Restriction map for
pF1KB5746
(Circular) (Six-base) MAPSORT of: pF1KB5746.seq Check: 8455 from: 1 to: 5351
pF1KB5746 5351 bp
With 182 enzymes: SgfI *
January 20, 2017 09:25 ..
AccI GT'mk_AC
Cuts at: 1379 1506 2389 1379
Size: 127 883 4341
Fragments arranged by size:
4341 883 127
Acc65I G'GTAC_C
Cuts at: 2367 2367
Size: 5351
AclI AA'CG_TT
Cuts at: 2345 5191 2345
Size: 2846 2505
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 3176 3608 4355 4623 3176
Size: 432 747 268 3904
Fragments arranged by size:
3904 747 432 268
AflIII A'CryG_T
Cuts at: 2703 3807 4784 2703
Size: 1104 977 3270
Fragments arranged by size:
3270 1104 977
AgeI A'CCGG_T
Cuts at: 3739 3739
Size: 5351
AleI CACnn'nnGTG
Cuts at: 700 700
Size: 5351
AlwNI CAG_nnn'CTG
Cuts at: 853 2241 2656 4223 853
Size: 1388 415 1567 1981
Fragments arranged by size:
1981 1567 1388 415
ApaI G_GGCC'C
Cuts at: 745 745
Size: 5351
ApaLI G'TGCA_C
Cuts at: 751 4121 751
Size: 3370 1981
ApoI r'AATT_y
Cuts at: 2355 4704 4960 2355
Size: 2349 256 2746
Fragments arranged by size:
2746 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5351
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5351
AvaI C'yCGr_G
Cuts at: 188 1982 2371 188
Size: 1794 389 3168
Fragments arranged by size:
3168 1794 389
BamHI G'GATC_C
Cuts at: 2376 2376
Size: 5351
BanI G'GyrC_C
Cuts at: 203 2367 2781 3029 3064 203
Size: 2164 414 248 35 2490
Fragments arranged by size:
2490 2164 414 248 35
BanII G_rGCy'C
Cuts at: 549 745 1064 1259 2365 3395 549
Size: 196 319 195 1106 1030 2505
Fragments arranged by size:
2505 1106 1030 319 196 195
BbeI G_GCGC'C
Cuts at: 2785 3033 2785
Size: 248 5103
BbsI GAAGACnn'nnnn_
Cuts at: 1554 1554
Size: 5351
BciVI GTATCCnnnnn_n'
Cuts at: 1181 1798 1989 3249 4010 5351 1181
Size: 617 191 1260 761 1341 1181
Fragments arranged by size:
1341 1260 1181 761 617 191
BclI T'GATC_A
Cuts at: 2871 2871
Size: 5351
BglI GCCn_nnn'nGGC
Cuts at: 967 1216 967
Size: 249 5102
BglII A'GATC_T
Cuts at: 2022 2866 4903 2022
Size: 844 2037 2470
Fragments arranged by size:
2470 2037 844
BlpI GC'TnA_GC
Cuts at: 2463 2463
Size: 5351
Bme1580I G_kGCm'C
Cuts at: 745 755 2976 3069 4125 745
Size: 10 2221 93 1056 1971
Fragments arranged by size:
2221 1971 1056 93 10
BmrI ACTGGGnnnn_n'
Cuts at: 1152 1963 2563 2644 2979 5123 1152
Size: 811 600 81 335 2144 1380
Fragments arranged by size:
2144 1380 811 600 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3718 3718
Size: 5351
Bpu10I CC'TnA_GC
Cuts at: 1638 1638
Size: 5351
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 657 744 2527 3406 3898 4196 657
Size: 87 1783 879 492 298 1812
Fragments arranged by size:
1812 1783 879 492 298 87
BsaI GGTCTCn'nnnn_
Cuts at: 185 1927 5055 185
Size: 1742 3128 481
Fragments arranged by size:
3128 1742 481
BsaAI yAC'GTr
Cuts at: 756 2704 756
Size: 1948 3403
BsaBI GATnn'nnATC
Cuts at: 6 334 2865 4902 6
Size: 328 2531 2037 455
Fragments arranged by size:
2531 2037 455 328
