Restriction map for
pF1KB5749
(Circular) (Six-base) MAPSORT of: pF1KB5749.seq Check: 6537 from: 1 to: 5537
pF1KB5749 5537 bp
With 182 enzymes: SgfI *
October 7, 2009 14:17 ..
AatII G_ACGT'C
Cuts at: 764 764
Size: 5537
AccI GT'mk_AC
Cuts at: 683 1472 2575 683
Size: 789 1103 3645
Fragments arranged by size:
3645 1103 789
Acc65I G'GTAC_C
Cuts at: 115 2553 115
Size: 2438 3099
AclI AA'CG_TT
Cuts at: 5377 5377
Size: 5537
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 858 2220 2435 3362 3794 4541 4809 858
Size: 1362 215 927 432 747 268 1586
Fragments arranged by size:
1586 1362 927 747 432 268 215
AflIII A'CryG_T
Cuts at: 2889 3993 4970 2889
Size: 1104 977 3456
Fragments arranged by size:
3456 1104 977
AgeI A'CCGG_T
Cuts at: 3925 3925
Size: 5537
AhdI GACnn_n'nnGTC
Cuts at: 1347 1347
Size: 5537
AleI CACnn'nnGTG
Cuts at: 601 1112 601
Size: 511 5026
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1077 1077
Size: 5537
AlwNI CAG_nnn'CTG
Cuts at: 1170 2842 4409 1170
Size: 1672 1567 2298
Fragments arranged by size:
2298 1672 1567
ApaLI G'TGCA_C
Cuts at: 4307 4307
Size: 5537
ApoI r'AATT_y
Cuts at: 197 1336 2541 4890 5146 197
Size: 1139 1205 2349 256 588
Fragments arranged by size:
2349 1205 1139 588 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5537
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5537
AvaI C'yCGr_G
Cuts at: 1196 2557 1196
Size: 1361 4176
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 107 140 107
Size: 33 5504
BamHI G'GATC_C
Cuts at: 1575 2562 1575
Size: 987 4550
BanI G'GyrC_C
Cuts at: 115 2553 2967 3215 3250 115
Size: 2438 414 248 35 2402
Fragments arranged by size:
2438 2402 414 248 35
BanII G_rGCy'C
Cuts at: 1225 1921 2444 2551 3581 1225
Size: 696 523 107 1030 3181
Fragments arranged by size:
3181 1030 696 523 107
BbeI G_GCGC'C
Cuts at: 2971 3219 2971
Size: 248 5289
BbsI GAAGACnn'nnnn_
Cuts at: 194 571 2191 194
Size: 377 1620 3540
Fragments arranged by size:
3540 1620 377
BbvCI CC'TCA_GC
Cuts at: 469 941 469
Size: 472 5065
BciVI GTATCCnnnnn_n'
Cuts at: 1036 3435 4196 5537 1036
Size: 2399 761 1341 1036
Fragments arranged by size:
2399 1341 1036 761
BclI T'GATC_A
Cuts at: 224 3057 224
Size: 2833 2704
BglII A'GATC_T
Cuts at: 1595 3052 5089 1595
Size: 1457 2037 2043
Fragments arranged by size:
2043 2037 1457
BlpI GC'TnA_GC
Cuts at: 923 2649 923
Size: 1726 3811
Bme1580I G_kGCm'C
Cuts at: 3162 3255 4311 3162
Size: 93 1056 4388
Fragments arranged by size:
4388 1056 93
BmgBI CAC'GTC
Cuts at: 438 438
Size: 5537
BmrI ACTGGGnnnn_n'
Cuts at: 2749 2830 3165 5309 2749
Size: 81 335 2144 2977
Fragments arranged by size:
2977 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2463 3904 2463
Size: 1441 4096
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 600 1250 1925 2101 600
Size: 650 675 176 4036
Fragments arranged by size:
4036 675 650 176
Bpu10I CC'TnA_GC
Cuts at: 469 941 1580 469
Size: 472 639 4426
Fragments arranged by size:
4426 639 472
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 679 2246 2713 3592 4084 4382 679
Size: 1567 467 879 492 298 1834
Fragments arranged by size:
1834 1567 879 492 467 298
BsaI GGTCTCn'nnnn_
Cuts at: 1082 1136 2290 5241 1082
Size: 54 1154 2951 1378
Fragments arranged by size:
2951 1378 1154 54
BsaAI yAC'GTr
