Restriction map for
pF1KB5741
(Circular) (Six-base) MAPSORT of: pF1KB5741.seq Check: 6269 from: 1 to: 5510
pF1KB5741 5510 bp
With 182 enzymes: SgfI *
March 2, 2010 14:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2005 2005
Size: 5510
AatII G_ACGT'C
Cuts at: 425 425
Size: 5510
AccI GT'mk_AC
Cuts at: 2548 2548
Size: 5510
Acc65I G'GTAC_C
Cuts at: 695 2526 695
Size: 1831 3679
AclI AA'CG_TT
Cuts at: 5350 5350
Size: 5510
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1375 3335 3767 4514 4782 1375
Size: 1960 432 747 268 2103
Fragments arranged by size:
2103 1960 747 432 268
AflIII A'CryG_T
Cuts at: 2862 3966 4943 2862
Size: 1104 977 3429
Fragments arranged by size:
3429 1104 977
AgeI A'CCGG_T
Cuts at: 3898 3898
Size: 5510
AleI CACnn'nnGTG
Cuts at: 2110 2122 2194 2110
Size: 12 72 5426
Fragments arranged by size:
5426 72 12
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 897 1448 897
Size: 551 4959
AlwNI CAG_nnn'CTG
Cuts at: 1641 2815 4382 1641
Size: 1174 1567 2769
Fragments arranged by size:
2769 1567 1174
ApaI G_GGCC'C
Cuts at: 120 2400 120
Size: 2280 3230
ApaLI G'TGCA_C
Cuts at: 4280 4280
Size: 5510
ApoI r'AATT_y
Cuts at: 1146 2514 4863 5119 1146
Size: 1368 2349 256 1537
Fragments arranged by size:
2349 1537 1368 256
AseI AT'TA_AT
Cuts at: 20 1223 20
Size: 1203 4307
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5510
AvaI C'yCGr_G
Cuts at: 2530 2530
Size: 5510
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 679 712 1221 1254 679
Size: 33 509 33 4935
Fragments arranged by size:
4935 509 33 33
BamHI G'GATC_C
Cuts at: 2535 2535
Size: 5510
BanI G'GyrC_C
Cuts at: 123 589 687 695 1591 2526 2940 3188 3223
Size: 466 98 8 896 935 414 248 35
Cuts at: 3223 123
Size: 2410
Fragments arranged by size:
2410 935 896 466 414 248 98 35
8
BanII G_rGCy'C
Cuts at: 120 152 352 409 2204 2400 2524 3554 120
Size: 32 200 57 1795 196 124 1030 2076
Fragments arranged by size:
2076 1795 1030 200 196 124 57 32
BbeI G_GCGC'C
Cuts at: 127 2944 3192 127
Size: 2817 248 2445
Fragments arranged by size:
2817 2445 248
BbvCI CC'TCA_GC
Cuts at: 388 388
Size: 5510
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 579 613 1015 1049 90
Size: 34 455 34 402 34 4551
Fragments arranged by size:
4551 455 402 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 1249 1332 3408 4169 5510 1249
Size: 83 2076 761 1341 1249
Fragments arranged by size:
2076 1341 1249 761 83
BclI T'GATC_A
Cuts at: 2102 3030 2102
Size: 928 4582
BfrBI ATG'CAT
Cuts at: 1228 2008 1228
Size: 780 4730
BglII A'GATC_T
Cuts at: 1733 1907 3025 5062 1733
Size: 174 1118 2037 2181
Fragments arranged by size:
2181 2037 1118 174
BlpI GC'TnA_GC
Cuts at: 2622 2622
Size: 5510
Bme1580I G_kGCm'C
Cuts at: 120 592 950 1594 1928 1993 2400 3135 3228
Size: 472 358 644 334 65 407 735 93
Cuts at: 3228 4284 120
Size: 1056 1346
Fragments arranged by size:
1346 1056 735 644 472 407 358 334
93 65
BmrI ACTGGGnnnn_n'
Cuts at: 2201 2722 2803 3138 5282 2201
Size: 521 81 335 2144 2429
Fragments arranged by size:
2429 2144 521 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3877 3877
Size: 5510
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 853 1486 853
Size: 633 4877
Bpu10I CC'TnA_GC
Cuts at: 388 1797 388
Size: 1409 4101
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2686 3565 4057 4355 2686
Size: 879 492 298 3841
Fragments arranged by size:
3841 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 184 5214 184
Size: 5030 480
