Restriction map for
pF1KB5730
(Circular) (Six-base) MAPSORT of: pF1KB5730.seq Check: 538 from: 1 to: 5309
pF1KB5730 5309 bp
With 182 enzymes: SgfI *
October 7, 2009 14:17 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 148 1524 148
Size: 1376 3933
AatII G_ACGT'C
Cuts at: 1724 1724
Size: 5309
AccI GT'mk_AC
Cuts at: 2347 2347
Size: 5309
Acc65I G'GTAC_C
Cuts at: 2325 2325
Size: 5309
AclI AA'CG_TT
Cuts at: 5149 5149
Size: 5309
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 402 711 1535 1585 1960 3134 3566 4313 4581
Size: 309 824 50 375 1174 432 747 268
Cuts at: 4581 402
Size: 1130
Fragments arranged by size:
1174 1130 824 747 432 375 309 268
50
AflIII A'CryG_T
Cuts at: 1142 2661 3765 4742 1142
Size: 1519 1104 977 1709
Fragments arranged by size:
1709 1519 1104 977
AgeI A'CCGG_T
Cuts at: 3697 3697
Size: 5309
AhdI GACnn_n'nnGTC
Cuts at: 1056 1056
Size: 5309
AleI CACnn'nnGTG
Cuts at: 394 394
Size: 5309
AlwNI CAG_nnn'CTG
Cuts at: 535 604 963 1281 2291 2614 4181 535
Size: 69 359 318 1010 323 1567 1663
Fragments arranged by size:
1663 1567 1010 359 323 318 69
ApaI G_GGCC'C
Cuts at: 1563 2219 1563
Size: 656 4653
ApaLI G'TGCA_C
Cuts at: 2258 4079 2258
Size: 1821 3488
ApoI r'AATT_y
Cuts at: 516 2313 4662 4918 516
Size: 1797 2349 256 907
Fragments arranged by size:
2349 1797 907 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5309
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5309
AvaI C'yCGr_G
Cuts at: 1404 2329 1404
Size: 925 4384
AvrII C'CTAG_G
Cuts at: 738 738
Size: 5309
BamHI G'GATC_C
Cuts at: 2334 2334
Size: 5309
BanI G'GyrC_C
Cuts at: 524 2072 2325 2739 2987 3022 524
Size: 1548 253 414 248 35 2811
Fragments arranged by size:
2811 1548 414 253 248 35
BanII G_rGCy'C
Cuts at: 1099 1367 1563 2219 2323 3353 1099
Size: 268 196 656 104 1030 3055
Fragments arranged by size:
3055 1030 656 268 196 104
BbeI G_GCGC'C
Cuts at: 2743 2991 2743
Size: 248 5061
BbvCI CC'TCA_GC
Cuts at: 2107 2221 2107
Size: 114 5195
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1341 1375 1602 1636 1341
Size: 34 227 34 5014
Fragments arranged by size:
5014 227 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 570 3207 3968 5309 570
Size: 2637 761 1341 570
Fragments arranged by size:
2637 1341 761 570
BclI T'GATC_A
Cuts at: 2829 2829
Size: 5309
BglII A'GATC_T
Cuts at: 830 842 2120 2824 4861 830
Size: 12 1278 704 2037 1278
Fragments arranged by size:
2037 1278 1278 704 12
BlpI GC'TnA_GC
Cuts at: 1270 2421 1270
Size: 1151 4158
Bme1580I G_kGCm'C
Cuts at: 990 1563 2219 2262 2934 3027 4083 990
Size: 573 656 43 672 93 1056 2216
Fragments arranged by size:
2216 1056 672 656 573 93 43
BmgBI CAC'GTC
Cuts at: 1125 1125
Size: 5309
BmrI ACTGGGnnnn_n'
Cuts at: 2521 2602 2937 5081 2521
Size: 81 335 2144 2749
Fragments arranged by size:
2749 2144 335 81
BmtI G_CTAG'C
Cuts at: 2035 2035
Size: 5309
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1118 3676 1118
Size: 2558 2751
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 179 198 526 994 1214 1903 2268 179
Size: 19 328 468 220 689 365 3220
Fragments arranged by size:
3220 689 468 365 328 220 19
Bpu10I CC'TnA_GC
Cuts at: 1093 1633 2107 2221 1093
Size: 540 474 114 4181
Fragments arranged by size:
4181 540 474 114