Cuts at: 405 2890 405
Size: 2485 3052
BsaBI GATnn'nnATC
Cuts at: 6 2492 3051 5088 6
Size: 2486 559 2037 455
Fragments arranged by size:
2486 2037 559 455
BsaHI Gr'CG_yC
Cuts at: 761 2968 3216 3918 5414 761
Size: 2207 248 702 1496 884
Fragments arranged by size:
2207 1496 884 702 248
BsaWI w'CCGG_w
Cuts at: 2726 3247 3925 4199 4346 4757 2726
Size: 521 678 274 147 411 3506
Fragments arranged by size:
3506 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 334 409 658 1215 2155 2263 334
Size: 75 249 557 940 108 3608
Fragments arranged by size:
3608 940 557 249 108 75
BseYI C'CCAG_C
Cuts at: 732 2962 3720 4297 4793 732
Size: 2230 758 577 496 1476
Fragments arranged by size:
2230 1476 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1175 1820 1175
Size: 645 4892
BsiEI CG_ry'CG
Cuts at: 104 3079 3125 4333 104
Size: 2975 46 1208 1308
Fragments arranged by size:
2975 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1635 1921 2551 3329 3519 4311 1635
Size: 286 630 778 190 792 2861
Fragments arranged by size:
2861 792 778 630 286 190
BsmI GAATG_Cn'
Cuts at: 1440 1804 2344 1440
Size: 364 540 4633
Fragments arranged by size:
4633 540 364
BsmBI CGTCTCn'nnnn_
Cuts at: 444 444
Size: 5537
Bsp1286I G_dGCh'C
Cuts at: 1225 1635 1921 2444 2551 3162 3255 3329 3519
Size: 410 286 523 107 611 93 74 190
Cuts at: 3519 3581 4311 1225
Size: 62 730 2451
Fragments arranged by size:
2451 730 611 523 410 286 190 107
93 74 62
BspEI T'CCGG_A
Cuts at: 4757 4757
Size: 5537
BspHI T'CATG_A
Cuts at: 1808 4713 1808
Size: 2905 2632
BspMI ACCTGCnnnn'nnnn_
Cuts at: 275 1469 2030 2587 2801 3103 3484 275
Size: 1194 561 557 214 302 381 2328
Fragments arranged by size:
2328 1194 561 557 381 302 214
BsrBI CCG'CTC
Cuts at: 50 752 3829 3883 5367 5532 50
Size: 702 3077 54 1484 165 55
Fragments arranged by size:
3077 1484 702 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1403 1874 3449 1403
Size: 471 1575 3491
Fragments arranged by size:
3491 1575 471
BsrFI r'CCGG_y
Cuts at: 242 296 736 3535 3716 3925 242
Size: 54 440 2799 181 209 1854
Fragments arranged by size:
2799 1854 440 209 181 54
BssSI C'ACGA_G
Cuts at: 3808 4166 3808
Size: 358 5179
BstAPI GCAn_nnn'nTGC
Cuts at: 2914 2914
Size: 5537
BstBI TT'CG_AA
Cuts at: 3898 3898
Size: 5537
BstEII G'GTnAC_C
Cuts at: 802 802
Size: 5537
BstXI CCAn_nnnn'nTGG
Cuts at: 603 1014 1114 1305 603
Size: 411 100 191 4835
Fragments arranged by size:
4835 411 191 100
BstYI r'GATC_y
Cuts at: 1 1575 1595 2459 2562 3029 3052 3387 3633
Size: 1574 20 864 103 467 23 335 246
Cuts at: 3633 4645 4731 4743 5089 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1574 1012 864 467 449 346 335 246
103 86 23 20 12
BtgI C'CryG_G
Cuts at: 107 119 445 1029 1113 1298 107
Size: 12 326 584 84 185 4346
Fragments arranged by size:
4346 584 326 185 84 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1667 3487 3668 3831 1667
Size: 1820 181 163 3373
Fragments arranged by size:
3373 1820 181 163
BtsI GCAGTG_nn'
Cuts at: 543 1187 1452 1528 2780 543
Size: 644 265 76 1252 3300
Fragments arranged by size:
3300 1252 644 265 76
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1857 1892 1857
Size: 35 5502
DraI TTT'AAA
Cuts at: 2536 2536
Size: 5537
DrdI GACnn_nn'nnGTC
Cuts at: 1860 2497 3243 4101 1860
Size: 637 746 858 3296