BsaAI yAC'GTr
Cuts at: 1493 2863 1493
Size: 1370 4140
BsaBI GATnn'nnATC
Cuts at: 6 1286 3024 5061 6
Size: 1280 1738 2037 455
Fragments arranged by size:
2037 1738 1280 455
BsaHI Gr'CG_yC
Cuts at: 124 422 2941 3189 3891 5387 124
Size: 298 2519 248 702 1496 247
Fragments arranged by size:
2519 1496 702 298 248 247
BsaWI w'CCGG_w
Cuts at: 578 632 1241 2357 2699 3220 3898 4172 4319
Size: 54 609 1116 342 521 678 274 147
Cuts at: 4319 4730 578
Size: 411 1358
Fragments arranged by size:
1358 1116 678 609 521 411 342 274
147 54
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 968 998 1414 1444 1690 1720 1750 1780 2230
Size: 30 416 30 246 30 30 30 450
Cuts at: 2230 2260 968
Size: 30 4218
Fragments arranged by size:
4218 450 416 246 30 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 390 619 390
Size: 229 5281
BseYI C'CCAG_C
Cuts at: 351 416 1408 2935 3693 4270 4766 351
Size: 65 992 1527 758 577 496 1095
Fragments arranged by size:
1527 1095 992 758 577 496 65
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1006 1473 1593 1692 1006
Size: 467 120 99 4824
Fragments arranged by size:
4824 467 120 99
BsiEI CG_ry'CG
Cuts at: 104 1444 2358 3052 3098 4306 104
Size: 1340 914 694 46 1208 1308
Fragments arranged by size:
1340 1308 1208 914 694 46
BsiHKAI G_wGCw'C
Cuts at: 256 1708 2204 2524 3302 3492 4284 256
Size: 1452 496 320 778 190 792 1482
Fragments arranged by size:
1482 1452 792 778 496 320 190
BsmI GAATG_Cn'
Cuts at: 1831 1831
Size: 5510
Bsp1286I G_dGCh'C
Cuts at: 120 152 256 352 409 592 950 1594 1708
Size: 32 104 96 57 183 358 644 114
Cuts at: 1708 1928 1993 2204 2400 2524 3135 3228 3302
Size: 220 65 211 196 124 611 93 74
Cuts at: 3302 3492 3554 4284 120
Size: 190 62 730 1346
Fragments arranged by size:
1346 730 644 611 358 220 211 196
190 183 124 114 104 96 93 74
65 62 57 32
BspEI T'CCGG_A
Cuts at: 578 4730 578
Size: 4152 1358
BspHI T'CATG_A
Cuts at: 2180 4686 2180
Size: 2506 3004
BspMI ACCTGCnnnn'nnnn_
Cuts at: 166 1315 1831 2005 2083 2560 2774 3076 3457
Size: 1149 516 174 78 477 214 302 381
Cuts at: 3457 166
Size: 2219
Fragments arranged by size:
2219 1149 516 477 381 302 214 174
78
BsrBI CCG'CTC
Cuts at: 50 1544 3802 3856 5340 5505 50
Size: 1494 2258 54 1484 165 55
Fragments arranged by size:
2258 1494 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3422 3422
Size: 5510
BsrFI r'CCGG_y
Cuts at: 1443 3508 3689 3898 1443
Size: 2065 181 209 3055
Fragments arranged by size:
3055 2065 209 181
BsrGI T'GTAC_A
Cuts at: 1139 1139
Size: 5510
BssHII G'CGCG_C
Cuts at: 155 155
Size: 5510
BssSI C'ACGA_G
Cuts at: 3781 4139 3781
Size: 358 5152
BstAPI GCAn_nnn'nTGC
Cuts at: 1598 1703 2887 1598
Size: 105 1184 4221
Fragments arranged by size:
4221 1184 105
BstBI TT'CG_AA
Cuts at: 3871 3871
Size: 5510
BstEII G'GTnAC_C
Cuts at: 1476 1476
Size: 5510
BstXI CCAn_nnnn'nTGG
Cuts at: 1047 2280 1047
Size: 1233 4277
BstYI r'GATC_y
Cuts at: 1 1281 1733 1907 2535 3002 3025 3360 3606
Size: 1280 452 174 628 467 23 335 246
Cuts at: 3606 4618 4704 4716 5062 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1280 1012 628 467 452 449 346 335
246 174 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 373 845 1277 2233 373
Size: 472 432 956 3650
Fragments arranged by size:
3650 956 472 432
BtgI C'CryG_G
Cuts at: 112 277 1803 112
Size: 165 1526 3819
Fragments arranged by size:
3819 1526 165
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 939 3460 3641 3804 939
Size: 2521 181 163 2645