BsaHI Gr'CG_yC
Cuts at: 2782 3030 3732 5228 2782
Size: 248 702 1496 2905
Fragments arranged by size:
2905 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2540 3061 3739 4013 4160 4571 2540
Size: 521 678 274 147 411 3320
Fragments arranged by size:
3320 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 136 1287 2096 136
Size: 1151 809 3391
Fragments arranged by size:
3391 1151 809
BseYI C'CCAG_C
Cuts at: 476 1080 2223 2776 3534 4111 4607 476
Size: 604 1143 553 758 577 496 1220
Fragments arranged by size:
1220 1143 758 604 577 553 496
BsiEI CG_ry'CG
Cuts at: 104 2893 2939 4147 104
Size: 2789 46 1208 1308
Fragments arranged by size:
2789 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 755 879 1064 1110 1249 1259 2365 3143 3333
Size: 124 185 46 139 10 1106 778 190
Cuts at: 3333 4125 755
Size: 792 1981
Fragments arranged by size:
1981 1106 792 778 190 185 139 124
46 10
BsmI GAATG_Cn'
Cuts at: 973 2100 973
Size: 1127 4224
BsmBI CGTCTCn'nnnn_
Cuts at: 1801 1801
Size: 5351
Bsp1286I G_dGCh'C
Cuts at: 549 745 755 879 1064 1110 1249 1259 2365
Size: 196 10 124 185 46 139 10 1106
Cuts at: 2365 2976 3069 3143 3333 3395 4125 549
Size: 611 93 74 190 62 730 1775
Fragments arranged by size:
1775 1106 730 611 196 190 185 139
124 93 74 62 46 10 10
BspEI T'CCGG_A
Cuts at: 4571 4571
Size: 5351
BspHI T'CATG_A
Cuts at: 4527 4527
Size: 5351
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1492 2401 2615 2917 3298 1492
Size: 909 214 302 381 3545
Fragments arranged by size:
3545 909 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3643 3697 5181 5346 50
Size: 3593 54 1484 165 55
Fragments arranged by size:
3593 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1127 1394 1622 1859 2110 3263 1127
Size: 267 228 237 251 1153 3215
Fragments arranged by size:
3215 1153 267 251 237 228
BsrFI r'CCGG_y
Cuts at: 1362 3349 3530 3739 1362
Size: 1987 181 209 2974
Fragments arranged by size:
2974 1987 209 181
BssSI C'ACGA_G
Cuts at: 1063 3622 3980 1063
Size: 2559 358 2434
Fragments arranged by size:
2559 2434 358
BstAPI GCAn_nnn'nTGC
Cuts at: 2728 2728
Size: 5351
BstBI TT'CG_AA
Cuts at: 3712 3712
Size: 5351
BstXI CCAn_nnnn'nTGG
Cuts at: 699 1161 699
Size: 462 4889
BstYI r'GATC_y
Cuts at: 1 1454 2022 2322 2376 2843 2866 3201 3447
Size: 1453 568 300 54 467 23 335 246
Cuts at: 3447 4459 4545 4557 4903 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1453 1012 568 467 449 346 335 300
246 86 54 23 12
BtgI C'CryG_G
Cuts at: 107 1045 2192 107
Size: 938 1147 3266
Fragments arranged by size:
3266 1147 938
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3301 3482 3645 3301
Size: 181 163 5007
Fragments arranged by size:
5007 181 163
BtsI GCAGTG_nn'
Cuts at: 716 786 1205 2242 2594 716
Size: 70 419 1037 352 3473
Fragments arranged by size:
3473 1037 419 352 70
DraI TTT'AAA
Cuts at: 2350 2350
Size: 5351
DraIII CAC_nnn'GTG
Cuts at: 258 258
Size: 5351
DrdI GACnn_nn'nnGTC