Fragments arranged by size:
3296 858 746 637
EaeI y'GGCC_r
Cuts at: 738 1004 1215 1688 1829 1865 1979 3122 3296
Size: 266 211 473 141 36 114 1143 174
Cuts at: 3296 3687 3714 5059 738
Size: 391 27 1345 1216
Fragments arranged by size:
1345 1216 1143 473 391 266 211 174
141 114 36 27
EagI C'GGCC_G
Cuts at: 3122 3122
Size: 5537
EarI CTCTTCn'nnn_
Cuts at: 280 1558 1877 2132 2164 2170 2432 2454 3560
Size: 1278 319 255 32 6 262 22 1106
Cuts at: 3560 3770 280
Size: 210 2047
Fragments arranged by size:
2047 1278 1106 319 262 255 210 32
22 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3745 4055 4201 5347 3745
Size: 310 146 1146 3935
Fragments arranged by size:
3935 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1919 2549 1919
Size: 630 4907
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 600 858 1250 1925 2101 2220 2435 3362 3794
Size: 258 392 675 176 119 215 927 432
Cuts at: 3794 4541 4809 600
Size: 747 268 1328
Fragments arranged by size:
1328 927 747 675 432 392 268 258
215 176 119
EcoNI CCTnn'n_nnAGG
Cuts at: 1855 1855
Size: 5537
EcoO109I rG'GnC_Cy
Cuts at: 729 869 2676 729
Size: 140 1807 3590
Fragments arranged by size:
3590 1807 140
EcoRI G'AATT_C
Cuts at: 2541 2541
Size: 5537
EcoRV GAT'ATC
Cuts at: 990 1683 990
Size: 693 4844
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1008 3556 1008
Size: 2548 2989
FspI TGC'GCA
Cuts at: 271 1900 2387 3318 271
Size: 1629 487 931 2490
Fragments arranged by size:
2490 1629 931 487
HaeII r_GCGC'y
Cuts at: 1281 2971 3219 4241 5220 1281
Size: 1690 248 1022 979 1598
Fragments arranged by size:
1690 1598 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1333 1365 2104 2136 3379 3409 3411 3441 1333
Size: 32 739 32 1243 30 2 30 3429
Fragments arranged by size:
3429 1243 739 32 32 30 30 2
HincII GTy'rAC
Cuts at: 2576 2576
Size: 5537
KasI G'GCGC_C
Cuts at: 2967 3215 2967
Size: 248 5289
KpnI G_GTAC'C
Cuts at: 119 2557 119
Size: 2438 3099
MluI A'CGCG_T
Cuts at: 4970 4970
Size: 5537
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 4208 4392 4208
Size: 184 5353
MscI TGG'CCA
Cuts at: 1006 1690 1831 1981 3298 1006
Size: 684 141 150 1317 3245
Fragments arranged by size:
3245 1317 684 150 141
MslI CAynn'nnrTG
Cuts at: 601 1034 1040 1112 1825 3526 3653 601
Size: 433 6 72 713 1701 127 2485
Fragments arranged by size:
2485 1701 713 433 127 72 6
MspA1I CmG'CkG
Cuts at: 339 447 473 1002 1442 1459 2648 2962 3322
Size: 108 26 529 440 17 1189 314 360
Cuts at: 3322 4335 4580 339
Size: 1013 245 1296
Fragments arranged by size:
1296 1189 1013 529 440 360 314 245
108 26 17
NaeI GCC'GGC
Cuts at: 738 3718 738
Size: 2980 2557
NarI GG'CG_CC
Cuts at: 2968 3216 2968
Size: 248 5289
NcoI C'CATG_G
Cuts at: 107 1029 1298 107
Size: 922 269 4346
Fragments arranged by size:
4346 922 269
NgoMIV G'CCGG_C
Cuts at: 736 3716 736
Size: 2980 2557
NspI r_CATG'y
Cuts at: 2590 3090 3997 2590
Size: 500 907 4130
Fragments arranged by size:
4130 907 500
PciI A'CATG_T
Cuts at: 3993 3993
Size: 5537
PflMI CCAn_nnn'nTGG
Cuts at: 2316 2316
Size: 5537
PfoI T'CCnGG_A
Cuts at: 173 710 840 2372 173
Size: 537 130 1532 3338
Fragments arranged by size:
3338 1532 537 130
PmeI GTTT'AAAC
Cuts at: 2536 2536
Size: 5537
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4676 4708 4676
