Restriction map for
pF1KB5713
(Circular) (Six-base) MAPSORT of: pF1KB5713.seq Check: 8636 from: 1 to: 5468
pF1KB5713 5468 bp
With 182 enzymes: SgfI *
March 2, 2010 14:26 ..
AccI GT'mk_AC
Cuts at: 902 1968 2506 902
Size: 1066 538 3864
Fragments arranged by size:
3864 1066 538
Acc65I G'GTAC_C
Cuts at: 2484 2484
Size: 5468
AclI AA'CG_TT
Cuts at: 5308 5308
Size: 5468
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 557 602 1568 2379 3293 3725 4472 4740 557
Size: 45 966 811 914 432 747 268 1285
Fragments arranged by size:
1285 966 914 811 747 432 268 45
AflIII A'CryG_T
Cuts at: 255 2820 3924 4901 255
Size: 2565 1104 977 822
Fragments arranged by size:
2565 1104 977 822
AgeI A'CCGG_T
Cuts at: 3856 3856
Size: 5468
AleI CACnn'nnGTG
Cuts at: 2332 2332
Size: 5468
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 984 984
Size: 5468
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2135 2167 2135
Size: 32 5436
AlwNI CAG_nnn'CTG
Cuts at: 408 2120 2773 4340 408
Size: 1712 653 1567 1536
Fragments arranged by size:
1712 1567 1536 653
ApaLI G'TGCA_C
Cuts at: 2335 4238 2335
Size: 1903 3565
ApoI r'AATT_y
Cuts at: 297 719 793 1097 1162 2206 2434 2472 4821
Size: 422 74 304 65 1044 228 38 2349
Cuts at: 4821 5077 297
Size: 256 688
Fragments arranged by size:
2349 1044 688 422 304 256 228 74
65 38
AseI AT'TA_AT
Cuts at: 20 20
Size: 5468
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5468
AvaI C'yCGr_G
Cuts at: 2488 2488
Size: 5468
BamHI G'GATC_C
Cuts at: 2493 2493
Size: 5468
BanI G'GyrC_C
Cuts at: 2484 2898 3146 3181 2484
Size: 414 248 35 4771
Fragments arranged by size:
4771 414 248 35
BanII G_rGCy'C
Cuts at: 145 231 2085 2482 3512 145
Size: 86 1854 397 1030 2101
Fragments arranged by size:
2101 1854 1030 397 86
BbeI G_GCGC'C
Cuts at: 2902 3150 2902
Size: 248 5220
BbsI GAAGACnn'nnnn_
Cuts at: 545 590 1310 1435 1556 1838 2037 2053 545
Size: 45 720 125 121 282 199 16 3960
Fragments arranged by size:
3960 720 282 199 125 121 45 16
BbvCI CC'TCA_GC
Cuts at: 218 1457 218
Size: 1239 4229
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 5434
BciVI GTATCCnnnnn_n'
Cuts at: 3366 4127 5468 3366
Size: 761 1341 3366
Fragments arranged by size:
3366 1341 761
BclI T'GATC_A
Cuts at: 2988 2988
Size: 5468
BglII A'GATC_T
Cuts at: 515 2983 5020 515
Size: 2468 2037 963
Fragments arranged by size:
2468 2037 963
BlpI GC'TnA_GC
Cuts at: 2580 2580
Size: 5468
Bme1580I G_kGCm'C
Cuts at: 2339 3093 3186 4242 2339
Size: 754 93 1056 3565
Fragments arranged by size:
3565 1056 754 93
BmrI ACTGGGnnnn_n'
Cuts at: 1749 1919 2680 2761 3096 5240 1749
Size: 170 761 81 335 2144 1977
Fragments arranged by size:
2144 1977 761 335 170 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 318 3835 318
Size: 3517 1951
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2221 2221
Size: 5468
Bpu10I CC'TnA_GC
Cuts at: 218 1457 218
Size: 1239 4229
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1273 1988 2644 3523 4015 4313 1273
Size: 715 656 879 492 298 2428
Fragments arranged by size:
2428 879 715 656 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 374 1501 5172 374
Size: 1127 3671 670
Fragments arranged by size:
3671 1127 670
BsaAI yAC'GTr
Cuts at: 2821 2821
Size: 5468
BsaBI GATnn'nnATC
Cuts at: 6 2982 5019 6
Size: 2976 2037 455
Fragments arranged by size:
2976 2037 455
BsaHI Gr'CG_yC
Cuts at: 2899 3147 3849 5345 2899
Size: 248 702 1496 3022
Fragments arranged by size:
3022 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2657 3178 3856 4130 4277 4688 2657
Size: 521 678 274 147 411 3437
Fragments arranged by size:
3437 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1622 1652 2135 2165 1622
Size: 30 483 30 4925
Fragments arranged by size:
4925 483 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1625 1837 1625
Size: 212 5256
BseYI C'CCAG_C
Cuts at: 165 1404 2893 3651 4228 4724 165