Cuts at: 3057 3915 3057
Size: 858 4493
EaeI y'GGCC_r
Cuts at: 2936 3110 3501 3528 4873 2936
Size: 174 391 27 1345 3414
Fragments arranged by size:
3414 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2936 2936
Size: 5351
EarI CTCTTCn'nnn_
Cuts at: 434 902 1823 2013 2344 3374 3584 434
Size: 468 921 190 331 1030 210 2201
Fragments arranged by size:
2201 1030 921 468 331 210 190
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3559 3869 4015 5161 3559
Size: 310 146 1146 3749
Fragments arranged by size:
3749 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1062 1257 2363 1062
Size: 195 1106 4050
Fragments arranged by size:
4050 1106 195
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 3176 3608 4355 4623 3176
Size: 432 747 268 3904
Fragments arranged by size:
3904 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 137 907 1903 137
Size: 770 996 3585
Fragments arranged by size:
3585 996 770
EcoO109I rG'GnC_Cy
Cuts at: 143 741 742 2490 143
Size: 598 1 1748 3004
Fragments arranged by size:
3004 1748 598 1
EcoRI G'AATT_C
Cuts at: 2355 2355
Size: 5351
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3370 3370
Size: 5351
FspI TGC'GCA
Cuts at: 3132 3132
Size: 5351
HaeII r_GCGC'y
Cuts at: 2785 3033 4055 5034 2785
Size: 248 1022 979 3102
Fragments arranged by size:
3102 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 820 852 1650 1682 1866 1898 3193 3223 3225
Size: 32 798 32 184 32 1295 30 2
Cuts at: 3225 3255 820
Size: 30 2916
Fragments arranged by size:
2916 1295 798 184 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 236 1507 1892 2390 236
Size: 1271 385 498 3197
Fragments arranged by size:
3197 1271 498 385
HindIII A'AGCT_T
Cuts at: 2140 2140
Size: 5351
KasI G'GCGC_C
Cuts at: 2781 3029 2781
Size: 248 5103
KpnI G_GTAC'C
Cuts at: 2371 2371
Size: 5351
MfeI C'AATT_G
Cuts at: 164 2036 164
Size: 1872 3479
MluI A'CGCG_T
Cuts at: 4784 4784
Size: 5351
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1120 4022 4206 1120
Size: 2902 184 2265
Fragments arranged by size:
2902 2265 184
MscI TGG'CCA
Cuts at: 3112 3112
Size: 5351
MslI CAynn'nnrTG
Cuts at: 700 1240 3340 3467 700
Size: 540 2100 127 2584
Fragments arranged by size:
2584 2100 540 127
MspA1I CmG'CkG
Cuts at: 853 1398 1533 2462 2776 3136 4149 4394 853
Size: 545 135 929 314 360 1013 245 1810
Fragments arranged by size:
1810 1013 929 545 360 314 245 135
NaeI GCC'GGC
Cuts at: 3532 3532
Size: 5351
NarI GG'CG_CC
Cuts at: 2782 3030 2782
Size: 248 5103
NcoI C'CATG_G
Cuts at: 107 1045 2192 107
Size: 938 1147 3266
Fragments arranged by size:
3266 1147 938
NgoMIV G'CCGG_C
Cuts at: 3530 3530
Size: 5351
NspI r_CATG'y
Cuts at: 430 2404 2904 3811 430
Size: 1974 500 907 1970
Fragments arranged by size:
1974 1970 907 500
PciI A'CATG_T
Cuts at: 3807 3807
Size: 5351
PflMI CCAn_nnn'nTGG
Cuts at: 133 133
Size: 5351
PmeI GTTT'AAAC
Cuts at: 2350 2350
Size: 5351
PmlI CAC'GTG
Cuts at: 756 756
Size: 5351