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 793 1012 2485 3364 3856 4154 793
Size: 219 1473 879 492 298 1948
Fragments arranged by size:
1948 1473 879 492 298 219
BsaI GGTCTCn'nnnn_
Cuts at: 439 890 1489 5013 439
Size: 451 599 3524 735
Fragments arranged by size:
3524 735 599 451
BsaAI yAC'GTr
Cuts at: 2263 2662 2263
Size: 399 4910
BsaBI GATnn'nnATC
Cuts at: 6 2823 4860 6
Size: 2817 2037 455
Fragments arranged by size:
2817 2037 455
BsaHI Gr'CG_yC
Cuts at: 1721 2740 2988 3690 5186 1721
Size: 1019 248 702 1496 1844
Fragments arranged by size:
1844 1496 1019 702 248
BsaWI w'CCGG_w
Cuts at: 1643 2498 3019 3697 3971 4118 4529 1643
Size: 855 521 678 274 147 411 2423
Fragments arranged by size:
2423 855 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1212 1242 1212
Size: 30 5279
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 556 643 908 1174 1357 1507 1765 556
Size: 87 265 266 183 150 258 4100
Fragments arranged by size:
4100 266 265 258 183 150 87
BseYI C'CCAG_C
Cuts at: 876 888 2095 2114 2161 2232 2734 3492 4069
Size: 12 1207 19 47 71 502 758 577
Cuts at: 4069 4565 876
Size: 496 1620
Fragments arranged by size:
1620 1207 758 577 502 496 71 47
19 12
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 390 390
Size: 5309
BsiEI CG_ry'CG
Cuts at: 104 2851 2897 4105 104
Size: 2747 46 1208 1308
Fragments arranged by size:
2747 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 193 1411 2262 2271 2323 3101 3291 4083 193
Size: 1218 851 9 52 778 190 792 1419
Fragments arranged by size:
1419 1218 851 792 778 190 52 9
BsmI GAATG_Cn'
Cuts at: 2016 2016
Size: 5309
Bsp1286I G_dGCh'C
Cuts at: 193 990 1099 1367 1411 1563 2219 2262 2271
Size: 797 109 268 44 152 656 43 9
Cuts at: 2271 2323 2934 3027 3101 3291 3353 4083 193
Size: 52 611 93 74 190 62 730 1419
Fragments arranged by size:
1419 797 730 656 611 268 190 152
109 93 74 62 52 44 43 9
BspEI T'CCGG_A
Cuts at: 4529 4529
Size: 5309
BspHI T'CATG_A
Cuts at: 4485 4485
Size: 5309
BspMI ACCTGCnnnn'nnnn_
Cuts at: 148 1524 2359 2573 2875 3256 148
Size: 1376 835 214 302 381 2201
Fragments arranged by size:
2201 1376 835 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3601 3655 5139 5304 50
Size: 3551 54 1484 165 55
Fragments arranged by size:
3551 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3221 3221
Size: 5309
BsrFI r'CCGG_y
Cuts at: 3307 3488 3697 3307
Size: 181 209 4919
Fragments arranged by size:
4919 209 181
BssSI C'ACGA_G
Cuts at: 1111 1849 3580 3938 1111
Size: 738 1731 358 2482
Fragments arranged by size:
2482 1731 738 358
BstAPI GCAn_nnn'nTGC
Cuts at: 2266 2686 2266
Size: 420 4889
BstBI TT'CG_AA
Cuts at: 2130 3670 2130
Size: 1540 3769
BstEII G'GTnAC_C
Cuts at: 1218 1218
Size: 5309
BstXI CCAn_nnnn'nTGG
Cuts at: 160 322 160
Size: 162 5147
BstYI r'GATC_y
Cuts at: 1 357 830 842 2120 2334 2801 2824 3159
Size: 356 473 12 1278 214 467 23 335
Cuts at: 3159 3405 4417 4503 4515 4861 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1278 1012 473 467 449 356 346 335
246 214 86 23 12 12
BtgI C'CryG_G
Cuts at: 107 212 229 2076 2211 107
Size: 105 17 1847 135 3205
Fragments arranged by size:
3205 1847 135 105 17
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2022 3259 3440 3603 2022
Size: 1237 181 163 3728
Fragments arranged by size:
3728 1237 181 163
BtsI GCAGTG_nn'
Cuts at: 242 1436 2552 242
Size: 1194 1116 2999
Fragments arranged by size:
2999 1194 1116
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1681 1716 1681
Size: 35 5274
DraI TTT'AAA
Cuts at: 2308 2308
Size: 5309
DraIII CAC_nnn'GTG
Cuts at: 2266 2266
Size: 5309
DrdI GACnn_nn'nnGTC
Cuts at: 3015 3873 3015
Size: 858 4451
EaeI y'GGCC_r
Cuts at: 239 711 2894 3068 3459 3486 4831 239
Size: 472 2183 174 391 27 1345 717
Fragments arranged by size:
2183 1345 717 472 391 174 27
EagI C'GGCC_G
Cuts at: 2894 2894
Size: 5309
EarI CTCTTCn'nnn_
Cuts at: 128 194 927 1006 3332 3542 128
Size: 66 733 79 2326 210 1895
Fragments arranged by size:
2326 1895 733 210 79 66
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3517 3827 3973 5119 3517
Size: 310 146 1146 3707
Fragments arranged by size:
3707 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2321 2321
Size: 5309
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 179 198 402 526 711 994 1214 1535 1585
Size: 19 204 124 185 283 220 321 50
Cuts at: 1585 1903 1960 2268 3134 3566 4313 4581 179
Size: 318 57 308 866 432 747 268 907
Fragments arranged by size:
907 866 747 432 321 318 308 283
268 220 204 185 124 57 50 19
EcoNI CCTnn'n_nnAGG
Cuts at: 432 2241 432
Size: 1809 3500
EcoO109I rG'GnC_Cy
Cuts at: 742 968 1560 2216 2448 742
Size: 226 592 656 232 3603
Fragments arranged by size:
3603 656 592 232 226
EcoRI G'AATT_C
Cuts at: 516 2313 516
Size: 1797 3512
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3328 3328
Size: 5309
FspI TGC'GCA
Cuts at: 3090 3090
Size: 5309
HaeII r_GCGC'y
Cuts at: 1829 1947 2743 2991 4013 4992 1829
Size: 118 796 248 1022 979 2146
Fragments arranged by size:
2146 1022 979 796 248 118
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1042 1074 1212 1244 3151 3181 3183 3213 1042
Size: 32 138 32 1907 30 2 30 3138
Fragments arranged by size:
3138 1907 138 32 32 30 30 2
HincII GTy'rAC
Cuts at: 2348 2348
Size: 5309
KasI G'GCGC_C
Cuts at: 2739 2987 2739
Size: 248 5061
KpnI G_GTAC'C
Cuts at: 2329 2329
Size: 5309
MluI A'CGCG_T
Cuts at: 4742 4742
Size: 5309
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1803 3980 4164 1803
Size: 2177 184 2948
Fragments arranged by size:
2948 2177 184
MscI TGG'CCA
Cuts at: 241 713 3070 241
Size: 472 2357 2480
Fragments arranged by size:
2480 2357 472
MslI CAynn'nnrTG
Cuts at: 348 394 1084 3298 3425 348
Size: 46 690 2214 127 2232
Fragments arranged by size:
2232 2214 690 127 46
MspA1I CmG'CkG
Cuts at: 876 981 1768 2255 2291 2420 2734 3094 4107
Size: 105 787 487 36 129 314 360 1013
Cuts at: 4107 4352 876
Size: 245 1833
Fragments arranged by size:
1833 1013 787 487 360 314 245 129
105 36
NaeI GCC'GGC
Cuts at: 3490 3490
Size: 5309
NarI GG'CG_CC
Cuts at: 2740 2988 2740
Size: 248 5061
NcoI C'CATG_G
Cuts at: 107 229 2076 107
Size: 122 1847 3340
Fragments arranged by size:
3340 1847 122
NgoMIV G'CCGG_C
Cuts at: 3488 3488
Size: 5309
NheI G'CTAG_C
Cuts at: 2031 2031
Size: 5309
NspI r_CATG'y
Cuts at: 1146 2362 2862 3769 1146
Size: 1216 500 907 2686
Fragments arranged by size:
2686 1216 907 500
PciI A'CATG_T
Cuts at: 1142 3765 1142
Size: 2623 2686
PflMI CCAn_nnn'nTGG
Cuts at: 535 1052 1125 1506 535
Size: 517 73 381 4338
Fragments arranged by size:
4338 517 381 73
