182 90
BsmI GAATG_Cn'
Cuts at: 408 408
Size: 5393
Bsp1286I G_dGCh'C
Cuts at: 231 2135 2225 2407 3018 3111 3185 3375 3437
Size: 1904 90 182 611 93 74 190 62
Cuts at: 3437 4167 231
Size: 730 1457
Fragments arranged by size:
1904 1457 730 611 190 182 93 90
74 62
BspEI T'CCGG_A
Cuts at: 4613 4613
Size: 5393
BspHI T'CATG_A
Cuts at: 1536 4569 1536
Size: 3033 2360
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2443 2657 2959 3340 2443
Size: 214 302 381 4496
Fragments arranged by size:
4496 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3685 3739 5223 5388 50
Size: 3635 54 1484 165 55
Fragments arranged by size:
3635 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 522 965 1350 1811 2291 3305 522
Size: 443 385 461 480 1014 2610
Fragments arranged by size:
2610 1014 480 461 443 385
BsrFI r'CCGG_y
Cuts at: 3391 3572 3781 3391
Size: 181 209 5003
Fragments arranged by size:
5003 209 181
BssSI C'ACGA_G
Cuts at: 3664 4022 3664
Size: 358 5035
BstAPI GCAn_nnn'nTGC
Cuts at: 2245 2770 2245
Size: 525 4868
BstBI TT'CG_AA
Cuts at: 3754 3754
Size: 5393
BstYI r'GATC_y
Cuts at: 1 639 2418 2885 2908 3243 3489 4501 4587
Size: 638 1779 467 23 335 246 1012 86
Cuts at: 4587 4599 4945 1
Size: 12 346 449
Fragments arranged by size:
1779 1012 638 467 449 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 2199 2307 2199
Size: 108 5285
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 842 3343 3524 3687 842
Size: 2501 181 163 2548
Fragments arranged by size:
2548 2501 181 163
BtsI GCAGTG_nn'
Cuts at: 1033 1874 2051 2374 2636 1033
Size: 841 177 323 262 3790
Fragments arranged by size:
3790 841 323 262 177
ClaI AT'CG_AT
Cuts at: 908 908
Size: 5393
DraI TTT'AAA
Cuts at: 251 2392 251
Size: 2141 3252
DrdI GACnn_nn'nnGTC
Cuts at: 3099 3957 3099
Size: 858 4535
EaeI y'GGCC_r
Cuts at: 2978 3152 3543 3570 4915 2978
Size: 174 391 27 1345 3456
Fragments arranged by size:
3456 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2978 2978
Size: 5393
EarI CTCTTCn'nnn_
Cuts at: 663 707 3416 3626 663
Size: 44 2709 210 2430
Fragments arranged by size:
2709 2430 210 44
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3601 3911 4057 5203 3601
Size: 310 146 1146 3791
Fragments arranged by size:
3791 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2405 2405
Size: 5393
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 230 259 893 1059 2036 2043 3218 3650 4397
Size: 29 634 166 977 7 1175 432 747
Cuts at: 4397 4665 230
Size: 268 958
Fragments arranged by size:
1175 977 958 747 634 432 268 166
29 7
EcoO109I rG'GnC_Cy
Cuts at: 934 2532 934
Size: 1598 3795
EcoRI G'AATT_C
Cuts at: 2142 2397 2142
Size: 255 5138
EcoRV GAT'ATC
Cuts at: 1781 1781
Size: 5393
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 325 1728 3412 325
Size: 1403 1684 2306
Fragments arranged by size:
2306 1684 1403
FspI TGC'GCA
Cuts at: 3174 3174
Size: 5393
HaeII r_GCGC'y
Cuts at: 2827 3075 4097 5076 2827
Size: 248 1022 979 3144
Fragments arranged by size:
3144 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1665 1697 1770 1802 3235 3265 3267 3297 1665
Size: 32 73 32 1433 30 2 30 3761
Fragments arranged by size:
3761 1433 73 32 32 30 30 2
HincII GTy'rAC
Cuts at: 2432 2432
Size: 5393
HindIII A'AGCT_T
Cuts at: 466 466
Size: 5393
KasI G'GCGC_C
Cuts at: 2823 3071 2823
Size: 248 5145
KpnI G_GTAC'C
Cuts at: 2413 2413
Size: 5393
MluI A'CGCG_T
Cuts at: 2075 4826 2075
Size: 2751 2642
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 4064 4248 4064
Size: 184 5209
MscI TGG'CCA
Cuts at: 3154 3154
Size: 5393
MslI CAynn'nnrTG
Cuts at: 1933 2347 3382 3509 1933
Size: 414 1035 127 3817
Fragments arranged by size:
3817 1035 414 127
MspA1I CmG'CkG
Cuts at: 1614 1915 2063 2504 2818 3178 4191 4436 1614
Size: 301 148 441 314 360 1013 245 2571