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4490 4522 4490
Size: 32 5319
PpuMI rG'GwC_Cy
Cuts at: 143 143
Size: 5351
PshAI GACnn'nnGTC
Cuts at: 452 452
Size: 5351
PspOMI G'GGCC_C
Cuts at: 741 741
Size: 5351
PstI C_TGCA'G
Cuts at: 846 1632 1731 2398 3083 846
Size: 786 99 667 685 3114
Fragments arranged by size:
3114 786 685 667 99
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5351
PvuII CAG'CTG
Cuts at: 853 1398 1533 2776 3136 853
Size: 545 135 1243 360 3068
Fragments arranged by size:
3068 1243 545 360 135
RsrII CG'GwC_CG
Cuts at: 3546 3546
Size: 5351
SacI G_AGCT'C
Cuts at: 1064 1259 2365 1064
Size: 195 1106 4050
Fragments arranged by size:
4050 1106 195
SalI G'TCGA_C
Cuts at: 1505 2388 1505
Size: 883 4468
SapI GCTCTTCn'nnn_
Cuts at: 3374 3584 3374
Size: 210 5141
SbfI CC_TGCA'GG
Cuts at: 2398 2398
Size: 5351
ScaI AGT'ACT
Cuts at: 4868 4868
Size: 5351
SfcI C'TryA_G
Cuts at: 33 842 863 881 1380 1496 1628 1727 2394
Size: 809 21 18 499 116 132 99 667
Cuts at: 2394 3079 4072 4263 33
Size: 685 993 191 1121
Fragments arranged by size:
1121 993 809 685 667 499 191 132
116 99 21 18
SfoI GGC'GCC
Cuts at: 2783 3031 2783
Size: 248 5103
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5351
SmaI CCC'GGG
Cuts at: 1984 2373 1984
Size: 389 4962
SmlI C'TyrA_G
Cuts at: 188 636 723 2506 3421 3913 4175 188
Size: 448 87 1783 915 492 262 1364
Fragments arranged by size:
1783 1364 915 492 448 262 87
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5351
SphI G_CATG'C
Cuts at: 2404 2904 2404
Size: 500 4851
StyI C'CwwG_G
Cuts at: 107 698 962 1045 2192 2485 2839 107
Size: 591 264 83 1147 293 354 2619
Fragments arranged by size:
2619 1147 591 354 293 264 83
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 162 1171 1737 2145 2205 2934 3067 3526 3735
Size: 1009 566 408 60 729 133 459 209
Cuts at: 3735 162
Size: 1778
Fragments arranged by size:
1778 1009 729 566 459 408 209 133
60
TatI w'GTAC_w
Cuts at: 4866 4866
Size: 5351
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1100 1132 4868 4900 1100
Size: 32 3736 32 1551
Fragments arranged by size:
3736 1551 32 32
Tth111I GACn'n_nGTC
Cuts at: 3148 3148
Size: 5351
XbaI T'CTAG_A
Cuts at: 2382 2382
Size: 5351
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 114 2846 114
Size: 2732 2619
XhoI C'TCGA_G
Cuts at: 188 188
Size: 5351
XmaI C'CCGG_G
Cuts at: 1982 2371 1982
Size: 389 4962
XmnI GAAnn'nnTTC
Cuts at: 1324 1324
Size: 5351
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BglI BglII BlpI
Bme1580I BmrI BplI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PciI PflMI
PmeI PmlI PpiI PpuMI PshAI PspOMI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI AscI
AvrII BaeI BbvCI BcgI BfrBI BmgBI BmtI BpmI
BsaXI BsgI BsiWI BsrGI BssHII BstEII BstZ17I Bsu36I
ClaI CspCI EcoRV FseI FspAI HpaI NdeI NheI
NotI NruI NsiI PacI PasI PfoI PsiI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
SspI StuI SwaI ZraI