PfoI T'CCnGG_A
Cuts at: 362 1250 1445 1919 362
Size: 888 195 474 3752
Fragments arranged by size:
3752 888 474 195
PmeI GTTT'AAAC
Cuts at: 2308 2308
Size: 5309
PmlI CAC'GTG
Cuts at: 2263 2263
Size: 5309
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4448 4480 4448
Size: 32 5277
PpuMI rG'GwC_Cy
Cuts at: 742 742
Size: 5309
PspOMI G'GGCC_C
Cuts at: 1559 2215 1559
Size: 656 4653
PstI C_TGCA'G
Cuts at: 600 1193 1535 1625 2019 2048 2356 3041 600
Size: 593 342 90 394 29 308 685 2868
Fragments arranged by size:
2868 685 593 394 342 308 90 29
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5309
PvuII CAG'CTG
Cuts at: 876 1768 2734 3094 876
Size: 892 966 360 3091
Fragments arranged by size:
3091 966 892 360
RsrII CG'GwC_CG
Cuts at: 3504 3504
Size: 5309
SacI G_AGCT'C
Cuts at: 2323 2323
Size: 5309
SalI G'TCGA_C
Cuts at: 2346 2346
Size: 5309
SapI GCTCTTCn'nnn_
Cuts at: 128 1006 3332 3542 128
Size: 878 2326 210 1895
Fragments arranged by size:
2326 1895 878 210
SbfI CC_TGCA'GG
Cuts at: 2356 2356
Size: 5309
ScaI AGT'ACT
Cuts at: 4826 4826
Size: 5309
SfcI C'TryA_G
Cuts at: 33 596 1189 1531 1621 2015 2044 2352 3037
Size: 563 593 342 90 394 29 308 685
Cuts at: 3037 4030 4221 33
Size: 993 191 1121
Fragments arranged by size:
1121 993 685 593 563 394 342 308
191 90 29
SfoI GGC'GCC
Cuts at: 2741 2989 2741
Size: 248 5061
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5309
SmaI CCC'GGG
Cuts at: 2331 2331
Size: 5309
SmlI C'TyrA_G
Cuts at: 808 1027 2464 3379 3871 4133 808
Size: 219 1437 915 492 262 1984
Fragments arranged by size:
1984 1437 915 492 262 219
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5309
SphI G_CATG'C
Cuts at: 2362 2862 2362
Size: 500 4809
SspI AAT'ATT
Cuts at: 1132 1132
Size: 5309
StuI AGG'CCT
Cuts at: 1396 1396
Size: 5309
StyI C'CwwG_G
Cuts at: 107 229 738 1714 2076 2443 2797 107
Size: 122 509 976 362 367 354 2619
Fragments arranged by size:
2619 976 509 367 362 354 122
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 542 1320 2892 3025 3484 3693 542
Size: 778 1572 133 459 209 2158
Fragments arranged by size:
2158 1572 778 459 209 133
TatI w'GTAC_w
Cuts at: 4824 4824
Size: 5309
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 378 410 758 790 938 970 4826 4858 378
Size: 32 348 32 148 32 3856 32 829
Fragments arranged by size:
3856 829 348 148 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 3106 3106
Size: 5309
XbaI T'CTAG_A
Cuts at: 2340 2340
Size: 5309
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2804 2804
Size: 5309
XmaI C'CCGG_G
Cuts at: 2329 2329
Size: 5309
XmnI GAAnn'nnTTC
Cuts at: 550 1432 550
Size: 882 4427
ZraI GAC'GTC
Cuts at: 1722 1722
Size: 5309
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AgeI
AhdI AleI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI AvrII BamHI BanI BanII BbeI BbvCI BcgI
BciVI BclI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NheI NspI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PspOMI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AflII AlfI AloI AscI BaeI BbsI BfrBI
BglI BsiWI BsmBI BsrGI BssHII BstZ17I Bsu36I ClaI
EcoRV FseI FspAI HindIII HpaI MfeI NdeI NotI
NruI NsiI PacI PasI PshAI PsiI PspXI PsrI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SwaI
XhoI