Fragments arranged by size:
2645 2521 181 163
BtsI GCAGTG_nn'
Cuts at: 913 1031 1121 1815 1948 2753 913
Size: 118 90 694 133 805 3670
Fragments arranged by size:
3670 805 694 133 118 90
DraI TTT'AAA
Cuts at: 2509 2509
Size: 5510
DraIII CAC_nnn'GTG
Cuts at: 1598 2454 1598
Size: 856 4654
DrdI GACnn_nn'nnGTC
Cuts at: 3216 4074 3216
Size: 858 4652
EaeI y'GGCC_r
Cuts at: 999 1441 1902 3095 3269 3660 3687 5032 999
Size: 442 461 1193 174 391 27 1345 1477
Fragments arranged by size:
1477 1345 1193 461 442 391 174 27
EagI C'GGCC_G
Cuts at: 1441 3095 1441
Size: 1654 3856
EarI CTCTTCn'nnn_
Cuts at: 1297 3533 3743 1297
Size: 2236 210 3064
Fragments arranged by size:
3064 2236 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 748 3718 4028 4174 5320 748
Size: 2970 310 146 1146 938
Fragments arranged by size:
2970 1146 938 310 146
EcoICRI GAG'CTC
Cuts at: 2202 2522 2202
Size: 320 5190
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 853 1375 1486 3335 3767 4514 4782 853
Size: 522 111 1849 432 747 268 1581
Fragments arranged by size:
1849 1581 747 522 432 268 111
EcoNI CCTnn'n_nnAGG
Cuts at: 1015 1773 1015
Size: 758 4752
EcoO109I rG'GnC_Cy
Cuts at: 117 1547 1964 2396 2649 117
Size: 1430 417 432 253 2978
Fragments arranged by size:
2978 1430 432 417 253
EcoRI G'AATT_C
Cuts at: 2514 2514
Size: 5510
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 498 582 3529 498
Size: 84 2947 2479
Fragments arranged by size:
2947 2479 84
FspI TGC'GCA
Cuts at: 3291 3291
Size: 5510
HaeII r_GCGC'y
Cuts at: 127 1529 2944 3192 4214 5193 127
Size: 1402 1415 248 1022 979 444
Fragments arranged by size:
1415 1402 1022 979 444 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1038 1042 1070 1074 1180 1212 1306 1338 1357
Size: 4 28 4 106 32 94 32 19
Cuts at: 1357 1389 1750 1782 2015 2047 3352 3382 3384
Size: 32 361 32 233 32 1305 30 2
Cuts at: 3384 3414 1038
Size: 30 3134
Fragments arranged by size:
3134 1305 361 233 106 94 32 32
32 32 32 30 30 28 19 4
4 2
HincII GTy'rAC
Cuts at: 2549 2549
Size: 5510
KasI G'GCGC_C
Cuts at: 123 2940 3188 123
Size: 2817 248 2445
Fragments arranged by size:
2817 2445 248
KpnI G_GTAC'C
Cuts at: 699 2530 699
Size: 1831 3679
MluI A'CGCG_T
Cuts at: 4943 4943
Size: 5510
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1064 2243 4181 4365 1064
Size: 1179 1938 184 2209
Fragments arranged by size:
2209 1938 1179 184
MscI TGG'CCA
Cuts at: 1001 1904 3271 1001
Size: 903 1367 3240
Fragments arranged by size:
3240 1367 903
MslI CAynn'nnrTG
Cuts at: 430 2110 2122 2194 3499 3626 430
Size: 1680 12 72 1305 127 2314
Fragments arranged by size:
2314 1680 1305 127 72 12
MspA1I CmG'CkG
Cuts at: 114 129 1676 1694 2621 2935 3295 4308 4553
Size: 15 1547 18 927 314 360 1013 245
Cuts at: 4553 114
Size: 1071
Fragments arranged by size:
1547 1071 1013 927 360 314 245 18
15
NaeI GCC'GGC
Cuts at: 3691 3691
Size: 5510
NarI GG'CG_CC
Cuts at: 124 2941 3189 124
Size: 2817 248 2445
Fragments arranged by size:
2817 2445 248
NcoI C'CATG_G
Cuts at: 1803 1803
Size: 5510
NdeI CA'TA_TG
Cuts at: 187 187
Size: 5510
NgoMIV G'CCGG_C
Cuts at: 3689 3689
Size: 5510
NsiI A_TGCA'T
Cuts at: 1230 2010 1230
Size: 780 4730
NspI r_CATG'y
Cuts at: 1429 1889 2012 2563 3063 3970 1429
Size: 460 123 551 500 907 2969
Fragments arranged by size:
2969 907 551 500 460 123
PciI A'CATG_T
Cuts at: 3966 3966
Size: 5510
PflMI CCAn_nnn'nTGG
Cuts at: 997 2248 997
Size: 1251 4259
PfoI T'CCnGG_A
Cuts at: 826 1454 826