Size: 1239 1489 758 577 496 909
Fragments arranged by size:
1489 1239 909 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1175 1466 1175
Size: 291 5177
BsiEI CG_ry'CG
Cuts at: 104 3010 3056 4264 104
Size: 2906 46 1208 1308
Fragments arranged by size:
2906 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 2085 2339 2482 3260 3450 4242 2085
Size: 254 143 778 190 792 3311
Fragments arranged by size:
3311 792 778 254 190 143
BsmI GAATG_Cn'
Cuts at: 973 973
Size: 5468
Bsp1286I G_dGCh'C
Cuts at: 145 231 2085 2339 2482 3093 3186 3260 3450
Size: 86 1854 254 143 611 93 74 190
Cuts at: 3450 3512 4242 145
Size: 62 730 1371
Fragments arranged by size:
1854 1371 730 611 254 190 143 93
86 74 62
BspEI T'CCGG_A
Cuts at: 4688 4688
Size: 5468
BspHI T'CATG_A
Cuts at: 1416 1619 4644 1416
Size: 203 3025 2240
Fragments arranged by size:
3025 2240 203
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2518 2732 3034 3415 2518
Size: 214 302 381 4571
Fragments arranged by size:
4571 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3760 3814 5298 5463 50
Size: 3710 54 1484 165 55
Fragments arranged by size:
3710 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3380 3380
Size: 5468
BsrFI r'CCGG_y
Cuts at: 3466 3647 3856 3466
Size: 181 209 5078
Fragments arranged by size:
5078 209 181
BsrGI T'GTAC_A
Cuts at: 2388 2388
Size: 5468
BssSI C'ACGA_G
Cuts at: 3739 4097 3739
Size: 358 5110
BstAPI GCAn_nnn'nTGC
Cuts at: 1261 2845 1261
Size: 1584 3884
BstBI TT'CG_AA
Cuts at: 1301 3829 1301
Size: 2528 2940
BstXI CCAn_nnnn'nTGG
Cuts at: 836 2347 836
Size: 1511 3957
BstYI r'GATC_y
Cuts at: 1 515 2493 2960 2983 3318 3564 4576 4662
Size: 514 1978 467 23 335 246 1012 86
Cuts at: 4662 4674 5020 1
Size: 12 346 449
Fragments arranged by size:
1978 1012 514 467 449 346 335 246
86 23 12
BstZ17I GTA'TAC
Cuts at: 1969 1969
Size: 5468
BtgI C'CryG_G
Cuts at: 2055 2055
Size: 5468
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3418 3599 3762 3418
Size: 181 163 5124
Fragments arranged by size:
5124 181 163
BtsI GCAGTG_nn'
Cuts at: 707 1542 2711 707
Size: 835 1169 3464
Fragments arranged by size:
3464 1169 835
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 2037 2072 2037
Size: 35 5433
DraI TTT'AAA
Cuts at: 2211 2467 2211
Size: 256 5212
DrdI GACnn_nn'nnGTC
Cuts at: 3174 4032 3174
Size: 858 4610
EaeI y'GGCC_r
Cuts at: 826 1883 3053 3227 3618 3645 4990 826
Size: 1057 1170 174 391 27 1345 1304
Fragments arranged by size:
1345 1304 1170 1057 391 174 27
EagI C'GGCC_G
Cuts at: 3053 3053
Size: 5468
EarI CTCTTCn'nnn_
Cuts at: 457 1091 1148 1352 1799 3491 3701 457
Size: 634 57 204 447 1692 210 2224
Fragments arranged by size:
2224 1692 634 447 210 204 57
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3676 3986 4132 5278 3676
Size: 310 146 1146 3866
Fragments arranged by size:
3866 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2083 2480 2083
Size: 397 5071
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 557 602 1568 2221 2379 3293 3725 4472 4740
Size: 45 966 653 158 914 432 747 268
Cuts at: 4740 557
Size: 1285
Fragments arranged by size:
1285 966 914 747 653 432 268 158
45
EcoNI CCTnn'n_nnAGG
Cuts at: 2155 2155
Size: 5468
EcoO109I rG'GnC_Cy
Cuts at: 2607 2607
Size: 5468
EcoRI G'AATT_C
Cuts at: 2472 2472
Size: 5468
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1314 3487 1314
Size: 2173 3295
FspI TGC'GCA
Cuts at: 3249 3249
Size: 5468
HaeII r_GCGC'y
Cuts at: 124 172 2902 3150 4172 5151 124
Size: 48 2730 248 1022 979 441
Fragments arranged by size:
2730 1022 979 441 248 48
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 456 488 500 532 564 1066 1098 1354 1386
Size: 32 12 32 32 502 32 256 32
Cuts at: 1386 1622 1654 2079 2111 3310 3340 3342 3372
Size: 236 32 425 32 1199 30 2 30
Cuts at: 3372 456
Size: 2552
Fragments arranged by size:
2552 1199 502 425 256 236 32 32
32 32 32 32 32 30 30 12
2