Size: 32 5505
PpuMI rG'GwC_Cy
Cuts at: 869 869
Size: 5537
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 382 414 382
Size: 32 5505
PstI C_TGCA'G
Cuts at: 1447 2027 2584 3269 1447
Size: 580 557 685 3715
Fragments arranged by size:
3715 685 580 557
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5537
PvuII CAG'CTG
Cuts at: 473 1002 1459 2962 3322 473
Size: 529 457 1503 360 2688
Fragments arranged by size:
2688 1503 529 457 360
RsrII CG'GwC_CG
Cuts at: 3732 3732
Size: 5537
SacI G_AGCT'C
Cuts at: 1921 2551 1921
Size: 630 4907
SacII CC_GC'GG
Cuts at: 448 448
Size: 5537
SalI G'TCGA_C
Cuts at: 2574 2574
Size: 5537
SapI GCTCTTCn'nnn_
Cuts at: 1558 2432 3560 3770 1558
Size: 874 1128 210 3325
Fragments arranged by size:
3325 1128 874 210
SbfI CC_TGCA'GG
Cuts at: 2027 2584 2027
Size: 557 4980
ScaI AGT'ACT
Cuts at: 5054 5054
Size: 5537
SfcI C'TryA_G
Cuts at: 33 1443 1519 2023 2580 3265 4258 4449 33
Size: 1410 76 504 557 685 993 191 1121
Fragments arranged by size:
1410 1121 993 685 557 504 191 76
SfoI GGC'GCC
Cuts at: 2969 3217 2969
Size: 248 5289
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5537
SmaI CCC'GGG
Cuts at: 2559 2559
Size: 5537
SmlI C'TyrA_G
Cuts at: 658 2261 2692 3607 4099 4361 658
Size: 1603 431 915 492 262 1834
Fragments arranged by size:
1834 1603 915 492 431 262
SnaBI TAC'GTA
Cuts at: 405 405
Size: 5537
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5537
SphI G_CATG'C
Cuts at: 2590 3090 2590
Size: 500 5037
SspI AAT'ATT
Cuts at: 1128 1128
Size: 5537
StyI C'CwwG_G
Cuts at: 107 635 983 1029 1298 1409 1664 2300 2671
Size: 528 348 46 269 111 255 636 371
Cuts at: 2671 3025 107
Size: 354 2619
Fragments arranged by size:
2619 636 528 371 354 348 269 255
111 46
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1328 1747 3120 3253 3712 3921 1328
Size: 419 1373 133 459 209 2944
Fragments arranged by size:
2944 1373 459 419 209 133
TatI w'GTAC_w
Cuts at: 1817 2265 5052 1817
Size: 448 2787 2302
Fragments arranged by size:
2787 2302 448
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1683 1715 5054 5086 1683
Size: 32 3339 32 2134
Fragments arranged by size:
3339 2134 32 32
Tth111I GACn'n_nGTC
Cuts at: 1042 1606 3334 1042
Size: 564 1728 3245
Fragments arranged by size:
3245 1728 564
XbaI T'CTAG_A
Cuts at: 2568 2568
Size: 5537
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3032 3032
Size: 5537
XmaI C'CCGG_G
Cuts at: 2557 2557
Size: 5537
XmnI GAAnn'nnTTC
Cuts at: 2344 2344
Size: 5537
ZraI GAC'GTC
Cuts at: 762 762
Size: 5537
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AgeI AhdI
AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI
BaeI BamHI BanI BanII BbeI BbsI BbvCI BciVI
BclI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstEII BstXI BstYI BtgI BtgZI BtsI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PciI PflMI PfoI PmeI
PpiI PpuMI PsrI PstI PvuI PvuII RsrII SacI
SacII SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SnaBI SpeI SphI SspI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AflII AloI ApaI AscI AvrII BcgI
BfrBI BglI BmtI BsaXI BsiWI BsrGI BssHII BstZ17I
Bsu36I ClaI DraIII FseI FspAI HindIII HpaI MfeI
NdeI NheI NotI NruI NsiI PacI PasI PmlI
PshAI PsiI PspOMI PspXI SanDI SexAI SfiI SgrAI
SrfI StuI SwaI XhoI