Size: 628 4882
PmeI GTTT'AAAC
Cuts at: 2509 2509
Size: 5510
PmlI CAC'GTG
Cuts at: 1493 1493
Size: 5510
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4649 4681 4649
Size: 32 5478
PpuMI rG'GwC_Cy
Cuts at: 1547 1547
Size: 5510
PshAI GACnn'nnGTC
Cuts at: 1552 1552
Size: 5510
PspOMI G'GGCC_C
Cuts at: 116 2396 116
Size: 2280 3230
PstI C_TGCA'G
Cuts at: 414 1504 1693 1915 2557 3242 414
Size: 1090 189 222 642 685 2682
Fragments arranged by size:
2682 1090 685 642 222 189
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5510
PvuII CAG'CTG
Cuts at: 1694 2935 3295 1694
Size: 1241 360 3909
Fragments arranged by size:
3909 1241 360
RsrII CG'GwC_CG
Cuts at: 3705 3705
Size: 5510
SacI G_AGCT'C
Cuts at: 2204 2524 2204
Size: 320 5190
SacII CC_GC'GG
Cuts at: 115 115
Size: 5510
SalI G'TCGA_C
Cuts at: 2547 2547
Size: 5510
SapI GCTCTTCn'nnn_
Cuts at: 3533 3743 3533
Size: 210 5300
SbfI CC_TGCA'GG
Cuts at: 2557 2557
Size: 5510
ScaI AGT'ACT
Cuts at: 866 5027 866
Size: 4161 1349
SexAI A'CCwGG_T
Cuts at: 1594 1594
Size: 5510
SfcI C'TryA_G
Cuts at: 33 410 490 1500 1689 1911 2553 3238 4231
Size: 377 80 1010 189 222 642 685 993
Cuts at: 4231 4422 33
Size: 191 1121
Fragments arranged by size:
1121 1010 993 685 642 377 222 191
189 80
SfoI GGC'GCC
Cuts at: 125 2942 3190 125
Size: 2817 248 2445
Fragments arranged by size:
2817 2445 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5510
SmaI CCC'GGG
Cuts at: 2532 2532
Size: 5510
SmlI C'TyrA_G
Cuts at: 2665 3580 4072 4334 2665
Size: 915 492 262 3841
Fragments arranged by size:
3841 915 492 262
SpeI A'CTAG_T
Cuts at: 67 193 67
Size: 126 5384
SphI G_CATG'C
Cuts at: 2012 2563 3063 2012
Size: 551 500 4459
Fragments arranged by size:
4459 551 500
SspI AAT'ATT
Cuts at: 1206 1206
Size: 5510
StuI AGG'CCT
Cuts at: 980 980
Size: 5510
StyI C'CwwG_G
Cuts at: 1803 2168 2644 2998 1803
Size: 365 476 354 4315
Fragments arranged by size:
4315 476 365 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 251 2100 3093 3226 3685 3894 251
Size: 1849 993 133 459 209 1867
Fragments arranged by size:
1867 1849 993 459 209 133
TatI w'GTAC_w
Cuts at: 527 864 1028 1139 5025 527
Size: 337 164 111 3886 1012
Fragments arranged by size:
3886 1012 337 164 111
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 968 1000 1160 1192 1816 1848 2230 2262 5027
Size: 32 160 32 624 32 382 32 2765
Cuts at: 5027 5059 968
Size: 32 1419
Fragments arranged by size:
2765 1419 624 382 160 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 2176 3307 2176
Size: 1131 4379
XbaI T'CTAG_A
Cuts at: 2541 2541
Size: 5510
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 245 2451 3005 245
Size: 2206 554 2750
Fragments arranged by size:
2750 2206 554
XmaI C'CCGG_G
Cuts at: 2530 2530
Size: 5510
ZraI GAC'GTC
Cuts at: 423 423
Size: 5510
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AgeI
AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbeI BbvCI BcgI
BciVI BclI BfrBI BglII BlpI Bme1580I BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NsiI
NspI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PspOMI PstI PvuI PvuII RsrII SacI SacII
SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI
SmaI SmlI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI ZraI
Enzymes that do not cut:
AfeI AflII AhdI AloI AscI AvrII BbsI BglI
BmgBI BmtI BsiWI BsmBI BstZ17I ClaI CspCI EcoRV
FseI FspAI HindIII HpaI MfeI NheI NotI NruI
PacI PasI PsiI PspXI PsrI SanDI SfiI SgrAI
SnaBI SrfI SwaI XhoI XmnI