HincII GTy'rAC
Cuts at: 600 2507 600
Size: 1907 3561
HindIII A'AGCT_T
Cuts at: 674 863 2404 674
Size: 189 1541 3738
Fragments arranged by size:
3738 1541 189
KasI G'GCGC_C
Cuts at: 2898 3146 2898
Size: 248 5220
KpnI G_GTAC'C
Cuts at: 2488 2488
Size: 5468
MluI A'CGCG_T
Cuts at: 4901 4901
Size: 5468
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 631 1162 1695 4139 4323 631
Size: 531 533 2444 184 1776
Fragments arranged by size:
2444 1776 533 531 184
MscI TGG'CCA
Cuts at: 1885 3229 1885
Size: 1344 4124
MslI CAynn'nnrTG
Cuts at: 896 1751 2332 2345 3457 3584 896
Size: 855 581 13 1112 127 2780
Fragments arranged by size:
2780 1112 855 581 127 13
MspA1I CmG'CkG
Cuts at: 2579 2893 3253 4266 4511 2579
Size: 314 360 1013 245 3536
Fragments arranged by size:
3536 1013 360 314 245
NaeI GCC'GGC
Cuts at: 3649 3649
Size: 5468
NarI GG'CG_CC
Cuts at: 2899 3147 2899
Size: 248 5220
NgoMIV G'CCGG_C
Cuts at: 3647 3647
Size: 5468
NspI r_CATG'y
Cuts at: 259 2521 3021 3928 259
Size: 2262 500 907 1799
Fragments arranged by size:
2262 1799 907 500
PciI A'CATG_T
Cuts at: 255 3924 255
Size: 3669 1799
PmeI GTTT'AAAC
Cuts at: 2467 2467
Size: 5468
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2135 2167 4607 4639 2135
Size: 32 2440 32 2964
Fragments arranged by size:
2964 2440 32 32
PsiI TTA'TAA
Cuts at: 1930 2384 1930
Size: 454 5014
PstI C_TGCA'G
Cuts at: 2159 2515 3200 2159
Size: 356 685 4427
Fragments arranged by size:
4427 685 356
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5468
PvuII CAG'CTG
Cuts at: 2893 3253 2893
Size: 360 5108
RsrII CG'GwC_CG
Cuts at: 3663 3663
Size: 5468
SacI G_AGCT'C
Cuts at: 2085 2482 2085
Size: 397 5071
SalI G'TCGA_C
Cuts at: 2505 2505
Size: 5468
SapI GCTCTTCn'nnn_
Cuts at: 3491 3701 3491
Size: 210 5258
SbfI CC_TGCA'GG
Cuts at: 2159 2515 2159
Size: 356 5112
ScaI AGT'ACT
Cuts at: 943 4985 943
Size: 4042 1426
SfcI C'TryA_G
Cuts at: 33 782 1655 2155 2511 3196 4189 4380 33
Size: 749 873 500 356 685 993 191 1121
Fragments arranged by size:
1121 993 873 749 685 500 356 191
SfoI GGC'GCC
Cuts at: 2900 3148 2900
Size: 248 5220
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5468
SmaI CCC'GGG
Cuts at: 2490 2490
Size: 5468
SmlI C'TyrA_G
Cuts at: 1288 2003 2623 3538 4030 4292 1288
Size: 715 620 915 492 262 2464
Fragments arranged by size:
2464 915 715 620 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5468
SphI G_CATG'C
Cuts at: 2521 3021 2521
Size: 500 4968
SspI AAT'ATT
Cuts at: 746 746
Size: 5468
StyI C'CwwG_G
Cuts at: 2602 2956 2602
Size: 354 5114
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1907 3051 3184 3643 3852 1907
Size: 1144 133 459 209 3523
Fragments arranged by size:
3523 1144 459 209 133
TatI w'GTAC_w
Cuts at: 233 941 2388 4983 233
Size: 708 1447 2595 718
Fragments arranged by size:
2595 1447 718 708
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 705 737 1371 1403 4985 5017 705
Size: 32 634 32 3582 32 1156
Fragments arranged by size:
3582 1156 634 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 3265 3265
Size: 5468
XbaI T'CTAG_A
Cuts at: 2499 2499
Size: 5468
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2963 2963
Size: 5468
XmaI C'CCGG_G
Cuts at: 2488 2488
Size: 5468
XmnI GAAnn'nnTTC
Cuts at: 1297 2246 1297
Size: 949 4519
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlfI
AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstXI
BstYI BstZ17I BtgI BtgZI BtsI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NgoMIV NspI PciI PmeI PpiI PsiI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI SspI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI ApaI AscI AvrII
BaeI BfrBI BglI BmgBI BmtI BsiWI BsmBI BssHII
BstEII Bsu36I ClaI DraIII EcoRV FseI FspAI HpaI
MfeI NcoI NdeI NheI NotI NruI NsiI PacI
PasI PflMI PfoI PmlI PpuMI PshAI PspOMI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
StuI SwaI XhoI ZraI