Fragments arranged by size:
2571 1013 441 360 314 301 245 148
NaeI GCC'GGC
Cuts at: 3574 3574
Size: 5393
NarI GG'CG_CC
Cuts at: 2824 3072 2824
Size: 248 5145
NdeI CA'TA_TG
Cuts at: 597 774 1966 597
Size: 177 1192 4024
Fragments arranged by size:
4024 1192 177
NgoMIV G'CCGG_C
Cuts at: 3572 3572
Size: 5393
NspI r_CATG'y
Cuts at: 2446 2946 3853 2446
Size: 500 907 3986
Fragments arranged by size:
3986 907 500
PciI A'CATG_T
Cuts at: 3849 3849
Size: 5393
PflMI CCAn_nnn'nTGG
Cuts at: 871 871
Size: 5393
PfoI T'CCnGG_A
Cuts at: 1568 1568
Size: 5393
PmeI GTTT'AAAC
Cuts at: 2392 2392
Size: 5393
PmlI CAC'GTG
Cuts at: 998 998
Size: 5393
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4532 4564 4532
Size: 32 5361
PpuMI rG'GwC_Cy
Cuts at: 934 934
Size: 5393
PsiI TTA'TAA
Cuts at: 269 344 269
Size: 75 5318
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2323 2355 2323
Size: 32 5361
PstI C_TGCA'G
Cuts at: 324 1744 2440 3125 324
Size: 1420 696 685 2592
Fragments arranged by size:
2592 1420 696 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5393
PvuII CAG'CTG
Cuts at: 1614 1915 2063 2818 3178 1614
Size: 301 148 755 360 3829
Fragments arranged by size:
3829 755 360 301 148
RsrII CG'GwC_CG
Cuts at: 3588 3588
Size: 5393
SacI G_AGCT'C
Cuts at: 2407 2407
Size: 5393
SalI G'TCGA_C
Cuts at: 2430 2430
Size: 5393
SapI GCTCTTCn'nnn_
Cuts at: 3416 3626 3416
Size: 210 5183
SbfI CC_TGCA'GG
Cuts at: 2440 2440
Size: 5393
ScaI AGT'ACT
Cuts at: 338 1489 4910 338
Size: 1151 3421 821
Fragments arranged by size:
3421 1151 821
SfcI C'TryA_G
Cuts at: 33 320 1380 1740 2436 3121 4114 4305 33
Size: 287 1060 360 696 685 993 191 1121
Fragments arranged by size:
1121 1060 993 696 685 360 287 191
SfoI GGC'GCC
Cuts at: 2825 3073 2825
Size: 248 5145
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5393
SmaI CCC'GGG
Cuts at: 2415 2415
Size: 5393
SmlI C'TyrA_G
Cuts at: 969 2128 2548 3463 3955 4217 969
Size: 1159 420 915 492 262 2145
Fragments arranged by size:
2145 1159 915 492 420 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5393
SphI G_CATG'C
Cuts at: 2446 2946 2446
Size: 500 4893
SspI AAT'ATT
Cuts at: 348 683 855 1208 1456 2071 2170 348
Size: 335 172 353 248 615 99 3571
Fragments arranged by size:
3571 615 353 335 248 172 99
StyI C'CwwG_G
Cuts at: 1817 1845 2527 2881 1817
Size: 28 682 354 4329
Fragments arranged by size:
4329 682 354 28
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1044 1873 2359 2976 3109 3568 3777 1044
Size: 829 486 617 133 459 209 2660
Fragments arranged by size:
2660 829 617 486 459 209 133
TatI w'GTAC_w
Cuts at: 336 1487 4908 336
Size: 1151 3421 821
Fragments arranged by size:
3421 1151 821
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2299 2331 4910 4942 2299
Size: 32 2579 32 2750
Fragments arranged by size:
2750 2579 32 32
Tth111I GACn'n_nGTC
Cuts at: 3190 3190
Size: 5393
XbaI T'CTAG_A
Cuts at: 447 2424 447
Size: 1977 3416
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2060 2888 2060
Size: 828 4565
XmaI C'CCGG_G
Cuts at: 2413 2413
Size: 5393
XmnI GAAnn'nnTTC
Cuts at: 261 308 261
Size: 47 5346
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstYI BtgI BtgZI BtsI ClaI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII HindIII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NdeI NgoMIV NspI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PsiI PsrI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI SspI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI ApaI
AscI AvrII BaeI BbvCI BcgI BfrBI BglI BmgBI
BmtI BsgI BsiWI BsmBI BsrGI BssHII BstEII BstXI
BstZ17I Bsu36I CspCI DraIII EcoNI FseI FspAI HpaI
MfeI NcoI NheI NotI NruI NsiI PacI PasI
PshAI PspOMI PspXI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI StuI SwaI XhoI ZraI